Abstract
Dynein complexes are large, multi-unit assemblies involved in many biological processes via their critical roles in protein transport and axoneme motility. Using next-generation sequencing of infertile men presenting with low or no sperm in their ejaculates, we identified damaging variants in the dynein-related gene AXDND1. We thus hypothesised that AXDND1 is a critical regulator of male fertility. To test this hypothesis, we produced a knockout mouse model. Axdnd1−/− males were sterile at all ages but presented with an evolving testis phenotype wherein they could undergo one round of histologically replete spermatogenesis followed by a rapid depletion of the seminiferous epithelium. Marker experiments identified a role for AXDND1 in maintaining the balance between differentiation-committed and self-renewing spermatogonial populations, resulting in disproportionate production of differentiating cells in the absence of AXDND1 and increased sperm production during initial spermatogenic waves. Moreover, long-term spermatogonial maintenance in the Axdnd1 knockout was compromised, ultimately leading to catastrophic germ cell loss, destruction of blood–testis barrier integrity and immune cell infiltration. In addition, sperm produced during the first wave of spermatogenesis were immotile due to abnormal axoneme structure, including the presence of ectopic vesicles and abnormalities in outer dense fibres and microtubule doublet structures. Sperm output was additionally compromised by a severe spermiation defect and abnormal sperm individualisation. Collectively these data identify AXDND1 as an atypical dynein complex-related protein with a role in protein/vesicle transport of relevance to spermatogonial function and sperm tail formation in mice and humans. This study underscores the importance of studying the consequences of gene loss-of-function on both the establishment and maintenance of male fertility.
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Introduction
Spermatogenesis is the process wherein sperm are produced and involves three broad processes: (1) stem cell (spermatogonia) renewal and proliferation via mitosis to produce spermatocytes, (2) the process of meiosis in spermatocytes where chromosome number is halved, and (3) the complete transformation of round haploid germ cells into spermatozoa with the potential for motility via processes collectively known as spermiogenesis. All aspects of spermatogenesis are dependent on microtubule-mediated protein and organelle transport [1, 2]. This is particularly evident during the process of spermiogenesis wherein the sperm assembly is achieved [3]. During spermiogenesis multiple related transport pathways exist, including along cytoplasmic microtubules through classical protein transport pathways, the bi-directional transport of proteins along the microtubules of the manchette via a process called intra-manchette transport (IMT) and along the microtubules of the axoneme via the classical, in addition to likely modified versions of the intra-flagellar transport (IFT) pathway [4, 5]. A family of complexes essential for many of these processes is the dyneins [6, 7]. There are two types of dynein complexes, the cytoplasmic dyneins, which transport cellular cargoes along microtubules towards their minus end within the cell cytoplasm [8,9,10,11], and axonemal dyneins, which hydrolyse ATP to drive the beating of all motile cilia, including the sperm tail [12,13,14].
The canonical cytoplasmic dynein complex is composed of a dimer of two dynein heavy chains to which two intermediate chain, two light intermediate chain and three light chain dynein subunits bind [8, 15]. In contrast to cytoplasmic dyneins, axonemal dynein complexes are made up of three heavy chain subunits (α-, β- and γ-), alongside a unique set of light chain subunits not previously associated with the cytoplasmic dynein complex [16]. In both classes of dynein complexes, the heavy chain dynein components act as the motor/engine of the complex. The heavy chains each possess a microtubule-binding domain adjacent to an AAA module [16, 17], which facilitates ATP hydrolysis to power complex movement along microtubules or cell motility. Following assembly of the core dynein complex, other regulatory proteins are recruited to enhance activity and specify which cargoes (passengers [cytoplasmic]) or adaptors can bind to each complex sub-type [18, 19]. Different adaptor (auxiliary) protein combinations exist within different tissues and are likely involved in different biological processes [20]. The full suite set of functions dynein complexes take part in spermatogenesis are yet to be fully elucidated [4].
Previously we used whole exome sequencing on a group of infertile men with azoospermia (no sperm in their ejaculate) and identified a novel biallelic stop-gain variant in the dynein-related gene AXDND1 [21]. While AXDND1 contains a dynein light chain domain and is thus annotated as an axonemal dynein light chain-containing protein, it is notably different from the typical light chain dyneins. Specifically, at 120-125 kDa, AXDND1 is considerably larger than bona fide mammalian dynein light chain proteins (~8–30 kDa), and does not contain other domains typical of light chain dyneins [9]. AXDND1 does however contain a conserved PF10211 domain, homologous to the core dynein domain in DNALI1 and Chlamydomonas p28 [9, 22]. While untested, we predict this domain likely confers AXDND1 the ability to interact with dynein heavy chains, the large motor protein subunits that drive cell motility or protein transport complex motility along microtubules [8]. Previously it has been shown that the loss of Axdnd1 function in mice results in male infertility due to its role in sperm head shaping via the manchette and tail assembly during spermiogenesis [23, 24]. This phenotype was, however, a poor match with the azoospermic clinical presentation of the infertile man we identified to be carrying a stop-gain variant.
To further test the role of AXDND1 in male fertility, we generated an Axdnd1 knockout mouse line and rigorously assessed the effects of AXDND1 loss on germ cell development and male fertility. We aimed to test if AXDND1 was a human male infertility gene and validate its link to azoospermia in the infertile men containing AXDND1 variants. We also aimed to investigate the spermatogenic processes AXDND1 is involved in, using histological and sperm functional assays. Specifically, we investigated the role of AXDND1 in germ cell commitment to spermatogenesis during early postnatal development (days 3–7) and the role of AXDND1 in testis germ cell maintenance, spermatid remodelling and sperm function over a range of 40–180 days of age.
