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Table 1 Experimental groups.

From: Microbial colonization is required for normal neurobehavioral development in zebrafish

Group

Abbreviation

Description

Axenic

AX

Microbe-free zebrafish

Conventionally colonized

CC

Normally colonized zebrafish

Conventionalized with live bacteria

ACx

Axenic zebrafish conventionalized on day x with microbes harvested from a zebrafish facility (x = 1, 3, 6, or 9 dpf)

AX + A. veronii

Axenic zebrafish conventionalized on day 1 with A. veronii

AX + V. cholerae

Axenic zebrafish conventionalized on day 1 with V. cholerae

Treated

AX + HKEB

Axenic zebrafish exposed to heat-killed E. coli at 1, 6, 7, 8, and 9 dpf

AX + HKST

Axenic zebrafish exposed to heat-killed S. typhimurium at 1, 6, 7, 8, and 9 dpf

AX + pam3CSK4

Axenic zebrafish exposed to pam3CSK4 at 1, 6, 7, 8, and 9 dpf

AX + Poly:IC

Axenic zebrafish exposed to Poly(I:C) at 1, 6, 7, 8, and 9 dpf