Abstract
Key message
Plant height of soybean is associated with a haplotype block on chromosome 19, which classified 211 soybean accessions into five distinct groups showing significant differences for the target trait.
Abstract
Genetic variation is pivotal for crop improvement. Natural populations are precious genetic resources. However, efficient strategies for the targeted utilization of these resources for quantitative traits, such as plant height (PH), are scarce. Being an important agronomic trait associated with soybean yield and quality, it is imperative to unravel the genetic mechanisms underlying PH in soybean. Here, a genome-wide association study (GWAS) was performed to identify single nucleotide polymorphisms (SNPs) significantly associated with PH in a natural population of 211 cultivated soybeans, which was genotyped with NJAU 355 K Soy SNP Array and evaluated across six environments. A total of 128 SNPs distributed across 17 chromosomes were found to be significantly associated with PH across six environments and a combined environment. Three significant SNPs were consistently identified in at least three environments on Chr.02 (AX-93958260), Chr.17 (AX-94154834), and Chr.19 (AX-93897200). Genomic regions of ~ 130 kb flanking these three consistent SNPs were considered as stable QTLs, which included 169 genes. Of these, 22 genes (including Dt1) were prioritized and defined as putative candidates controlling PH. The genomic region flanking 12 most significant SNPs was in strong linkage disequilibrium (LD). These SNPs formed a single haplotype block containing five haplotypes for PH, namely Hap-A, Hap-B, Hap-C, Hap-D, and Hap-E. Deployment of such superior haplotypes in breeding programs will enable development of improved soybean varieties with desirable plant height.
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Introduction
Cultivated soybean (Glycine max L.) is an important oilseed crop, grown globally as a major source of edible protein and oil (Kulkarni et al. 2016). However, in the past five decades, only a gradual increase in soybean yield has been observed in China. This can be witnessed by the fact that China imports > 80% of soybean and its products to meet its domestic requirements (Liu et al. 2018). Hence, development of soybean genotypes with desirable plant architecture and higher yield is a major objective of soybean breeders across the world, and especially from China. A significant positive correlation has been observed between PH and yield in soybean. However, taller soybean genotypes are highly prone to lodging, affecting the overall yield and seed quality (Liu et al. 2011; Lü et al. 2016). This makes PH a crucial trait in the breeding programs of soybean. Moreover, PH is a quantitative trait that is controlled by multiple loci/genes, besides being highly influenced by genotype × environment interactions (Wang et al. 2004; Cao et al. 2019). Over the years, breeding for desirable PH has been accomplished via both traditional and marker-assisted breeding (MAB) approaches (Bhat et al. 2020). In the case of environment-sensitive traits such as PH, the MAB approaches are much more effective and allow selection of plants at initial growth stages, such as germination or seedling, even for traits measured at maturity (Allen 1994; Chapman et al. 2003). Therefore, elucidating the genetic basis of PH is expected to greatly facilitate implementation of MAB in the development of high-yielding soybean varieties.
Some studies have documented the genetic basis of PH in soybean (Mansur et al. 1996; Orf et al. 1999; Liu et al. 2013; Yin et al. 2017). For instance, the past three decades have witnessed identification of > 200 quantitative trait loci (QTLs), as documented in the SoyBase (https://www.soybase.org/), and these QTLs are located across 20 soybean chromosomes. Most of the previously reported studies used linkage mapping with low-resolution markers to identify such QTLs, which often possess low genomic resolution. This has hampered successful deployment of PH QTLs in breeding programs to develop improved soybean varieties with desired PH (Cao et al. 2019). An alternative approach of trait mapping such as GWAS detects alleles/QTLs linked to the trait of interest at a higher resolution using diverse natural populations (Schmutz et al. 2010). Technological advances in next-generation sequencing-based genotyping technologies have enabled GWAS to be used routinely in crop genetics research (Song et al. 2016; Cao et al. 2019). Several studies have demonstrated the efficiency of GWAS to identify marker-trait associations (MTAs) regulating important agronomic traits and to detect the underlying putative genes in different plant species such as Arabidopsis thaliana (Ren et al. 2019), rice (Xu and Crouch 2008; Li et al. 2014), maize (Yang and Zhu 2005), wheat (Wang et al. 1999), cotton (Lai et al. 2016), soybean (Hyten et al. 2008; Cao et al. 2017; Fang et al. 2017; Li et al. 2017; Chang et al. 2018), chickpea (Varshney et al. 2019a, b; Thudi et al. 2021), pigeonpea (Varshney et al. 2017a, b), and pearl millet (Varshney et al. 2017a, b).
