Abstract
Controlled mRNA storage and stability is essential for oocyte meiosis and early embryonic development. However, how to regulate mRNA storage and stability in mammalian oogenesis remains elusive. Here we showed that LSM14B, a component of membraneless compartments including P-body-like granules and mitochondria-associated ribonucleoprotein domain (MARDO) in germ cell, is indispensable for female fertility. To reveal loss of LSM14B disrupted primordial follicle assembly and caused mRNA reduction in non-growing oocytes, which was concomitant with the impaired assembly of P-body-like granules. 10× Genomics single-cell RNA-sequencing and immunostaining were performed. Meanwhile, we conducted RNA-seq analysis of GV-stage oocytes and found that Lsm14b deficiency not only impaired the maternal mRNA accumulation but also disrupted the translation in fully grown oocytes, which was closely associated with dissolution of MARDO components. Moreover, Lsm14b-deficient oocytes reassembled a pronucleus containing decondensed chromatin after extrusion of the first polar body, through compromising the activation of maturation promoting factor, while the defects were restored via WEE1/2 inhibitor. Together, our findings reveal that Lsm14b plays a pivotal role in mammalian oogenesis by specifically controlling of oocyte mRNA storage and stability.
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Introduction
In mammals, the number of available primordial follicle is a major factor which determines the reproductive life span. The number of available oocytes is determined immediately after birth in mice. Therefore, the establishment of primordial follicle pool is important for reproductive sustainability [1]. The primordial follicle populations are considered a finite resource, with any disturbances in the assembly process potentially affecting a woman’s overall oocyte availability and increasing the risk of conditions such as premature ovarian insufficiency [2, 3]. It is noteworthy that the oocytes stored in the primordial follicles, referred to as non-growing oocytes (NGO), while only a limited number of oocytes are activated and developed, referred to as growing oocytes (GO) [4]. Along with the oocyte growth, it accumulates maternal mRNA in the cytoplasm before reaching fully grown oocytes (FGO) [5].
Maternal mRNA required for oocyte maturation and early embryonic development must accumulate continuously and steadily during oocyte growth [4, 6, 7]. However, the mechanism of maternal mRNA storage remains elusive during oogenesis. In general, maternal mRNA associates with numerous ribonucleoprotein complexes and accumulates in membraneless compartments by phase separation, such as P-body-like granules and mitochondria-associated ribonucleoprotein domain (MARDO) [8,9,10,11,12]. Previous studies have established the presence of scattered P-body-like granules in the oocyte cytoplasm, which serves as a repository for genes associated with the primordial follicle during the establishment of the primordial follicle pool [13]. Constituting processing bodies (P-bodies) are a major class of proteins (LSM1-7, DCP1/2, EDC3, EDC4, UPF1, YTHDF2, DDX6, LSM14A, YBX2) associated with mRNA demethylation [14,15,16,17,18] and another class including RNA-binding proteins (4E-T/EIF4ENIF1, CPEB1, DYNC1H1) [19,20,21] that promote translation inhibition [22, 23]. A recent study has demonstrated that the absence of DDX6, the central component of P-body-like granules, impairs the primordial follicle assembly, indicating that P-body-like granules is essential in maintaining primordial follicle assembly [13]. Notably, LSM14B interacts specifically with DDX6, which is detected in polysomes and stagnant translation-initiating complexes, playing a role in inhibiting translation [24]. Subsequently, together with ZAR1, YBX2, and 4E-T as core components of MARDO, they were reported to store mRNA in mammalian FGO, ensuring stable maturation of oocytes. Zar1 is one of the maternal-effect genes that regulate transcription activation and precisely control early oogenesis and maternal mRNA homeostasis [6, 25]. Similarly, Ybx2 acts as oocyte-specific gene that regulates primordial follicle assembly and follicle growth [26, 27]. Given that the accumulation of maternal mRNA begins at an early oogenesis [4], it is reasonable to infer that these membraneless compartment core protein involved in maternal mRNA storage already play a role in the early stage of oogenesis [28,29,30].
LSM14B, represented by RAP55, belongs to the RNA-associated protein and is involved in the assembly of P-body and MARDO [24, 31]. Previous results have found that LSM14B affects meiotic processes and oocyte maturation by regulating the activities of spindle assembly checkpoints and maturation promoter factor (MPF) [32]. However, there is currently a lack of direct evidence to support the contribution of LSM14B to the storage of maternal mRNA during oogenesis.
In this study, we found that LSM14B is one of the components of P-body-like granules and forms aggregate-like foci that co-localized with DDX6 during the primordial follicle formation. Depletion of LSM14B leads to P-body-like granules structure destruction and mRNA decay and affects primordial follicle assembly and maternal mRNA accumulation. Moreover, we found that reduced MPF activity in Lsm14b knockout (KO) oocytes led to the formation of pronucleus (PN) after extrusion of the first polar body (PB1), which was eliminated by activation of CDK1. Our results confirm that LSM14B is specifically expressed in oocytes and is involved in the stabilization and storage of maternal mRNA.
