Figure 4

Classifying Sample Curves Using PCR Endpoint. This graph shows the endpoint of every one of our 796 PCR curves across all three color channels, excluding negative detections. The horizontal dashed lines represent the boundaries of the ‘bins’ used to match each curve to a level. For example, any sample with scaled endpoint intensity landing between 2.5 and 3.5 is identified as being in level 3, indicating that it contains targets A and B. The fact that all A + B samples in all color channels landed between these boundaries indicates that all A + B samples were classified correctly.