Abstract
Background
Cryptosporidium is second only to rotavirus as a cause of moderate-to-severe diarrhea in young children. There are currently no fully effective drug treatments or vaccines for cryptosporidiosis. MicroRNAs (miRNAs) are involved in regulating the innate immune response to Cryptosporidium parvum infection. In this study, we investigated the role and mechanism of miR-3976 in regulating HCT-8 cell apoptosis induced by C. parvum infection.
Methods
Expression levels of miR-3976 and C. parvum burden were estimated using real-time quantitative polymerase chain reaction (RT-qPCR) and cell apoptosis was detected by flow cytometry. The interaction between miR-3976 and B-cell lymphoma 2-related protein A1 (BCL2A1) was studied by luciferase reporter assay, RT-qPCR, and western blotting.
Results
Expression levels of miR-3976 were decreased at 8 and 12 h post-infection (hpi) but increased at 24 and 48 hpi. Upregulation of miR-3976 promoted cell apoptosis and inhibited the parasite burden in HCT-8 cells after C. parvum infection. Luciferase reporter assay indicated that BCL2A1 was a target gene of miR-3976. Co-transfection with miR-3976 and a BCL2A1 overexpression vector revealed that miR-3976 targeted BCL2A1 and suppressed cell apoptosis and promoted the parasite burden in HCT-8 cells.
Conclusions
The present data indicated that miR-3976 regulated cell apoptosis and parasite burden in HCT-8 cells by targeting BCL2A1 following C. parvum infection. Future study should determine the role of miR-3976 in hosts’ anti-C. parvum immunity in vivo.
Graphical Abstract
Similar content being viewed by others
Background
Cryptosporidium parvum is a zoonotic apicomplexan protozoon and a causal pathogen of cryptosporidiosis [1]. The development of cryptosporidiosis depends largely on the immune status of the infected individual; self-limited diarrhea is the main symptom in immunocompetent individuals, but infection can lead to persistent diarrhea and death in immunocompromised individuals, especially AIDS patients [2,3,4]. Cryptosporidium is the second-leading cause of diarrhea-associated mortality in children under 5 years of age, and was responsible for 604,000 deaths in 2015 [5, 6]. Cryptosporidium parvum has been recognized as one of the most common and dangerous opportunistic parasites causing diarrhea, and a significant association has been demonstrated between C. parvum and malnutrition, including growth failure and wasting, in children in developing countries [7,8,9,10]. There are currently no effective drug treatments or vaccines for cryptosporidiosis. Nitazoxanide is the only approved drug for cryptosporidiosis, but its efficacy is limited in immunocompetent individuals and it is not effective in immunocompromised individuals, especially HIV/AIDS patients [11]. There is thus an urgent need to develop new drugs and/or vaccines to treat cryptosporidiosis. However, the pathogenic mechanism of cryptosporidiosis remains largely unknown, and this lack of information limits the availability of effective treatment and prevention strategies.
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level by pairing to the 3′-untranslated region (3′-UTR) of target messenger RNAs (mRNAs), either perfectly or imperfectly [12]. This interaction regulates a series of cellular processes including cell proliferation, apoptosis, and differentiation [13, 14]. Because C. parvum lacks the key components required for small RNA-mediated post-transcriptional gene silencing, it is an ideal model for investigating host miRNA-mediated defenses against infection in epithelial cells [15]. Increasing evidence has demonstrated that miRNAs may modulate the host epithelial immune responses following C. parvum infection [16]. For example, C. parvum infection induced the expression of let-7i and miR-513, which contributed to cholangiocyte immune responses against C. parvum infection in vitro [17,18,19]. Some evidence also suggested that C. parvum utilized host miRNAs to escape the host defense and promote its own survival [19, 20]; for example, transcriptional activation of miR-27b targeted KH-type splicing regulatory protein (KSRP) to regulate the burden of C. parvum infection [20]. Studying the mechanism by which miRNAs regulate epithelial immunity against C. parvum infection will further our understanding of the interaction between C. parvum and its host, and aid the screening of potential drug targets.