Materials and methods
Animals
All mouse strains had a C57BL/6 background. Lsm14b KO mouse strains were made using the CRISPR/Cas9 system, as shown in Fig S2B. Mice were maintained under specific pathogen-free conditions in a controlled environment at 22 ± 2℃ with a 12/12 h light/dark cycle, 45–60% humidity, and free access to food and water. Mice were genotyped by PCR using primers as shown in Table S1, which produces a 453 and 600 bp PCR product for the wild type (WT) and Lsm14b KO alleles, respectively. All animal experiments were approved and performed in line with the Animal Care and Use Committee at Inner Mongolia University (IMU-MOUSE-2023-013).
Co-immunoprecipitation
The ovarian samples were lysed by adding 50 mg of tissue in 600 μl IP buffer containing 20 mM Tris–HCl, 10 mM EDTA, 1 mM EGTA, 150 mM NaCl, 0.05% Triton X-100, 0.05% NP-40, and 1 mM PMSF. Prior to use, protease (Sigma-Aldrich, P8340, 1:100) and phosphatase inhibitors (Sigma-Aldrich, P5726, 1:500) were added. Protein A&G agarose beads were washed and then incubated with anti-IgG and anti-LSM14B at 4 °C for 4 h, respectively. Ten microliters of both IgG and LSM14B rabbit antibodies were used. Antibody combined beads were collected and incubated with lysates at 4 °C overnight. Beads were washed three times and boiled for 5 min. The SDS-PAGE was run for immune blotting, followed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI/MS) analysis. The gel samples were sent to the proteomics facility at Shanghai Applied Protein Technology to undergo MALDI/MS. Original blots can be found in the Source data file.
Immunohistochemistry
Ovarian samples were collected from each group, fixed with 4% paraformaldehyde, embedded in paraffin, and continuously sectioned at a thickness of 5 μm. Tissue sections were subjected to gradient alcohol dewaxing and incubated with blocking solution (3% BSA, 10% normal donkey serum in TBST) for 30 min. After overnight incubation with primary antibodies (DDX4, LSM14B, and DDX6 in Table S2) at 4 °C followed by incubation with fluorescein-conjugated secondary antibody (Table S2), nuclei were stained with DAPI after washing with TBST.
Hematoxylin–eosin (H&E) staining
Ovaries were fixed for overnight at 4℃ temperature in Bouin’s fixative (Sigma, HT10132), followed by ethanol dehydration, xylene clarification, and paraffin embedding. Ovarian blocks were serially sectioned at 5 μm thickness. After drying at 37 °C, the samples were stained with Lillie-Mayer hematoxylin (Solarbio, G1080, China) and Eosin (Zsbio, ZLI-9613, China). Follicles at different stages of development were counted in every three sections of the whole ovary, and the total number of each stage follicles was calculated as described previously. To avoid double counting the same follicle, only follicles containing oocytes with visible nuclei were counted and classify the follicles according to their characteristics.
Fertility identification
Fertility tests were carried out by mating 8-week-old WT and Lsm14b KO female mice with WT male mice for 6 months. The number of pups for each cage was recorded at birth, and the average number of pups per cage was calculated.
Tissue immunofluorescence
After fixing the freshly isolated ovaries in 4% paraformaldehyde (PFA) prepared in PBS overnight then infiltrated in paraffin (Leica, 39,601,095, Germany), sample sections of 5 μm in thickness were blocked at room temperature for 30 min after gradient rehydration and antigen retrieval and incubated with primary antibodies (LSM14B, DDX4 in Table S2) overnight at 4˚C. After 3 washes in TBST, the sections were incubated with secondary antibodies of goat anti-rabbit IgG (Table S2) for 30 min at room temperature. Expressed immunoreactive signals were visualized by DAB kit (ZSGB-BIO, ZLI-9017, China) and nuclei were counterstained with hematoxylin. After dehydration, the slide was immediately sealed with neutral balsam.
Single-cell libraries and sequencing
With qualified single-cell sample, cells in 0.04% BSA in PBS were loaded onto 10 × Chromium chip, and single-cell gel beads in emulsion were generated by using Single Cell 30 Library and Gel Bead Kit V2 (10 × Genomics Inc., 120,237, Pleasanton, California, USA). Following the manufacturer’s instructions, single-cell RNA-seq libraries were constructed, and pair-end 150 bp sequencing was performed to produce high-quantity data on an Illumina HiSeq X Ten (Illumina, San Diego, California, USA).
