Figure 4
From: Cognate peptide-receptor ligand mapping by directed phage display
Panning of the HA fragment phage library with mAb 12CA5. An aliquots of eluted phage after each round of panning were analyzed by filter lifts for binding to mAb 12CA5. The filters were blocked with BSA, incubated with mAb 12CA5 and subsequently detected with goat anti-mouse kappa Ab coupled to alkaline phosphatase.