Fig. 5

Ebselen stabilizes ALS mutant SOD1 in living cells. a Overlay of ¹H-¹⁵N NMR spectra acquired on human cells expressing [U-¹⁵N] labelled SOD1 ALS mutants G93A (a) and A4V (b) in the absence (green/orange) and in the presence (black) of ebselen in the external medium. NMR signals clustered in the region around 8.3 p.p.m. (¹H) are characteristic of unstructured SOD1 species. c Western blot analysis of cell lysates expressing wild-type and mutant SOD1 in the absence and in the presence of ebselen. Original image Supplementary Fig. 11. d Expression levels of wild-type and mutant SOD1 in cell samples treated with ebselen relative to the corresponding untreated samples; error bars represent s.d. (n = 3); * indicates significant differences (ratio t-test, p < 0.05)