Abstract
The approaches described here provide rapid and low-cost alternatives for sample preparation, genomic DNA extraction, and mutation discovery. When evaluating the methods, it is important to remember that protocol adaptations may be necessary to compensate for sample differences (species and genotype), environmental conditions in the laboratory, and quality of the water and chemicals used. Cost savings in DNA preparation must be balanced with the shelf-life and suitability of the samples for use in downstream applications. With the appropriate validation of sample quality and longevity, the protocols described here can provide sufficient DNA for a variety of molecular applications such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
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Keywords
- Molecular Applications
- Mutual Determination
- Downstream Applications
- Protocol Adaptations
- Sample Differences
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
The approaches described here provide rapid and low-cost alternatives for sample preparation, genomic DNA extraction, and mutation discovery. When evaluating the methods, it is important to remember that protocol adaptations may be necessary to compensate for sample differences (species and genotype), environmental conditions in the laboratory, and quality of the water and chemicals used. Cost savings in DNA preparation must be balanced with the shelf-life and suitability of the samples for use in downstream applications. With the appropriate validation of sample quality and longevity, the protocols described here can provide sufficient DNA for a variety of molecular applications such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
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© 2015 International Atomic Energy Agency
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Till, B.J., Jankowicz-Cieslak, J., Huynh, O.A., Beshir, M.M., Laport, R.G., Hofinger, B.J. (2015). Conclusions. In: Low-Cost Methods for Molecular Characterization of Mutant Plants. Springer, Cham. https://doi.org/10.1007/978-3-319-16259-1_9
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DOI: https://doi.org/10.1007/978-3-319-16259-1_9
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-16258-4
Online ISBN: 978-3-319-16259-1
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