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Figure 1 | Scientific Reports

Figure 1

From: 16S rRNA gene primer choice impacts off-target amplification in human gastrointestinal tract biopsies and microbiome profiling

Figure 1

The problem of off-target amplification in samples from esophagus, stomach, and duodenum biopsies. (A) Percentage of amplicon sequence variants (ASVs) aligned to the human genome produced by Illumina MiniSeq 2 × 150 bp sequencing of amplicons targeting the V4 and V1–V2 regions. E esophagus, EAC esophageal adenocarcinoma, S stomach, D duodenum. (B) Alignment of V4 region amplification primers to the Homo sapiens mitochondrion haplogroup; sequence ID corresponds to the NCBI nucleotide database.

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