Abstract
Functional changes of cells upon developmental switches and in response to environmental cues are often reflected in nuclear phenotypes, showing distinctive chromatin states corresponding to transcriptional changes. Such characteristic nuclear shapes have been microscopically monitored and can be quantified after differential staining of euchromatin and heterochromatin domains. Here, we examined several nuclear parameters (size, DNA content, DNA density, chromatin compaction, relative heterochromatin fraction (RHF), and number of chromocenters) in relation to spatial distribution of genes and transposon elements (TEs), using standard 2D fluorescence microscopy. We provide nuclear profiles for different cell types and different accessions of Arabidopsis thaliana. A variable, yet significant, fraction of TEs was found outside chromocenters in all cell types, except for guard cells. The latter cell type features nuclei with the highest level of chromatin compaction, while their chromocenters seem to contain gene-rich regions. The highest number of parameter correlations was found in the accession Cvi, whereas Ler showed only few correlations. This may point at differences in phenotype robustness between accessions. The significantly high association of NOR chromocenters in accessions Ws and Cvi corresponds to their low RHF level.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Interphase nuclei of Arabidopsis thaliana display considerable morphological variation between cell types, developmental stages and accessions, and alters in response to external factors such as temperature, light and pathogen invasion (reviewed in Fransz and de Jong 2011; Schubert and Shaw, 2011; van Zanten et al. 2012; Zhu et al. 2015; Poulet et al. 2015; Kaiserli et al. 2018; Perrella et al. 2020). This variation can be described in terms of typical dense chromatin bodies, spatial locations of repeat sequences, epigenetic marks, and distributions of chromatin-associated proteins, including histone variants and their specific modifications. The characteristic nuclear phenotypes reflect the microscopically distinguishable classes of euchromatin and heterochromatin, originally defined by Heitz (1928). Later, the concept of heterochromatin was further extended by Brown (1966), who introduced the term “constitutive” heterochromatin to describe a permanent state of compaction, and “facultative” heterochromatin, referring to a more temporary state of heterochromatin, such as the inactivated X chromosome (Barr body) in female mammals.
Nowadays, euchromatin and heterochromatin are merely holistically characterized as chromatin states with specific epigenetic modifications associated with either active genes, repressed genes, transposable elements, or other repeats. Based on multiple profiles, a classification of chromatin subtypes has been proposed for Drosophila (Filion et al. 2010), human (Ernst et al., 2011), and Arabidopsis (Roudier et al. 2011, Sequeira-Mendes et al., 2014). Although numbers of chromatin subtypes vary between these model species, they share three main groups: (1) Euchromatin, which represents gene regions with predominantly active epigenetic marks, such as H3K4me3, H3K36me3 and histone acetylation; (2) repressive chromatin (also referred to as facultative heterochromatin), which contains mainly inactive genes, generally associated with H3K27me3 and Polycomb proteins; and (3) constitutive or C-heterochromatin, which is rich in transposons and other repetitive elements, and contains high levels of methylated H3K9 and DNA methylation (plant and human). Integration of genome-wide datasets on chromatin states in Arabidopsis, rice, and maize resulted in an extended plant chromatin state database (Liu et al. 2018).
Cytogenetic analyses of Arabidopsis nuclei stained with DNA binding fluorescent dyes have revealed characteristic patterns of chromatin types at the light microscopy level. Compact heterochromatin domains, known as chromocenters or CCs (Fransz et al. 2002), are easily identified as discrete, intensely DAPI fluorescing domains at the periphery of the nucleus and are rich in H3K9me2 and methylated DNA (Soppe et al. 2002). Such typical chromocenter patterns have been observed in dicotyledon plant species with a genome size less than 1 Gb (Ceccarelli et al. 1998; Houben et al. (2003), analyzed 24 plant species with genome sizes ranging from 170 Mb (Arabidopsis) to 43 Gb (Trillium) to assess the relation between H3K9 methylation distribution and distinct heterochromatin morphology. In the species with up to around 500 Mb (all of them dicotyledon species), the methylated H3K9 was confined to microscopically detectable heterochromatin. Rice (490 Mbp) and other (mostly monocotyledon) plant species with larger genome size exhibited dispersed labelling of the H3K9me along the entire chromatin. Euchromatin shows a more uniform pattern enriched in H3K4me3 and acetylated histones throughout the nucleus, except for the chromocenters and the nucleolus (Fransz et al. 2002; Houben et al. 2003). Repressive facultative heterochromatin is visible throughout the euchromatin region as densely speckled areas enriched with H3K27me3 and LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) (Lindroth et al. 2004; Mathieu et al. 2005; Naumann et al. 2005; Libault et al. 2005).
The distinct structures of the chromocenters provide easily quantifiable parameters for swift microscopic description of nuclear and chromatin phenotypes, which can be used to assess differences between cell types within a species, between accessions or between different species (Ceccarelli et al., 1998). To facilitate quantification of nuclear and chromatin morphometry, we introduced the term relative heterochromatin fraction (RHF), which represents the portion of total fluorescence intensity of all chromocenters relative to the fluorescence intensity of the entire nucleus (Soppe et al. 2002). Later on, Tessadori et al. (2007a) used the Heterochromatin Index (HX) to express the percentage of nuclei with a “normal” chromocenter appearance as fraction of the total population of assessed nuclei (that may include nuclei exhibiting decondensed, dispersed chromocenters). The RHF and HX parameters have been used since then to measure the dynamics of heterochromatin compaction during development and in response to biotic and abiotic stress conditions (Tessadori et al. 2007a, b; 2009; van Zanten et al. 2010, 2011; Pecinka et al. 2010; Bourbousse et al. 2015). A more sophisticated method was developed using 3D image processing technology for measuring the relative heterochromatin volume (RHV) and the position and distances between chromocenters (Andrey et al. 2010; Poulet et al. 2015, 2017; Ashenafi and Baroux, 2018; Desset et al. 2018; Arpòn et al. 2018). However, this accurate computational analysis of the 3D nuclear phenotype is in some cases less applicable for morphometric analysis, due to the time-consuming acquisition and analysis of the confocal images.
Extensive nuclear profiling of 2D cell spread images has been applied for the analysis of different chromocenter morphometric parameters, such as area, perimeter, density, roundness, and heterogeneity, to establish genetic variation between the Arabidopsis thaliana Ler and Cvi accessions and a core population of 46 recombinant inbred lines (Snoek et al. 2017). The light and temperature receptor phytochrome B was subsequently confirmed after QTL analysis as a determinative factor for nuclear size and heterochromatin organization. In spite of these diverse studies on chromocenter and nuclear phenotypes, a detailed and systematic study of nuclear and chromatin organization in different cell types across different accessions was however still lacking.