The raw data were analyzed and aggregated using Cell Ranger (version 4.0) to obtain barcode tables and gene expression matrices. Afterward, we imported the matrix into Seurat (version 4.0.2) for quality control. Data integration was done through the “FindIntegrationAnchors” and “IntegrateData” functions. To divide the various types of cell clusters, the “RunUMAP” function was used for cell clustering and visualization. In addition, differential analysis of germ cells and granulosa cells in the WT and Lsm14b KO ovaries was done by “FindMakers.” We considered genes with P < 0.05 and |Log2(FC)|> 0.25 to be significantly different. Next, we performed pseudotime cell trajectory construction on the gene count matrix based on the R package Monocle (version 2.18.0). This process used the “reduceDimension” and “orderCells” functions.
Superovulation and fertilization
In superovulation, female mice (6 to 8 weeks old) were intraperitoneally injected with 5 IU of PMSG for 48 h. Subsequently, they were then injected with 5 IU of hCG, and oocyte cumulus complexes were removed from the oviducts 15 h later, and the numbers of oocytes were counted after digestion with 0.3% hyaluronidase for 10 min. For in vitro fertilization (IVF), TYH medium (EasyCheck, M2050, China), HTF medium (EasyCheck, M1130, China), and in vitro maturation (IVM) medium (EasyCheck, M2115, China) were put into 37 °C in 5% CO2 atmosphere overnight. On the second day, the epididymis of adult male mice was removed and transferred to the capacitation fluid for 1 h. Subsequently, the oocytes were transferred into the fertilization drop, and the spermatozoa in 10 μl TYH medium were taken out and added to the fertilization drop. After the fertilization for 6 h, the unfertilized sperm were washed with IVM medium, and the fertilized oocytes were transferred into the HTF medium. Formation of 2-cell stage embryos was scored 24 h after IVF and development of 2-cell embryos to the blastocyst stage was scored 3–5 days after culture.
Oocyte culture
The 6-week-old female mice were injected with 5 IU of PMSG and humanely euthanized 48 h later. The GV-stage oocytes were harvested in M2 medium (Sigma, M7167, Japan) and cultured in 100 μl of M2 medium covered with mineral oil (Sigma, M8410, Japan) at 37 °C in a 5% CO2 atmosphere. During culture, the stage of GV, Pro-metaphase I (pre-MI), Metaphase I (MI), Anaphase I (AI), Telophase I (TI), and Metaphase II (MII) oocytes were collected at the time points of 0, 4, 6, 8, 10, and 14 h.
Confocal microscopy of mouse oocytes
Oocytes were fixed with 4% paraformaldehyde. Then they were permeabilized with 0.3% Triton X-100 in PBS for 30 min. After being blocked with 1% bovine serum albumin in PBS, the oocytes were incubated with primary antibodies for 1 h and sequentially incubated with anti-α-tubulin-FITC antibody (Sigma, F2168, Japan) overnight. Finally, the oocytes were washed three times with the TBST and then stained with Hoechst 33,342 (Solarbio, C0031, China) for 15 min. Imaging was carried out using a confocal laser-scanning microscope (A1R, Nikon, Japan).
Western blot analysis
Proteins were collected from P3 ovaries and GV and MII oocytes by adding RIPA (addition of protease inhibitors) and vortexing on ice, followed by boiling with an equal volume of 2 × SDS loading buffer for 10 min. Samples were separated by SDS-PAGE, transferred to PVDF membranes, blocked with 5% non-fat dry milk in TBST for 1 h at room temperature, and incubated with primary antibody (LSM14B, DDX6, p-CDK1(Y15), CCNB1, DDX4, β-ACTIN in Table S2) at 4 °C overnight. Then, membranes were washed with TBST and incubated with secondary antibodies for 1 h at room temperature. Chemiluminescence was performed using a BeyoECL Plus Kit (Beyotime, A0018), as directed by the manufacturer.
RNA extraction and real-time quantitative PCR (RT-qPCR)
Total RNA was extracted from oocytes, testis, muscle, brain, kidney, lung, spleen, and liver of WT and Lsm14b KO mice according to the instructions of the EasyPure® RNA Kit (TransGen Biotech, ER101, Beijing, China), and the subsequent cDNA samples were subjected to reverse transcription Kit (TransGen Biotech, AT311) and RT-qPCR was performed in 10 µl reactions using a Light Cycler 480II System (Roche Diagnostics). The 2(−ΔΔCt) method was used to analyze RT-qPCR data and data were normalized to Gapdh. Primer sequences for the targeted mouse genes are listed in Table S1.
RNA-seq analysis
Transcriptomic analysis of WT and Lsm14b KO GV-stage oocytes was performed according to the protocol provided by the company. Briefly, per 20 fully grown oocytes were removed into individual tubes that contained 10 µl of lysis buffer. According to the manufacturer’s instructions, reverse transcription reaction was carried out directly on the whole cell lysate using Illumina Sequencing-HV kits (Takara Bio Inc, Japan). Sequencing libraries were constructed from amplified cDNA. Barcoded libraries were pooled and sequenced on Illumina HiSeq 4000 platform with 150 bp pair-end reads. All the raw reads were trimmed to remove low-quality bases and adaptor sequences. The clean reads were then mapped to the mouse genome with STAR (version 2.7.10). The expression levels of each gene were quantified using featureCounts software (version 2.0.3). Differentially expressed genes were calculated using default parameter of DESeq2 (version 1.30.1).