Materials and methods
Mouse model production
The structure for human AXDND1 and mouse Axdnd1 transcript information (ENSG00000162779 and ENSMUSG00000026601, respectively) are shown in Supplementary Fig. 1 and data was sourced from Ensembl. To test the requirement for AXDND1 in male fertility, Axdnd1 knockout (Axdnd1−/−) mice were generated by the Monash Genome Modification Platform (MGMP; Monash University, Australia) using CRISPR/Cas9 technology on C57BL/6J mice. Excision of exon 5 (ENSMUSE00001068050) of the longest Axdnd1 transcript ENSMUST00000213088.1 (Supplementary Fig. 1; transcript 211) was achieved using CRISPR guide sequences shown in Supplementary Table 1 and led to a premature stop codon in exon 6. The deletion was confirmed and characterised by Sanger sequencing. This approach generated two founder lines: one with a 564 bp deletion and 5 bp insertion (Axdnd1del1 line) and another with a 571 bp deletion (Axdnd1del2 line) – both contained a premature stop codon in exon 6. After mating founder mice to wild-type C57BL/6J mice, resulting mutant Axdnd1+/− mice were used to generate Axdnd1−/− individuals. Axdnd1 expression levels for the longest transcript (Axdnd1-211) and a smaller transcript (Axdnd1-208) that does not contain exon 5 (and thus was not targeted by the CRISPR deletion) were assessed in testis samples via qPCR as previously described [25], using primers shown in Supplementary Table 1.
Analysis of Axdnd1 expression
Whole organ RNA was extracted from adult mouse brain, epididymis, heart, liver, lung, spleen and testis to investigate Axdnd1 expression across tissues. RNA was also extracted from the testes of mice aged 0–50 days to investigate Axdnd1 expression throughout the first wave of spermatogenesis. Primers used to detect Axdnd1 and housekeeping gene Ppia are shown in Supplementary Table 1. As a direct measure of AXDND1 and Axdnd1 germ cell expression, we also utilised single-cell RNA sequencing data generated through previous studies in our lab [26, 27].
Fertility analysis of Axdnd1 −/− males
Fertility analyses were performed on males from the Axdnd1del1 line over an age range of 30–180 days. Phenotyping was also performed on adult males from the Axdnd1del2 line at 70–90 days of age. The fertility and reproductive parameters of wildtype and Axdnd1−/− males were assessed using the pipeline outlined in [28]. In brief, at least four mice of each genotype were assessed for fertility at two age points (42–50 and 70–80 days) by mating with two young wild-type females each. These ages equate to just after the first wave of spermatogenesis and epididymal maturation has completed, and mature adult males, respectively. Animals were not allocated or randomised into groups – rather they were assessed based on genotype. Sample size was estimated based on severity of the phenotype and experience in each assay. Investigators were blinded to genotype prior to analyses.
Sperm motility was assessed via computer-assisted sperm analysis as described previously [29], including in an additional experiment where 1 mM of membrane permeable ATP (ATP disodium salt hydrate [A2383 – Merck, Australia]) was added in an attempt to rescue motility. Remaining sperm (and immature germ cells) were washed in 1 × PBS then fixed in 4% paraformaldehyde for 10 min and resuspended in PBS. Cells were then settled on SuperFrost+ slides overnight at 4 °C for further analysis. In additional experiments, epididymides were collected for calculation of epididymal sperm content [30], and testes and epididymides were collected for and fixed for transmission electron microscopy analysis as detailed below.
Histological assessment of reproductive tissues and sperm/epididymal luminal cell content
Testis sections were stained with periodic acid-Schiff’s and haematoxylin reagents (PAS), while epididymis sections were stained with haematoxylin and eosin. Sperm and other cells settled to SuperFrost+ slides were stained with haematoxylin and eosin. All slides mounted in DPX under a 1.0 × coverslip for light microscope analysis using an Olympus BX-53 microscope fitted with an Olympus 392 DP80 camera.
To quantify the age-related changes in testis histology, the incidence Sertoli cell-only tubules, severe hypospermatogenesis and vacuoles, were counted at 40, 70 and 180 days of age, n = 3 mice/age/genotype. Sertoli cell-only was assigned when histology showed no germ cells, while severe hypospermatogenesis (approaching Sertoli cell-only) was recorded when very few germ cells were present. Intact tubules contained no defects in each of the three categories. Round spermatid numbers were counted at postnatal day 22 in a minimum of 10 stage V equivalent tubule cross-sections (round and oval tubules) per animal to investigate germ cell commitment to spermatogenesis (n = 5/genotype).
To quantify apoptosis in the testis of 40-, 70-, and 180-day-old males, testis sections were co-stained with 0.5 μg/ml cleaved caspase 3 (Cell Signalling Technology, Australia, Ab 9664) and 0.5 μg/ml cleaved caspase 7 (Cell Signalling Technology, Australia, Ab 9491) antibodies. Caspase-positive cells were counted in at least 50 tubules chosen randomly per male (n > 3 mice/genotype/age).
To investigate immune cell infiltration, testes were immersed in Tissue-Tek O.C.T. reagent (Sakura Finetek, CA, USA) prior to freezing in an ethanol dry ice slurry. Frozen testes were cryo-sectioned onto SuperFrost+ slides then stained with CD45 (BD Pharmingen [BD Biosciences] 550539) and 0.5 µg/ml smooth muscle actin primary antibody (Sigma A2547) antibodies overnight at 4 °C, after which they were stained with appropriate secondary antibodies, followed by 10 µg/ml DAPI (Thermo Fisher Scientific).
