Figure 5

The effect of pre-incubating the GRP antibody with different concentrations of substance P. a, Immunostaining with the GRP antibody in lamina I-IIo from a transverse section. b-d, Equivalent fields from sections incubated in GRP antibody that had been preincubated overnight with different concentrations of substance P. Note that staining is diminished by preincubation with 10−7 M substance P, and largely absent at 10−6 and 10−5 M. Scale bar = 20 μm.