Abstract
The present study reports a promising antioxidant protection by a recently developed micellar propolis formulation, against oxidative stress in in vitro and in vivo models of toxicity. The formulation, based on poplar propolis encapsulated in poly(ethylene oxide)-β-poly(propylene oxide)-β-poly(ethylene oxide) triblock copolymer (PEO26-PPO40-PEO26) micelles is characterized by small size (Dh = 20 nm), enhances aqueous solubility and good colloidal stability. In vitro, propolis-loaded PEO26-PPO40-PEO26 micelles (20–100 μg/ml) significantly increased the cell viability of human hepatoma HepG2 cells, subjected to H2O2-induced cell injury (0.1 mM, 1 h). Antioxidant activity and protection of the micellar propolis were evaluated in a model of carbon tetrachloride-induced hepatotoxicity in rats (10% CCl4 solution, 1.25 ml/kg, p.o.) by measurement of non-enzyme (malondialdehyde and glutathione) and enzyme (catalase and superoxide dismutase) biomarkers of oxidative stress. Clinic observations, hematological, biochemical parameters and histological analysis were also performed. In vivo, micellar propolis (20 mg/kg b.w., p.o., 14 days) ameliorated CCl4-induced acute liver injury in rats. The oral administration of micellar propolis significantly prevented serum transaminase increases, as well as brought the levels of malondialdehyde, glutathione, and antioxidant enzymes catalase and superoxide dismutase toward the controls levels. Therefore, PEO26-PPO40-PEO26 micelles could be considered as a promising oral delivery system of propolis against oxidative stress injury in liver cells.
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VT mainly contributed to conception, study design and interpretation of the results and writing of the manuscript, VB has substantial contribution in propolis preparation and chemical characterization, PP prepared and characterized the propolis loaded micellar nanoformulation and contributed to writing of the manuscript, DA and YY (PhD student) have a substantial contribution to acquisition of experimental in vitro and in vivo data (biochemical and hematology parameters), MKB contributed to no nenzyme biomarker analysis in vivo, RS and VV contributed in acquisition of experimental in vivo data and oxidative enzyme biomarker analysis, FO and AA have performed histopathology analysis and results interpretation, BT and KY contributed to the release studies, interpretation of the results and critical revision of the manuscript.
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Tzankova, V., Aluani, D., Yordanov, Y. et al. Micellar propolis nanoformulation of high antioxidant and hepatoprotective activity. Rev. Bras. Farmacogn. 29, 364–372 (2019). https://doi.org/10.1016/j.bjp.2018.12.006
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DOI: https://doi.org/10.1016/j.bjp.2018.12.006