Fig. 6

LHX2 coordinates variations in chromatin accessibility at the Sox2 locus. Custom tracks of E14 and P2 LHX2 ChIP-Seq reads and age-matched ATAC-Seq from purified RPCs and post mitotic precursors were configured on the mm10 UCSC murine genome assembly. Regions of interest targeted by LHX2, where variations by RNA-Seq and/or ATAC-Seq coverage are observed in Lhx2 cKO retinas are highlighted. Association with H3K27ac-labeled enhancer elements is also indicated. The LHX2-coordinated regulatory module is defined as follows: regulatory regions that display variations in ATAC-Seq coverage without a corresponding variation in the nearest transcript by E14 Lhx2 cKO RNA-Seq are putatively assigned (arrows) to the nearest gene exhibiting variation in transcript levels