Figure 3

Annotated LC–MS/MS spectra of fully methylated, linear core peptides of omphalotin A, lentinulin A and dendrothelin A produced in P. pastoris. The peptide sequence of each peptide is presented together with the corresponding b-ions. The confirmed positions of methylation are depicted using filled circles, while methylated residues inferred from MS are shown in open circles. Residues different from omphalotin A are underlined. Annotated LC–MS/MS spectra of (macrocyclic) omphalotin A, lentinulin A and dendrothelin A are provided in Supplementary Fig. S5.