With the availability of whole genome sequencing data (WGS), sequencing-based trait mapping strategies have been successful in identifying QTLs/ MTAs in several crops (Varshney et al. 2019a, b). Notably, the use of WGS data has increased the mapping resolution of GWAS and enabled identification of haplotypes for the trait(s) of interest. In the post-sequencing era, ‘haplotype assembly’ has emerged as an efficient approach for the development of improved crop varieties (Bevan et al. 2017). In the past few years, haplo-pheno analysis has been successfully used for detecting superior haplotypes for agronomically important traits in some crop species. For example, Abbai et al. (2019) reported desirable haplotypes for 21 genes controlling quality and yield-related traits in rice, based on a panel of 3000 rice accessions. Similarly, superior haplotypes have been identified for drought and salinity stress adaptation in rice, soybean, and pigeonpea (Guan et al. 2014; Mishra et al. 2016; Kuroha et al. 2018; Chen et al. 2019; Sinha et al. 2020).
To understand the genetics of PH in soybean genotypes selected from major soybean growing areas of China, the present study used GWAS and haplotype analysis for identifying MTAs, candidate genes, and haplotypes. Superior haplotypes identified in this study hold potential for developing improved soybean varieties with desired plant architecture using haplotype-based breeding approach.
Material and methods
Plant material and field experiments
A natural population comprising 219 soybean germplasm accessions was evaluated for two consecutive years (2011 and 2012) at three different locations in China. This soybean germplasm represents the cultivated pool selected from different ecological regions of China, previously described by Wang et al. (2016) and Du et al. (2020) (Supplementary Table 1). The evaluated locations include the experimental fields of Nanjing Agricultural University (Nanjing); Jiangsu Yanjiang-Institute of Agricultural Sciences (Nantong); and Agricultural College of Yangzhou University (Yangzhou). Hence, the germplasm was evaluated at a total of six individual environments (location × year), namely E1–E6 (E1: Nanjing_2011, E2: Nantong_2011, E3: Yangzhou_2011, E4: Nanjing_2012, E5: Nantong_2012, and E6: Yangzhou_2012). These three locations differ substantially in temperature, precipitation, and soil quality. For example, Nanjing (32°12′ N, 118°37′ E) has a north subtropical humid climate with four distinct seasons. The average rainfall and relative humidity in Nanjing are 1106.5 mm and 76%, respectively. The annual average temperature in Nanjing is 15.4 °C, and the soil quality of Nanjing is mostly red soil with high iron content. Further, Nantong (31°58′ N, 120°53′ E) is in the alluvial plain at lower reaches of the Yangtze River with mild marine climate. The annual average temperature and precipitation in Nantong are 15.1 °C and ~ 1040 mm, respectively. There are four distinct seasons in Nantong, and the spring and autumn seasons are relatively short. The soil in Nantong is mainly yellow brown. Yangzhou (32°23′ N, 119°25′ E) is located at the southern end of the Yangtze Huaihe plain. It is affected by monsoon circulation, with four distinct seasons and a mild climate. The annual average temperature is 14.8 °C, and the average precipitation is 1020 mm. The soil texture of Yangzhou is mainly heavy soil, and a small part of it is sandy and light clay. The experiments were conducted in a complete randomized block design with three replications of each genotype. The field data for PH collected from six different environments are provided in Supplementary Table 2. Standard soybean agronomic cultural practices (Zhang et al. 2015) were followed at each location during the experiments.
