Abstract
Myofibrillogenesis regulator-1 (MR-1) is a gene overexpressed usually in many human cancers. However, the effects of MR-1 on cell proliferation, adhesion, migration and genome-wide gene regulation are still unclear. In this study, a human hepatoma cell line that highly overexpresses MR-1, BEL-7402/MR-1 cells was established. While the high expression of MR-1 did not promote cell proliferation, it significantly increased cell spreading, adhesion and migration compared with control cells. A total of 147 genes were regulated by MR-1 expression, 46 genes were down-regulated and 101 genes were up-regulated by MR-1 overexpression. Many of these genes were related to cell adhesion, cytoskeletal regulation, MAPK signaling, and cell cycle related pathways. Western blot analysis further confirmed the regulation of pathways associated with migration by MR-1. These results suggest that MR-1 is involved in the regulation of cancer cell adhesion, migration and related gene expression.
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Chambers A F, Groom A C. MacDonald I C. Dissemination and growth of cancer cells in metastatic sites. Nat Rev Cancer, 2002, 2: 563–572
Fidler I J. The pathogenesis of cancer metastasis: The “seed and soil” hypothesis revisited. Nat Rev Cancer, 2003, 3: 453–458
Gupta G P, Massague J. Cancer metastasis: Building a framework. Cell, 2006, 127: 679–695
Bernards R, Weinberg R A. A progression puzzle. Nature, 2002, 418: 823
Wang Y G, Li T B, Feng S. Cell reconstruction, transduction and apoptosis related gene CR1. PRC Patent, CN 02153656.2, 2002
Li T B, Liu X H, Feng S, et al. Characterization of MR-1, a novel myofibrillogenesis regulator in human muscle. Acta Biochim Biophys Sin (Shanghai), 2004, 36: 412–418
Lu R, Sun M, Feng J, et al. Myofibrillogenesis regulator 1 (MR-1) is a novel biomarker and potential therapeutic target for human ovarian cancer. BMC Cancer, 2011, 11: 270
Ren K, Jin H, Bian C, et al. MR-1 modulates proliferation and migration of human hepatoma HepG2 cells through myosin light chains-2 (MLC2)/focal adhesion kinase (FAK)/Akt signaling pathway. J Biol Chem, 2008, 283: 35598–35605
Li H L, She Z G, Li T B, et al. Overexpression of myofibrillogenesis regulator-1 aggravates cardiac hypertrophy induced by angiotensin II in mice. Hypertension, 2007, 49: 1399–1408
Wang J H, Bian C J, Zhao C H, et al. MR1 siRNA suppresses proliferation of human hepatoma BEL-7402 cells (in Chinese). Basic Clinical Medicine, 2009, 29: 1–5
Zhao M, He H W, Sun H X. Dual knockdown of N-ras and epiregulin synergistically suppressed the growth of human hepatoma cells. Biochem Biophys Res Commun, 2009, 387: 239–244
Huang D W, Sherman B T, Lempicki R A. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc, 2009, 4: 44–57
Pearson G W, Hunter T. Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells. J Cell Biol, 2007, 179: 1555–1567
Yamazaki D, Kurisu S, Takenawa T. Regulation of cancer cell motility through actin reorganization. Cancer Sci, 2005, 96: 379–386
Nobes C D, Hall A. Rho GTPases control polarity, protrusion, and adhesion during cell movement. J Cell Biol, 1999, 144: 1235–1244
Alblas J, Ulfman L, Hordijk P. Activation of Rhoa and ROCK are essential for detachment of migrating leukocytes. Mol Biol Cell, 2001, 12: 2137–2145
Raftopoulou M, Hall A. Cell migration: Rho GTPases lead the way. Dev Biol, 2004. 265: 23–32
Li D, Ding J, Wang X, et al. Fibronectin promotes tyrosine phosphorylation of paxillin and cell invasiveness in the gastric cancer cell line AGS. Tumori, 2009, 95: 769–779
Griffith O L, Melck A, Jones S J, et al. Meta-analysis and metareview of thyroid cancer gene expression profiling studies identifies important diagnostic biomarkers. J Clin Oncol, 2006, 24: 5043–5051
Thalappilly S, Soubeyran P, Iovanna J L, et al. VAV2 regulates epidermal growth factor receptor endocytosis and degradation. Oncogene, 2010, 29: 2528–2539
Gu J, Isaji T, Sato Y, et al. Importance of N-glycosylation on alpha5beta1 integrin for its biological functions. Biol Pharm Bull, 2009, 32: 780–785
Choi Y, Kim H, Chung H, et al. Syndecan-2 regulates cell migration in colon cancer cells through Tiam1-mediated Rac activation. Biochem Biophys Res Commun, 2010, 391: 921–925
Laurila E, Savinainen K, Kuuselo R, et al. Characterization of the 7q21–q22 amplicon identifies ARPC1A, a subunit of the Arp2/3 complex, as a regulator of cell migration and invasion in pancreatic cancer. Genes Chromosomes Cancer, 2009, 48: 330–339
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Zhao, W., He, H., Ren, K. et al. Myofibrillogenesis regulator-1 promotes cell adhesion and migration in human hepatoma cells. Chin. Sci. Bull. 58, 3007–3014 (2013). https://doi.org/10.1007/s11434-013-5933-9
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DOI: https://doi.org/10.1007/s11434-013-5933-9