AXDND1 localisation was investigated in germ cells and sperm via tubule squash preparations as per [31]. Fixed germ cells were incubated in 6 µg/ml AXDND1 antibody (Sigma HPA071114) overnight at 4 °C and stained with appropriate secondary antibodies, 2 µg/ml wheat germ agglutinin-Alexa Fluor 555 (acrosome marker; Thermo Fisher Scientific W32464) and 10 µg/ml DAPI. Localisation of AXDND1 via immunohistochemistry and the use of western blotting on whole testis lysate failed to produce specific staining or bands.
Investigation of the blood–testis barrier integrity and immune infiltration
A biotin tracer (EZ-Link™ NHS-Biotin; Thermo Fisher Scientific, Australia) was used to test the integrity of the blood–testis barrier in Axdnd1−/− mice as described previously [32]. Sections were stained with 0.5 µg/ml smooth muscle actin primary antibody (Sigma A2547) overnight at 4 °C, followed 1 µg/ml donkey anti-mouse secondary and 2 µg/ml streptavidin-Alexafluor-488 reagent (to visualise biotin, Thermo Fisher Scientific). Nuclei were counterstained with 10 µg/ml DAPI (Thermo Fisher Scientific) and slides mounted with Dako fluorescence mounting medium (Agilent).
Investigation of spermatogonia dynamics during the first wave of spermatogenesis
To quantify spermatogonia numbers, including those committing to spermatogenesis, during the first wave of spermatogenesis, we investigated germ cell content in testis sections at postnatal days 3–7 and 22–35. Testis sections were stained with the germ cell marker MVH (day 3–7; 3 µg/ml, Abcam ab13840), the undifferentiated spermatogonia markers PLZF (day 5–35; 0.6 µg/ml, R&D Systems AF2944) and FOXO1 (day 7; 1 µg/ml, Cell Signaling Technology #2880), and differentiating spermatogonia marker c-KIT (day 7; 0.6 µg/ml, R&D Systems AF2944) overnight at 4 °C. Sections were co-stained with 0.5 µg/ml smooth muscle actin antibody (Sigma A2547) and incubated with relevant secondary antibodies, then 10 µg/ml DAPI to stain DNA. The number of tubules with positive staining for MVH or PLZF, as well as the germ cells positive for each marker (MVH, PLZF, FOXO1 and c-KIT), in at least 20 round tubules was counted (n ≥ 3 animals/genotype). Round spermatid numbers were also counted in at least ten tubules of PAS-stained sections at postnatal day 22 (n = 5 animals/genotype).
Electron microscopy
To investigate germ cell and sperm ultrastructure, testes were fixed, processed and imaged as per [30] To view the sperm tail ultrastructure, sperm were isolated from the cauda epididymis and incubated in 100 µl of 1 × PBS for 30 min to strip the plasma membrane as previously described [33].
Statistical analysis
Statistical analyses to determine significance between wild-type and Axdnd1−/− parameters at each age point were performed in Prism (GraphPad, San Diego, USA) using an unpaired t-test with α = 0.05. Where data were not normally distributed, a non-parametric test was used (Mann-Whitney U). For age series data, a t-test was used to compare genotypes at each age point. Significance of apoptosis data was assessed as previously described [34].
Study approval
Patient description
As described previously [21], we used exome sequencing on infertile men presenting with azoospermia or severe oligozoospermia to investigate novel genetic causes of male infertility. Herein, we identified additional variants in AXDND1. Variants in patients 2 (M1557) and 3 (M2628) were identified in the Male Reproductive Genomics (MERGE) cohort including 2412 participants (n = 2127 men with severe/extreme oligo-, crypto- or azoospermia) according to [35]. Testicular biopsies for histological examination are described in [36]. Experimental ethics for patients 2 and 3 were approved by the Ethics Committee of the Ärztekammer Westfalen-Lippe and the Medical Faculty Münster (ethics number 2010-578-f). Informed consent was obtained from all subjects.
Animal ethics
Experimental procedures involving mice followed animal ethics guidelines generated by the Australian National Health and Medical Research Council (NHMRC). All animal experiments were approved by the Animal Experimentation Ethics Committee (BSCI/2017/31) at Monash University, Clayton or The University of Melbourne Animal Ethics Committee (application 20640).
Results
A loss of function variant in AXDND1 is associated with azoospermia and human male infertility
As part of the GEMINI study to define genetic causes of male infertility, we undertook exome sequencing of infertile men presenting with azoospermia or severe oligozoospermia and identified an azoospermic Portuguese man (patient 1) carrying a homozygous stop-gain variant in the dynein gene AXDND1 (c.937T>C; p.R313X) (Fig. 1A) (first reported in [21]). This variant affected exon 10 of 26 in AXDND1, a region that encodes a predicted axonemal dynein light chain (PF10211) domain. As identified by the variant effect predictor in gnomAD, the p.R313X variant is high confidence for a loss of AXDND1 function. The CADD score for this variant is 42, which places it in the top 0.01% of deleterious variants in the genome. The predicted effect of this stop-gain mutation is nonsense-mediated decay of all the full-length RNA transcripts (AXDND1-201, 202 and 214; Supplementary Fig. 1), resulting in a loss of AXDND1 expression, i.e., it is equivalent to a full loss of function mutant in a mouse.
