Erratum to: Processing of joint molecule intermediates by structure-selective endonucleases during homologous recombination in eukaryotes

Erratum to: Chromosoma

DOI 10.1007/s00412-010-0304-7

The original version of this article unfortunately contained some mistakes.

In Figure 1, the legend contains a mistake. The corrected figure legend is given below.

Multiple DNA repair pathways are employed during doublestrand break repair. After DNA DSB formation, the broken ends can be religated using minimal nucleotide homology through nonhomologous end joining (NHEJ). Alternatively, 5′ ends are resected to expose single-stranded DNA favoring alternate routes of DSB repair. The presence of direct DNA sequence repeats may provide homologous regions that can anneal to form a contiguous chromosome in a process called single-strand annealing (SSA). Heterologous 3′ flaps are removed by the XPF endonuclease aided by Slx4 and Saw1. Alternatively, Rad51-dependent homology search and strand invasion forms a displacement loop (D-loop) to prime DNA synthesis from the 3′-OH end of the broken chromosome on an intact template. Extension of the D-loop and subsequent D-loop disruption and reannealing to the second end repairs the break via synthesis-dependent strand annealing (SDSA) resulting in noncrossover (NCO) products (Resnick 1976). Formation of an intact replication fork leads to the continuous extension of the D-loop to the end of the chromosome, defining the break-induced replication (BIR) pathway and resulting in loss of heterozygosity (LOH) (Malkova et al. 1996). The elongated D-loop forms a junction, where branch migration may lead to the formation of a single Holliday junction (sHJ). In the event of second-end capture, the displaced strand of the D-loop anneals to the other resected 3′ strand forming first two nicked Holliday junctions (nHJ) and after ligation a double Holliday junction (dHJ) (Szostak et al. 1983). Double HJs can be dissolved by the combined activities of a DNA motor protein (S. cerevisiae Sgs1 or human BLM) and a type IA topoisomerase into NCO products (Wu and Hickson 2003; Cejka et al. 2010) or resolved by coordinated endonuclease cleavage into CO or NCO products (Szostak et al. 1983). Single HJs require resolution by anuclease and cannot be processed by a dissolution mechanism like dHJs.

Table 1 contains 4 typographical errors, see the corrected table below.

Table 1 Structure-selective endonucleases exhibit an array of species specific differences. Organized by a single identifying subunit, eukaryotic homologs are listed with species designation. Information on their known binding partners, respective endonuclease superfamily, and mechanistic pathway involvement are provided

On page 117, left column Line 44: replace ‘strong a preference’ with ‘a strong preference’. The correct sentence must read

There is a wide consensus that Mus81–Mms4/EME1 endonucleases from all organisms studied have a strong preference for nicked junction substrates over classical HJ substrates.

On page 117, right column Line 34 replace ‘Rad2/XPF’ with ‘Rad2/XPG’

On page 117, right column Line 37: Replace ‘a 381-amino-acid C-terminal truncation’ by: ‘an approximately 60 kDa N-terminal fragment.’

The correct sentence must read

The RuvC-like activity in human cells was determined to be dependent on the catalytic activity of the Rad2/XPG endonuclease HsGEN1 (Ip et al. 2008). HsGEN1 was isolated by column fractionation and identified by mass spectroscopy as an approximately 60 kDa N-terminal fragment.