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Table 2 Number of genera identified per workflow and number of PCR cycles. No minimal thresholds were set

From: Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

16S Databases		Silva	RefSeq
Workflows		Kraken2	EPI2ME		BugSeq
Workflows		Kraken2	16S	WIMP	BugSeq
PCR cycles	15	71	197	252	8
	20	74	206	265	8
	25	89	211	291	8
	30	76	214	286	8
	35	84	209	261	9

The Epi2me and 16S workflow had a minimum identification accuracy of 80%. PCR was conducted using an annealing temperature of 48°C, primer set#1, and LongAmp Taq polymerase

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