In this study, we focus on parameters that are related to the distribution of genomic DNA and heterochromatic sequences and include size, DNA content, DNA density, RHF, and number of chromocenters (CCs). We performed morphometric analysis of interphase nuclei in different cell types and organs of five Arabidopsis thaliana accessions. Based on the nuclear phenotypes, we created specific nuclear profiles for the different cell types and accessions. Such profiles shed light on the nuclear distribution of genomic elements, including the compaction of gene regions in chromocenters of guard cells and the decondensation of almost all transposons in parenchyma and pavement cells of Cvi, as well as in large nuclei of Col. This study shows that with basic tools and equipment, it is possible to quantify nuclear features for a better understanding of heterochromatin-related processes.
Materials and methods
Plant material
The Arabidopsis thaliana accessions Col (N1092), Landsberg erecta (Ler) (NW20), Wassilewskija (Ws) (Ws-2), and Cvi were obtained from the Arabidopsis Biological Resource Stock Center (ABRC, Nottingham, UK). We also used a C24 accession-derived transgenic line, carrying H2B-YFP construct that was kindly provided by F. Berger. Seeds were stratified at 4 °C for 3 days before sawing and were grown in a climate chamber under white fluorescent light (180 µmol/m2/s) in long-day photoperiods of 16 h light/8 h dark, and constant temperature (23 °C/18 °C day/night) and humidity (70%). Whole plantlets were harvested at different developmental stages, starting from stage 1.04 to 9.70 according to Boyes et al. (2001) and were then fixed with ice-cold freshly prepared ethanol/acetic acid (3:1). The material was transferred to 70% ethanol after 24 h and stored at − 20 °C until further use.
Slide preparation
Cell spread preparations were made according to the protocols of Soppe et al. (2002) and Pavlova et al. (2010). Rosette leaves and other plant organs fixed in Carnoy’s solution (ethanol/acetic acid 3:1) were washed 3 × 5 min with Milli-Q water and 1 × 5 min with 10 mM Na-citrate buffer (pH7.0) and then digested in a cocktail of pectolytic enzymes (Cytohelicase, Pall Life Sciences, Cellulose RS, Yakult Honsha Co., Ltd and Pectolyase Y23, Sigma) in a final concentration of 0.1% (for each enzyme) in Na-citrate buffer for 3 h at 37 °C. The digested material was transferred to water and chopped with a needle to a fine suspension. A droplet of 5 µL suspension was mixed with 20–40 µL 45% acetic acid and spread on a slide for 15 s on a hotplate at 43 °C. Extra washes in ice-cold acetic acid–ethanol (3:1) were carried out, followed by air-drying and storing at 4 °C. The nuclei were stained by 4’,6-diamidino-2-phenylindole (DAPI, 4 μg/mL), propidium iodide (PI, 4 μg/mL), or SYTOX® Green (35 nM, Invitrogen), and slides were mounted in Vectashield (Vector Laboratories, Burlingame, CA, USA) before observation. For the PI-stained slides, we incubated in 100 mg/mL RNAse A (Roche, the Netherlands) for 1 h at 37 °C prior to the DNA staining.
FISH
Fluorescence in situ hybridization (FISH) experiments were carried out as described by Lysak et al. (2006) with slight modifications. Chromosome preparations were dried by overnight incubation at 37 °C. Slides were subsequently treated with RNAse (100 µg/mL in 2 × SSC) for 1 h at 37 °C and rinsed in 2 × SSC buffer for 3 × 5 min. Next, the material was fixed in 1% (w/v) (para)formaldehyde in PBS buffer (10 mM sodium phosphate buffer, pH 7.0, 143 mM NaCl) for 10 min and rinsed in 2 × SSC for 3 × 5 min, dehydrated through an ethanol series (70%, 90%, and 100%, each 2 min), and subsequently air-dried. To each slide 20 µL of hybridization mix, containing 100 ng probe in 50% formamide, 2 × SSC, 50 mM sodium phosphate (pH 7.0), and 10% sodium dextran sulfate, was added. The slide was subsequently denatured on a hot block set at 80 °C, for 2.5 min. The slides were incubated in a moist chamber at 37 °C for 18 h. Post-hybridization washes were performed in 50% formamide, 2 × SSC (pH 7.0) for 3 × 5 min at 42 °C, followed by 2 × SSC at room temperature for 3 × 5 min, dehydration through an ethanol series and staining with 2 µg/mL DAPI on slides. 45S rDNA was used as a probe (Gerlach and Bebrook, 1979) and directly labelled with DEAC-dUTPs using a standard Nick-translation kit (La Roche).
Image acquisition and processing
Fluorescence microscopy
Slides were examined using a Zeiss Axioplan 2 Photomicroscope equipped with N.A. 1.4 Plan-Apochromatic objectives, epi-fluorescence illumination and appropriate small band filter sets for DAPI, propidium Iodide, Sytox Green, and DEAC fluorophores. We captured 12-bit raw images with a Photometrics Sensys 1305 × 1024 pixels CCD camera using the Genus Image Analysis Workstation software (Applied Imaging Corporation). Exposure of the images was set to control the full dynamic range of the image by moving the black and white point switches left and right of the image display curve for optimal contrast (black) and brightness (white). Images were saved as 8-bit RGB TIFF files for quantitative analysis with the freeware Mac OSX software Object Image (modified as https://www.quantitative-plant.org/software/objectj, the ImageJ plugin CHIAS (http://www2.kobe-u.ac.jp/~ohmido/index03.htm) and Image Pro Plus v.5 (http://www.mediacy.com/). FISH images were stored for each fluorescence signal separately and merged in Adobe Photoshop multilayer images using different blend modes (Kantama et al. 2017).
Confocal laser scanning microscopy
Twelve-bit images were recorded using an LSM 510 confocal laser scanning microscope (Carl Zeiss, Göttingen, Germany) equipped with a 63 × /1.4 NA Plan Apochromatic objective. We used an Argon ion laser at 364 and 488 nm and a He/Ne laser at 543 nm for detecting DAPI (385–470 nm band pass filter) and FITC (505–530 nm band pass filter), respectively. Images were scanned as 512 by 512 × 9 voxel images with a sampling rate of 140 × 140 × 700 nm (x, y, z).
Image processing and analysis
In order to semi-automatically analyze high numbers of raw (unprocessed) images, we applied our in-house developed macro in ImagePro-Plus (Media Cybernetics, Silver Spring, MD, USA) for the morphometric analysis to segment the nuclei and measure size (number of pixels) and fluorescence intensity of the interphase nucleus and individual chromocenters by a threshold algorithm (Pavlova et al. 2010). We monitored the biological parameters size, DNA density, DNA content, variation in DNA density, relative heterochromatin fraction (RHF), and number of chromocenters (see Table 1 for detailed description). Thirty to one hundred nuclei per sample were examined in every experiment. Data were exported to a Microsoft Excel spreadsheet and further analyzed using the open-source statistical program JASP (JASP Team, 2020, Version 0.13.01, Computer software, https://jasp-stats.org). Comparisons were evaluated by a one-way ANOVA test or a t-test.
