Identification, heterologous production and bioactivity of lentinulin A and dendrothelin A, two natural variants of backbone N-methylated peptide macrocycle omphalotin A

Table 1 In vitro nematotoxicity of recombinant omphalotin A, lentinulin A and dendrothelin A towards M. incognita.

	Day 1		Day 3		Day 7		Day 14
	LC50	95% CI	LC50	95% CI	LC50	95% CI	LC50	95% CI
Lentinulin A	0.341	0.308	0.121*	<< 0.1	0.093	0.072	0.063	0.047
Lentinulin A	0.341	0.377	0.121*	>> 3.0	0.093	0.121	0.063	0.083
Dendrothelin A	>> 3.0*	6.173	>> 3.0*	<< 0.1	0.472	0.371	0.167	0.120
Dendrothelin A	>> 3.0*	>> 3.0	>> 3.0*	>> 3.0	0.472	0.601	0.167	0.231
Omphalotin A	0.840	0.772	0.245	0.212	0.125	0.094	0.067	0.047
Omphalotin A	0.840	0.914	0.245	0.283	0.125	0.166	0.067	0.095
Fluopyram	0.375	0.337	0.084*	<< 0.1	0.040	0.029	0.060*	<< 0.1
Fluopyram	0.375	0.417	0.084*	>> 3.0	0.040	0.056	0.060*	>> 3.0

The nematotoxicity of the three macrocycles was determined at different concentrations and exposure times on M. incognita second-stage juveniles (J2) as described in Materials and methods. Shown are the estimated lethal concentrations 50% (LC50) in μM and the 95% confidence interval (95% CI) on those estimates. * indicates that the statistical model did not adequately fit the data and thus that the values are less confident (see Supplementary Dataset 1 for raw data and Supplementary Fig. S8 for statistical analysis). The nematicide fluopyram was used as positive control.