Abstract
In this work, we report the use of unmodified gold nanoparticles (AuNPs) as an optical probe for the detection of target-responsive structural variations of DNA. By employing two DNA structures, i.e., a pH-responsive i-motif oligonucleotide and a mercury-specific oligonucleotide (MSO), we demonstrated that AuNPs could selectively distinguish target-free and target-bound oligonucleotides via the characteristic surface plasmon resonance-associated red-to-blue color change. Based on these observations, we developed a convenient “mix-and-detect” approach that could selectively detect environmentally toxic mercury ions.
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Lihua Wang is an associate professor of Shanghai Institute of Applied Physics, Chinese Academy of Sciences. She got her PhD in Shanghai Institute of Applied Physics in 2003. She is working in the area of biosensors based on novel nanomaterials and conjugated polymers.
Juan Zhang graduated from East China University of Science and Technology (BS) in 2005. She is a graduate student in Shanghai Institute of Applied Physics, Chinese Academy of Sciences now. She is focused on the development of nanobiosensors.
Wang Xun is a medical doctor in the Department of Ophthalmology of Shanghai Jiaotong University Affiliated First Hospital. She got her MD in the Second Xiangya Hospital of Central South University. Her research interests include ophthalmology and the clinical applications of nanobiosensors.
Qing Huang is a professor of Shanghai Institute of Applied Physics, Chinese Academy of Sciences. He got his PhD in Sichuan University in 2004. His research interests include neuroscience and biological effects of nanomaterials. Dun Pan is a postdoc in Shanghai Institute of Applied Physics, Chinese Academy of Sciences. He got his PhD in Technical Institute of Physics and Chemistry of CAS in 2005. His research interests include biosensors and organic synthesis.
Shiping Song is an associate professor of Shanghai Institute of Applied Physics, Chinese Academy of Sciences. He completed his PhD in chemistry in Shanghai Institute of Applied Physics in 2004. His research interests include the development and application of biosensors and protein chips.
Chunhai Fan is a professor of Shanghai Institute of Applied Physics, Chinese Academy of Sciences. He got his PhD in Nanjing University in 2000. His research interests include the development and application of nanoscale biosensors and microarrays.
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Wang, L., Zhang, J., Wang, X. et al. Gold nanoparticlebased optical probes for target-responsive DNA structures. Gold Bull 41, 37–41 (2008). https://doi.org/10.1007/BF03215621
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DOI: https://doi.org/10.1007/BF03215621