Proteomic analysis
For each group of 500 GV-stage oocytes, SDT lysis method was used to extract protein, and then BCA method was used for protein quantification. An appropriate amount of protein was taken from each sample for trypsin digestion by filter-aided proteome preparation (FASP) method, using C18 Cartridge. The peptides were desalted, lyophilized, and reconstituted with 40 μl of 0.1% formic acid solution, and the peptides were quantified (OD280). Each sample was separated using the NanoElute HPLC liquid system at nanoliter flow rates. The samples were chromatographically separated and analyzed by mass spectrometry using a timsTOF Pro mass spectrometer. The detection method was positive ion, the ion source voltage was set to 1.5 kV, and both MS and MSMS were detected and analyzed by TOF. The MS scan range was set to 100–1700 m/z. The data acquisition mode adopts the parallel accumulation serial fragmentation (PASEF) mode. After the first-order mass spectrum was acquired, 10 ions were collected in the PASEF mode, with a cycle window time of 1.17 s. The raw data of mass spectrometry analysis were RAW files, and MaxQuant software (version 1.6.14) was used for library identification and quantitative analysis. Differentially expressed proteins were calculated using default parameter of DESeq2 (version 1.30.1).
Gene Ontology and KEGG enrichment analysis
The R package clusterProfiler (version 3.18.1) was used to perform Gene Ontology and KEGG enrichment analysis on the differential genes [33], and the threshold was set to “P < 0.05” to match the highly correlated biological processes and signaling pathways of the differential genes.
Statistical analysis
All statistical analyses were performed using GraphPad Prism software (version 8.0). Data are presented as means ± SEM. Student’s t-tests were used to compare differences between two groups, with P < 0.05 defined as significantly different. One-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison’s test was used to assess the statistical significance of differences among three groups. Differential gene/protein were defined as those with variable importance in the projection value greater than 1.0 and P-adjusted < 0.05.
Results
LSM14B is a component of P-body-like granules and MARDO within germ cells
MARDO have been shown to regulate maternal mRNA storage, translation, and decay in fully grown oocytes (FGOs) [34]. Notably, we found that gene expression levels of Lsm14b, Ybx2, Ddx6, Eif4enif1, and Zar1, which are the compositions of MARDO, were significantly increased in oocytes from E14.5 to postnatal day 6 (P6) (Fig. 1a) [4], indicating that MARDO members may be functional in early oogenesis. Immunostaining analysis demonstrated that LSM14B co-localized with germ cell-specific marker DDX4 in all stages of follicles, which aligns with the findings of transcriptomic analysis of ovarian cells (Fig. 1b), suggesting a potential role for LSM14B in oocyte. Furthermore, to identify the potential interacting partners of LSM14B, we performed co-immunoprecipitation (Co-IP) of LSM14B protein followed by mass spectrometry (MS) analysis and revealed that the interacting proteins, including DDX6, YBX2, CPEB1, ZAR1, EDC4, LSM5, and 4E-T, are not only involved in the constitution of MARDO but also are the part of P-body (Fig. 1d, Supplementary Fig. 1a, b). Previous studies have established that RNA-binding proteins YBX2 [35] and ZAR1 [34] are required for maternal mRNA storage and metabolism. The DDX6 and 4E-T interaction mediates translational repression and de novo P-body assembly [19]. Previous study also indicated that LSM14B specifically interacts with DDX6, which plays a pivotal role in the regulation of mRNA metabolism during the early developmental stage of Xenopus oocyte [24]. Moreover, loss of DDX6 disturbs the P-body-like granules formation and severely impairs the assembly of primordial follicle in mice [13]. Similarly, the Co-IP experiment indicated that LSM14B indeed interacts with DDX6 in mouse oocytes (Fig. 1e). Importantly, immunofluorescence staining showed that LSM14B was co-localized with DDX6 and presented aggregate-like foci in germ cells (Fig. 1f). These observations suggest that LSM14B is involved in the composition of the membraneless compartment including P-body-like granules and MARDO, although the role of LSM14B remained unknown in mammalian oocytes.