For the calculation of the fraction of transposon elements (TEs) in chromocenters or euchromatin, we reasoned as follows. The total amount of TEs was estimated at 10% (Arabidopsis Genome Initiative 2000). The total amount of the centromeric 180 bp arrays was estimated at 8.76 Mb, based on pachytene FISH analysis (Haupt et al. 2001). For the total size of NOR domains, we used the value of 7.5 Mb based on CHEF gel analysis of the 45S ribosomal gene arrays (Copenhaver and Pikaard 1996). Although previous studies have shown significant copy number variation for the 45S ribosomal gene arrays, in particular from Swedish accessions (Long et al., 2013), the 45S rDNA copy number variation in the accessions used in our study corresponds to less than 1% of the total genome size (Woo and Richards, 2008). We did not include the 5S ribosomal repeats, spanning in total about 0.5 Mb (Campell et al., 1992), since this array is often found outside chromocenters (Mathieu et al., 2003; Tessadori et al., 2007a). The fraction of TEs in CCs is calculated as follows:
The total amount of 45S and 180 bp DNA represents the amount of major tandem repeats (19.3 Mb). The product of RHF and genome size equals the amount of DNA in the CC. The total amount of TE is 10% of the genome size. The calculation also involves the genome size of Arabidopsis, which differs between several studies. Therefore, we used in this study four estimates for the genome size: 125 Mb (Arabidopsis Genome Initiative, 2000), 157 Mb (Bennet et al., 2003), 191 Mb (Doležel et al., 1998), and 211 Mb (Schmuths et al., 2004). The fraction of TEs in euchromatin is determined by subtracting the fraction TEs in CC from 1.
Results and discussion
Comparison of software tools and DNA dyes for morphometric analysis
Accurate analyses of nuclear morphometric parameters rely on the usage of appropriate techniques and choices made, such as the preferred DNA stain, the image acquisition platform, and the computational image tools. In addition, quantification of heterochromatin requires proper segmentation of condensed, stained areas in the nucleus, whereby thresholding (and thus resolution and resolving power) is critical.
We assessed three software packages; Object Image (Vischer et al. 1999), CHIAS (Fukui, 1986, 2005), and our in-house developed Image Pro + macro (Pavlova et al., 2010), for simple and rapid quantification of the relative heterochromatin fraction (RHF) of 2D images of interphase nuclei, to estimate their accuracy in data measurements. The RHF values of three independent sets of nuclei (~ 30 nuclei/set) with different heterochromatin compactions were determined with each software package (Fig. 1A). The values of the three sets displayed a similar pattern when analyzed by the three programs: set 2 was significantly lower than set 3 and that was significantly lower than set 1. Although the values for the sets 1, 2, and 3 obtained with Object Image and Image Pro + were comparable (p = 0.417), those with CHIAS were about four times lower (p < 0.01). These differences in RHF values can be explained by the way thresholding of the “objects” is determined. But overall, it is clear that the three image analysis programs produce comparable accurate measurements of relative differences in heterochromatin content between different datasets, if analyzed with the same software. Care should however be taken when comparing datasets obtained with different analysis (software) methods. In our experiments, we preferred to use the Image Pro + macro tool as it enables us to analyze large data sets with high accuracy and with a wide selection of quantification parameters.
Nuclear morphometric analyses of 2D nuclei are based on pixel intensity measurements of stained (fluorescent) DNA. Diverse processes may affect the interaction between the dye and the DNA structure (e.g., minor or major groove) or the affinity of the dye for certain DNA (RNA) sequences (e.g., sequences enriched AT content) (Schweizer 1976). We therefore compared the RHF values obtained from nuclei stained with different DNA-specific fluorophores dyes: DAPI (emission: 461 nm, spatial resolution 0.24 µm), SYTOX®-Green (533 nm, 0.25 µm), and propidium iodide (636 nm, 0.33 µm), as these fluorophores are commonly used in DNA visualization, chromosome staining or flow cytometry (Williamson and Fennell, 1979; Russell et al. 1975; Fried et al. 1976; Hulett et al. 1969). Since PI has affinity to both DNA and RNA, we treated the slides with RNase prior to staining. Images of parenchyma and pavement nuclei were captured by fluorescent microscopy and the RHF was calculated with Image Pro + . We observed that mean values for RHF of Sytox green are 8% higher than that of DAPI, PI 14% higher than that of DAPI, and PI 5% higher than that of Sytox green (Fig. 1B). The differences were however not significant (ANOVA, p = 0.1). These slight differences might be explained by a combination of DNA/RNA, and AT/GC specificity, binding to both DNA and core histones (Banerjee et al. 2014) and wavelength dependent optical resolution of the objective. We decided to work with DAPI in the remainder of our experiments, because of slightly higher optical resolution, double-strand DNA specificity (no RNase treatment needed), relatively low fluorescence fading, and the limited cross-talk with other narrow-band fluorescence filter sets.
Cell type–specific profiling of nuclear morphology
Plant leaves change in composition and function during development. Since nuclei in differentiated, old leaves contain a high heterochromatin fraction (Tessadori et al. 2004) and exhibit increased endopolyploid levels (Del Prete et al. 2019), we decided to focus on young, ~ 5-mm-long rosette leaves of stage 1.05 (Boyes et al. 2001). Confocal microscopy images of transgenic plants expressing H2B-YFP protein (in the C24 genetic background) revealed clear differences in size and shape of nuclei between distinct cell types derived from different leaf tissues (Fig. 2). Nuclear isolation followed by spread preparation and DAPI staining yielded a mixed population of distinctive leaf nuclei. We sorted the nuclei based on nuclear size and nuclear shape and grouped them using microscopic observations as follows: (1) small and spherical, (2) medium-sized and elliptic/irregular shape, (3) elongated, (4) large. We assigned those as nuclei of (1) guard cell (GC), (2) parenchyma/pavement cell (PC), (3) vascular cell (VC), and (4) endopolyploid cell (EC). We based this classification on 3D data (Fig. 2) and information from the literature (Traas et al., 1998, Schubert et al., 2012, Del Prete et al., 2014, Poulet et al., 2017). However, based on this qualitative data alone, we cannot fully exclude that some parenchyma cells for instance have small, round nuclei. Of note, EC have replicated their DNA at least once, without undergoing cell division (Leitch and Dodsworth, 2017), although a diploid nucleus in G2-phase is difficult to distinguish from a tetraploid nucleus in G1.
