Abstract
In the genus Pseudomonas, zinc homeostasis is mediated by a complete set of import and export systems, whose expression is precisely controlled by three transcriptional regulators: Zur, CzcR and CadR. In this review, we describe in detail our current knowledge of these systems, their regulation, and the biological significance of zinc homeostasis, taking Pseudomonas aeruginosa as our paradigm. Moreover, significant parts of this overview are dedicated to highlight interactions and cross-regulations between zinc and copper import/export systems, and to shed light, through a review of the literature and comparative genomics, on differences in gene complement and function across the whole Pseudomonas genus. The impact and importance of zinc homeostasis in Pseudomonas and beyond will be discussed throughout this review.
Graphical abstract
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Zinc, which occurs as the divalent Zn2+ inside cells, is a trace element essential for life. It is a cofactor of many enzymes and stabilizes the structure of even more proteins, including transcription factors. In prokaryotes, zinc binds to 5 to 6% of proteins, playing both structural and functional roles (Chandrangsu et al. 2017). However, when present in excess, zinc becomes toxic mainly because it binds to metalloproteins normally associated with other metals from the Irving-Williams series and compromises their function: this phenomenon is called mismetallation (Foster et al. 2014). As a consequence, intracellular zinc concentrations have to be finely regulated at all times. This is done, in all living beings, through zinc homeostasis systems which control the uptake and release of the metal and maintain adequate cellular zinc concentrations. In bacteria, the cytoplasmic level of zinc stabilizes between 10–3 and 10–4 M, making it the second most abundant metal after iron; however, because zinc is largely complexed by its ligands, the free ions concentration is very low, ranging from 10–12 to 10–14 M (Chandrangsu et al. 2017).
Because bacteria are crippled by either lack or excess of zinc, many eukaryotes have evolved means to manipulate local zinc concentrations to counter bacterial infections. In mammals, zinc, like other essential metals such as iron and manganese, is sequestered from the serum and interstitial liquid to block pathogen development in a process called nutritional immunity (Kehl-Fie and Skaar 2010). Conversely, in some compartments of eukaryotic cells, zinc concentrations can reach inhibitory or even toxic levels (Djoko et al. 2015). In mammalian phagocytic cells, toxic concentrations of zinc are transferred from the cytoplasm to the phagosomes containing engulfed bacteria through the ZnT transporter family (Eide 2006; Huang and Tepaamorndech 2013). In the amoeba Dictyostelium discoideum, zincosomes, a particular type of endosome containing high levels of zinc, fuse with the phagosome to neutralize bacteria. The intracellular survival of bacteria therefore correlates with their capacity to export zinc: for instance, an Escherichia coli strain deficient in its primary zinc efflux system (ZntA) has been shown to be killed faster than the wild type in D. discoideum (Barisch et al. 2018). Plants also protect themselves against herbivores and phytopathogens by accumulating zinc in their tissues. This is well-illustrated in members of the Brassicaceae, such as Noccaea caerulescens, which can accumulate zinc up to 14 g/kg of dry weight. Such a concentration contributes to inhibit the growth of most pathogens. Typically, the metals are complexed by specialized molecules present in epidermal vacuoles and released during the destruction of these cells by the pathogen (Broadley et al. 2007). As a consequence, zinc plays a major role in host–pathogen interactions across the eukaryotic world (Hood and Skaar 2012; Lopez and Skaar 2018).
Some bacteria, however, have evolved efficient strategies to deal with extreme zinc concentrations and strong zinc fluctuations. Such bacteria have often been isolated from soil, which offers a wide range of zinc availability. For example, sandy soils in central Africa and southern Asia are severely deficient in zinc and thus force endemic plants and microorganisms to use strategies in order to recover the little metal available (Alloway 2009). Conversely, more and more sites contaminated with heavy metals, including zinc, are enriched in microorganisms which are highly metal-resistant (Diaz-Ravina and Baath 1996; Moffett et al. 2003).