Knockout of Lsm14b compromises female fertility and primordial follicle pool
The expression level of Lsm14b in different tissues was detected by RT-qPCR and found to be highly expressed in oocyte (Supplementary Fig. 2a). To examine the functions of LSM14B in oocyte, we generated Lsm14b knockout (Lsm14b KO) mice (Supplementary Fig. 2b–d). Predictably, LSM14B protein expression was not detected in different classes of follicles in mutant mice (Fig. 2a). We found no significant changes in body weight, ovarian size, or ovulation number in 8-week-old female Lsm14b KO mice (Supplementary Fig. 2e–g). Fertility testing revealed that Lsm14b KO female mice were completely infertile after crossing with WT male mice (Fig. 2b). Eggs ovulated by 8-week-old Lsm14b KO mice could be fertilized. Nevertheless, over 90% of the Lsm14b♀−/♂+ embryos failed to develop to the 2-cell stage, compared to the WT group (Fig. 2c, d). Notably, we also found that a number of follicles in the ovaries of Lsm14b KO mice at 6 months were nearly exhausted compared to WT mice (Fig. 2e; Supplementary Fig. 2 h), suggesting that Lsm14b KO caused premature ovarian failure. Given that the LSM14B presented aggregate-like foci in the primordial follicles, we detected the numbers of the primordial follicle. The results show that the numbers of primordial follicle in the ovaries of Lsm14b KO mice at 2, 3, and 8 weeks were significantly reduced compared to WT mice (Fig. 2f–i). Based on the results of primordial follicle loss in mutant ovaries, we concluded that Lsm14b plays an integral role during the primordial follicle assembly.
LSM14B is essential for primordial follicle assembly
Primordial follicle assembly is a finely regulated complex process, and it determines the length of the reproductive life in female. We observed that the percentage of germ cells in primordial follicle was dramatically decreased, and a total number of germ cells were not lost in the P3 ovaries of Lsm14b KO mice (Fig. 3a–c). These results indicated that primordial follicle assembly is severely compromised in Lsm14b KO ovaries.
To characterize the temporal and mechanistic features of impaired primordial follicle assembly in Lsm14b KO ovaries, a 10 × Genomics single-cell atlas of ovary was performed, and then we identified and extracted germ cell clusters based on data quality control, UMAP cluster analysis, and known cell-specific markers [1] (Supplementary Fig. 3a–d). Notably, germ cell clusters were labeled as nine cell clusters by UMAP analysis, whereas germ cells in WT and Lsm14b KO mice were distributed in distinct cell clusters (Fig. 3d, Supplementary Fig. 3e). This suggested that the absence of LSM14B induced divergent transcriptional profiling of germ cells.
To explain this abnormal cellular distribution, we performed a pseudotime trajectories analysis. There are three states of cells at P3 in the germ cells of WT mice. Based on the dynamic expression of Id1 and Ooep on pseudotime trajectories, state 1 was assigned as “Follicle” and state 3 as “Nest” (Fig. 3e (a), Supplementary Fig. 3f (a)). By contrast, germ cells of Lsm14b KO mice adopted different branches and had a multipath differentiation process (Fig. 3e (b); Supplementary Fig. 3f (b)). It is worth noting that when the germ cells of the two groups were placed on the same pseudotime developmental process, it could be found that most of the Lsm14b KO germ cells were arrested at the “Nest” stage and could not become “Follicle” (Fig. 3f-g; Supplementary Fig. 3 g). Interestingly, granulosa cells, an important player in primordial follicle assembly, were also affected by deletion of Lsm14b. Two groups of granulosa cells were distributed in different cell clusters (Supplementary Fig. 4a, b). And granulosa cells in WT P3 ovaries arranged in a linear trajectory, while the development process of Lsm14b KO granulosa cells was disrupted (Supplementary Fig. 4c). These results further indicated that transcriptional profiling of primordial follicle assembly was disrupted in Lsm14b KO mice.
To evaluate the influence of Lsm14b deficiency on gene expression, a time-course transcriptomic differences were assessed by scRNA-seq analyses in WT oocytes from E16.5, P0, and P3. We found that consistent with the timing of primordial follicle formation, 4,964 genes (more than threefold) were greatly increased in germ cell from E16.5 to P3. Among these 4,964 genes, there were tenfold more genes with significantly down-regulated expression levels than with significantly up-regulated expression levels in Lsm14b KO P3 oocytes compared with WT P3 oocytes (Fig. 3h). We observed that the expression levels of genes associated with the assembly and quality of primordial follicle in the Lsm14b KO ovaries were significantly lower than in the WT ovaries (Fig. 3i). Meanwhile, WB results showed that the protein levels of LHX8 and NOBOX in 3d Lsm14b KO ovaries were also significantly decreased (Fig. 3j). Our results confirm that LSM14B is indispensable for the primordial follicle assembly process.
Lsm14b deletion impaired the assembly of P-body-like granules
It was previously reported that P-body-like granules are critical for primordial follicle assembly in mice [13]. Given that LSM14B was co-localized with DDX6 and formed aggregate-like foci in newborn ovaries, we investigated whether the absence of LSM14B affects the assembly of P-body-like granules. Western blot analyses showed that DDX6 was present at normal levels in Lsm14b KO group compared with the WT group (Fig. 4a). Notably, immunostaining of P-body-like granules components revealed that the assembly of P-body-like granules was impaired in Lsm14b KO ovaries (Fig. 4b). Next, we measured the area of DDX6 foci per unit area (μm2) of nest and follicle. Statistical analysis revealed a significant reduction in DDX6 lesion area in Lsm14b KO ovaries relative to WT ovaries (Fig. 4c). The results suggest that Lsm14b may be involved in the assembly of P-body-like granules in oocytes.