Apoptosis is considered to be an important defense mechanism against Cryptosporidium infection during innate immunity [21]. Cryptosporidium parvum-induced apoptosis in infected and uninfected biliary epithelial cells via a factor-associated suicide (Fas)/Fas ligand (FasL)-dependent mechanism, which disturbs parasite propagation and facilitates clearance [22]. However, although studies on miRNA expression profiles have demonstrated the inductive potential of C. parvum with respect to apoptosis [23], little is known about the regulation of host cell apoptosis by miRNAs following Cryptosporidium infection. We previously examined the miRNA expression profiles of HCT-8 cells infected with C. parvum and found that miR-3976, miR-942-5p, miR-181d, miR-18b-3p, miR-34b-5p, and miR-3591-3p regulated apoptosis during the early phase of infection [23]. The present study aimed to clarify the mechanism by which miR-3976 regulates HCT-8 cell apoptosis following C. parvum infection.
Methods
Cell culture and C. parvum infection model
The human ileocecal adenocarcinoma (HCT-8) cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 4 mmol/l l-glutamine, 100 U/ml penicillin, and 100 U/ml streptomycin at 37 °C in 5% CO2. Neonatal calves were purchased from Ruiya Animal Husbandry Co., Ltd. (Zhengzhou, China). Cryptosporidium parvum subtype IIdA19G1 oocysts were passaged and maintained in neonatal calves and stored in 2.5% K2Cr2O7 solution at 4 °C after purification. The oocysts used in the experiment have been passaged for about 20 generations. Oocysts were excysted in 0.25% trypsin and 0.75% sodium taurocholate for 1 h, with mixing every 5 min, followed by incubation at room temperature for 30 min, as described previously [24], followed by washing three times in phosphate-buffered saline (PBS) and resuspension. Monolayer cells in 6- or 12-well plates were inoculated with sporozoites at a ratio of 5:1 sporozoites to cells.
Real-time quantitative polymerase chain reaction (RT-qPCR)
HCT-8 cells were washed three times with PBS and total RNA was isolated using TRIzol reagent (Invitrogen, Waltham, MA, USA) following the kit manufacturer’s instructions, and subsequent to treatment with Recombinant DNase I (Takara, Kyoto, Japan). Extracted RNA (1 μg) was reverse transcribed into complementary DNA (cDNA) and miRNA using ReverTra Ace qPCR RT Master Mix with gDNA [genomic DNA] Remover (Toyobo, Japan) and a Mir-X miRNA First-Strand Synthesis Kit (Takara Biomedical Technology, Dalian, China) following the manufacturer’s protocols. Housekeeping genes gapdh (glyceraldehyde-3-phosphate dehydrogenase) and u6 were used as baselines for cDNA and miRNA quantification. cDNA or miRNA expression were determined using SYBR® Green Realtime PCR Master Mix (Toyobo). RT-qPCR was performed with melting curve analysis under the following the cycling program: initial activation at 95 °C for 30 s, followed by 40 cycles of denaturation at 95 °C for 5 s, annealing at 55 °C for 10 s, and extension at 72 °C for 15 s. Fold-change differences in gene expression were calculated using the 2−ΔΔCt method to show the relative expression of each RNA. The change in gene expression in treated groups compared to the control group was shown on the Y-axis. The primer sequences used in the present study are listed in Additional file 1: Table S1.
Cell transfection
MiR-3976 mimics, inhibitors, and negative controls (NCs) were obtained from GenePharma (Shanghai, China). The B-cell lymphoma 2-related protein A1 (BCL2A1) overexpression vector was constructed using the pcDNA3.1 eukaryotic expression plasmid (pcDNA3.1-BCL2A1); the overexpression plasmid map is presented in Additional file 4: Fig. S1. Small interfering RNAs (SiRNAs) targeting BCL2A1 mRNAs (si-BCL2A1) and scrambled RNA (si-NC) were designed by Sangon Biotech (Shanghai, China). Transfection was carried out using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s protocols. MiR-3976 mimics and pcDNA3.1-BCL2A1 were co-transfected with an equal quantity. The sequences of the siRNA and miRNA mimics and inhibitors are presented in Additional file 2: Table S2.
Flow cytometry for cell apoptosis
Cells were washed with PBS at 48 hpi, digested with ethylenediaminetetraacetic acid (EDTA)-free pancreatic enzymes for 5 min, and centrifuged at 800×g for 5 min at room temperature. The centrifuged supernatant was removed and the cells were washed twice with PBS. Using a PE Annexin V Apoptosis Detection Kit I (BD, Denver, CO, USA), cells were resuspended with 500 µl of precooling 1× binding buffer to achieve cell density of 105–106/ml, followed by the addition of 5 µl of Annexin V-PE and 5 µl of 7-aminoactinomycin D. The resuspension was incubated for 15 min in the dark at room temperature. Cell apoptosis was detected using flow cytometry (Beckman Coulter, Brea, CA, USA).