Phenotypic data collection and statistical analysis
In each environment, five consecutive plants of each genotype were selected from the middle of each plot for PH phenotyping in each replication. PH was measured in centimeters (cm) by using a measuring tape stretched from the cotyledonary node to the top of the plant at the maturity stage. The data obtained from individual environments (location × year combination) and the combined environment were evaluated using “lme4” package in R environment. The analysis of variance (ANOVA) and predicted means [best linear unbiased predictions (BLUPs)] were calculated with genotype set as random. Predicted means (BLUPs) for individual environment were estimated using the model:
where Yijk is the trait (PH), µ is the overall mean effect, and Repi denotes the effect of the ith replicate/block. Genj represents the effect of the jth genotype; εij is the effect of the error associated with the ith replication/block, and jth genotype.
The ANOVA and predicted means (BLUPs) for the combined environment were estimated using the following model:
where Envi and Envi × Genk are the ith environment and the G × E interaction effects, respectively.
The broad-sense heritability for the combined and individual environment was estimated as σ2g/(σ2g + σ2ge/n + σ2ε/nr) and σ2g/(σ2g + σ2ε/r), respectively, where σg is the genetic variance, σ2ge is the interaction of genotype and environment, σ2ε is the residual error, n is the number of environments, and r is the number of replication/block (Knapp et al. 1985).
To identify the relationship between PH evaluated across different environments, we performed a Pearson’s correlation analysis. In brief, Pearson correlation analysis for soybean PH measured across different environment was conducted based on the predicted means calculated across the environment and visualized using MVApp (Julkowska et al. 2019).
Genome-wide association study
The NJAU 355 K SoySNP assay previously developed by our laboratory (Wang et al. 2016) was used in the present study. In brief, the sequencing data of 31 soybean genotypes (including 14 cultivated and 18 wild type) were used along with Williams 82 (Glyma.Wm82.a1.v1.1) as the reference genome (www.phytozome.net). The VIP variants include 60,800 SNPs from SoySNP50K15, 2103 SNPs from SoyBase, and 3671 SNPs from the sequencing data of 31 soybean genotypes. A total of 355,595 SNPs representing 609,883 probe sets were obtained following the Affymetrix filtering (Wang et al. 2016). The SNP dataset has been provided as Supplementary Table 3. In the current study, for the selection of high-quality SNPs, quality control analysis was conducted at minimum minor allele frequency (MAF) of 0.01 and missing genotype and taxa at 0.2 in TASSEL v5.2.73 (Bradbury et al. 2007).
Genome-wide association analysis was conducted with Genomic Association and Prediction Integrated Tool (GAPIT) package in R environment (Lipka et al. 2012) using a compressed mixed linear model (CMLM). This model was adopted to speed up computational time and optimize statistical performance (Lipka et al. 2012). UpSet plot was developed for significant SNPs by using the UpSetR package in R environment (Lax et al. 2014; Conway et al. 2017).
Haplotype analysis
The LD level of pairwise SNPs was calculated using Haploview 4.2 (Barnett et al. 2005). All markers associated with PH on chromosome 19 (Chr.19) within the LD ± 130 kb (Wang et al. 2016) were considered, and haplotype block was defined by “confidence intervals” algorithm (Gabriel et al. 2002). Genotypes were grouped into independent groups based on specific haplotype carried by them. To estimate the effect of haplotype on PH, groups were fitted into one-way ANOVA model in R environment as follows:
where phenotype denotes PH values in the individual and combined environments. A pairwise comparison of means was conducted using Turkey’s HSD test and visualized in R environment.
