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Table 3 Correlation between the expected and observed proportions of relative abundance at the genus level and the number of PCR cycles. Correlations were calculated using the Pearson’s correlation coefficient

From: Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

16S Databases		Silva	RefSeq
Workflows		Kraken2	EPI2ME			BugSeq
Workflows		Kraken2	16S		WIMP	BugSeq
PCR cycles	15	0.73	0.77	0.46		0.79
	20	0.74	0.77	0.45		0.78
	25	0.74	0.78	0.45		0.79
	30	0.74	0.77	0.47		0.75
	35	0.73	0.76	0.49		0.58

The Epi2me and 16S workflow had a minimum identification accuracy of 80%. PCR was conducted using an annealing temperature of 48°C, primer set#1, and LongAmp Taq polymerase

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