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Fig. 6 | Parasites & Vectors

Fig. 6

From: Haemoproteus tartakovskyi and Plasmodium relictum (Haemosporida, Apicomplexa) differentially express distinct 18S rRNA gene variants in bird hosts and dipteran vectors

Fig. 6

Chromogenic in situ hybridization assay targeting three 18S variants of Haemoproteus tartakovskyi SISKIN1 in experimentally infected Culicoides nubeculosus (A–H) and Eurasian siskins (I–L). In Cu. nubeculosus (Cu11, 3 dpi), sporozoites (arrows) in salivary glands were labeled by the ISH probes SISKIN1-Pr1 (B) and SISKIN-Pr2 (C) but not by SISKIN-Pr3 (D), whereas oocysts were labeled by all three probes (F–H). In the Eurasian siskin, parasite blood stages (white arrowheads) were visualized in blood vessels using a Haemoproteus genus-specific probe (Haemo18S) (I). Applying the 18S variant-specific probes showed labeling of gametocytes in kidney capillaries with SISKIN1-Pr1 (J) and SISKIN1-Pr2 (K) but not with SGS1-Pr3 (L). Sg: salivary gland; h: head; fm: flight muscle; mg: midgut; ep: epidermis. Scale bars: 200 µm (A, E) and 10 µm (B–D, F–L)

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