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Table 1 Percentage of misclassified reads per workflow and the number of PCR cycles. Misclassified reads included all false positives, unclassified reads, and those classified higher than the genus classification

From: Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

16S Databases

Silva

RefSeq

Workflows

Kraken2

EPI2ME

BugSeq

16S

WIMP

PCR cycles

15

2.6%

2.3%

14.3%

0.8%

20

2.7%

2.2%

14.6%

1.3%

25

2.8%

2.4%

14.7%

1.5%

30

3.4%

2.7%

14.4%

3.5%

35

4.4%

3.5%

14.3%

10.8%

  1. The Epi2me and 16S workflow had a minimum identification accuracy of 80%. PCR was conducted using an annealing temperature of 48°C, primer set#1, and LongAmp Taq polymerase
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