Fig. 1: Comparison of embryonic and regenerative transcriptomes.
From: Whole body regeneration deploys a rewired embryonic gene regulatory network logic
A General morphology of N. vectensis during embryonic development and regeneration (black; f-actin/Phalloidin, red; nuclei/DAPI). Dashed line: amputation site, ten tentacles, pha pharynx, mes mesenteries, bco body column. B Principal component analysis (PCA) of three batch-corrected embryonic datasets39,43,44. Plot legend indicates timepoint and dataset (Fischer et al.: Helm, F et al.:(H) and Warner et al.:(W). Most of the variation is observed in the first 24 h of development. C PCA of regeneration dataset sampled at uncut (UC), 0, 2, 4, 8, 12, 16, 20, 24, 36, 48, 60, 72, 96, 120, and 144 hpa. Data were extracted from the NvERTx database39. Regeneration proceeds through a wound-healing phase (0–4 hpa) followed by the early regenerative program (8–20 hpa) and ending with a late regenerative program which approaches the uncut condition (24–144 hpa). D PCA of embryonic versus regeneration samples. Embryogenesis (blue) exhibits far greater transcriptomic variation than regeneration (red). E Comparison of differentially expressed (|log2(FC)| > 2 and FDR < 0.05 for any timepoint comparison against t0, where t0 = 7 hpf for embryogenesis and 0 hpa for regeneration) “dynamic” genes during embryogenesis (blue) and regeneration (green). F Global overview of the regeneration specific genes expression and classification, referring to clusters shown in Fig. S2. G GO term enrichment for dynamic regeneration genes. GO-term enrichment was assessed using a one-sided Fisher’s exact test (P < 0.02).