Dual luciferase assay
The sequences of wild-type (WT) BCL2A1 3′-UTR (BCL2A1-WT) and mutant BCL2A1 3′-UTR (BCL2A1-MUT) were designed and inserted into pmirGLO vectors (Promega, Madison, WI, USA). Sequences of BCL2A1-WT 3′-UTR and BCL2A1-MUT 3′-UTR are listed in Additional file 3: Table S3 and the sequences of pmirGLO-BCL2A1-WT and pmirGLO-BCL2A1-MUT are presented in Additional file 5. Each constructed plasmid was co-transfected into HCT-8 cells with miR-3976 mimics or NC using Lipofectamine 2000 reagent (Invitrogen, Gaithersburg, MD, USA). The luciferase activity of the transfected cells was measured after 48 h using the Dual-GLO® Luciferase Assay System (Promega, Madison, WI, USA). Relative luciferase activity was finally calculated by firefly luciferase value/Renilla luciferase value and normalized to the activity of the co-transfected Renilla luciferase.
Western blotting
Total proteins were extracted from 107 cells using a total protein extraction kit (Solarbio Life Sciences, Beijing, China) and the protein concentrations were determined using a Pierce BCA [bicinchoninic acid] Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions. Lysed 20 µg protein samples were separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes. Following blocking with 1% bovine serum albumin, the membranes were incubated overnight at 4℃ with primary rabbit antibodies against BCL2A1 (1:1000, Abcam, Cambridge, UK) and mouse anti-GAPDH antibodies (1:2000, ImmunoWay, Tennyson, TX, USA). The membranes were then incubated with horseradish peroxidase-conjugated secondary goat anti-rabbit and goat anti-mouse antibodies (1:5000, ImmunoWay, Tennyson, TX, USA) at room temperature for 2 h. Protein bands were visualized using an enhanced chemiluminescence (ECL) system and the gray values of the protein bands were quantified using the ImageJ software.
Statistical analysis
Statistical analysis was performed using GraphPad Prism 8.0.2 (GraphPad Software, San Diego, CA, USA). Differences between the two groups were analyzed using unpaired t-tests with Bonferroni correction and differences among multiple groups were analyzed using nonparametric one-way analysis of variance (ANOVA). All data were expressed as mean ± standard deviation (SD). All experiments were performed using at least three biological replicates. For all analyses, P < 0.05 was considered significant.
Results
Expression of miR-3976 was induced by C. parvum infection in HCT-8 cells
A previous study of the miRNA expression profile in HCT-8 cells suggested that miR-3976 expression was decreased at 12 h post-infection (hpi) [23]. RT-qPCR results confirmed that miR-3976 expression levels were significantly decreased at 8 and 12 hpi and increased at 24 and 48 hpi compared with the control group of uninfected cells (Fig. 1a).
MiR-3976 regulated cell apoptosis and parasite burden in HCT-8 cells after C. parvum infection
Functional analysis suggested that miR-3976 was involved in the regulation of apoptosis. To verify the role of miR-3976 in regulating cell apoptosis following C. parvum infection, we upregulated or downregulated its expression in HCT-8 cells by transfection with miR-3976 mimics or miR-3976 inhibitor, respectively (Fig. 1b), and then detected the cell apoptosis rate after infection. Flow cytometry revealed that the apoptosis rate was significantly increased in HCT-8 cells transfected with miR-3976 mimics compared with the NC group, and apoptosis was significantly decreased in the miR-3976 inhibitor group (Fig. 1c), suggesting that miR-3976 promoted HCT-8 cell apoptosis after C. parvum infection.
We also investigated the functional role of miR-3976 in regulating the parasite burden by RT-qPCR. The parasite burden was detected in the different groups of cells at 2 hpi, as a model for testing parasite attachment and cellular invasion, and at 48 hpi to assess parasite propagation/survival in cells [19, 20, 25]. There was no difference among the groups at 2 hpi. However, the parasite burden was increased at 48 hpi in cells transfected with miR-3976 mimics compared with the miR-3976 mimic NC group, while the opposite result was found in cells transfected with miR-3976 inhibitor (Fig. 1d). These results indicated that miR-3976 did not affect the initial parasite cellular invasion, but did inhibit the C. parvum burden in HCT-8 cells.