Bacteria of the genus Pseudomonas are particularly robust when it comes to withstand extreme concentrations of zinc and other metals. They possess a complete arsenal of efflux systems that actively expel cytoplasmic or periplasmic metals out of the cell. Some species are also well-armed to recover metals and thrive under zinc-limited conditions. It is therefore not surprising to find among Pseudomonas species some major plant and animal pathogens able to withstand nutritional immunity and to evade phagocytosis. This makes the study of zinc homeostasis in these organisms of dual interest, both environmental and clinical.
Pseudomonas aeruginosa is a major opportunistic pathogen listed in 2017 as priority 1 for the discovery of antibacterial treatments according to the WHO (Tacconelli et al. 2018) WHO. Its ability to colonize its host heavily relies on its quick and effective responses to extreme zinc fluctuations. Accordingly, P. aeruginosa encodes versions of most zinc transport systems described in Gram-negative bacteria. Some of these zinc transporters have been shown to directly modulate virulence and antibiotic resistance (Perron et al. 2004; Dieppois et al. 2012; Ellison et al. 2013; D'Orazio et al. 2015). In addition, when exposed to Zn excess, P. aeruginosa becomes more virulent, causing gastrointestinal dysfunction and illness in a mouse model (Wu et al. 2021). For all these reasons, zinc homeostasis has been extensively studied in this species. While P. aeruginosa remains our the central reference model, the present review will broaden its scope to cover zinc homeostasis in the entire Pseudomonas genus.
ZINC uptake
From outside to the periplasm
When available in the environment, elemental zinc is able to cross the outer membrane by passive or facilitated diffusion using ion channels (Cerasi et al. 2013). Active acquisition systems however are required when very low concentrations of the trace element are present in the surrounding environment. Active transport of zinc through outer membrane channels is driven by TonB-ExbB-ExbD complexes sitting in the cytoplasmic membrane; energy is provided through the H+ gradient and relayed to the outer membrane channel by the proline-rich spacer of TonB proteins (Stork et al. 2010; Krewulak and Vogel 2011), (Fig. 1). Four TonB-dependent receptors are involved in zinc import in P. aeruginosa (Pederick et al. 2015). ZnuD, for zinc uptake component D, and PA1922 appear to be responsible for the import of free Zn2+, while ZrmA (CntO), which is part of the pseudopaline system described below, and PA2911 seem to be specific for a chelated form of the metal.
From the periplasm to the cytoplasm
In prokaryotes, the ATP-binding cassette (ABC-)permease ZnuABC is the most common mechanism allowing zinc to cross the cytoplasmic membrane (Fig. 1). First identified in E. coli (Patzer and Hantke 1998), this transport system is, based on the number of transmembrane helices of ZnuA a member of the type-I ABC transporter family (Davies et al. 2021). The transport of Zn2+ involves a periplasmic solute-binding protein (SBP), ZnuA, which captures the zinc ion and delivers it to the ZnuB transmembrane channel; the energy required for the translocation is provided by the ATPase ZnuC. The system operates according to a ZnuAB2C2 stoichiometry. The genes coding for the ZnuA, ZnuB and ZnuC proteins show 60%, 60% and 61% identity with their P. aeruginosa counterparts (Ellison et al. 2013).
In P. aeruginosa, ZnuA harbors a histidine-rich region characteristic of Zn2+ SBP, where residues H60, H140 and H204 appear to be responsible for binding to a Zn2+ ion (Site a in Fig. 2A). An in silico reconstitution of the structure, shown in Fig. 2, reveals the typical A-I fold cluster resulting in a rigid structure (Pederick et al. 2015; Fukamizo et al. 2019). SBP metal specificity is sometimes difficult to determine. For instance, the P. protegens Pf-5 ABC transporter PFL_0643-0645 is annotated as a FeCT family iron-chelated transport system. However, the PFL_0644 SBP is overexpressed under zinc-limiting conditions and a Zur-box has been predicted on its promoter (Lim et al, 2013). Moreover, no substantial change in transcription has been observed under iron starvation versus iron excess conditions (Lim et al. 2012). Finally, considering that PA4045 (BtuF) and PA2913, the closest orthologues of PFL_0644 in P. aeruginosa (according to Pseudomonas.com), are predicted to be a chelated cobalt (vitamin B12) and a chelated zinc transporter respectively (Borths et al. 2002; Pederick et al. 2015), we propose that PFL_0643-0645 might be an ABC transporter specific for complexed forms of zinc or cobalt. In P. putida, two ZnuABC systems are encoded in different operons, one of which may also be involved in the import of manganese (Canovas et al. 2003).