A comparison of the AXDND1/Axdnd1 protein-coding transcripts between human and mouse (Supplementary Fig. 1) revealed that three transcripts in human (AXDND1-201, -202 and -214) and four in mouse (Axdnd1-202, -204, -208 and -211) contained the PF10211 axonemal dynein light chain domain (exons 7–11).
AXDND1 is enriched in haploid male germ cells
We investigated AXDND1 expression using published testis single cell sequencing data [26, 27]. AXDND1/Axdnd1 was expressed in all germ cell types, including spermatogonia, but most highly expressed within late spermatocytes and round spermatids in both men and mice (Fig. 2A, B). Assessment of AXDND1 expression across several human tissues revealed a clear enrichment in testes (Fig. 2C; p < 0.0001).
AXDND1 is essential for male germline maintenance and fertility in mice
To test the role of AXDND1 in male fertility, we generated a knockout mouse model to recapitulate the predicted complete loss of function of the p.R313X AXDND1 variant identified in infertile patient 1 (Table 1). We used CRISPR to remove exon 3 of the principal Axdnd1 transcript and introduce a premature stop codon in exon 4 (Fig. 1B, C). Two founder mouse lines were generated. Following the confirmation of comparable phenotypes in both lines, our analyses focused on the Axdnd1del1 line (described in the methods). Loss of expression of the full-length Axdnd1 transcript in this knockout line was confirmed via qPCR (Fig. 1D), while the expression of a shorter transcript, encoded by exons 7–17 (Axdnd1 transcript 208, Supplementary Fig. 1) was upregulated approximately tenfold (p < 0.05) compared to expression in wild-type testes. Transcript 208 encodes a truncated protein of 55 kDa (compared with 126 kDa for the full-length protein) that contains the predicted PF10211 domain.
Initially, fertility of Axdnd1−/− males was tested at two ages: (1) at the completion of the first wave of spermatogenesis and epididymal maturation (42-50 days of age) and (2) after several rounds of spermatogenesis (70–80 days of age). At both ages, Axdnd1−/− males were sterile (Fig. 3A; p < 0.0001) although mating appeared normal as evidenced by the presence of copulatory plugs (0 pups/plug). Wild-type males plugged and sired several litters (average of 8.25 ± 0.5 and 7.36 ± 1.1 pups/plug, at postnatal days 42-50 and 70-80 respectively) at both ages. No differences were found in the body weight of Axdnd1−/− males compared to wild-type across all ages investigated (Fig. 3B). Equally, seminal vesicle weights were unchanged between genotypes as a proxy for androgen levels (Fig. S2F).
Next, reproductive parameters of wild-type and Axdnd1−/− males were assessed over an age range of 40-180 days (Fig. 3) to explore the role of AXDND1 in establishing the first wave of spermatogenesis and in the maintenance of spermatogenesis. Testis weights were comparable between wild-type and Axdnd1−/− males until 40 days of age, after which a significant divergence was observed (Fig. 3C; p < 0.0001). Axdnd1−/− testes weights progressively declined until 70 days of age, then remained constant through to 180 days of age. At postnatal day 180, Axdnd1−/− testis weight was 45% of that measured from wild-type males. In accordance, epididymis weights were comparable between genotypes up until day 50 of age, then diverged from day 60 of age (Fig. 3D; p < 0.01). Such changes were suggestive of the successful completion of one round of spermatogenesis followed by age-related degeneration. To explore this possibility quantitatively, sperm production was measured as a function of age (Fig. 3E). During the first wave of spermatogenesis (30 and 35 days of age), knockout males generated significantly more sperm compared to wild type (p < 0.01). At 40 days of age, knockout males produced 30% more sperm than age-matched wild-type males (p < 0.0001). By 50 days of age, however, Axdnd1−/− daily sperm production, decreased to 19% of wild-type levels (p < 0.0001). Similar levels of sperm production were maintained through to 180 days of age. Despite no initial increase in sperm number, an analogous pattern of reduced sperm number was observed within the epididymis as a function of age (Fig. 3F). At day 40 of age, sperm counts in Axdnd1−/− epididymides were 12% of wild-type levels (p < 0.0001). Epididymal sperm counts then declined with age, and at 180 days of age were only 0.33% of wild-type levels. The disconnect in daily sperm production in the testis and epididymal sperm at day 40 was suggestive of a widespread spermiation defect. In support of this hypothesis, significant numbers of retained sperm were observed within stage IX–X tubules of day 40 Axdnd1−/− testes (Supplementary Fig. 2B).
A comparable phenotype was observed in the second Axdnd1−/− line (Axdnd1del2, Supplementary Fig. 3), thus confirming that the phenotype was specific to the loss of AXDND1 function and not an undetected ‘off-target’ CRISPR-mediated loss of gene function somewhere else in the genome.
To explore the consequences of AXDND1 loss at a cellular level, we investigated testis histology (Fig. 4) in wild-type and Axdnd1−/− males at 40 (immediately following the completion of the first wave of spermatogenesis), 70 (after several waves) and 180 (aged males) days of age. Testis histology was consistent in wild-type mice regardless of age Fig. 4). Analogous to the situation in wild-type mice (Fig. 4A), all adult germ cell types were present at 40 days of age in Axdnd1−/− testes (Fig. 4B). By 70 days of age, however, and consistent with the changes in testis weight, spermatogenesis was overtly compromised as highlighted by a significant reduction in, or complete absence of, germ cells in a portion of seminiferous tubules, resulting in vacuoles in the seminiferous epithelium (Fig. 4D, red arrowheads). Associated with this was an influx of cells to the interstitial compartment of the testis (Fig. 4D, red dashed lines), which were confirmed to be immune cells by staining for CD45 (Supplementary Fig. 2D). These disruptions to spermatogenesis in knockout testes worsened by 180 days of age (Fig. 4F).
