Fig. 4: Three-dimensional (3D) thermal imaging in vivo.

a Representation of an anaesthetized nude mouse to which Ag₂S nanothermometers (NTs, 100 μL, 8 mg mL⁻¹) are injected retro-orbitally to obtain a thermal readout of ventral vasculature. b Hyperspectral image (integrated intensity over the 1000–1400 nm range) of the ventral vasculature of the mouse obtained under 808-nm excitation, alongside the binarized image used as a mask to select the pixel to be analyzed are reported. c 3D thermal image of the vasculature obtained from the analysis of the hyperspectral image in (b). The projections of the experimental points along the xy and xz planes are also reported in gray.