Identification of candidate genes
Within the LD decay distance of significant SNPs, all genes present within the ± 130 kb interval flanking the stable SNP positions were retrieved from SoyBase website (https://www.soybase.org/), using Glyma2.0 gene model. The functional annotations of genes were downloaded from SoyBase, and were screened manually. The RNA-seq data available at SoyBase (https://www.soybase.org/) were extracted for the genes underlying the QTL intervals from different soybean tissues (leaf, flower, pod shell, seed, root, and nodule) at different growth and developmental stages, viz., young leaf, flower, one cm pod, pod shell 10 days to flowering (DAF), pod shell 14DAF, seed 10DAF, seed 14DAF, seed 21DAF, seed 25DAF, seed 28DAF, seed 35DAF, seed 42DAF, root, and nodule) (Supplementary Table 4). These RNA-seq data were previously generated and deposited in the SoyBase by Severin et al. (2010). The RNA-seq dataset was used to analyze the expression of putative genes in different soybean tissues and across multiple development stages. For developing a heat map, the fragments per kilobase of transcript per million fragment mapped (FPKM) values of the candidate genes were used. Hierarchical clustering was performed using Euclidean Distance Method with complete linkage approach (Tao et al. 2013). A heatmap of these candidate genes was constructed using TBtools_JRE1.6 software (Chen et al. 2020). Based on functional annotations of genes, available literature, and gene expression patterns, some genes were defined as putative candidates regulating PH.
Results
Phenotypic characterization and correlation analysis of PH in soybean accessions
The descriptive statistics including mean, range (maximum and minimum values), standard deviation, skewness, kurtosis, broad-sense heritability, and coefficient of variation (CV) for PH in 219 soybean accessions evaluated across six different environments and the combined environment are presented in Table 1. In brief, the PH ranged from 8.24 cm in E6 to 311.67 cm in E4. The mean across individual environments ranged from 52.76 ± 0.83 cm in E3 to 105.30 ± 1.73 in E4. The CV in the combined environment was 49%, and ranged from 38% in E6 to 49.8% in E2. Furthermore, skewness and kurtosis were 1.34 and 2.84, respectively in the combined environment (Table 1). The broad-sense heritability ranged from 0.84 in E6 to 0.98 in E4, and 0.97 in the combined environment. The soybean accessions (G), environment (E), and the genotype × environment interaction (G × E) had significant effect (P < 0.0001) on PH (Table 2). Moreover, Pearson correlation analysis of PH showed a significantly positive correlation (P < 0.0001) across different environments evaluated (Supplementary Table 5).
Genome-wide distribution of SNPs in soybean natural population
In the present study, a total of 211 soybean accessions and 291,962 SNP markers were retained after quality control analysis (Table 3). These markers covered 949,992,372 bp (949.99 Mb) of the soybean genome, representing ~ 85% of the genome. The number of SNPs in each chromosome varied from 12,035 to 19,880, with the lowest number of SNPs present on Chr.11, whereas Chr.18 contained the highest number of SNPs. The lowest and highest SNP density of 264.27 SNPs/Mb and 361.97 SNPs/Mb was found on Chr.01 and Chr.13, respectively (Supplementary Fig. 1; Table 3).