BCL2A1 was a target gene of miR-3976
TargetScan and miRBase were used to predict the potential target gene of miR-3976 [26, 27]. The prediction results indicated the presence of binding sites between miR-3976 and BCL2A1 (Fig. 2a). BCL2A1 expression levels were upregulated at different time points during C. parvum infection (Fig. 2b). Luciferase reporter activity was lower in cells co-transfected with BCL2A1-wild-type (WT) and miR-3976 mimics compared with cells co-transfected BCL2A1-WT and miR-3976 mimics NC, but not BCL2A1-MUT and pmirGLO vectors (Fig. 2c). Furthermore, overexpression of miR-3976 reduced the mRNA and protein levels of BCL2A1, while the opposite result was detected in cells with knockdown of miR-3976 using an inhibitor (Fig. 2d and e). These results demonstrated that miR-3976 targeted BCL2A1 and inhibited its expression, indicating that miR-3976 might regulate HCT-8 cell apoptosis via BCL2A1 in response to C. parvum infection.
MiR-3976 regulated cell apoptosis and parasite burden in HCT-8 cells by targeting BCL2A1 following C. parvum infection
We further explored the role of BCL2A1 in regulating HCT-8 cell apoptosis and parasite burden following C. parvum infection using the overexpression vector (pcDNA3.1-BCL2A1) or small interfering RNA (siRNA) (si-BCL2A1) to upregulate or knock down BCL2A1 expression, respectively, in HCT-8 cells (Fig. 3a). Similar to the results of inhibiting miR-3976, transfection with pcDNA3.1-BCL2A1 reduced the percentage of apoptotic cells, while knockdown of BCL2A1 with siRNA increased the apoptosis rate of HCT-8 cells after infection (Fig. 3b). No changes in parasite burden were detected among the different groups at 2 hpi (Fig. 3c); however the parasite burden was significantly increased at 48 hpi in cells transfected with pcDNA3.1-BCL2A1 compared with pcDNA3.1-NC, and the opposite result was detected in cells transfected with si-BCL2A1 (Fig. 3c).
To further determine whether miR-3976 targeted BCL2A1 to regulate HCT-8 cell apoptosis and parasite burden following C. parvum infection, we transfected HCT-8 cells with miR-3976 mimics and pcDNA3.1-BCL2A1 simultaneously. RT-qPCR showed that miR-3976 was markedly increased in cells co-transfected with miR-3976 mimics and pcDNA3.1-NC, compared with cells co-transfected with miR-3976 mimic NC and pcDNA3.1-NC; however, the increased expression of miR-3976 was eliminated when the cells were co-transfected with miR-3976 mimics and pcDNA3.1-BCL2A1, suggesting that miR-3976 directly targeted BCL2A1 (Fig. 3d). Similarly, flow cytometry showed that the increased apoptosis rate associated with miR-3976 expression was partially countered by overexpressed BCL2A1 (Fig. 3e), indicating that miR-3976 targeted BCL2A1 to regulate HCT-8 cell apoptosis following C. parvum infection. No changes in parasite burden were detected among the different groups at 2 hpi (Fig. 3f). Furthermore, co-transfection showed that the low parasite burden associated with miR-3976 expression in HCT-8 cells was significantly increased by pcDNA3.1-BCL2A1 at 48 hpi (Fig. 3f), suggesting that miR-3976 inhibited the C. parvum burden in HCT-8 cells by targeting BCL2A1.
Discussion
MiRNAs have been demonstrated to be involved in regulating the innate immune response to C. parvum infection [28]. The host’s innate immune response restricts parasite growth and initiates the adaptive immune response, which is necessary for parasite clearance and recovery [29]. Previous studies using an in vitro model of human biliary cryptosporidiosis showed that functional inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65-dependent miRNAs (miR-125b, miR-23b, and miR-30b) in cholangiocytes increased the C. parvum burden [25], but the underlying mechanisms remained unclear. Inhibition of miR-27b in host cells increased the C. parvum infection burden and regulated the epithelial defense against C. parvum infection in vitro [20]. In addition, the epithelial defense response against C. parvum was increased by upregulation of toll-like receptor 4 (TLR4) expression regulated by let-7i, and let-7i and TLR4 expression regulated the parasite burden and were involved in cholangiocyte immune responses against C. parvum infection in vitro [19]. In the current study, miR-3976 expression was induced by C. parvum infection and miR-3976 inhibited the C. parvum burden in HCT-8 cells by targeting BCL2A1, suggesting that this interaction may be involved in the host immune response against C. parvum infection by facilitating parasite clearance.
