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Fig. 5: CircGLIS2 overexpressed cancer cell-derived supernatant induces NF-κB signaling and motility activation. | Cell Death & Disease

Fig. 5: CircGLIS2 overexpressed cancer cell-derived supernatant induces NF-κB signaling and motility activation.

From: Circular RNA GLIS2 promotes colorectal cancer cell motility via activation of the NF-κB pathway

Fig. 5: CircGLIS2 overexpressed cancer cell-derived supernatant induces NF-κB signaling and motility activation.

a Luciferase report assay of pGL4.32 transfected 293 T cells co-culture with the supernatant of DLD1 cells (cVC or cGLIS2). b Western blot assay showing the p-p65 and p65 levels in DLD1 cells co-culture with the supernatant of circGLIS2 overexpression (cG) or vector control (cVC) CRC cells. c Schematic protocol for the supernatant associated transwell assay. d Transwell assay showing the migration ability of DLD1 that were co-culture with the supernatant of circGLIS2 overexpression or vector control CRC cells. Scale bars: 250 μm. Bars represent the number of migrated cells. Data are represented as mean ± SD. **P < 0.01.

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