To quantify these changes in spermatogenic quality, the proportion of tubules presenting with complete spermatogenesis, hypospermatogenesis, or a Sertoli cell-only epithelium was quantified (Fig. 4G–J). These data revealed that the proportion of normal tubules with intact spermatogenesis decreased between 40 and 70 days of age in Axdnd1−/− testes (Fig. 4G, p < 0.0001) and accordingly the proportion of tubules with vacuoles and tubules approaching Sertoli cell-only (severe hypospermatogenesis) in Axdnd1−/− testes were both significantly elevated from 70 days of age (Fig. 4H, I; p < 0.0001). As a measure of complete germ cell loss, the number of complete Sertoli cell-only tubules was significantly elevated at 180 days of age (Fig. 4J, p < 0.0001). In agreement with this reduction in germ cell content, the quantitation of germ cell apoptosis via caspase staining revealed a significant increase in apoptotic germ cells in Axdnd1−/− testes at 70 days of age compared to wild type (p = 0.02; Supplementary Fig. 2E).
As described in more detail below, those sperm present within the epididymis were immotile, thus explaining the origin of infertility in young adult males (Fig. 6). In addition to the severe tail defects, the loss of AXDND1 resulted in a significant increase in head shape abnormality (Supplementary Fig. 4). Objective sperm head shaping analysis revealed a reduction in the percentage of sperm heads classified as most normal (cluster 1, p = 0.0033) and an increase in the abnormal clustering (cluster 3, p = 0.023). This finding is in support of a critical role of AXDND1 in manchette dynamics [23, 24] and its localisation to the manchette.
The blood–testis barrier is compromised in Axdnd1−/− testes
The accumulation of immune cells within the interstitial space in older Axdnd1−/− (Fig. 4D, F) males suggested the loss of integrity of the blood–testis barrier. The blood–testis barrier is a series of intercellular junctions between adjacent Sertoli cells that function to protect immunologically foreign meiotic and haploid germ cells from immune system attack [37]. To directly test the function of the blood-testis barrier we incubated testes with a biotin tracer (Fig. 4K–R). This approach revealed that the blood-testis barrier was intact in Axdnd1−/− testes at 40 days of age as evidenced by the localisation of biotin exclusively within the interstitium and the basal compartment of the seminiferous epithelium which is external to the blood–testis barrier. By contrast, in 70- and 180-day-old Axdnd1−/− testes the biotin tracer permeated deep within the tubules (Fig. 4N, P, R). As expected, the blood–testis barrier was intact in wild-type testes at all ages (Fig. 4M, O, Q). These data reveal that the loss of AXDND1 leads to a loss of blood–testis barrier integrity. It is unclear if this is a primary or secondary consequence of AXDND1 loss. Regardless, the loss of blood–testis barrier function would be expected to accelerate the seminiferous tubule degeneration with increasing age.
AXDND1 is required for normal sperm tail development and function
The presence of sperm within day 40 Axdnd1−/− testes and epididymides suggested the possibility of fertility in young animals. However, as described above, the mating experiments revealed males were sterile. To define the origin of this infertility, epididymis histology was examined at 40 days of age (Fig. 5). As outlined above, while sperm were present in the epididymis, numbers were decreased compared to age-matched wild-type males. By 70 days of age (Fig. 5D, H) very few sperm were seen and, instead, the cauda epididymis contained many round cells – likely prematurely sloughed germ cells as confirmed previously [24] and likely immune cells (Supplementary Fig. 2H). This finding was consistent in aged males at 180 days of age, where widespread phagocytosis of spermatid nuclei by immune cells was evident (Fig. 5F).
Of those sperm released by Axdnd1−/− testes at day 40, the majority were abnormal (Fig. 5B, arrows), as highlighted by highly coiled sperm tails (Fig. 6A, E). Most possessed an abnormal midpiece region, abnormal head shaping and/or sperm that were not individualised correctly. Specifically, throughout most of spermatogenesis sister germ cells develop in a syncytium, wherein individual germ cells are separated by intracellular bridges [38]. During the final steps of spermatogenesis, residual cytoplasm is removed, and sperm are ‘individualised’ through largely unknown processes, but insights from flies reveal this process requires functioning actin and microtubule networks [39]. Sperm isolated from the cauda epididymis of Axdnd1−/− males were completely immotile (Fig. 6B, p < 0.0001). Further, motility was not recovered with the addition of membrane-permeable ATP as an exogenous energy source, suggesting a core structural defect within the axoneme [4]. An analysis of midpiece/mitochondrial morphology revealed that the mitochondrial sheath of sperm was poorly formed or missing in 87% of sperm from Axdnd1−/− males but only 5% of wild-type sperm (p < 0.0001; Fig. 6C). Sperm tail length was measured in individual sperm and revealed no significant differences between genotypes, noting this was not possible for those with coiled tails (Fig. 6D).
A more detailed examination of cell ultrastructure via scanning electron microscopy on membrane-stripped sperm revealed that sperm generated by Axdnd1−/− males had highly abnormal mitochondrial sheath structure and mitochondrial morphology (Fig. 6E). In contrast to the neatly coiled mitochondria of sperm from wild-type mice, those from Axdnd1−/− males, possessed poorly coiled mitochondria and gaps within the sheath where no mitochondria were present. Further, in the midpiece compartment of sperm from Axdnd1−/− males, microtubules and outer dense fibres splayed outward from the axoneme core and in some cases appeared to have snapped off. By contrast, the structure of the fibrous sheath of these sperm appeared to be largely normal as observed externally at a scanning electron microscopy level, but also internally as investigated by transmission electron micrographs (Fig. 7E, H, I).
