Abstract.
Human low-density lipoprotein (LDL) is a highly complex nano-particle built up of various lipid classes and a single large protein moiety (apoB-100) owning essential physiological functions in the human body. Besides its vital role as a supplier of cholesterol and fat for peripheral tissues and cells, it is also a known key player in the formation of atherosclerosis. Due to these important roles in physiology and pathology the elucidation of structural and dynamical details is of great interest. In the current study we drew a broader picture of LDL dynamics using elastic incoherent neutron scattering (EINS) as a function of specified temperature and pressure points. We not only investigated a normolipidemic LDL sample, but also a triglyceride-rich and an oxidized one to mimic pathologic conditions as found under hyperlipidemic conditions or in atherosclerotic plaques, respectively. We could show that pressure has a significant effect on atomic motions in modified forms of LDL, whereas the normolipidemic sample seems to cope much better with high-pressure conditions irrespective of temperature. These findings might be explained by the altered lipid composition, which is either caused through elevated triglyceride content or modifications through lipid peroxidation.
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References
E.J. Benjamin et al., Circulation 135, e146 (2017)
M. Krieger, Proc. Natl. Acad. Sci. U.S.A. 95, 4077 (1998)
K. Muller, P. Laggner, O. Glatter, G. Kostner, Eur. J. Biochem. 82, 73 (1978)
S. Maric, T.K. Lind, J. Lyngso, M. Cardenas, J.S. Pedersen, ACS Nano 11, 1080 (2017)
E.V. Orlova, M.B. Sherman, W. Chiu, H. Mowri, L.C. Smith, A.M. Gotto, Proc. Natl. Acad. Sci. U.S.A. 96, 8420 (1999)
Y.H. Liu, D. Atkinson, J. Lipid Res. 52, 1111 (2011)
V.e.a. Kumar, PLoS One 6, e18841 (2011)
M. Golub, B. Lehofer, N. Martinez, J. Ollivier, J. Kohlbrecher, R. Prassl, J. Peters, Sci. Rep. 7, 46034 (2017)
R.J. Deckelbaum, G.G. Shipley, D.M. Small, R.S. Lees, P.K. George, Science 190, 392 (1975)
M. Pregetter, R. Prassl, B. Schuster, M. Kriechbaum, F. Nigon, J. Chapman, P. Laggner, J. Biol. Chem. 274, 1334 (1999)
W. Doster, S. Cusack, W. Petry, Nature 337, 754 (1989)
C. Mikl, J. Peters, M. Trapp, K. Kornmueller, W.J. Schneider, R. Prassl, J. Am. Chem. Soc. 133, 13213 (2011)
W. Kauzmann, Nature 325, 763 (1987)
H.L. Le Chatelier, C.R. Acad. Sci. (Paris) 99, 786 (1884)
R. Prassl, M. Pregetter, H. Amenitsch, M. Kriechbaum, R. Schwarzenbacher, J.M. Chapman, P. Laggner, PLoS ONE 3, e4079 (2008)
B. Schuster, R. Prassl, F. Nigon, M.J. Chapman, P. Laggner, Proc. Natl. Acad. Sci. U.S.A. 92, 2509 (1995)
A. Krezel, W. Bal, J. Inorg. Biochem. 98, 161 (2004)
R. Prassl, B. Schuster, P. Laggner, C. Flamant, F. Nigon, M.J. Chapman, Biochemistry 37, 938 (1998)
F. Natali, J. Peters, D. Russo, S. Barbieri, C. Chiapponi, A. Cupane, A. Deriu, M.T. Di Bari, E. Farhi, Y. Gerelli, P. Mariani, A. Paciaroni, C. Rivasseau, G. Schirò, F. Sonvico, Neutron News 19, 14 (2008)
A. Rahman, K.S. Singwi, A. Sjolander, Phys. Rev. 126, 986 (1962)
V. Réat, G. Zaccai, C. Ferrand, C. Pfister, in Biological Macromolecular Dynamics, Proceedings of a Workshop on Inelastic and Quasielastic Neutron Scattering in Biology, Institut Laue-Langevin, Grenoble, France, 1996 (Book News, Inc., Portland, OR, 1999)
V.F. Sears, Neutron News 3, 26 (1992)
J. Peters, M. Trapp, D. Hughes, S. Rowe, B. Demé, J.-L. Laborier, C. Payre, J.-P. Gonzales, S. Baudoin, N. Belkhier, E. Lelievre-Berna, High Press. Res. 32, 97 (2011)
H.H. Paalman, C.J. Pings, J. Appl. Phys. 33, 2635 (1962)
D. Richard, M. Ferrand, G.J. Kearley, J. Neutron Res. 4, 33 (1996)
J. Peters, N. Martinez, M. Trovaslet, K. Scannapieco, M.M. Koza, P. Masson, F. Nachon, Phys. Chem. Chem. Phys. 18, 12992 (2016)
J. Peters, M.T. Giudici-Orticoni, G. Zaccai, M. Guiral, Eur. Phys. J. E 36, 78 (2013)
M. Trapp, F. Juranyi, M. Tehei, L. van Eijck, B. Deme, T. Gutberlet, J. Peters, Spectroscopy 24, 461 (2010)
G. Zaccai, M. Tehei, I. Scherbakova, I. Serdyuk, C. Gerez, C. Pfister, J. Phys. IV 10, Pr7 (2000)
M. Le Bellac, Des phénomènes critiques aux champs de jauge (InterEditions/Editions du CNRS, 1988).
A. Krisko, G. Stjepanovic, G. Pifat, J.M. Ruysschaert, E. Goormaghtigh, Biochim. Biophys. Acta 1768, 2923 (2007)
H. Esterbauer, J. Gebicki, H. Puhl, G. Jurgens, Free Radic. Biol. Med. 13, 341 (1992)
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Peters, J., Martinez, N., Lehofer, B. et al. Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering. Eur. Phys. J. E 40, 68 (2017). https://doi.org/10.1140/epje/i2017-11558-8
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DOI: https://doi.org/10.1140/epje/i2017-11558-8