Abstract
In this study, antiproliferative and antioxidant activities of crude extracts (hexane, ethyl acetate and methanol) from leaves and stem of Chresta sphaerocephala DC., Asteraceae, were investigated. Antiproliferative activity was tested in vitro against ten human cancer cells and against VERO (no cancer cell). Antioxidant activities were determined using DPPH and ORAC-FL assays and the total phenolic content was estimated by Folin–Ciocalteu method. Hexane and ethyl acetate extracts (leaves and stem) exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 50.40 and 250 μg/ml. For VERO cell, TGI values were >250 μg/ml for all extracts, except to hexane extract of the stem (TGI 80.92 μg/ml). In an initial evaluation, ethyl acetate and methanol extracts (leaves and stem) have shown levels of phenolic compounds between 6.94 and 30.96 mg GAE/kg in Folin–Ciocalteu assay, DPPH free-radical scavenging activity with SC50 in the range of 75.22 and 400 μg/ml and antioxidant capacity between 290.08 and 1088 μmol TE/g of extract in ORAC-FL assay. HPLC-DAD and ESI-MS analysis allowed the identification of flavonoids in the methanol extract from the leaves of C. sphaerocephala. Three steroids and nine triterpenoids were identified in the bioactive hexane extracts using HRGC.
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Burda, S., Oleszek, W., 2001. Antioxidant and antiradical activities of flavonoids. J. Agric. Food Chem. 49. 2774–2779.
Farias, M.R., 2004. Avaliação da qualidade de materias-primas vegetais. In: Simões, C.M.O., Schenkel, E.P., Gosmann, G., Mello, J.C.P., Mentz, L.A., Petrovick, P.R. (Eds.), Farmacognosia: da planta ao medicamento., 5th ed. Editora da Universidade UFRGS/Editora da UFSC, Porto Alegre/Florianópolis, pp. 263–288.
Gobbo-Neto, L., Lopes, N.P., 2008. Online identification of chlorogenic acids, sesquiterpene lactones, and flavonoids in the Brazilian arnica Lychnophora ericoides Mart (Asteraceae) leaves by HPLC-DAD–MS and HPLC-DAD–MS/MS and a validated HPLC-DAD method for their simultaneous analysis. J. Agric. Food Chem. 56. 1193–1204.
Gurib-Fakim, A., 2006. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol. Asp. Med. 27. 1–93.
Haddad, R., Milagre, H.M.S., Catharino, R.R., Eberlin, M.N., 2008. Easy ambient sonicspray ionization mass spectrometry combined with thin-layer chromatography. Anal. Chem. 80. 2744–2750.
Huang, D., Ou, B., Prior, R.L., 2005. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53. 1841–1856.
Kasahara, Y., Kumaki, K., Katagiri, S., Yasukawa, K., Yamanouchi, S., Takido, M., Akihisa, T., Tamuta, T., 1994. Carthamiflos extract and its component, stigmasterol, inhibit tumour promotion in mouse skin two-stage carcinogenesis. Phytother. Res. 8. 327–331.
Kumar, S.V., Saravanan, D., Kumar, B., Jayakumar, A., 2014. An update on prodrugs from natural products. Asian Pac. J. Trop. Med. 7. S54–S59.
Maciel, M.A.M., Pinto, A.A., Veiga, V.F.J., 2002. Plantas medicinais: a necessidade de estudos multidisciplinares. Quim. Nova 25. 429–438.
Monks, A., Scudiero, D., Skehan, P., Shoemaker, R., Paull, K., Vistica, D., Hose, C., Langley, J., Cronise, P., Vaigro-Wolff, A., Gray-Goodrich, M., Campbell, H., Mayo, J., Boyd, M., 1991. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J. Natl. Cancer Inst. 83. 757–766.
Ou, B., Hampsch-Woodill, M., Prior, R.L., 2001. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J. Agric. Food Chem. 49. 4619–4626.
Piccinelli, A.L., De Simone, F., Passi, S., Rastrelli, L., 2004. Phenolic constituents and antioxidant activity of Wendita calysina leaves (burrito), a folk Paraguayan tea. J. Agric. Food Chem. 52. 5863–5868.
Prior, R.L., Hoang, H., Gu, L., Wu, X., Bacchiocca, M., Howard, L., Hampsch-Woodill, M., Huang, D., Ou, B., Jacob, R., 2003. Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORAC-FL)) of plasma and other biological and food samples. J. Agric. Food Chem. 51. 3273–3279.
Rishton, G.M., 2008. Natural products as a robust source of new drugs and drug leads: past successes and present day issues. Am. J. Cardiol. 101. S43–S49.
Robinson, H., 1999. In: Smithsonian Institution Press (Ed.), Generic and Subtribal Classification of American Vernonieae. Smithsonian Contributions to Botany, Washington, DC, pp. 9–12.