Quantification of morphometric parameters (Table 1) revealed clear differences in nuclear features between the cell types (Fig. 3A). GC nuclei are the most remarkable with a round nuclear phenotype with a diameter of 4.84 µm. Their volume is estimated at 18 µm3, which matches the volume of the GC nucleus in 3D microscopic images (Poulet et al. 2017). Also, the size ratio between GC nuclei and PC nuclei is in agreement with other studies (Kato and Lam 2003; Poulet et al. 2017). Compared to the other cell types, GC nuclei contain about two times less DNA content and are 2–3 times smaller. They display the highest DNA density in euchromatin and in heterochromatin, which corresponds to a higher concentration of DNA. Guard cell nuclei also show the highest RHF (= 0.19). In comparison, the other cell types have RHF values varying from 0.095 to 0.13. The large CC/nucleus size ratio of GC cells further contributes to the typical morphological appearance of condensed chromatin. In addition, the high heterogeneity points at large variation in DNA compaction and endorses the sharp boundaries between the heterochromatin chromocenters and the surrounding euchromatin in the guard cell nuclei. In contrast, the PC, EC, and VC nuclei show less variation in heterogeneity, suggesting a more uniform distribution of DNA inside the nucleus. Additionally, the relatively low number of distinguishable chromocenters (5.5 per nucleus) implies that chromocenters are frequently associated in guard cell nuclei. The high DNA density in GC nuclei approximates the DNA density in chromocenters of PC nuclei and points to high chromatin compaction in the GC nucleus. This may have consequences for gene activity, since compaction leads to reduced mobility and hence to less interaction between distal chromosomal regions. Indeed, Kato and Lam (2003) detected with LacI-GFP that the confinement areas of the tagged loci with repetitive LacO arrays are six times smaller in GC nuclei compared to PC nuclei. Hence, the chromatin movement area in GC nuclei is apparently restricted, which is in accordance with the high chromatin compaction in the GC nucleus in our study.
The product of DNA density and area represents an estimate of DNA content from which the approximate C-value of the nuclei can be calculated. We worked from the assumption that guard cell nuclei have a DNA level of 2C (Melaragno et al. 1993) and estimated that pavement and parenchyma cells have on average 4C, vascular cells 5C, and endopolyploid cells 6.7C. Consequently, the majority of the diploid GCs are in the G0,1 phase of the cell cycle and are not endoreduplicated, while PC nuclei are predominantly in G2, although endoreduplication cannot be excluded. The polyploid DNA levels in PC, VC, and EC and their lower DNA density (compared to GC) suggest more chromatin accessibility in cells with higher ploidy levels. A similar correlation between chromatin conformation and ploidy level was reported in other studies (Kato and Lam, 2003; Schubert et al., 2012). The higher accessibility of chromatin in polyploid nuclei suggests that more gene copies in endoreduplicated cells facilitate more transcripts in specialized cells.
We subsequently examined correlations between the morphometric parameters in a pairwise manner and generated correlation heatmaps (Fig. 4A) and matrices (Fig. 4B) for each cell type. Since euchromatin is the prevailing chromatin fraction in the Arabidopsis nucleus (> 80%), we did not include euchromatin parameters and focused on the correlation between nuclear (nu) and chromocenter (CC) parameters. The amount of DNA in the nucleus is significantly (p < 0.001) related to the DNA content in CCs, which suggests that the quantitative partition of DNA into euchromatin and chromocenters is maintained at higher ploidy levels. The pairwise correlation shows that nuclei of guard cells are different compared to nuclei from the other cell types in having weaker correlations between the morphometric parameters. In some cases, GC nuclei show greater similarity to EC. For example, heterogeneity is significantly related to DNA density in chromocenters of PC and VC (coeff. 0.8–0.9, p < 0.001), but less, yet still significantly, in GC and EC (coeff. 0.5–0.6, p < 0.01). Heterogeneity is a measure for the variation in DNA density in euchromatin and heterochromatin and, hence, is more dependent on the level of DNA condensation in both chromatin domains. For DNA density in the nucleus and DNA density in chromocenters, we observed the expected similar high levels of correlation in PC and VC vs less in GC and EC. This may be explained by the fact that PC and VC show similar values for most of the nuclear parameters, whereas GC and EC are often significantly different (Fig. 3A). A principal component analysis of the four groups of nuclei revealed four different clusters in which the GC cluster is more separated from the other three clusters and more opposite to the EC cluster (Fig. 2B).
In order to assess intraspecific morphometric and genetic variation in nuclear and chromatin phenotypes, we quantified morphometric parameters of nuclei isolated from different organs, i.e., anthers, cauline leaf, ovarium, petals, root, rosette leaf, sepals, and stem. As expected, many parameters varied between the different organs, such as size, DNA density, DNA content, and heterochromatin content (RHF) of the nucleus and DNA density of the CC (Fig. 3B). Nuclei of anthers, petals, and roots have the smallest nuclei, the highest RHF, and the lowest DNA content, with almost similar values as guard cells, although these tissues lack guard cells (Pillitteri et al. 2008). The nuclear phenotypes of the organs reflect the presence of mixed tissues with different cell types. In conclusion, our morphometric analysis shows that measuring area and pixel intensities of flattened 2D nuclei preparations and components thereof (i.e., chromocenters) allows profiling of different types of nuclei and enables detailed analysis of dynamic interrelated nuclear features.
Arabidopsis accessions differ in nuclear morphometry
Arabidopsis thaliana is native to diverse climates throughout the Northern hemisphere and accessions have adapted to their local environment (Hancock et al., 2011). We previously demonstrated that chromatin compaction values correlate with geographical latitude of origin within a diverse panel of natural accessions (Tessadori et al. 2009). Subsequent genetic analyses indicated that light intensity is a major determinant of chromatin (de)condensation (Tessadori et al. 2009; van Zanten et. al. 2010; Snoek et al. 2017). Here we extended on this analysis, by considering nuclear morphometry using nuclei from parenchyma and pavement cells (PC) of the accessions C24, Col, Cvi, Ler, and Ws-2. A significant difference was found in the RHF (p < 0.01) between the accessions, with Col and Ler having the highest RHF (0.13) while the lowest RHF (0.08) is found for Cvi (Fig. 5A). These observed RHF values match well with the results from our previous studies on RHF (Soppe et al. 2002; Tessadori et al. 2007a, b; Snoek et al. 2017) and HX (Tessadori et al., 2009).
Overall, the accessions C24 and Ws show comparable morphometric features, including nuclear size, nuclear DNA content, DNA content in CCs, and heterogeneity, in which they differ from the other accessions. The two accessions differ however from each other in DNA density in CCs and for the compound trait RHF, which is significantly lower in Ws-2 than in C24 (Fig. 5A). This indicates that small differences in individual parameters can have a profound effect on overall nuclear appearance, when combined. The relatively high DNA content suggests that C24 and Ws have more PC nuclei in the G2 or early endoreduplicated phase of the cell cycle (= 4C) than the Col, Cvi, and Ler accessions under the tested conditions and developmental state. The ~ 1.5 times larger nuclear volume of C24 and Ws supports this conclusion. A principal component analysis of the five accessions assigned C24 and Ws to a cluster and Col, Cvi, and Ler to another cluster, further supporting the similarity between C24 and Ws based on the morphometric features (Fig. 5B).