GWAS revealed genetic architecture of PH in soybean
A total of 128 SNPs were found to be significantly associated with PH across six environments and the combined environment, based on GWAS results (Fig. 1; Supplementary Table 6). The distribution of markers across soybean chromosomes and their intersections based on the individual and combined environments are presented in Fig. 2 as UpSet plot (Lex et al. 2014). The UpSet plots provides a convenient alternative to visualize the data sets by frequency. In Fig. 2a, the horizontal bar graph on the left-hand side of the UpSet plot showed the size of each set, i.e., total number of significant SNPs identified in each individual environment and the combined environment. For example, the horizontal bar linked to E5 environment indicated the presence of 58 significant SNPs identified in this environment. Similarly, the horizontal bars corresponding to other five individual environments (E1, E2, E3, E4, and E6) and the combined environment showed the number of significant SNPs identified in each of these environments (Fig. 2a). Furthermore, the vertical bars on the top indicate the individual set participation sizes (Fig. 2a). For instance, the vertical bar on the left most side shows that out of the 58 significant SNPs identified in E5 environment, 49 SNPs were specific for E5 environment, two SNPs were detected in E4 and E5, one SNP in E2, E4, and E5; one SNP in E2, E3, and E5; four SNPs in E1, E3, E5, E6, and CE; and one SNP was detected in E1, E2, E3, E4, E5, E6, and CE (Fig. 2a). In Fig. 2b, each vertical bar reveals the number of significant SNPs associated with PH identified on each chromosome. Notably, three significant SNPs were consistently identified in three or more environments (Fig. 2; Table 4). These included a SNP (AX-93897200) on Chr.19, which was detected in all six environments and the combined environment, a SNP (AX-93958260) on Chr.02 and a SNP (AX-94154834) on Chr.17 that were detected in three different environments. The regions within ~ 130 kb interval [based on the LD decay previously reported for the same population by using NJAU 355 K SoySNP assay (Wang et al. 2016)] flanking the significant SNPs (AX-93958260, AX-94154834, and AX-93897200) present on Chr.02, Chr.17, and Chr.19, were referred as qPH2, qPH17 and qPH19, respectively. These QTLs/genomic regions represented stable genetic elements regulating PH in soybean.
Superior haplotypes for PH identified on soybean Chr. 19
The genomic region flanking three stable significant SNPs (AX-93958260, AX-94154834, and AX-93897200) within the LD decay of ± 130 kb was subjected to haplotype analysis. The results showed that only significant markers identified within the LD decay flanking the SNP AX-93897200 (detected in all six environments and the combined environment) on Chr.19 formed a haplotype block. No haplotype block was detected for other two significant SNPs within the referenced LD decay (± 130 kb) on Chr.02 and Chr.17. Hence, the SNP (AX-93897200) along with 11 other SNPs (AX-94195032, AX-93897192, AX-93659063, AX-94195035, AX-93897195, AX-93897196, AX-93955268, AX-93897197, AX-93897198, AX-93897199, and AX-93897201) were found to be in strong LD and formed a single haplotype block (Fig. 3). The length of this haplotype block was less than 0.15 Mb. These 12 SNPs within the haplotype block represented five haplotypes across 211 accessions of the soybean natural population. Notably, these haplotypes classified the genotypes of the studied soybean population into five groups. The PH of the genotypes varied significantly across the five haplotype groups. For instance, Haplotype-A (Hap-A) formed the largest group (n = 102), followed by Hap-B (n = 72), Hap-C (n = 16), Hap-D (n = 17), and Hap-E (n = 04) (Fig. 3c). The nucleotide variation detected for these haplotypes is represented in Supplementary Fig. 2.
The differences in PH based on different haplotype alleles identified within the haplotype block on Chr.19 in soybean GWAS population varied across environments. However, soybean accessions with Hap-D appeared to be the tallest across environments, those with Hap-E and Hap-B had a dwarf phenotype, and accessions with Hap-A and Hap-C were of intermediate height (Fig. 3c; Supplementary Fig. 3).
Identification of candidate genes within three QTL intervals
A total of 169 genes were identified within the physical genomic interval of all three major QTLs (qPH2, qPH17 and qPH19), which consisted of 18, 35, and 116 genes, respectively (Supplementary Table 7). Of the 169 genes detected, the RNA-seq data for only 124 genes were available online at SoyBase (https://www.soybase.org/) for 14 soybean tissues across different stages of growth and development (Supplementary Table 4). Expression pattern of the genes obtained from the RNA-seq data is represented in the form of a heatmap in Fig. 4. Furthermore, based on in silico analysis of gene expression data and gene annotations, we defined a total of 22 candidate genes underlying three stable genomic regions. This includes two genes underlying qPH2, five genes underlying qPH17, and 15 genes underlying qPH19 (Table 5). The selection of these candidate genes was based on gene function annotation (i.e., genes regulating cell division, meristem growth, plant growth hormone biosynthesis and signaling, cell elongation, and transition from vegetative to reproductive phase), literature studies, and gene expression profiles (Table 5; Supplementary Table 4). These 22 genes can be considered as putative candidates controlling PH. However, further functional validation of these genes is needed to determine their exact role in regulating PH in soybean.
