Skip to main content
Springer Nature Link
Account
Fig. 2: Bclx-vMO regulated Bcl-x splicing mode in GBM cells effectively. | Cell Death & Disease

Fig. 2: Bclx-vMO regulated Bcl-x splicing mode in GBM cells effectively.

From: Modification of BCLX pre-mRNA splicing has antitumor efficacy alone or in combination with radiotherapy in human glioblastoma cells

Fig. 2: Bclx-vMO regulated Bcl-x splicing mode in GBM cells effectively.

A Alternative splicing mode and splicing regulation by SSOs in cancer cells. B Localization and delivery efficiencies of Bclx-vMO using Laser confocal microscopy. Fluorescence signal represented the Bclx-vMO delivered by Endo-Porter combined to the target RNA and increased with the amount of Bclx-vMO used. C–F A172 cells were transfected with 4, 8, μM vMO. The expression of Bcl-xL and Bcl-xS were analyzed by PCR and western blot. Bclx-vMO elevated Bcl-xS at the expense of Bcl- xL both at mRNA (C, D) and protein levels (E, F), and significantly decreased the ratio of Bcl-xL/Bcl-xS. G Data are shown as mean values ± S.D. from three independent experiments. One-Way ANOVA followed by Dunnett’s multiple comparisons test are reported. For all panels “**” indicates p < 0.01, “***” indicates p < 0.001, “****” indicates p < 0.0001, “ns” indicates no significance.

Back to article page