Abstract
The aim of this study is to investigate the effect of Cecropia glaziovii Snethl, Urticaceae, extracts on the oral glucose tolerance curve, on glycemia in alloxan-induced diabetic rats and vasorelaxant effect after the extraction process, and to standardize the extractive solutions. The effects of the process variables and their interactions were calculated in relation to dry residue, pH, total phenolic results and chemical marker content. Furthermore, the effect of the extracts (400 mg/kg), chlorogenic (2 or 15mg/kg) and caffeic acids (2 mg/kg) were investigated on the oral glucose tolerance curve and on glycemia in alloxan-induced diabetic rats. Oral administration of ethanol extracts 4d20 and 8d20 significantly improved glucose tolerance in the hyperglycemic rats. Chlorogenic and caffeic acids, as well as the association of the compounds were able to significantly reduce glycemia after oral gavage treatments. On the other hand, the aqueous extracts did not alter the glycemia. The aqueous extracts (8020 and 9030) and only the higher dose of chlorogenic acid presented a significant effect on serum glucose lowering in diabetic rats. Additionally, the IC50 reveals that the ethanol extracts presented more potent vasodilator effects than the aqueous extracts in aortic rings. This study shows that C. glaziovii standardized extracts exhibits antihyperglycemic action, is able to improve glucose tolerance and has a potent vascular relaxing effect. These results are probably linked to concentrations of the main phenolic compounds of the extracts.
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Acknowledgments
The authors thank FAPESC (Grant Number 05798, edict 008/2006), CNPq and Ílio Montanari Jr. researcher at CPQBA.
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DPA (MSc student) contributed in running the laboratory work, chromatographic analysis, analysis of the data and drafted the paper. TCS contributed in drafting the manuscript. LHC and MAH contributed to biological studies. DS, ALGS, RMRV and FRMBS contributed to critical reading of the manuscript. AMC designed the study, supervised the laboratory work and contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.
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Arend, D.P., dos Santos, T.C., Cazarolli, L.H. et al. In vivo potential hypoglycemic and in vitro vasorelaxant effects of Cecropia glaziovii standardized extracts. Rev. Bras. Farmacogn. 25, 473–484 (2015). https://doi.org/10.1016/j.bjp.2015.05.010
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DOI: https://doi.org/10.1016/j.bjp.2015.05.010