Discussion
Plant height is an important determinant of soybean yield and quality (Lu et al. 2016; Yin et al. 2017). Taller soybean cultivars/varieties are often subjected to lodging, which leads to considerable reduction in soybean production as well as deterioration of its quality (Cao et al. 2019). Hence, tailoring PH in soybean to avoid qualitative and quantitative losses has been a long-term goal of soybean breeders. Determining the genetic basis of PH is a prerequisite to develop soybean varieties with desirable plant architecture. Studies have documented the complex inheritance of PH, besides the trait being strongly influenced by G × E interactions (Lee et al. 2015). Therefore, the present study was undertaken to identify the genetic loci/QTLs, and superior haplotypes regulating PH in soybean by using GWAS and haplotype analysis.
In the present study, ANOVA results revealed a significant difference among 211 accessions for PH (P < 0.01). In accordance with previous studies (Zhang et al. 2004; Cao et al. 2019), our study confirmed that PH is significantly affected by G, E, and G × E interactions. This suggests the complex genetic nature of PH in soybean. Furthermore, the estimate of broad-sense heritability for PH was high (98.95%) in the soybean natural population across all six environments, which is consistent with previous studies (Cao et al. 2019). Higher broad-sense heritability reported for PH in this study suggests a high probability of achieving the same phenotypic performance for PH when grown in the same environment. In agreement with our findings, many authors have earlier reported similar results for PH in the soybean germplasm collection (Zhang et al. 2015; Chang et al. 2018; Xue et al. 2019).
To date, the genetic basis of PH in soybean has been documented by multiple research groups (Orf et al. 1999; Chapman et al. 2003; Liu et al. 2013; Yin et al. 2017; Cao et al. 2019). However, the majority of previously detected QTLs have not been validated, which limit their deployment in MAB efforts. To this end, a more powerful and high-resolution mapping approach called the LD-based GWAS was used in the present study. Previous studies have demonstrated that GWAS is an effective strategy to determine the genetic basis of complex crop traits, such as PH, in a more precise and accurate manner (Kim et al. 2012; Cober and Morrison 2010). Hence, in the present study, we used GWAS for the identification of genomic regions associated with PH in soybean. We identified a total of 128 SNPs linked with PH across all the environments, which were found to be distributed on all chromosomes except Chr.06, Chr.18, and Chr.20. These results suggest the genome-wide distribution of loci/genes regulating PH in soybean and reveal the complex genetic architecture of this trait. In agreement with our results, Zhang et al. (2015) reported the distribution of significant MTAs for PH on 15 different chromosomes of soybean. Similar results have also been reported by other researchers (Fang et al. 2017, 2020; Chang et al. 2018). Further, the number of significant SNPs linked to PH was found to vary considerably across six different environments. For example, the highest number of SNPs were identified in E5, whereas the lowest number of SNPs were detected in E2. These findings suggest some effect of environment on the regulation of PH in soybean, which is in accordance with previous reports (Xue et al. 2019; Fang et al. 2020).