Besides ZnuABC, P. aeruginosa encodes two additional, poorly characterized, zinc ABC transporters (Pederick et al. 2015). The PA2914 system uses PA2913 as SBP, which falls in cluster A-II and is better suited to the transport of chelated ions (Pederick et al. 2015; Fukamizo et al. 2019). A recent study showed that, under conditions of zinc scarcity, the PA4063-PA4066 system was the ABC transporter with highest expression (Ducret et al. 2021). Intriguingly, this system has two probable SBPs: PA4063 and PA4066. Although these proteins exhibit a signal sequence that localizes them in the periplasmic space, they do not have a recognizable SBP structure. Moreover, only PA4063 contains a histidine stretch and therefore would be able to bind zinc (Pederick et al. 2015). This was recently confirmed by its crystallographic characterization that reveals two low-affinity zinc binding sites (Fig. 2B, (Fiorillo et al. 2021)). Additional experiments are needed to investigate the relative importance of these three systems in case of Zn deficiency.
Another type of system involved in zinc uptake is the P-type ATPase, a superfamily of transporters that translocate transition metals across the cytoplasmic membrane by hydrolyzing ATP. These systems usually act as efflux pumps. Nevertheless, in P. aeruginosa, overexpression of the P-type ATPase HmtA (for heavy metal transporter A) induces a hypersensitivity to zinc and copper and increased intracellular concentration of these metals, suggesting that the metal might be transported from the periplasm to the cytoplasm (Lewinson et al. 2009). Further analyses are needed to confirm this unusual characteristic. Moreover, it has been shown to be induced in a ΔznuA mutant (Pederick et al. 2015), suggesting an involvement in the zinc uptake pathway. Surprisingly, this transporter is the only known import system which is not repressed by zinc excess (Ducret et al. 2021). Thus, it would act as a safety mechanism in the event of sudden zinc deficiency or could simply be expressed for its role in copper entry.
Finally, zinc uptake in P. aeruginosa can also be driven by the ZrmABCD system, which involves a staphylopine-like molecule called pseudopaline (Mastropasqua et al. 2017). This zincophore is synthesized in the cytoplasm by the ZrmB (CntL) and ZrmC (CntM) enzymes; it crosses the cytoplasmic membrane via the EAM-like transporter ZrmD (CntI) (McFarlane and Lamb 2017) and is exported to the extracellular space through the MexAB-OprM efflux pump (Fig. 1) (Gomez et al. 2021). Pseudopaline bound to extracellular zinc or nickel ions is then reinternalized into the periplasmic space by the TonB-dependent transporter ZrmA (CntO) (Lhospice et al. 2017; Mastropasqua et al. 2017). The way the complex transits back to the cytoplasm remains unknown for the moment; but recent data suggests that pseudopaline is modified in the periplasm and probably releases the metal in this compartment (Gomez et al. 2021).
Nicotianamine-like metallophores like pseudopaline probably appeared early in evolution (Laffont and Arnoux 2020). Under the anoxic conditions of primitive Earth, which allowed for high concentrations of zinc, manganese and phosphate ions in solution, metal chelation could have helped with metal resistance (Laffont and Arnoux 2020). To cope with metal deprivation resulting from later oxidation events, metallophores could have then neofunctionalized in metal acquisition. They might only have been preserved in microorganisms evolving in metal-scarce conditions, as found within eukaryotic hosts or in sandy soils. Interestingly, the pseudopaline system is almost exclusively conserved in P. aeruginosa; typical environmental species like P. fluorescens or P. stutzeri do not present any close homolog of the pseudopaline synthesis genes (see below).