To define why sperm from Axdnd1−/− males were immotile, we used transmission electron microscopy on developing spermatids (testis) and released sperm (epididymal sperm) (Fig. 7). An investigation of step 15–16 spermatids, just before sperm release, and epididymal sperm from Axdnd1−/− males revealed a battery of axoneme and other sperm tail defects (Fig. 7D–F) compared to those from developmentally matched cells from wild-type males (Fig. 7A–C). In most sperm, a portion of axoneme microtubule doublets were missing or ectopically positioned in both the midpiece and the principal piece. In distal principal piece sections, core axoneme ultrastructure, however, appeared broadly normal in sperm from Axdnd1−/− males (Fig. 7F), suggesting defects were exacerbated during the loading of accessory structures. In addition, abnormal ODF loading was observed in virtually all cross-sections (Fig. 7D, E, G–J), as highlighted by absent or flimsy ODF structures. Further, we observed the over-accumulation of granulated bodies (precursor ODFs) in the cytoplasm (Fig. 7K inset – yellow dashed line; Fig. 7L). Ectopic vesicles were also identified in the axoneme (Fig. 7G, asterisks) and as outlined above, mitochondria loading in the midpiece was abnormal (Fig. 7D, G). All these defects are consistent with abnormal transport of proteins and organelles along microtubules – key functions of dynein complexes. Aligning with the finding of failed sperm individualisation, we detected many examples of multiple sperm sharing a common plasma membrane in samples from the epididymis (Fig. 7L).
To further explore if AXDND1 might play a direct role in organelle/protein transport within the sperm tail, we stained spermatids from wild-type and knockout males with an AXDND1 antibody. While the antibodies we tested did not result in specific staining in formaldehyde-fixed testis sections they did appear to be specific in elongating spermatids harvested using the dry-down method and fixed with paraformaldehyde [31]. As shown in Fig. 6F, AXDND1 localised strongly to the midpiece region of sperm and in a punctate manner in the principal piece in developing wild-type spermatids but not in knockout spermatids. AXDND1 also localised to the microtubules of the manchette and the developing head-tail coupling apparatus of spermatids (Supplementary Fig. 2F).
AXDND1 is a regulator of spermatogonial commitment to spermatogenesis
The elevated daily sperm production measured in Axdnd1−/− testes at days 30, 35 and 40 of age followed by a rapid decline is suggestive of AXDND1 playing a role in maintaining the overall balance between spermatogonial self-renewal and commitment to spermatogenesis. To explore this, we quantified the number of round spermatids at day 22 of age (Fig. 8A), where spermatids first appear at postnatal day 20 in wild-type males [40]. This analysis revealed a significant increase in round spermatid number in seminiferous tubules in Axdnd1−/− testes compared to those from wild-type animals (Fig. 8B, p = 0.0026) and, accordingly, a significant increase in the average tubule area (Fig. 8C, p = 0.0071).
To define the origins of the increase in cell number, we quantified the numbers of MVH+ germ cells (pan germ cell marker) in testis sections at postnatal day 3–7 and PLZF+ cells (undifferentiated and early differentiating spermatogonia) from postnatal day 5, when nascent undifferentiated and differentiating spermatogonia have been generated from fetal germ cell precursors (known as gonocytes or pro-spermatogonia) [41], through to day 35 of age. Differentiating spermatogonia directly generated from gonocytes produce the first wave of spermatogenesis while subsequent sperm production is dependent on the undifferentiated pool that contains spermatogonial stem cells (SSCs). Importantly, the proportion of tubules containing MVH+ germ cells was unaffected in Axdnd1−/− testes compared to wild-type controls during the first postnatal week, indicating that germline development occurred normally (Fig. 8D). Specifically, normal numbers of gonocytes populated the testis at postnatal day 3 in both genotypes, suggesting gonocyte proliferation was undisturbed. In wild-type testes, the number of MVH+ germ cells per tubule cross-section increased from postnatal day 3–7 as the nascent spermatogonial pool was established and started to divide (Fig. 8E). However, while the number of MVH+ cells per tubule cross-section in Axdnd1−/− testes was unaffected at postnatal days 3 and 5 versus controls, it was significantly increased at day 7 (Fig. 8E, p < 0.0001), consistent with increased mitotic expansion and/or reduced apoptosis of germ cells [42, 43]. In contrast, the percentage of tubules containing PLZF+ spermatogonia and the number of PLZF+ cells per tubule cross-section were both comparable to controls at all ages assessed (Fig. 8F, G), suggesting that formation and initial maintenance of undifferentiated spermatogonia were unaffected by Axdnd1 knockout.
To extend our analysis, we investigated staining for (1) c-KIT, a marker of differentiated spermatogonia, and (2) the transcription factor FOXO1, an independent marker of undifferentiated spermatogonia that is also expressed in gonocytes [44]. At postnatal day 7, numbers of c-KIT+ spermatogonia per tubule were significantly increased in Axdnd1−/− testes compared to controls (Fig. 8H, p = 0.014), while the abundance of FOXO1+ cells was not significantly changed (Fig. 8I, p = 0.13). These data suggest that the observed increase in germ cells during postnatal testis development was due to increased numbers of differentiating rather than undifferentiated spermatogonia, consistent with normal numbers of PLZF+ spermatogonia in knockout testes (Fig. 8E–G). Importantly, subcellular localisation of FOXO1 is controlled dynamically during spermatogonial development and differentiation. FOXO1 is predominantly cytosolic in neonatal gonocytes, then translocates to the nucleus in undifferentiated spermatogonia but becomes cytosolic then downregulated upon spermatogonial differentiation [44, 45]. At postnatal day 7, numbers of c-KIT- undifferentiated spermatogonia with predominantly nuclear FOXO1 were comparable between Axdnd1−/− and controls (Fig. 8J). However, numbers of c-KIT+ spermatogonia with cytoplasmic FOXO1, i.e., spermatogonia at early differentiation stages, were significantly increased in the knockout at this timepoint (Fig. 8J, p < 0.001).