Cryptosporidium parvum has developed strategies of immune evasion to counteract the host defense response. Host cell apoptosis following invasion is a common antimicrobial strategy [30]. Previous studies suggested that C. parvum infection inhibited intestinal epithelial cell apoptosis during the early stage of infection and promoted host cell apoptosis at the late-infection stage [31]. B7-H1 (also known as programmed death-ligand 1) was induced by miR-513, thus contributing to the regulation of infected epithelial cell apoptosis and triggering apoptotic cell death by activating the Fas/FasL pathway or programmed cell death protein 1 signaling in bystander cells, including uninfected epithelial cells and infiltrating immune cells [17, 18]. The mechanism of apoptosis/anti-apoptosis may modulate parasite propagation and survival [16, 31]. In our study, miR-3976 targeted BCL2A1 to modulate HCT-8 cell apoptosis following C. parvum infection and miR-3976 also inhibited the C. parvum burden in HCT-8 cells by targeting BCL2A1. These results suggested that miR-3976 targeting of BCL2A1 may modulate the parasite burden in HCT-8 cells by regulating cell apoptosis. Further studies using a separation culture system of infected and uninfected cells should be carried out to clarify the relationship between cell apoptosis and parasite burden in infected cells.
BCL2A1 is a Bcl-2 anti-apoptotic protein involved in cell apoptosis, autophagy, inflammation, and other biological functions [32,33,34,35]. BCL2A1 transcription is highly regulated. BCL2A1 is an NF-κB target gene with an important role in the innate immune response. BCL2A1 expression was shown to depend on NF-κB activity, and can substitute for NF-κB to suppress tumor necrosis factor-α-induced apoptosis [36]. Previous studies suggested that activation of NF-κB inhibited infected epithelial cell apoptosis and facilitated parasite survival [37]. Activation of the TLR/NF-ĸB signaling pathway in host cells may trigger the transcriptional regulation of miRNA genes [38, 39]. However, it is unclear whether the NF-κB signaling pathway can regulate the expression of miR-3976 and whether NF-κB is involved in miR-3976 targeting of BCL2A1 to regulate cell apoptosis.
Conclusions
In conclusion, our results suggested that miR-3976 and BCL2A1 expression were induced by C. parvum infection, and that miR-3976 targeting of BCL2A1 may modulate the parasite burden in HCT-8 cells by regulating cell apoptosis in response to C. parvum infection. Future studies should determine the mechanism by which C. parvum infection regulates miR-3976 expression and the role of miR-3976 in host anti-C. parvum immunity in vivo.
Availability of data and materials
All remaining data discussed in this report are found in the main figures or the supplemental materials.
Abbreviations
- C. parvum :
-
Cryptosporidium parvum
- HCT-8 cells:
-
Human ileocecal adenocarcinoma cells
- 3′-UTR:
-
3′-Untranslated region
- KSRP:
-
KH-type splicing regulatory protein
- Fas:
-
Factor-associated suicide
- FasL:
-
Fas ligand
- RT-qPCR:
-
Real-time quantitative polymerase chain reaction
- BCL2A1 :
-
B-cell lymphoma 2-related protein A1
- NF-κB :
-
Nuclear factor kappa-light-chain-enhancer of activated B cells
References
Feng Y, Ryan UM, Xiao L. Genetic diversity and population structure of Cryptosporidium. Trends Parasitol. 2018;34:997–1011.
O’Hara SP, Chen XM. The cell biology of Cryptosporidium infection. Microbes Infect. 2011;13:721–30.
Laurent F, Lacroix-Lamandé S. Innate immune responses play a key role in controlling infection of the intestinal epithelium by Cryptosporidium. Int J Parasitol. 2017;47:711–21.
McDonald V, Korbel DS, Barakat FM, Choudhry N, Petry F. Innate immune responses against Cryptosporidium parvum infection. Parasite Immunol. 2013;35:55–64.
Ryan U, Hijjawi N, Xiao L. Foodborne cryptosporidiosis. Int J Parasitol. 2018;48:1–12.
Kotloff KL. The burden and etiology of diarrheal illness in developing countries. Pediatr Clin North Am. 2017;64:799–814.