Subsequently, we generated correlation heatmaps and matrices (Fig. 6) to visualize correlations between morphometric parameters for each accession. The number of chromocenters, which is similar (7–8) for all accessions, is not or only weakly correlated to the other parameters (Suppl. Figure S2). Apparently, the process of chromocenter association in parenchyma/pavement cells occurs independently of DNA content or size of the nucleus. Overall, the correlations between nuclear size and DNA content are strong in Cvi, moderate in Ws, Col and Ler, and weak in C24. Since the feature DNA density, which is equivalent to DNA concentration, can be defined as DNA amount per volume, the correlation between nuclear size and DNA content can be reflected in the DNA density parameter. An increase in DNA content, for example, by DNA replication, enlarges the nuclear size, but not the DNA density in Cvi, Ws, Col, and Ler. In contrast, in C24, an increase in DNA content is accompanied with a moderate increase of the DNA density and a weak increase of the nuclear size. The C24 accession also differs from the other accessions in having a sharp boundary of the chromocenters. In comparison, Ws-2 and Cvi exhibit more diffuse CCs showing a more gradual transition between heterochromatin and euchromatin.
All accessions show strong correlations (coeff. > 0.84) between CC size and DNA content of CC, which suggests that an increase in heterochromatin does not primarily results in more compaction of CCs, but rather in enlargement of the CC domains. Hence, the observed values for the DNA density in CC is possibly the upper limit in PC cells of these accessions. DNA density in CCs was strongly correlated (coeff. = 0.80) only with nuclear DNA density in Cvi. This accession has the lowest DNA density in CC (Fig. 5A) and also displays the lowest RHF value, probably due to “low light stress” that this sub-tropical accession (latitude 15.1 oN) experiences under our greenhouse conditions of the relatively northern city of Amsterdam (latitude 52.4 oN) (Tessadori et al. 2009). Interestingly, Cvi is the accession with overall the strongest correlations, showing significantly high levels of pairwise correlations between many nuclear parameters (Fig. 6A). In contrast, relatively few parameter correlations are found in Ler. This may indicate that the nuclear phenotype of Ler is more robust: a change in a certain parameter has limited effect on another parameter. The high correlation values in Cvi indicate that changes in several parameters may influence each other and suggest that nuclear organization in Cvi is more plastic in the tested conditions.
Chromocenters CC2 and CC4 accommodate nuclear organizing regions (NORs) containing ribosomal gene arrays and associate most frequently with each other or with other CCs (Fransz et al. 2002; Pecinka et al. 2004). To elaborate on this result and the observed striking variation in CC condensation between the accessions, we tested for a correlation between nuclear morphology and the association of CC2 and CC4. We applied FISH with a 45S rDNA probe and counted the number of fluorescent signals per nucleus (Fig. 7). The results indicate that accessions have different preferences for association of the NOR CCs. Ws and Cvi displayed significantly less 45S rDNA signals than the other tested accessions. These two accessions also exhibit lower RHF values compared to Col, Ler, and C24. Considering a similar average number (7–8) of chromocenters in all accessions, it is therefore likely that in Ws-2 and Cvi, the NOR chromocenters are preferentially associated. Interestingly, these two accessions have a much lower level of rDNA methylation compared to Col, Ler, and C24 (Woo and Richards, 2008), which is in line with their low RHF. These data suggest a mechanistic link between DNA methylation level and the association of heterochromatic NOR domains. We speculate that less DNA methylation of tandem repeats correlates with more association of the corresponding heterochromatin domains.
Fraction of TEs in CC and euchromatin
The RHF, which represents the fraction of genomic DNA in CCs, allows to estimate the fraction of transposable elements (TEs) in chromocenters. From previous studies, it is known that long tandem repeat arrays, such as ribosomal gene regions and the centromeric 180 bp repeat regions, are stable components of CCs, while transposon-rich regions and 5S rDNA repeats can easily disperse from the CC territories (Mathieu et al., 2003; Tessadori et al. 2007a, b). The long tandem repeats 45S rDNA and 180 bp decondense only under exceptional conditions, such as complete dedifferentiation during protoplast isolation (Tessadori et al., 2007a), or in the strong chromatin remodelling mutant ddm1-5 (Mittelsten Scheid et al. 2002). It follows that the fraction of TEs in CCs can be estimated by subtracting the DNA representing the long tandem repeats from the total DNA present in the CC (see M&M for the calculation). We calculated the fraction of TE in CC for the group of cell types and the group of accessions (Table 2). The fraction of TE in the CC is highest in GC nuclei, which is not surprising, since these cells have the highest RHF (0.19). Remarkably, in several cell types and accessions, we observe a negative value for the fraction of TE in CC, which is the case when the amount of DNA in CC (= genome size x RHF) is lower than the amount of major tandem repeats (= 16.3 Mb). A combination of a low RHF and a low genome size is responsible for the negative values of the fraction of TE in CC. This negative value implies that not only all TEs are outside the CCs, but also a fraction (several megabases) of the major tandem repeats is no longer part of heterochromatin. We have never observed decondensation of major tandem repeats in plants under normal conditions, but only under high stress, such as protoplast formation or in the mutant ddm1-5 (Tessadori et al., 2007a; Mittelsten Scheid et al., 2002). Therefore, we suggest that the genome size is too low if the fraction of TE in CC becomes less than zero in plants grown under non-stress conditions. This is the case for the genome sizes 125 Mb, 157 Mb, and perhaps 191 Mb. Only for the estimated genome size 211 Mb did we find no negative values for the fraction of TE in CC.
In case the genome size of Arabidopsis is 211 Mb, we can infer that in PC (in Col, Ler, and C24), VC, and GC a significant fraction (> 40%) of TEs is found in the CCs. The only cell type that did not show a negative value for the fraction of TE in CC for any of the genome sizes is the GC, which has the highest RHF (0.19). For the GC, we calculated a TE fraction (1.13) in CCs. This fraction is greater than 1.0, which implies a DNA amount in CCs that is higher than the total amount of genomic repeats (i.e., tandem repeats plus TEs). We conclude from this that in guard cells, not only the tandem repeats and all TEs is allocated in the CCs, but also a substantial fraction of gene-rich regions. These results match with the observation that guard nuclei have highly condensed chromatin, both in euchromatin and in CCs (see above).
The endopolyploid cells appear to have the majority (87%) of TEs in euchromatin, which supports the idea that endoreduplicated nuclei have more accessible chromatin for transcription. The PC nuclei of the accession Cvi has the highest percentage (97%) of TEs in euchromatin domains, which is in agreement with the highly decondensed appearance of this accession under our climate chamber conditions (Tessadori et al., 2009). Ws also displays a major fraction (77%) of TEs outside the chromocenters. Assuming a genome size of 211 Mb, we estimate that all repeats (tandem arrays and TEs) are in CCs when the RHF is 0.18 or higher while all TEs are in euchromatin when the RHF is 0.07 or lower (Suppl Table S1).