Combined, our data indicate that AXDND1 is not required for development of the foundational population of undifferentiated spermatogonia during postnatal development but that AXDND1 limits differentiation commitment from the precursor pool and/or survival of nascent differentiating spermatogonia [43], leading to increased sperm production during initial spermatogenic waves upon Axdnd1 deletion. At later timepoints, germline integrity in Axdnd1 knockout testis is lost, indicating that long-term maintenance of the undifferentiated population is dependent on AXDND1.
Additional AXDND1 variants in infertile men
In order to search for additional men carrying AXDND1 variants, cohorts of men within the International Male Infertility Genomics Consortium (imigc.org) were assessed. We identified four variants (Table 1): two nonsynonymous SNVs in a Croatian man (patient 2, M1557) with azoospermia and a Sertoli cell-only presentation, and two nonsynonymous SNVs in a Turkish man with azoospermia (patient 3, M2628), where a biopsy revealed less than 50% of seminiferous tubules bearing elongating spermatids (Supplementary Fig. 5). All four of these variants were classified as variants of unknown significance. While the phases could not be determined in both cases (segregation analysis and long read sequencing), the disease presentation is consistent with patient 1 wherein a complete loss of AXDND1 function variant was identified. Collectively these data indicate that, analogous to the situation in the mouse, AXDND1 is required for the maintenance of human spermatogenesis.
Discussion
Herein we reveal that AXDND1 is essential for male fertility for mice and men, functioning in several phases of spermatogenesis. AXDND1 plays a critical role in regulating the overall balance of spermatogonial commitment versus renewal during the first wave of spermatogenesis. AXDND1 is additionally required for the formation of functional sperm tail structures, sperm individualisation and head shaping, and spermiation. These latter functions are consistent with a role in regulating protein/organelle transport along microtubule structures. Exacerbating the consequences of AXDND1 loss, but highlighting the importance of cell-cell communication in spermatogenesis, while mice are still relatively young, the absence of AXDND1 leads to a loss of blood–testis barrier integrity and the infiltration of immune cells into the seminiferous epithelium. The latter will cause further damage to the seminiferous epithelium. While previous studies have identified AXDND1 to be essential for male fertility in mice, they have not identified the evolving phenotype of Axdnd1−/− mice, the deficits in sperm individualisation, and the key role AXDND1 plays in spermatogonia and thus the longer-term integrity of spermatogenesis [23, 24]. Collectively, these data identify AXDND1 as an atypical dynein complex protein that plays multiple roles in mouse and human spermatogenesis.
Importantly, we demonstrated a previously unappreciated role for AXDND1 as a regulator of spermatogonial commitment to spermatogenesis. The increased numbers of differentiating spermatogonia in Axdnd1−/− testes resulted in an elevated sperm number during the first wave of spermatogenesis. However, subsequent waves were disrupted due to progressive germline degeneration. How AXDND1 limits the production of differentiation-committed spermatogonia while ensuring long-term maintenance of undifferentiated states is unclear. This may be explained by it playing a role in the transport of specific cell-regulatory factors. Previous research has shown that the RNA-binding protein DAZL, which is essential for the translation of RNAs important for male germ cell development, interacts directly with the cytoplasmic dynein complex [46]. In addition, it was postulated that RNAs bound to DAZL could be transported by dyneins when required for developmental processes and protein translation [46]. AXDND1 may be involved in a similar process, acting as a dynein adaptor required for specific germ cell-regulatory factors.
AXDND1 was originally annotated as a light chain domain-containing protein of the axonemal dynein class. If this was the case, it would be expected that AXDND1 plays a role specifically in axoneme function to manifest sperm motility. We did observe a complete loss of motility in the absence of AXDND1. We, however, also identified several structural defects in sperm from Axdnd1−/− males which suggest AXDND1 plays a more general role in protein or organelle transport. In support of a function in association with cytoplasmic dynein complexes, AXDND1 localised to the mitochondrial sheath and the neck of sperm, two structures that are involved in cytoplasmic mediated functions. By contrast, AXDND1 was localised only weakly to the principal piece, which forms solely within the ciliary compartment [4], providing only weak evidence that AXDND1 is a core component of the dynein arms of the axoneme.