Checkley W, Epstein LD, Gilman RH, Black RE, Cabrera L, Sterling CR. Effects of Cryptosporidium parvum infection in Peruvian children: growth faltering and subsequent catch-up growth. Am J Epidemiol. 1998;148:497–506.
Khalil IA, Troeger C, Rao PC, Blacker BF, Brown A, Brewer TG, et al. Morbidity, mortality, and long-term consequences associated with diarrhoea from Cryptosporidium infection in children younger than 5 years: a meta-analyses study. Lancet Glob Health. 2018;6:e758–68.
Guerrant RL. Cryptosporidiosis: an emerging, highly infectious threat. Emerg Infect Dis. 1997;3:51–7.
Innes EA, Chalmers RM, Wells B, Pawlowic MC. A one health approach to tackle Cryptosporidiosis. Trends Parasitol. 2020;36:290–303.
Checkley W, White AC Jr, Jaganath D, Arrowood MJ, Chalmers RM, Chen XM, et al. A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for Cryptosporidium. Lancet Infect Dis. 2015;15:85–94.
Jonas S, Izaurralde E. Towards a molecular understanding of microRNA-mediated gene silencing. Nat Rev Genet. 2015;16:421–33.
O’Connell RM, Rao DS, Chaudhuri AA, Baltimore D. Physiological and pathological roles for microRNAs in the immune system. Nat Rev Immunol. 2010;10:111–22.
Khan S, Ayub H, Khan T, Wahid F. MicroRNA biogenesis, gene silencing mechanisms and role in breast, ovarian and prostate cancer. Biochimie. 2019;167:12–24.
Abrahamsen MS, Templeton TJ, Enomoto S, Abrahante JE, Zhu G, Lancto CA, et al. Complete genome sequence of the apicomplexan, Cryptosporidium parvum. Science. 2004;304:441–5.
Ming Z, Zhou R, Chen XM. Regulation of host epithelial responses to Cryptosporidium infection by microRNAs. Parasite Immunol. 2017;39:e12408.
Gong AY, Zhou R, Hu G, Liu J, Sosnowska D, Drescher KM, et al. Cryptosporidium parvum induces B7–H1 expression in cholangiocytes by down-regulating microRNA-513. J Infect Dis. 2010;201:160–9.
Gong AY, Zhou R, Hu G, Li X, Splinter PL, O’Hara SP, et al. MicroRNA-513 regulates B7–H1 translation and is involved in IFN-gamma-induced B7–H1 expression in cholangiocytes. J Immunol. 2009;182:1325–33.
Chen XM, Splinter PL, O’Hara SP, LaRusso NF. A cellular micro-RNA, let-7i, regulates Toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection. J Biol Chem. 2007;282:28929–38.
Zhou R, Gong AY, Eischeid AN, Chen XM. miR-27b targets KSRP to coordinate TLR4-mediated epithelial defense against Cryptosporidium parvum infection. PLoS Pathog. 2012;8:e1002702.
Kapczuk P, Kosik-Bogacka D, Kupnicka P, Metryka E, Simińska D, Rogulska K, et al. The influence of selected gastrointestinal parasites on apoptosis in intestinal epithelial cells. Biomolecules. 2020;10:674.
Chen XM, Gores GJ, Paya CV, LaRusso NF. Cryptosporidium parvum induces apoptosis in biliary epithelia by a Fas/Fas ligand-dependent mechanism. Am J Physiol. 1999;277:G599-608.
Wang C, Liu L, Zhu H, Zhang L, Wang R, Zhang Z, et al. MicroRNA expression profile of HCT-8 cells in the early phase of Cryptosporidium parvum infection. BMC Genomics. 2019;20:37.
Zhang S, Jian F, Zhao G, Huang L, Zhang L, Ning C, et al. Chick embryo tracheal organ: a new and effective in vitro culture model for Cryptosporidium baileyi. Vet Parasitol. 2012;188:376–81.
Zhou R, Hu G, Liu J, Gong AY, Drescher KM, Chen XM. NF-kappaB p65-dependent transactivation of miRNA genes following Cryptosporidium parvum infection stimulates epithelial cell immune responses. PLoS Pathog. 2009;5:e1000681.
Ritchie W. microRNA target prediction. Methods Mol Biol. 2017;1513:193–200.
Griffiths-Jones S, Grocock RJ, van Dongen S, Bateman A, Enright AJ. miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res. 2006;34:D140–4.
