Novel Plasmodium antigens identified via genome-based antibody screen induce protection associated with polyfunctional T cell responses

Schussek, Sophie; Trieu, Angela; Apte, Simon H.; Sidney, John; Sette, Alessandro; Doolan, Denise L.

doi:10.1038/s41598-017-15354-0

Table 4 Spearman correlation analysis.

From: Novel Plasmodium antigens identified via genome-based antibody screen induce protection associated with polyfunctional T cell responses

A	CD8				CD4
IFN-γ	+++	++−	+−+	+−−	+++	++−	+−+	+−−
Spearman r	0.7238	0.3934	0.4200	0.2118	0.4876	0.0816	0.2669	-0.1 059
P value (two-tailed)	0.0007	0.1063	0.0827	0.3988	0.0401	0.7476	0.2844	0.6759
IL-2	+++	++−	−++	−+−	+++	++−	−++	−+−
Spearman r	0.8147	0.3955	0.3913	0.0444	0.8043	0.4471	0.5607	0.0898
P value (two-tailed)	<0.0001	0.1043	0.1083	0.8611	<0.0001	0.0629	0.0155	0.7230
TNF	+++	+−+	−++	−−+	+++	+−+	−++	−−+
Spearman r	0.7128	0.5468	0.6901	0.4142	0.7211	0.5510	0.7727	0.4536
P value (two-tailed)	0.0009	0.0189	0.0015	0.0875	0.0007	0.0178	0.0002	0.0587
B	CD8				CD4
IFN-γ	+++	++−	+−+	+−−	+++	++−	+−+	+−−
Spearman r	0.5625	0.3042	0.3319	0.1947	0.4295	-0.0150	0.2138	-0.1698
P value (two-tailed)	0.0151	0.2197	0.1785	0.4388	0.0753	0.9530	0.3943	0.5006
IL-2	+++	++−	−++	−+−	+++	++−	−++	−+−
Spearman r	0.8125	0.3115	0.2815	0.0005	0.8208	0.3333	0.5439	-0.0418
P value (two-tailed)	<0.0001	0.2083	0.2578	0.9984	<0.0001	0.1765	0.0196	0.8691
TNF	+++	+−+	−++	−−+	+++	+−+	−++	−−+
Spearman r	0.7054	0.5524	0.6362	0.2193	0.7426	0.5673	0.7302	0.3746
P value (two-tailed)	0.0011	0.0174	0.0045	0.3820	0.0004	0.0141	0.0006	0.1257
C	CD8				CD4
IFN-γ	+++	++−	+−+	+−−	+++	++−	+−+	+−−
Spearman r	0.7408	0.5173	0.2930	0.2909	0.5931	0.4242	0.3120	0.2007
P value (two-tailed)	0.0004	0.0279	0.2380	0.2416	0.0095	0.0793	0.2076	0.4245
IL-2	+++	++−	−++	−+−	+++	++−	−++	−+−
Spearman r	0.6091	0.5264	0.4623	0.2307	0.5773	0.5036	0.5284	0.3435
P value (two-tailed)	0.0073	0.0248	0.0534	0.3570	0.0121	0.0331	0.0242	0.1628
TNF	+++	+−+	−++	−−+	+++	+−+	−++	−−+
Spearman r	0.4099	0.1982	0.3333	0.2969	0.4630	0.2868	0.5010	0.2496
P value (two-tailed)	0.0911	0.4304	0.1765	0.2315	0.0530	0.2485	0.0342	0.3178

+++: IFN-γ/IL-2/TNF triple positive; ++−: IFN-γ/IL-2 double positive; +−+: IFN-γ/TNF double positive; −++: IL-2/TNF double positive; +−−: IFN-γ single positive; −+−: IL-2 single positive; −−+: TNF single positive. A: Protective index = sum of reduction of parasite burden in the liver and in the blood for each immunisation calculated as the mean reduction of parasite burden for all protected mice per group times the percentage of protected mice per group (protection ≥25% reduced parasite burden compared to control group) (see Table 3). B: Protective capacity calculated as mean reduction of parasite burden in the liver for all protected mice times the percentage of protected mice/group (protection ≥25% reduced parasite burden compared to control group). C: Protective capacity calculated as mean reduction of parasite burden in the blood for all protected mice times the percentage of protected mice/group (protection ≥25% reduced parasite burden compared to control group).

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