AXDND1 also contains a PF10211 domain that is conserved in DNALI1, a dynein light intermediate chain which has been shown to interact with cytoplasmic dynein 1 [22]. A variant encoding hypomorphic DNALI1 in mice resulted in axoneme microtubule doublet and fibrous sheath abnormalities [47], suggesting this domain permits interaction with cytoplasmic dynein complexes. These data suggest AXDND1 may act as an adaptor to optimise the interaction between dynein complexes and cargoes (e.g., components of the ODFs) to allow their efficient transport along the microtubules of the manchette to the site of entry of the sperm tail compartment (cytoplasmic dynein 1) and/or via retrograde transport along the developing sperm tail (cytoplasmic dynein 2) in the ciliary compartment. The former interpretation is supported by the presence of abnormal concentrations of granulated bodies in the cytoplasm of elongating spermatids and a failure of mitochondrial loading in elongating spermatids from Axdnd1−/− mice [48]. A role in cytoplasmic dynein complex 2 is supported by the presence of abnormal accumulations of vesicles in the axoneme and as highlighted previously [24]. AXDND1 does not, however, appear to play a role in core intra-flagellar transport that seeds the axoneme core of the sperm tail as evidenced by normal sperm tail lengths and a normal axoneme presentation in the end piece of sperm from the knockout model. Axdnd1 null sperm tails were, however, immotile, and unresponsive to cell-permeable ATP, supporting that the transport and assembly of key components of the dynein arms into the microtubule doublets of the axoneme was compromised.
The loss of sperm individualisation has rarely been seen in mouse models. An additional light chain domain-containing dynein ‘ctp’ that encodes the same PF10211 domain, has, however, previously been shown to be essential for the process of sperm individualisation in flies [36], suggesting a potential conserved role for dyneins in this process. Recently, it was demonstrated that the process of sperm individualisation in mice is driven by an autophagy pathway [49] and likely requires efficient neddylation, a process of targeting cellular material for degradation that is similar to ubiquitination [50]. Whether AXDND1 aids in trafficking members of either pathway to sites for targeted removal of the cytoplasmic bridges and other material required for individualisation should be tested in future studies.
Herein where we describe AXDND1 localises to the manchette, and in confirmation of previous studies, AXDND1 is required for normal manchette structure and thus sperm head shaping [23]. Taken together these data point to a direct role for AXDND1 in generating the tension forces required to shape the sperm nucleus. Of note, the manchette provides dual functions in head shaping and as a scaffold for intra-manchette protein and vesicle transport [4]. As proposed above, data presented here support a role for AXDND1 in the processes of head shaping, protein/vesicle transport along the microtubules of the manchette and ultimately into and along the length of the axoneme and ciliary compartment.
Although the mechanisms of mitochondrial loading into the sperm midpiece are poorly understood, it is hypothesised that the manchette also aids as a scaffold to align the mitochondria parallel to the axoneme prior to their loading onto the ODFs [4]. Dynein is localised to the manchette in early spermatids but its localisation and roles at the time of mitochondrial loading is insufficiently explored [51, 52]. The midpiece presentation in sperm from Axdnd1−/− males is, however, more severe than that previously identified in knockout models wherein the manchette is dysfunctional [30, 53]. The severe mitochondrial sheath defects in Axdnd1−/− males bear resemblance to sperm from models where sperm individualisation is defective [49, 50], raising the possibility it is a secondary effect of failed individualisation. However, given that defects were evident during mitochondrial sheath development in spermatids, and the localisation of AXDND1 to the developing spermatid midpiece, it remains a possibility that AXDND1 plays a direct role in facilitating the loading of mitochondria into the midpiece compartment.
One caveat of this study was the inability to phase the potential compound heterozygous variants in AXDND1 patients 2 and 3. In support of their potential pathogenicity, however, the clinical presentation of both men was azoospermia, which is a match with patient 1 and the Axdnd1 knockout mouse. Patient 3 presented with severe hypospermatogenesis and patient 2 with Sertoli cell only histology. Hypospermatogenesis leading to an eventual Sertoli cell only phenotype was observed in the knockout mouse model, highlighting the benefits of studying the evolving phenotype with age.
In addition to providing significant new data and insights into the role of AXDND1 in sperm tail development in this study [24], we identify that AXDND1 is critically required to sustain the balance between spermatogonial maintenance and commitment to spermatogenesis. The loss of AXDND1 resulted in a progressive loss of germ cell content and a breakdown of the blood–testis barrier that led to immune infiltration. Sperm produced during the first wave were immotile due to abnormal axoneme structure, including the presence of ectopic vesicles, loss of a portion of the outer dense fibres and microtubule doublets, abnormal mitochondrial sheath and sperm head formation, and poor sperm individualisation. Our data shed light on previously unappreciated functions of AXDND1 in male germ cell development and suggest that it functions in protein transport as an atypical component of the cytoplasmic dynein complexes. Finally, we identified an additional four potentially compound heterozygous biallelic variants of unknown significance in AXDND1, in men suffering non-obstructive azoospermia suggesting that the insights into the function of AXDND1 in the mouse apply to humans.
Data availability
Data are available upon request to the corresponding author.
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Acknowledgements
Luisa Meyer for technical support.
Funding
This work was supported by a National Health and Medical Research Council grant awarded to MKOB and DFC (APP1120356), a National Institutes of Health of the United States of America grant (R01HD078641) to DFC and KIA, and was carried out within the framework of and supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) sponsored Clinical Research Unit ‘Male Germ Cells’ (CRU326, project number 329621271 to FT and CF).
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BJH, DJM, JN, and AEOC undertook experiments. BJH, RH, JD, and MKOB analysed the data. BJH, RH, JD and MKOB designed the experiments. BJH, RH and MKOB wrote the paper. The patients were recruited by AL and SK, and their DNA sequenced by LN, CF, FT, KIA and DFC. All authors provided intellectual input and feedback on drafts. All authors reviewed and approved the final publication.
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Houston, B.J., Nguyen, J., Merriner, D.J. et al. AXDND1 is required to balance spermatogonial commitment and for sperm tail formation in mice and humans. Cell Death Dis 15, 499 (2024). https://doi.org/10.1038/s41419-024-06874-5
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DOI: https://doi.org/10.1038/s41419-024-06874-5
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