Fig. 1

High expression of the miR-23a/27a/24 − 2 cluster closely correlated with poor progression and tumor immune evasion in NSCLC. (A) Kaplan-Maier analysis showing that NSCLC patients with high expression of miR-23a/27a/24 − 2 cluster had shorter overall survival and (B) shorter disease-free survival. Analysis was performed in a cohort of 82 NSCLC patients and the log-rank test used for comparisons. (C) Representative immunohistochemical images of CD8⁺ T cells and the number of CD8⁺ T cell infiltration in human NSCLC tissues (100 × 100 μm square area). p value was calculated by t test. (D) Gene Ontology (GO) analysis was performed using mRNA sequencing data that conducted with miR-23a/27a/24 − 2 cluster silenced H1299 cells and their control cells. (E) Gene Set Enrichment Analysis (GSEA) showing that miR-23a/27a/24 − 2 cluster expression level was negatively correlated with T cell medicated immunity in NSCLC cells. GSEA was performed using mRNA sequencing results of miR-23a/27a/24 − 2 cluster silenced H1299 cells and their control cells. (F) GO analysis was performed using GEO dataset GSE151103. (G) miRNA target-based Gene Ontology analysis (http://www.microrna.gr/miRPathv4). miR-cluster, miR-23a/27a/24 − 2 cluster