Figure 6
From: Microbial colonization is required for normal neurobehavioral development in zebrafish
Colonization of the zebrafish GI tract with A. veronii:dTomato is sufficient to block behavioral hyperactivity at 10 dpf. Axenic embryos were colonized with A. veronii:dTomato at 1 dpf and imaged at 2–6 dpf or subjected to locomotor testing at 10 dpf. Axenic embryos were also colonized with V. cholerae:GFP at 1 dpf and tested for locomotor activity at 10 dpf. (A) Experimental design. (B) Representative images of axenic embryos conventionalized on day 1 with A. veronii (AX + A. veronii) at 2–6 dpf. At 10 dpf, distance moved in axenic larvae colonized on day 1 with (C,D) A. veronii or (E,F) V. cholerae (AX + V. cholerae) for each 2 min period or mean distance during the 10 min dark period are shown. Black and white bars represent dark and light periods, respectively. N = 19–36 larvae per group. Different letters indicate significance (p < 0.05). Error represents SEM.