Considering the P-body components play crucial roles in mRNA metabolism [18, 30], we investigated whether disruption of P-body-like granules affects dynamics of mRNA in oocytes. The scRNA-seq data revealed that more transcripts were found to be down-regulated by at least 50% (2519 down and 1185 up, respectively) in Lsm14b KO NGO, as compared with the WT NGO (Fig. 4d). Gene enrichment analysis revealed that the down-regulated mRNAs were mainly associated with processes related to “mRNA metabolism and translation,” “oocyte meiosis and cell cycle,” and “mitochondrion organization and oxidative phosphorylation” (Fig. 4e). It is worth noting that P-bodies were highlighted to be involved in processes similar to “mRNA metabolism and translation” and “oocyte meiosis and cell cycle” [22, 36, 37]. Interestingly, similar dynamics of mRNA in granulosa cells were also exhibited in Lsm14b KO group (Supplementary Fig. 4d–f). Moreover, we found the decreased mRNA expression levels of several of P-body-like granules components in Lsm14b KO oocytes (Fig. 4f). These results suggest that many mRNAs involved in the regulation of oocyte development are stored in P-body-like granules and that the absence of LSM14B not only disrupts P-body-like granules function but also causes mRNA reduction.
Loss of LSM14B disrupts maternal mRNA accumulation and translation in FGO
Previously results indicated that deletion of ZAR1 impaired maternal mRNA accumulation in oocyte, and loss of ZAR1 disrupts MARDO formation and mRNA storage, translation, and degradation during oocyte maturation [6]. Since LSM14B is predicted RNA-binding proteins [38], and LSM14B deletion causes mRNA reduction, we investigated the effects of LSM14B deletion on maternal mRNA in FGO.
We performed RNA-seq analysis of GV-stage oocytes of Lsm14b KO and WT mice (Fig. 5a). A total of 1,677 transcripts were identified to be significantly changed, of which 946 transcripts were down-regulated and 731 were up-regulated, respectively, in the Lsm14b KO oocytes as compared with WT oocytes (Fig. 5b). By performing Gene Ontology analysis on the list of differential transcripts, we found that the expression levels of transcripts involved in “mitochondrial” and “translational functions” were significantly down-regulated. In addition, the expression levels of transcripts associated with protein phosphorylation were abnormal (Fig. 5c, d). Phosphorylation is a well-known mechanism for regulating the assembly and disassembly of membraneless compartments [39]. The Gene Set Enrichment Analysis (GSEA) results showed that in addition to most down-regulation of ribosome and translation-related transcripts, abnormal expression of transcripts involved in oocyte arrest, embryo development, and actin filaments could also be observed (Supplementary Fig. 5a, b). These results suggest that transcriptome was disrupted in Lsm14b KO oocytes. To further confirm whether the loss of Lsm14b affects the accumulation of maternal mRNA, we compared the differential transcripts of the two groups of NGO and FGO with the maternal mRNA, respectively. More maternal mRNAs could be observed to be down-regulated in Lsm14b KO NGO and FGO (Fig. 5e–h). The number of genes in the down-regulated maternal mRNA was nearly fivefold the number of up-regulated maternal mRNA genes (Fig. 5e, f) and is nearly twofold the number of up-regulated maternal mRNA genes in FGO (Fig. 5g, h), which also suggest that the structure of storing maternal mRNA in NGO and FGO is destroyed. A series of lines of evidence have shown that loss of Lsm14b affects the maternal mRNA accumulation and storage.
Considering that translation-related transcripts were significantly altered in FGO, we detected the protein level of GV oocytes. In protein mass spectrometry analysis of GV oocytes, we found significant clustering in WT and Lsm14b KO groups (Supplementary Fig. 5c). This further suggested that loss of Lsm14b affected translation in GV oocytes. Next, we performed GSEA on the differential proteins, and the results showed that mRNA metabolism, translation, and cell cycle-related proteins were significantly down-regulated (Supplementary Fig. 5d–e). These lines of evidence demonstrate that loss of LSM14B disrupts translational progression in GV oocytes. Furthermore, we observed that loss of Lsm14b resulted in down-regulation of protein levels of MARDO-associated proteins (EIF4ENIF1, YBX2, ZAR1, DDX6, and LSM14B) (Fig. 5i). It was also observed that the depletion of LSM14B leads to a decrease in DDX6 protein levels by Western blot detection of GV and MII oocytes (Fig. 5j, k). Collectively, these results suggest that the absence of LSM14B affects MARDO function and disrupts maternal mRNA accumulation and translation in GV oocytes.