Conclusions
Our microscopic study demonstrates that 2D image analysis based on pixel number and DAPI fluorescence intensity enables detailed assessment of nuclear morphometric profiles of different cell types and accessions. These profiles shed light on genomic elements in the nuclear context, elucidating the obvious compaction of gene regions into chromocenters of guard cell nuclei and the decondensation of a greater part of the transposons in endopolyploid cells of Col and in parenchyma and pavement cells of Cvi. Differences between cell types and accessions may be related to genomic and epigenetic variation in the rDNA loci between the accessions. The type of morphometric analyses and correlation profiling as presented in this study constitute an important step towards further local and global analysis on gene activity, DNA methylation in ribosomal genes, and assessment of robustness of biotic and abiotic stress tolerance in genomes. Therefore, we propose morphometric analysis as a swift and easy tool to estimate genome integrity of plant subjected to environmental stress.
Data availability
Not applicable.
Code availability
Not applicable.
Change history
28 December 2021
A Correction to this paper has been published: https://doi.org/10.1007/s10577-021-09677-y
Abbreviations
- CC:
-
Chromocenter
- DAPI:
-
4’,6-Diamidino-2-phenylindole
- EC:
-
Endopolyploid cell
- Euchr:
-
Euchromatin
- GC:
-
Guard cell
- HX:
-
Heterochromatin index
- NOR:
-
Nucleolar organizer region
- PC:
-
Pavement/parenchyma cell
- PI:
-
Propidium iodide
- RHF :
-
Relative heterochromatin fraction
- TE:
-
Transposable element
- VC:
-
Vascular cell
References
Andrey P, Kiêu K, Kress C, Lehmann G, Tirichine L, Liu Z, Biot E et al (2010) Statistical analysis of 3D images detects regular spatial distributions of centromeres and chromocenters in animal and plant nuclei. PLoS Comput Biol 6(7):e1000853. https://doi.org/10.1371/journal.pcbi.1000853.t002
Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815
Arpòn A, Gaudin V, Andrey P (2018) A method for testing random spatial models on nuclear object distributions. In: Methods Mol Biol 2018;1675:493–507. doi: https://doi.org/10.1007/978-1-4939-7318-7_29
Ashenafi MS, Baroux C (2018) Automated 3D gene position analysis using a customized Imaris Plugin: XTFISHInsideNucleus. Methods Mol Biol 1675:591–614. https://doi.org/10.1007/978-1-4939-7318-7_32
Banerjee A, Majumder P, Sanyal S, Singh J, Jana K, Das C, Dasgupta D (2014) The DNA intercalators ethidium bromide and propidium iodide also bind to core histones. FEBS Open Bio 4 (C). Federation of European Biochemical Societies: 251–59https://doi.org/10.1016/j.fob.2014.02.006
Bennett MD, Leitch IJ, Price HJ, Johnston JS (2003) Comparisons with Caenorhabditis (∼100 Mb) and Drosophila (∼175 Mb) using flow cytometry show genome size in Arabidopsis to be ∼157 Mb and thus ∼25 % larger than the Arabidopsis genome initiative estimate of ∼125 Mb. Ann Bot 91(5):547–557. https://doi.org/10.1093/aob/mcg057
Boyes D, Zayed A, Ascenzi R, McCaskill A, Hoffman N, Davis K, Gerlach J (2001) Growth stage–based phenotypic analysis of Arabidopsis: a model for high throughput functional genomics in plants. Plant Cell 13:1499–1510
Bourbousse C, Mestiri I, Zabulon G, Bourge M, Formiggini F, Koini MA et al (2015) Light signaling controls nuclear architecture reorganization during seedling establishment. Proc Natl Acad Sci USA 112(21):E2836–E2844. https://doi.org/10.1073/pnas.1503512112
Brown S (1966) Heterochromatin provides a visible guide to suppression of gene action during development and evolution. Science 151:417–425
Campell BR, Song Y, Posch TE, Cullis CA, Town CD (1992) Sequence and organization of 5S ribosomal RNA-encoding genes of Arabidopsis thaliana. Gene 112:225–228
Ceccarelli M, Morosi L, Gionini PG (1998) Chromocenter association in plant cell nuclei: determinants, functional significance, and evolutionary implications. Genome 41:96–103
Copenhaver GP, Pikaard CS (1996) RFLP and physical mapping with an rDNA-specific endonuclease reveals that nucleolus organizer regions of Arabidopsis thaliana adjoin the telomeres on chromosomes 2 and 4. Plant J 9(2):259–272
Del Prete S, Arpón J, Sakai K, Andrey P, Gaudin V (2014) Nuclear architecture and chromatin dynamics in interphase nuclei of Arabidopsis thaliana. Cytogenet Genome Res 143:28–50. https://doi.org/10.1159/000363724
Del Prete S, Molitor A, Charif D, Bessoltane N, Soubigou-Taconnat L, Guichard C, Brunaud V, Granier F, Fransz P, Gaudin V (2019) Extensive nuclear reprogramming and endoreduplication in mature leaf during floral induction, BMC Plant Biology: 1–19https://doi.org/10.1186/s12870-019-1738-6
Desset S, Poulet A, Tatout C (2018) Quantitative 3D analysis of nuclear morphology and heterochromatin organization from whole-mount plant tissue using nucleus. J Methods Mol Biol 1675:615–632. https://doi.org/10.1007/978-1-4939-7318-7_33
Doležel J, Greilhuber J, Lucretti S, Meister A, Lysak MA, Nardi L, Obermayer R (1998) Plant genome size estimation by flow cytometry: inter-laboratory comparison. Annals of Botany 82(Suppl A):17–26
Ernst, Jason, Pouya Kheradpour, Tarjei S Mikkelsen, Noam Shoresh, Lucas D Ward, Charles B Epstein, Xiaolan Zhang, et al. 2011. Mapping and analysis of chromatin state dynamics in nine human cell types. Nature 473 (7345). Nature Publishing Group: 43–49. doi:https://doi.org/10.1038/nature09906
Filion GJ, van Bemmel JG, Braunschweig U, Talhout W, Kind J, Ward LD, Brugman W et al (2010) Systematic protein location mapping reveals five principal chromatin types in Drosophila cells. Cell 143(2):212–224. https://doi.org/10.1016/j.cell.2010.09.009
Fransz P, de Jong H, Lysak M, Castiglione MR, Schubert I (2002) Interphase chromosomes in Arabidopsis are organized as well defined chromocenters from which euchromatin loops emanate. PNAS 99:14584–14589
Fransz P, Soppe W, Schubert I (2003) Heterochromatin in interphase nuclei of Arabidopsis thaliana. Chromosome Res 11:227–240
Fransz P, de Jong H (2011) From nucleosome to chromosome: a dynamic organization of genetic information. Plant J 66(1):4–17. https://doi.org/10.1111/j.1365-313X.2011.04526.x
