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Figure 2 | Scientific Reports

Figure 2

From: Identification, heterologous production and bioactivity of lentinulin A and dendrothelin A, two natural variants of backbone N-methylated peptide macrocycle omphalotin A

Figure 2

Production of backbone N-methylated peptide macrocycles in P. pastoris. (A) Methylation pattern of OphMA, OphMA_LedCORE and OphMA_DbiCORE. Methylated residues were determined by LC–MS/MS analysis of the C-terminal tryptic fragments and are shaded in green (Supplementary Fig. S2A–C). (B) Production of omphalotin A. The extracted compound was detected using LC–MS and compared to the chemically synthesized omphalotin A standard (216 ng/ml). In addition to omphalotin A, fully methylated, linear (core) peptides and short linear peptides lacking the C-terminal three residues (VIG) were detected. (C) Production of lentinulin A and (D) dendrothelin A. The detected peptides were confirmed by LC–MS/MS. The confirmed positions of methylation are depicted using filled circles, while methylated residues inferred from MS/MS are shown in open circles. Residues different from omphalotin A are underlined. The mass difference (represented in ppm) between observed values and theoretical mass is indicated in brackets for each compound.

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