Defective meiotic progression in Lsm14b KO oocytes
Lsm14b KO female mice were found to be completely infertile (Fig. 2b), but the ovulation of Lsm14b KO female mice was normal (Supplementary Fig. 2 g). Given that oocyte quality is a key limiting factor in female fertility, the female infertility in Lsm14b KO female mice could be attributed to decline in oocyte quality [40]. Notably, we found that although Lsm14b KO oocytes completed meiosis I with normal cytokinesis and extruded morphologically normal PB1 in vivo almost of the Lsm14b KO oocytes contained a visible PN (Fig. 6a, b). IF staining revealed that residual central spindle was still visible between the decondensed chromatin and PB1 in nearly 80% of Lsm14b KO oocytes (Fig. 6c, d). It is well known that the spindle of the oocyte moves to the cortex and homologous chromosomes begin to separate during AI, and cytoplasmic division occurs when TI is reached; then meiosis enters MII with no intermediate phase [41, 42]. However, we found that oocytes fail to assemble the MII spindle after extrusion of the PB1 and enter into interphase and form a PN by using IF staining of oocytes after 8–15 h of hCG treatment (Fig. 6e). To confirm this result, GV oocytes were collected from ovaries and then cultured further in M16 medium for IVM. Consistent with the results in vivo, Lsm14b KO oocytes specifically form PN after cultured 16 h in vitro (Supplementary Fig. 6a, b).
Considering the MPFs (regulatory subunit CCNB1 and catalytic subunit CDK1) play indispensable roles in control of oocyte meiotic progression [43], we investigated the possibility whether knockout of Lsm14b affects the activation of MPF. We measured the levels of CCNB1 and p-CDK1 (Y15) in ovulated oocytes by Western blot analysis and found that the expression of CCNB1 was reduced and the levels of p-CDK1 (Y15) were significantly increased compared with WTs (Fig. 6f). Our results suggest that significantly increased levels of p-CDK1 (Y15) are probably the causes of the meiotic defects in Lsm14b KO oocytes. Previous studies have shown that PD166285, a specific inhibitor of WEE1/2 kinases, could be able to activate MPF by reduced levels of CDK1 (Y15) phosphorylation in mouse oocytes [44, 45]. Western blot analysis showed that PD166285 treatment dramatically reduced the levels of CDK1 (Y15) phosphorylation in Lsm14b KO GV oocytes at the end of 20 h IVM (Fig. 6g). Coincidently, treatment with the 10 µM PD166285 could prevent PN formation by the end of 20 h culture (Fig. 6h, i). These results confirmed that LSM14B is required for oocyte MII arrest.
Discussion
The maternal mRNA is transcribed and transcripts accumulate during oocyte growth in mammals [46], and these mRNAs must be stored in membraneless compartments and then activated at the proper time in development. The role of membraneless compartments have been reported in non-mammalian oocytes, but less so in mammalian oocytes [8,9,10,11, 47, 48]. Maternal mRNA storage compartments have been characterized in non-growing and growing mouse oocytes and named this compartments the P-body-like granules [13]. In addition, it was previously reported that fully grown human and mouse oocytes accumulate mRNAs in a MARDO [34]. In this study, we identified that LSM14B is essential component of P-body-like granules and MARDO in mouse oocytes and required for the primordial follicle assembly and meiotic progression. Furthermore, we revealed that loss of LSM14B causes mRNA reduction in GO and disrupts maternal mRNA accumulation and translation in FGO. We propose that LSM14B is an important maternal factor and drives the maternal mRNA storage and stabilization during mouse oocyte growth.
In humans and mice, RNA transcription stops toward the end of oocyte growth and resumes after zygotic genome activation, during which stored maternal RNAs are used to make new proteins [49, 50]. Maternal mRNAs are thought to be stored in phase-separated membraneless compartments during oocyte growth and their stable storage is essential for successful meiosis maturation and early embryonic development [36, 51]. To date, membraneless RNA storage compartments have been partial characterized, and examples of such compartments include P granules and polar granules in non-mammalian oocytes [9, 47], as well as P-body-like granules and MARDO in mammalian early oocytes and FGO, respectively [13, 34]. Nevertheless, the function of the core component of the membraneless compartment has still not been fully dissected. We found that LSM14B co-localizes with DDX6 to P-body-like granules in germ cell. Recent studies demonstrated that the integrity of P-body-like granules is required for assembly of primordial follicles [13]. Consistent with this, the absence of Lsm14b leads to the dissolution and disappearance of P-body-like granules, consequently impacting the primordial follicles formation. Our results revealed that more than 50% of transcripts were found to be down-regulated in Lsm14b KO NGO. These data indicate that many mRNA are stored in the P-body-like granules and that disruption of P-body-like granules causes mRNA reduction. Interestingly, the down-regulated mRNAs in Lsm14b KO germ cells were associated with processes related to “mitochondrion organization” and “oxidative phosphorylation.” It is demonstrated that depletion of ZAR1 disrupts MARDO formation and mitochondrial clustering [34]. Given that LSM14B serves as a core component of the MARDO, further study should investigate the potential effects of LSM14B deficiency on the morphology and distribution of mitochondria in mammalian oocytes.