Fried J, Perez AG, Clarkson BD (1976) Flow cytometric analysis of cell cycle distributions using propidium iodide. Properties of the method and mathematical analysis of the data. J Cell Biol 71:171–181
Fukui K (1986) Standardization of karyotyping plant chromosomes by a newly developed chromosome image analyzing system (CHIAS). Theor Appl Genet 72:27–32
Fukui K (2005) Recent development of image analysis methods in plant chromosome research. Cytogenet Genome Res 109:83–89
Gerlach W, Bebrook J (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7(7):1869–1885
Hancock AM, Brachi B, Faure N, Horton MW, Jarymowycz LB, Sperone FG, Toomajian C, Roux F, Bergelson J (2011) adaptation to climate across the Arabidopsis thaliana genome. Science 334(6052):83–86. https://doi.org/10.1126/science.1209244
Haupt W, Fischer TC, Winderl S, Fransz P (2001). The centromere1 (CEN1) region of Arabidopsis thaliana: architecture and functional impact of chromatin. The Plant Journal (2001) 27(4): 285–296
Heitz E (1928) Das Heterochromatin der Moose. I Jahrb Wiss Botanik 69:762–818
Houben A, Demidov D, Gernand D, Meister A, Leach CR, Schubert I (2003) Methylation of histone H3 in euchromatin of plant chromosomes depends on basic nuclear DNA content. Plant J 33:967–973. https://doi.org/10.1046/j.1365-313X.2003.01681.x
Hulett HR, Bonner WA, Barrett J, Herzennberg LA (1969) Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence. Science 166:747–749
Kaiserli E, Perrella G, Davidson MLH (2018) Light and temperature shape nuclear architecture and gene expression. Current Opinion in Plant Biology 45 (Part A): 103–11 https://doi.org/10.1016/j.pbi.2018.05.018
Kantama L, Wijnker E, de Jong H (2017) Optimization of cell spreading and image quality for the study of chromosomes in plant tissues. In: Anja Schmidt (ed.), Plant germline development: methods and protocols, Methods in Molecular Biology 1669: 141–158. DOI https://doi.org/10.1007/978-1-4939-7286-9_12
Kato N, Lam E (2003) Chromatin of endoreduplicated pavement cells has greater range of movement than that of diploid guard cells in Arabidopsis thaliana. J Cell Sci 116:2195–2201
Leitch IJ, Dodsworth S (2017). Endopolyploidy in plants. In: eLS. John Wiley & Sons, Ltd: Chichester. https://doi.org/10.1002/9780470015902.a0020097.pub2
Libault M, Tessadori F, Germann S, Snijder B, Fransz P, Gaudin V (2005) The Arabidopsis LHP1 protein is a component of euchromatin. Planta 222:910–925
Lindroth AM, Shultis D, Jasencakova Z, Fuchs J, Johnson L, Schubert D, Patnaik D, Pradhan S, Goodrich J, Schubert I et al (2004) Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3. Embo J 23:4286–4296
Liu Y, Tian T, Zhang K, You Q, Yan H, Zhao N, Yi X, Xu W, Su Z (2018) PCSD: a plant chromatin state database. Nucleic Acids Res 46:D1157–D1167. https://doi.org/10.1093/nar/gkx919
Long Q, Rabanal FA, Meng D, Huber CD, Farlow A, Platzer A, Zhang Q, Vilhjálmsson BJ, Korte A, Nizhynska V, Voronin V, Korte P, Sedman L, Mandáková T, Lysak MA, Seren Ü, Hellmann I, Nordborg M (2013) Massive genomic variation and strong selection in Arabidopsis thaliana lines from Sweden. Nat Genet 45:884–890
Lysak M, Fransz P, Schubert I (2006) Cytogenetic analyses of Arabidopsis. Methods Mol Biol 323:173–186
Mathieu O, Vaillant I, Gendrel A-V, Colot V, Schubert I, Tourmente S (2003) Changes in 5S rDNA chromatin organization and transcription during heterochromatin establishment in Arabidopsis. Plant Cell 15:2929–2939
Mathieu O, Probst AV, Paszkowski J (2005) Distinct regulation of histone H3 methylation at lysines 27 and 9 by CpG methylation in Arabidopsis. The EMBO Journal 3;24(15):2783–2791. https://doi.org/10.1038/sj.emboj.7600743.
Melaragno JE, Mehrotra B, Coleman AW (1993) Relationship between endopolyploidy and cell size in epidermal tissue of Arabidopsis. Plant Cell 5(11):1661–1668
Mittelsten Scheid O, Probst AV, Afsar K, Paszkowski J (2002) Two regulatory levels of transcriptional gene silencing in Arabidopsis. PNAS 99 (21) 13659–13662 https://doi.org/10.1073/pnas.202380499
Naumann K, Fischer A, Hofmann I, Krauss V, Phalke S, Irmler K, Hause G, Aurich AC, Dorn R, Jenuwein T, Reuter G (2005) Pivotal role of AtSUVH2 in heterochromatic histone methylation and gene silencing in Arabidopsis. EMBO J 24:1418–1429
Pavlova P, Tessadori F, de Jong H, Fransz P (2010) Immunocytological analysis of chromatin in isolated nuclei. Plant developmental biology, Methods in Molecular Biology 655, L. Hennig, C. Köhler (eds.), DOI https://doi.org/10.1007/978-1-60761-765-5_28
Pecinka A, Schubert V, Meister A, Kreth G, Klatte M, Lysak MA, Fuchs J, Schubert I (2004) Chromosome territory arrangement and homologous pairing in nuclei of Arabidopsis Thaliana are predominantly random except for nor-bearing chromosomes. Chromosoma 113(5):258–269. https://doi.org/10.1007/s00412-004-0316-2
Pecinka A, Kato N, Meister A, Probst A, Schubert I, Lam E (2005) Tandem repetitive transgenes and fluorescent chromatin tags alter local interphase chromosome arrangement in Arabidopsis thaliana. J Cell Sci 118:3751–3758
Pecinka A, Dinh HQ, Baubec T, Rosa M, Lettner N, Mittelsten Scheid O (2010) Epigenetic regulation of repetitive elements is attenuated by prolonged heat stress in Arabidopsis. Plant Cell 22(9):3118–3129. https://doi.org/10.1105/tpc.110.078493
Perrella G, Zioutopoulou A, Headland LR, Kaiserli E (2020) The impact of light and temperature on chromatin organisation and plant adaptation. Journal of Experimental Botany eraa154 https://doi.org/10.1093/jxb/eraa154
Pillitteri LJ, Bogenschutz NL, Torii KU (2008) The bHLH protein, MUTE, controls differentiation of stomata and the hydathode pore in Arabidopsis. Plant Cell Physiol 49(6):934–943. https://doi.org/10.1093/pcp/pcn067
Poulet A, Arganda-Carreras I, Legland D, Probst AV, Andrey P, Tatout D (2015) NucleusJ: an ImageJ plugin for quantifying 3D images of interphase nuclei. Bioinformatics 31(7):1144–1146
Poulet A, Duc C, Voisin M, Desset S, Tutois S, Vanrobays E, Benoit M, Evans DE, Probst AV, Tatout C (2017) The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants. J Cell Sci 130(3):590–601. https://doi.org/10.1242/jcs.194712