Importantly, three significant SNPs were consistently identified across multiple environments on Chr.02, Chr.17, and Chr.19. The genomic regions (~ 130 kb) flanking significant SNPs were referred as QTLs related to PH based on the LD decay. These QTLs/genomic regions represent stable genetic elements regulating PH in soybean. The QTL on Chr.02 (Plant height 26–9) associated with PH has been previously reported in the genomic region between 15,993,654 and 41,032,570 bp by Sun et al. (2006), and the genomic region underlying qPH2 was found to lie in the same physical interval. Therefore, qPH2 might be the same as Plant height 26–9, as earlier reported by Sun et al. (2006). However, when compared to Plant height 26–9, the physical interval of qPH2 was considerably narrowed down in the present study. Furthermore, qPH19 identified in the present study was found to co-locate with two previously identified QTLs viz., Plant height 6–1 (44,862,405–45,270,324 bp) and Plant height 10–4 (44,862,405- 45,270,324 bp) on Chr.19 (Lark et al. 1995; Orf et al. 1999). However, there is no QTL identified to date that falls within the physical interval of qPH17. Hence, qPH17 can be regarded as a novel QTL identified in the current study. The physical intervals of qPH2 and qPH19 were considerably reduced in the present study. This may be because the earlier reported QTLs (Plant height 26–9, Plant height 6–1, and Plant height 10–4) were identified via linkage mapping by using low-density markers such as simple sequence repeat and restriction fragment length polymorphism, possessing a relatively low genomic resolution. These marker systems have low selection accuracy, which has been the biggest obstacle for their deployment in the MAB programs. QTL mapping involves the use of bi-parental mapping populations, which are associated with poor resolution in the detection of target loci regulating traits of interest (Kraakman et al. 2004). To this end, GWAS has emerged as a powerful approach using the ancestral recombination events and has high-resolution in the detection of MTAs. Therefore, high resolution of GWAS in the identification of the stable QTLs across multiple environments will facilitate their effective utilization in MAB programs for breeding soybean varieties with desirable PH.
In the practical breeding programs, the identification of potential markers/candidate genes for the trait of interest underlying a particular genomic region is the ultimate objective (Ganie and Ahammed 2021; Ganie et al. 2021). To date, minimal efforts have been made to identify candidate genes regulating PH in soybean (Liu et al. 2013; Lee et al. 2015; Cao et al. 2017), and only few genes have been characterized (Liu et al. 2010; Ping et al. 2014; Zhang et al. 2018). Hence, based on literature research and gene annotations, we defined 22 candidate genes underlying qPH2, qPH17, and qPH19 that may possibly regulate PH. For example, Dt1 (Glyma.19G194300) gene lying within the physical interval of qPH19 was considered as the candidate gene. The same locus on Chr.19 underlying the Dt1 locus was also identified previously (Sonah et al. 2015; Kato et al. 2019). Liu et al. (2010) observed regulation of the growth habits and PH in soybean by Dt1. In addition to the growth habit genes, maturity and flowering time genes also have a major impact on PH (Cober and Morrison 2010; Xia et al. 2012; Zhang et al. 2015; Cao et al. 2017). Gene function related to growth hormones, mitosis, cell division, cell elongation or functions that are directly or indirectly related to the vegetative growth are involved in regulating PH. To this end, a total of 169 gene models were found underlying the physical intervals of three stable QTLs, and 22 genes (including Dt1) were defined as putative candidates controlling PH by taking into consideration their annotations and gene expression patterns. However, further verification and functional validation of these genes are required to precisely pinpoint their role in the regulation of PH in soybean.
