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Fig. 5: Blocking BCLX splicing regulation-induced autophagy significantly attenuates apoptosis in A172 cells. | Cell Death & Disease

Fig. 5: Blocking BCLX splicing regulation-induced autophagy significantly attenuates apoptosis in A172 cells.

From: Modification of BCLX pre-mRNA splicing has antitumor efficacy alone or in combination with radiotherapy in human glioblastoma cells

Fig. 5: Blocking BCLX splicing regulation-induced autophagy significantly attenuates apoptosis in A172 cells.

A A172 cells were co-incubated with CQ or 3-MA and Bclx-vMO for 48 h. Cell viability was assessed by MTT assay. B, C A172 cells were co-incubated with CQ or 3-MA and Bclx-vMO for 48 h. Then, the apoptosis rate of A172 cells detected by flow cytometer. Quantification of apoptosis in cells treated is presented. D, E 3D Cell viability of A172 combined-treated with Bclx-vMO and CQ was measured using SYTOX Green. F, G Total proteins of A172 cells were extracted and the expression of proteins related to apoptosis and autophagy was detected by western blot. The concentration of Rs-vMO and Bclx-vMO used was 4 μM when not specified. Data are shown as mean values ± S.D. from ≥three independent experiments. One-Way ANOVA followed by Dunnett’s multiple comparisons test are reported. For all panels “*” indicates p < 0.05, “**” indicates p < 0.01, “***” indicates p < 0.001, “****” indicates p < 0.0001, “ns” indicates no significance.

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