Abstract
The Chinese alligator, Alligator sinensis, is a critically endangered species. A conservation project of gene resources for an endangered species first involves the preservation of organs, tissues, gametes, genomic DNA libraries and cell lines. The present study is the first to establish and cryopreserve cell lines of liver, heart and muscle tissues from the Chinese alligator. The study revealed that there was a large discrepancy in cell migration time in primary cultures among liver (11–12 d), heart (13–14 d) and muscle (17–18 d) tissue pieces. The differences in time in primary cell culture suggested that it was relatively easy to build visceral-derived cell lines for reptiles. Biological analysis showed that the population doubling time for thawed cells was approximately 36 h. Karyotyping revealed that the frequency of Chinese alligator cells showing chromosome number as 2n=32 was 88.6%–93.4%. Chinese alligator cell lines established here provide a vital resource for research and are likely to be useful for protection of this rare and critically endangered species. Furthermore, the establishment of these methods may supply technical and theoretical support for preserving genetic resources at the cellular level for other reptile species.
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References
Xu Q H, Fang S G, Wang Z W, et al. Heavy metal distribution in tissues and eggs of Chinese alligator (Alligator sinensis). Arch Environ Contam Toxicol, 2006, 50: 580–586
Thorbjarnarson J, Wang X M, Ming S, et al. Wild populations of the Chinese alligator approach extinction. Biol Conserv, 2002, 103: 93–102
Ding Y Z, Wang X M. Factors influencing the population status of wild Chinese alligators (Alligator sinensis). Biodivers Sci, 2004, 12: 324–332
Chen B C. The past and present situation of the Chinese alligator. As Herp Res, 1990, 3: 129–136
Wan Z, Gu C, Wang X, et al. Conservation, management and farming of crocodiles in China. In: Crocodiles. Proceedings of the 14th Working Meeting of the Crocodile Specialist Group. IUCN-The World Conservation Union, Gland, Switzerland and Cambridge, UK, 1998. 80–100
Ross J P. Crocodiles: Status Survey and Conservation Action Plan. 2nd ed. IUCN/SSC Crocodile Specialist Group, IUCN, Gland, Switzerland and Cambridge, UK, 1998
Valleley E M, Harrison C J, Cook Y, et al. The karyotype of Alligator mississippiensis, and chromosomal mapping of the ZFY/X homologue, Zfc. Chromosoma, 1994, 103: 502–507
Guan W, He X, Li L, et al. Establishment and biological characterization of fibroblast cell line from the Langshan chicken. Cell Prolif, 2010, 43: 157–163
Gu Y P, Li H Z, Mik J. Phenotypic characterization of telomerase-immortalized primary non-malignant and malignant tumor-derived human prostate epithelial cell lines. Exp Cell Res, 2006, 312: 841–843
Kong D, Nishino N, Shibusawa M, et al. Establishment and characterization of human pancreatic adenocarcinoma cell line in tissue culture and the nude mouse. Tissue Cell, 2007, 39: 217–223
Lui J F, Valencia E F T, Boer J A. Karyotypic and analysis and chromosome biometry of cell cultures of the yellow throated alligator (caiman latirostris Daudin). Rev Brasil Genet, 1994, 17: 165–169
Li L F, Yue H, Ma J, et al. Establishment and characterization of a fibroblast line from Simmental cattle. Cryobiology, 2009, 59: 63–68
Xue Q S. The Principle and Technique of in vitro Culture. Beijing: Science Press, 2001. 432–444
Ferguson M W, Joanen T. Temperature of egg incubation determines sex in Alligator mississippiensis. Nature, 1982, 296: 850–853
Wilber C G. Effect of temperature on the heart in the alligator. Am J Physiol, 1960, 198: 861–863
Guan W J, Liu C Q, Li C Y, et al. Establishment and cryopreservation of a fibroblast cell line derived from Bengal tiger (Panthera tigris tigris). Cryo Letters, 2010, 31: 130–138
King M, Honeycutt R, Contreras N. Chromosomal repatterning in crocodiles: C, G and N-banding and the in situ hybridization of 18S and 26S rRNA cistrons. Genetica, 1986, 70: 191–201
Cohen M M, Gans C. The chromosomes of the order Crocodilia. Cytogenetics, 1970, 9: 81–105
Fishman H K, Mitra J, Dowling H G. The karyotype of the Chinese alligator (Alligator sinensis). Mammal Chrom Newsl, 1968, 9: 81–82
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Zeng, C., Ye, Q. & Fang, S. Establishment and cryopreservation of liver, heart and muscle cell lines derived from the Chinese alligator (Alligator sinensis). Chin. Sci. Bull. 56, 2576–2579 (2011). https://doi.org/10.1007/s11434-011-4622-9
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DOI: https://doi.org/10.1007/s11434-011-4622-9