Zinc recycling
Zinc is used as a cofactor in 5 to 6% of the bacterial proteome (Andreini et al. 2006). This represents a significant stock of metal that can be mobilized by the cell under limiting conditions. “Zinc recycling,” also referred to as “zinc sparing” or “zinc economy” (Merchant and Helmann 2012), is a process proposed to increase intracellular zinc availability for essential functions: it involves replacing some zinc-binding proteins by paralogs which do not require zinc. These paralogs have lost their cysteine-containing Zn2+-binding domain; they are therefore referred to C − versions by contrast with the zinc-dependent C + family of proteins. C − paralogs are usually repressed under zinc-replete conditions but are expressed and can functionally replace their C + paralogs during metal starvation. In P. aeruginosa, C − paralogs are often grouped in operons. For instance, the PA3601-PA3600 transcription unit encodes for the C − ribosomal proteins RpmE2 and RpmJ2 respectively and the PA5536-PA5534 unit encodes for the transcriptional factor dksA2, involved in the stringent response, and genes belonging to the COG0523 family. Members of this protein family have a number of functions, including cobalamin biosynthesis, GTPases or metallochaperones and those aimed at sparing inadequate cellular zinc distribution (Crouzet et al. 1991; Haas et al. 2009; Jordan et al. 2019). For instance, the COG0523 G3E protein is a GTP-dependent metallochaperone involved in the maturation of metal-containing enzymes that are typically upregulated under conditions of zinc starvation (Haas et al. 2009; Capdevila et al. 2017). Another set of genes involved in the production of zinc-independent paralog enzymes, PA5539-PA5541, encodes for the GTP hydrolase FolE2 (PA5539), a putative γ-carbonic dehydratase (PA5540), and the dihydroorotase PyrC2 (PA5541) (Pederick et al. 2015). Similar clusters encoding non-zinc-requiring paralogs have been identified in P. protegens (Lim et al. 2013).
Replacement of C + by C − paralogs has been proposed to foster metal release when zinc availability becomes critical (Akanuma et al. 2006). This hypothesis is however controversial. For example, in E. coli, the ratio between RpmE and RpmJ and their respective C − paralogs has a direct impact on translation, suggesting a divergent role. Moreover, RpmE2 and RpmJ2 appeared to be expressed in late exponential growth phase, even in the presence of zinc (Lilleorg et al. 2020). This was also observed in P. protegens Pf-5, in which qRT-PCR revealed that expression of rpmE and rpmJ did not change under zinc-limitation (Lim et al. 2013). Similarly, in P. aeruginosa, several C + and C − paralogs are co-expressed even in metal-poor environments (Ducret et al. 2021). In this bacterium however, DksA2 and PyrC2 were clearly able to complement the deletion of their respective paralog and are therefore considered as backup copies induced in response to zinc deprivation (Brichta et al. 2004; Blaby-Haas et al. 2011).
ZINC export
Export machinery
Three types of transporters are involved in zinc export (Fig. 3). The CzcCBA RND efflux pump is by far the most effective, according to the level of resistance it confers. This complex belongs to the Heavy Metal Efflux (HME) Resistance Nodulation Division (RND) superfamily (Mergeay et al. 1985; Hassan et al. 1999); it was initially discovered and then studied in depth in Cupriavidus metallidurans, a bacterium known for its high resistance to heavy metals. CzcCBA is a tripartite complex with a CzC3B6A3 stoichiometry (Fig. 3). CzcC makes a beta barrel channel across the outer membrane; the RND family protein CzcA spans the cytoplasmic membrane with twelve alpha helices and the membrane fusion protein CzcB forms a ring that stabilizes the contact between CzcA and CzcC. In the presence of zinc or cadmium excess, the CzcCBA machinery utilizes the proton motive force to actively expel zinc, cadmium, and cobalt from the cell (Goldberg et al. 1999). Two mechanisms of metal export have been proposed. In the first model, zinc transits trough the periplasm: it is transferred to the metal binding site of CzcA via CzcB and then expelled through CzcC. This model seems currently the most likely. The second route allows zinc to be pumped out of the cytoplasm and ejected directly in the extracellular medium without releasing it into the periplasm (Kim et al. 2011). In some Pseudomonas species, this system is supplemented by accessory proteins. In P. stutzeri RCH2 for example, an additional periplasmic protein CzcI may act as a metal ion chaperone, facilitating the expulsion of periplasmic zinc by delivering it to CzcB (Vaccaro et al. 2016). The potential cytoplasmic membrane CzcN in P. putida KT2440 is another example of CzcCBA partner (Canovas et al. 2003). Interestingly, the genome of this bacterium encodes a doublet of the czcCBA operon and five additional czcA genes although only the CzcA1 and CzcA2 are close enough to the Cupriavidus CzcA to confer zinc resistance (Canovas et al. 2003). Moreover, the second copy of the czc operon (czc2) appears to serve as a backup since it is expressed only in a czc1-deficient background (Mumm et al. 2016). In C. metallidurans, an accessory protein called CzcE is localized in the periplasm; it is induced by zinc, but yet, was only shown to play a role in copper homeostasis, binding two copper ions (I or II) as a dimer (Zoropogui et al. 2008). The protein encoded by PA2807 in P. aeruginosa is a homolog of CzcE (Ducret et al. 2021), but is located in a copper cluster and co-transcribed with ptrA and queF (Quintana et al. 2017). This cluster is known to be part of the copper response two-component system CopRS regulon. Interestingly, this operon has also been shown to respond to zinc excess thanks to the two-component system CzcRS described below (Ducret et al. 2021). Therefore, induction of the ptrA-PA2807-queF operon occurs via two different two-component systems, depending on the inducing metal.