Zhou R, Feng Y, Chen XM. Non-coding RNAs in epithelial immunity to Cryptosporidium infection. Parasitology. 2014;141:1233–43.
Crawford CK, Kol A. The mucosal innate immune response to Cryptosporidium parvum, a global one health issue. Front Cell Infect Microbiol. 2021;11:689401.
Barber GN. Host defense, viruses and apoptosis. Cell Death Differ. 2001;8:113–26.
Liu J, Deng M, Lancto CA, Abrahamsen MS, Rutherford MS, Enomoto S. Biphasic modulation of apoptotic pathways in Cryptosporidium parvum-infected human intestinal epithelial cells. Infect Immun. 2009;77:837–49.
Kathania M, Raje CI, Raje M, Dutta RK, Majumdar S. Bfl-1/A1 acts as a negative regulator of autophagy in mycobacteria infected macrophages. Int J Biochem Cell Biol. 2011;43:573–85.
Noble KE, Wickremasinghe RG, DeCornet C, Panayiotidis P, Yong KL. Monocytes stimulate expression of the Bcl-2 family member, A1, in endothelial cells and confer protection against apoptosis. J Immunol. 1999;162:1376–83.
Vier J, Groth M, Sochalska M, Kirschnek S. The anti-apoptotic Bcl-2 family protein A1/Bfl-1 regulates neutrophil survival and homeostasis and is controlled via PI3K and JAK/STAT signaling. Cell Death Dis. 2016;7:e2103.
Li X, Dou J, You Q, Jiang Z. Inhibitors of BCL2A1/Bfl-1 protein: potential stock in cancer therapy. Eur J Med Chem. 2021;220:113539.
Zong WX, Edelstein LC, Chen C, Bash J, Gélinas C. The prosurvival Bcl-2 homolog Bfl-1/A1 is a direct transcriptional target of NF-kappaB that blocks TNFalpha-induced apoptosis. Genes Dev. 1999;13:382–7.
Chen XM, Levine SA, Splinter PL, Tietz PS, Ganong AL, Jobin C, et al. Cryptosporidium parvum activates nuclear factor kappaB in biliary epithelia preventing epithelial cell apoptosis. Gastroenterology. 2001;120:1774–83.
Zhang G, Zhang Y, Niu Z, Wang C, Xie F, Li J, et al. Cryptosporidium parvum upregulates miR-942-5p expression in HCT-8 cells via TLR2/TLR4-NF-κB signaling. Parasit Vectors. 2020;13:435.
Feng R, Niu Z, Zhang X, Hou W, Zhang Y, Jian F, et al. Cryptosporidium parvum downregulates miR-181d in HCT-8 cells via the p50-dependent TLRs/NF-κB pathway. Vet Parasitol. 2022;305:109710.
Acknowledgements
We thank International Science Editing (http://www.internationalscienceediting.com) for editing this manuscript.
Funding
This research was supported by grants from the National Key Research and Development Program of China (2022YFD1800200), the National Natural Science Foundation of China (U2004155), and the Science Fund for Distinguished Young Scholars of Henan Province (222300420010).
Author information
Authors and Affiliations
Contributions
RW and XL conceived and designed the experiments; JL and LS performed the experiments; FX, TS, LZ, and SW analyzed the data and prepared the figures; JL and RW wrote the paper. All authors reviewed the manuscript.
Corresponding authors
Ethics declarations
Ethics approval and consent to participate
The present study was approved by the Independent Ethics Committee of Henan Agricultural University (approval no. IEC-HENAU-20180323-06).
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Additional file 1: Table S1.
Primers used in RT-qPCR and sequences used to generate constructs.
Additional file 2: Table S2.
RNA oligonucleotides for miRNAs and siRNAs.
Additional file 3: Table S3.
The inserted sequence of BCL2A1-WT and BCL2A1 MUT.
Additional file 4: Figure S1.
The overexpression plasmid map of BCL2A1.
Additional file 5.
The sequences of pmirGLO-BCL2A1-WT and pmirGLO-BCL2A1-MUT.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Li, J., Sun, L., Xie, F. et al. MiR-3976 regulates HCT-8 cell apoptosis and parasite burden by targeting BCL2A1 in response to Cryptosporidium parvum infection. Parasites Vectors 16, 221 (2023). https://doi.org/10.1186/s13071-023-05826-w
Received:
Accepted:
Published:
DOI: https://doi.org/10.1186/s13071-023-05826-w