Figure 4

UVB-induced cell death is independent of oxidation in primary human fibroblasts. Prior to UVB irradiation, fibroblasts were incubated 30 min with different antioxidants. Cells were then irradiated in PBS using a lethal UVB dose (30 kJ/m²). The different antioxidant used were: (a) Broad-spectrum oxidation inhibitor N-acetylcysteine (NAC, 2.5 mM), (b) broad-spectrum oxidation inhibitor α-Tocopherol (α-Toco, 10 μM), (c) triplet energy acceptor Ethyl Sorbate (EthS, 5 μg/μl). Cellular metabolic activity was assessed at different time points post UVB exposition (0, 1, 6, 24 h) using a MTS assay. Irradiated cells were normalised on unirradiated cells of the same condition. None of the antioxidant tested had an effect on UVB-induced cellular metabolic activity loss. N = 4. (d) Table of antioxidants used, their type and solubility.