Fig. 3

In situ hybridization mapping of piggyBac transformation vector insertions on chromosome 5 having the D53 inversion used in the VIENNA-8 tsl genetic sexing strain. Top: a schematic of chromosome 5 showing the piggyBac vector insertion sites along with other mapped genes and the D53 inversion breakpoints. Bottom: images of the yellow fluorescent-tagged hybridization site loci (arrows) on third larval instar salivary gland polytene chromosome spreads