Roudier F, Ahmed I, Bérard C, Sarazin A, Mary-Huard T, Cortijo S, Bouyer D et al (2011) Integrative epigenomic mapping defines four main chromatin states in Arabidopsis. Embo J 30(10):1928–1938. https://doi.org/10.1038/emboj.2011.103
Russell WC, Newman C, Williamson DH (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253:461–462
Schmuths H, Meister A, Horres R, Bachmann K (2004) Genome size variation among accessions of Arabidopsis thaliana. Ann Bot 93(3):317–321. https://doi.org/10.1093/aob/mch037
Schubert I, Shaw P (2011) Organization and dynamics of plant interphase chromosomes. Trends Plant Sci 16:273–281
Schubert V, Berr A, Meister A (2012) Interphase chromatin organisation in Arabidopsis nuclei: constraints versus randomness. Chromosoma 121(4):369–387. https://doi.org/10.1007/s00412-012-0367-8
Schweizer D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma (berl) 58:307–324
Sequeira-Mendes J, Aragüez I, Peiró R, Mendez-Giraldez R, Zhang X, Jacobsen SE, Bastolla U, Gutierreza C (2014) The functional topography of the Arabidopsis genome is organized in a reduced number of linear motifs of chromatin states. The Plant Cell 26:2351–2366. https://doi.org/10.1105/tpc.114.124578
Snoek BL, Pavlova P, Tessadori F, Peeters AJM, Bourbousse C, Barneche F, de Jong H, Fransz PF, van Zanten M (2017) Genetic dissection of morphometric traits reveals that phytochrome B affects nucleus size and heterochromatin organization in Arabidopsis thaliana. G3:Genes, Genomes, Genetics 7(8): 2519–31 https://doi.org/10.1534/g3.117.043539
Soppe W, Jasencakova S, Houben A, Kakutani T, Meister A, Huang M, Jacobsen S, Schubert I, Fransz P (2002) DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis. EMBO J 21(23):6549–6559
Tessadori F, van Driel R, Fransz P (2004) Cytogenetics as a tool to study gene regulation. Trends Plant Sci 9(3):147–153
Tessadori F, Chupeau MC, Chupeau Y, Knip M, Germann S et al (2007a) Large-scale dissociation and sequential reassembly of pericentric heterochromatin in dedifferentiated Arabidopsis cells. J Cell Sci 120:1200–1208
Tessadori F, Schulkes RK, Driel RV, Fransz P (2007b) Light-regulated large-scale reorganization of chromatin during the floral transition in Arabidopsis. Plant J 50:848–857
Tessadori F, van Zanten M, Pavlova P, Clifoton R, Pontvianne F, Snoek B, Millenaar F, Schulkes R, van Driel R, Voesenek L, Spillane Ch, Pikaard C, Fransz P, Peeters A (2009) PHYTOCHROME B and HISTONE DEACETYLASE 6 control light-induced chromatin compaction in Arabidopsis thaliana. PLoS Genet 5(9):1–13
Traas J, Hülskamp M, Gendreau E, Höfte H (1998) Endoreduplication and development: rule without dividing? Current Opinion in Plant Biology 1:498–503. http://biomednet.com/elecref/1369526600100498
Van Zanten M, Basten Snoek L, van Eck-Stouten E, Proveniers MC, Torii KU, Voesenek LA, Peeters AJ, Millenaar FF (2010) Ethylene-induced hyponastic growth in Arabidopsis thaliana is controlled by ERECTA. Plant J 61:83–95
van Zanten M, Koini MA, Geyer R, Liu Y, Brambilla V, Bartels D, Koornneef M, Fransz P, Soppe WJJ (2011) Seed maturation in Arabidopsis thaliana is characterized by nuclear size reduction and increased chromatin condensation. PNAS 108(50):20219–20224. https://doi.org/10.1073/pnas.1117726108
Van Zanten M, Tessadori F, Peeters AJM, Fransz P (2012) Shedding light on large-scale chromatin re-organization in Arabidopsis thaliana. Mol Plant 5:57–64
Vischer NOE, Huls PG, Ghauharali RI, Brakenhoff GJ, Nanninga N, Woldringh CL (1999) Image cytometric method for quantifying the relative amount of DNA in bacterial nucleoids using Escherichia coli. J Microscopy 196(Pt 1):61–68
Williamson DH, Fennell DJ (1979) Visualization of yeast mitochondrial DNA with the fluorescent stain “DAPI.” Methods Enzymol 56:728–733. https://doi.org/10.1016/0076-6879(79)56065-0
Willemse J, Kulikova O, de Jong H, Bisseling T (2008) A new whole-mount DNA quantification method and the analysis of nuclear DNA content in the stem-cell niche of Arabidopsis roots. Plant J 55:886–894
Woo H, Richards R (2008) Natural variation in DNA methylation in ribosomal RNA genes of Arabidopsis thaliana. BMC Plant Biology 8(92). doi:https://doi.org/10.1186/1471-2229-8-92
Zalenskaja IA, Zalensky AO (2004) Non-random positioning of chromosomes in human sperm nuclei. Chromosome Res 12:163–173
Zhang X, Wessler SR (2004) Genome-wide comparative analysis of the transposable elements in the related species Arabidopsis thaliana and Brassica oleracea. PNAS 101(15):5589–5594. https://doi.org/10.1073/pnas.0401243101
Zhu D, Rosa S, Dean C (2015) Nuclear organization changes and the epigenetic silencing of FLC during vernalization. J Mol Biol 427(3):659–669. https://doi.org/10.1016/j.jmb.2014.08.025
Acknowledgements
We would like to acknowledge Dr N.O.E. Vischer and Dr J. Willemse for their support and introduction to the image processing software and Prof F. Berger for sharing plant material. Special acknowledgments to Dr F. Tessadori for heterochromatin measurements and providing data of nuclei, which we used in this article.
Funding
This research was supported by the Dutch Organization of Fundamental Research NWO-grant 050–10-020.
Author information
Authors and Affiliations
Contributions
PF and HdJ conceived and designed research. PP and PF conducted experiments. PF and BLS carried out image and statistical analytical tools. PP, PF, MvZ and BLS analyzed and interpreted data. All authors wrote (parts) of the manuscript. All authors read and approved the final version of the manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no competing interests.
Additional information
Responsible Editor: Jiming Jiang
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
The original online version of this article was revised: Due to error in the main body text and in Table 2
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Pavlova, P., van Zanten, M., Snoek, B.L. et al. 2D morphometric analysis of Arabidopsis thaliana nuclei reveals characteristic profiles of different cell types and accessions. Chromosome Res 30, 5–24 (2022). https://doi.org/10.1007/s10577-021-09673-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10577-021-09673-2