Romanova, D., Vacakova, A., Cipak, L., Ovesna, Z., Rauko, P., 2001. Study of antioxidant effect of apigenin, luteolin and quercetin by DNA protective method. Neoplasma 48. 104–107.
Sala, A., Recio, M.C., Schinella, G.R., Ma˜nez, S., Giner, R.M., Cerdá-Nicolas, M., Rios, J.L., 2003. Assessment of the anti-inflammatory activity and free radical scavenger activity of tiliroside. Eur. J. Pharmacol. 461. 153–161.
Saleem, M., 2009. Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett. 285. 109–115.
Salvador, M.J., Ferreira, E.O., Mertens-Talcott, S.U., Castro, W.V., Butterweck, V., Derendorf, H., Dias, D.A., 2006. Isolation and HPLC quantitative analysis of antioxidant flavonoids from Alternanthera tenella Colla. Z. Naturforsch. C 61. 19–25.
Salvador, M.J., Sartori, F.T., Sacilotto, A.C.B.C., Pral, E.M., Alfieri, S.C., Vichnewski, W., 2009. Bioactivity of flavonoids isolated from Lychnophora markgravii against Leishmania amazonensis amastigotes. Z. Naturforsch. C 64. 509.
Schinor, E.C., Salvador, M.J., Turatti, I.C., Zucchi, O.L.A.D., Dias, D.A., 2004. Comparison of classical and ultrasound-assisted extractions of steroids and triterpenoids from three Chresta spp. Ultrason. Sonochem. 11. 415–421.
Schinor, E.C., Salvador, M.J., Tomaz, J.C., Pral, E.M.F., Alfieri, S.C., Albuquerque, S., Dias, D.A., 2006. Biological activities and chemical composition of crude extracts from Chresta exsucca. Braz. J. Pharm. Sci. 42. 83–90.
Schinor, E.C., Salvador, M.J., Pral, E.M.F., Alfieri, S.C., Albuquerque, S., Dias, D.A., 2007a. Effect of extracts and isolated compounds from Chresta scapigera on viability of Leishmania amazonensis and Trypanossoma cruzi. Braz. J. Pharm. Sci. 43. 295–300.
Schinor, E.C., Salvador, M.J., Ito, I.Y., Dias, D.A., 2007b. Evaluation of the antimicrobial activity of crude extracts and isolated constituents from Chresta scapigera. Braz. J. Microbiol. 38. 145–149.
Shoemaker, R.H., 2006. The NCI60 human tumour cell line anticancer drug screen. Nat. Rev. Cancer 6. 813–823.
Shohreh, N., Mehrdad, H., MehdI, R., Heidar, A.T., 2008. DNA adducts with antioxidant flavonoids: morin, apigenin, and naringin. DNA Cell Biol. 27. 433–442.
Silva, A.A.R., Bezerra, M.M., Chaves, H.V., Pinto, V.P.T., Franco, E.S., Vieira, A.M., Araújo, E.B., Cunha, L.R., Leite, A.C.R., Maia, M.B.S., 2012. Protective effect of Chresta martii extract on ethanol-induced gastropathy depends on alpha-2 adrenoceptors pathways but not on nitric oxide, prostaglandins or opioids. J. Ethnopharmacol. 142. 206–212.
Wu, X., Beecher, G.R., Holden, J.M., Haytowitz, D.B., Gebhardt, S.E., Prior, R.L., 2004. Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. J. Agric. Food Chem. 52. 4026–4037.
Yasukawa, K., Takido, M., Matsumoto, T., Takeuchi, M., Nakagawa, S., 1991. Sterol and triterpene derivatives from plants inhibit the effects of tumour promoter and sitosterol and betulinic acid inhibit tumour in mouse skin two-stage carcinogenesis. Oncology 41. 72–76.
Acknowledgements
The authors are grateful to Dr. João Semir from Departamento de Biologia Vegetal/UNICAMP for plant identification; to Dr. Marcos Nogueira Eberlin from Laboratório Thomson de Espectrometria de Massas, Instituto de Química/UNICAMP for mass spectra; and to FAPESP, CAPES, CNPq and FAEPEX-UNICAMP for financial support.
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LSC and NLA carried out all experimental procedures of this study, data analysis and interpretation and drafted the manuscript. WRC assisted in antioxidant assays. ALTGR and JEC assisted in the antiproliferative biological assays. IBSC carried out ESI-MS analysis and assisted in spectra data interpretation. ECS, DAD and MJS conceived the study and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
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da Costa, L.S., Andreazza, N.L., Correa, W.R. et al. Antiproliferative activity, antioxidant capacity and chemical composition of extracts from the leaves and stem of Chresta sphaerocephala. Rev. Bras. Farmacogn. 25, 369–374 (2015). https://doi.org/10.1016/j.bjp.2015.04.005
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DOI: https://doi.org/10.1016/j.bjp.2015.04.005