Oocyte meiotic maturation and early embryonic development rely on maternal mRNAs stored in FGO [52]. Previous studies have shown that loss of YBX2 in mouse oocytes leads to developmental retardation and failure of normal maturation of oocytes [35]. Notably, YBX2 is a RNA-binding protein, and YBX2 deficiency mainly reduces mRNA stability, thereby disrupting maternal mRNA accumulation [35]. The other study confirmed that ZAR1/2 are required for oocyte meiotic maturation by regulating the maternal transcriptome [6]. Our study showed that loss of LSM14B caused a large number of dysregulated maternal mRNA and meiotic defects. In addition, some of the aberrant transcripts in Lsm14b KO FGO were associated with “cell cycle arrest” and “embryo development,” However, LSM14B acting as an RNA-binding protein to bind which maternal mRNA needs to be explored in mammalian oocytes. It is demonstrated that the deficiency of ZAR1/2 in FGO impairs the 3’-UTR activation of maternal mRNA, thereby affecting the translation of maternal mRNA and causing oocyte meiotic defects [6]. In addition, previous study confirmed that maternal mRNAs stored in the MARDO are translationally repressed [34]. They also found that tdPCP-LSM14B also represses translation. In the present study, transcripts associated with ribosomes and translation are suppressed in Lsm14b KO FGO. Protein mass spectrometry analysis of FGO revealed that deletion of LSM14B alters the oocyte proteome. Collectively, these data indicate that LSM14B is essential for translation in mammalian oocyte.
Low levels of MPF activity are one of the causes of PN formation and meiotic defects [45]. Our observation that the Lsm14b KO oocytes failed to assemble the MII spindle after extrusion the PB1, but instead entered the interphase and formed PN. Furthermore, WB analysis of the p-CDK1 revealed that the levels of p-CDK1 are significantly reduced in Lsm14b KO MII oocytes. Likewise, Eml1 knockdown (KD) impairs MPF activity around the time of oocyte resumption, ultimately leading to the formation of PN [45]. Similarly, Mastl is required for the rapid rise of CDK1 activity that is needed for the entry into MII stage, and Mastl-null oocytes reassembled a nuclear structure containing decondensed chromatin and failed to enter MII because they maintain high levels of p-CDK1 [44]. It is therefore rational to believe that the absence of LSM14B destroyed the activity of MPF at optimal levels responsible for PN formation. This inference is further buttressed by the observation that PD166285, a WEE1/2 kinases inhibitor, effectively prevented the CDK1 (Y15) phosphorylation and the formation of PN in the Lsm14b KO oocytes. It is not clear how LSM14B may regulate the activity of MPF in oocytes. It could be that loss of LSM14B disrupts maternal mRNA stability and expression in GV oocyte. Nevertheless, the role of LSM14B during oocyte meiotic maturation is unclear. Whether LSM14B is essential for timely maternal mRNA degradation remains to be explored in mammalian oocytes.
Data availability
The FGO proteome and P7 ovaries Co-IP-MS data have been deposited to the integrated proteome resources (iProX) and the dataset identifiers PXD040510 and PXD040513, respectively. The scRNA-seq and RNA-seq data have been deposited in the Genome Sequence Archive under accession CRA009935 and CRA010004, respectively. All data needed to evaluate the conclusions in this work are present in the main text or the supplementary materials. The R code using for data processing is publicly available at GitHub at https://github.com/ZZlab412/Lsm14b-KO-mice-sequencing-data-analysis-script.
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Acknowledgements
The authors would like to thank Qing-Yuan Sun and Wei Shen for critical reading of this manuscript and contribution to the discussions.
Funding
This study was supported by the Inner Mongolia Autonomous Region Open Competition Projects (2022JBGS0024 to TZ and YBL), the National Natural Science Foundation of China (32260180 to TZ; 32260181 to YZ; 32000582 to TZ), the Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region (NJYT23013 to YZ), the Central Government Guides Local Science and Technology Development Fund Projects (2022ZY0188 to ZT), and the Science and Technology Major Project of Inner Mongolia Autonomous Region of China to the State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock (2019ZD031, 2021ZD0048).
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TZ and YZ designed and supervised the study; LYS, WXL, YT, HTF, FGL, AL, and WJL performed the experiments and analyzed the data; TZ and YZ wrote the manuscript; YBL, QYS, WS, and TZ contributed to the manuscript revision and discussion. All the authors read the manuscript and approved the final manuscript.
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Shan, LY., Tian, Y., Liu, WX. et al. LSM14B controls oocyte mRNA storage and stability to ensure female fertility. Cell. Mol. Life Sci. 80, 247 (2023). https://doi.org/10.1007/s00018-023-04898-2
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DOI: https://doi.org/10.1007/s00018-023-04898-2