Recently, haplotype-based breeding has emerged as a promising approach to develop custom-designed crop varieties (Varshney et al. 2021). However, this breeding approach needs detection of superior haplotypes for their ultimate use in breeding programs. Haplotype analysis allows plant breeders to utilize the genetic variation underlying key genes/loci to the greatest extent. For example, in soybean, a candidate gene regulating salinity tolerance (GmCHX1) was subjected to haplotype analysis. Among various haplotypes identified for GmCHX1, SV-2 was found to provide maximum salinity tolerance (Patil et al. 2016). Moreover, Wang et al. (2017) identified superior haplotypes for grain quality traits (cooking and eating quality traits) in rice. Sinha et al. (2020) performed haplotype analysis of five genes controlling drought tolerance in pigeonpea. In a given genomic region, many factors regulate haplotype variation, such as mutation, recombination rates, and selection (Zaitlen et al. 2005). Hence, it is a prerequisite to include heterozygous haplotypes to capture maximum haplotypic variation for the analysis of haplotype diversity (Sinha et al. 2020). In our study, the genomic region flanking the most stable significant SNP (AX-93897200) that was in strong LD with 11 significant SNPs formed a single haplotype block. Five haplotypes (Hap-A, Hap-B, Hap-C, Hap-D, and Hap-E) were detected in this haplotype block. The results showed that five haplotypes identified in the block on Chr.19 regulate PH in soybean from dwarf ➝ intermediate ➝ tall. However, our results also reported that these haplotype alleles showed significant environment interaction across six different environments in the regulation of PH. This suggests the presence of haplotype × environment interaction, which needs to be fixed before the utilization of superior haplotype(s) in crop improvement.
A previous study has documented only two haplotypes underlying the Dt1 locus viz., Gm19_Hap42b and Gm19_Hap42a regulating two different phenotypes of PH (Contreras-Soto et al. 2017). This haplotype block showed only two haplotypes (biallelic), and the significant SNP within this region was also biallelic. Therefore, this study showed no difference between the uses of the haplotype or SNP markers within the target region for MAB. The main objective behind the deployment of haplotype markers is that they are multi-allelic and thus provide better opportunity to modify the plant trait. For example, in the present study we identified five different haplotypes for PH underlying the Dt1 locus viz., Hap-A, Hap-B, Hap-C, Hap-D, and Hap-E which regulated five different phenotype classes of PH in soybean. This provides a greater possibility to modify PH in soybean. Therefore, the incorporation of these haplotypes via haplotype-based breeding in soybean breeding programs will be very useful to tailor plant architecture and improve yield and quality.
Conclusion
The present study identified 128 significant SNPs and three stable QTLs/genomic regions (qPH2, qPH17, and qPH19) associated with PH, which were consistently detected in at least three environments. Out of these three QTLs, the QTLs co-localizing with qPH2 and qPH19 have been previously detected by other researchers. However, no QTL was found to be associated with qPH17, implying this locus to be a novel genomic region regulating PH. A total of 22 candidate genes underlying these three QTL regions were also prioritized. The putative candidates defined within the intervals of qPH2, qPH17, and qPH19 need further functional validation before they can be used in soybean breeding programs. The haplotype analysis detected five haplotypes (Hap-A, Hap-B, Hap-C, Hap-D, and Hap-E) within the stable genomic region of Chr.19, which regulated PH ranging from dwarf ➝ intermediate ➝ tall. The superior haplotypes identified in the Chinese soybean germplasm will serve as a potential resource for haplotype-based breeding of PH in soybean.
Data availability
All relevant data can be found within the manuscript and in Supplementary Material.
Code availability
The scripts used for performing different analysis in the present study are provided in Supplementary File 1.
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Open Access funding enabled and organized by CAUL and its Member Institutions. This work was supported in part by National Natural Science Foundation of China (No. 32090065), Ministry of Science and Technology (No. 2017YFE0111000), and Horizon 2020 of European Union (EUCLEG/ No. 727312).
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JAB, DY, and RKV conceived and planned the experiments. JAB and DH performed the experiments. KAA, JAB, BK, SAG, RB, and DH analyzed the data. KAA, JAB, BK, and SAG wrote the initial draft of the manuscript. JAB, DY, and RKV supervised the project. All the authors read and revised the final manuscript.
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Bhat, J.A., Karikari, B., Adeboye, K.A. et al. Identification of superior haplotypes in a diverse natural population for breeding desirable plant height in soybean. Theor Appl Genet 135, 2407–2422 (2022). https://doi.org/10.1007/s00122-022-04120-0
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DOI: https://doi.org/10.1007/s00122-022-04120-0