The Cation Diffusion Facilitator (CDF, Fig. 3) is the last known export system in P. aeruginosa. First described in 1997, CDFs were primarily thought to be involved in metal tolerance, functioning as proton antiporters to expel divalent cations across the cytoplasmic membrane (Paulsen and Saier 1997; Kolaj-Robin et al. 2015). Indeed, in some bacteria, CzcD and YiiP, which occur as homodimers or heterodimers, are key players in zinc homeostasis (Anton et al. 1999; Wei and Fu 2005). Structural analyses show that CDFs are composed of six transmembrane segments and a cytoplasmic C-terminal domain, important for transport regulation, but also for dimerization. Four zinc binding sites have been identified within a conserved “Site A” located in transmembrane helices 2 and 5, indispensable for transport (Paulsen and Saier 1997; Kolaj-Robin et al. 2015). In P. aeruginosa, CDFs are not essential for zinc resistance but may play a role in membrane integrity (Salusso and Raimunda 2017). In agreement with this, constitutive expression of CzcD is observed after addition of zinc, with only a weak and late induction (Ducret et al. 2020, 2021). Multiple copies of these transporters are found in the genomes of several Pseudomonas species. The presence of paralogs is likely the result of horizontal acquisitions or duplications and probably supports improved resistance to transition metals (Canovas et al. 2003; von Rozycki and Nies 2009).
Entrap zinc to survive?
We cannot conclude without considering the metallothioneins, a family of small proteins involved in sequestering excess metals in the cytoplasm. The first of these proteins identified in bacteria, SmtA, was discovered in 1979 in the cyanobacterium Synechococcus elongatus as a significant player in zinc detoxification (Olafson et al. 1979). To date, very few of these proteins have been described. These cytoplasmic proteins, rich in cysteine residues, are capable of scavenging a broad spectrum of essential and non-essential metals, forming metal-thiolate clusters. Metallothioneins can thus finely buffer the cytoplasm (Chatterjee et al. 2020). In addition, the numerous cysteine residues confer better resistance against oxidative stress by scavenging reactive oxygen and nitrogen species (Spahl et al. 2003; Zeitoun-Ghandour et al. 2011). In Pseudomonas however, a role for these proteins in zinc homeostasis has not yet been demonstrated. Two metallothioneins have been described, one in P. fluorescens Q2-87 and another in P. aeruginosa; however, both are expressed mainly in late stationary phase (after 24 to 48 h of growth) in planktonic cells or biofilms, and seem to provide a fitness benefit for long-term survival (Habjanic et al. 2018, 2020).
Regulation of zinc homeostasis
Regulation of zinc homeostasis under zinc limitation or sufficiency
The expression of zinc uptake systems and C − paralog proteins is transcriptionally regulated by the Zinc Uptake Regulator, Zur (Fig. 4). This transcriptional regulator belongs to the FUR (Ferric Uptake Regulator) family (Fillat 2014) and is widely conserved in bacteria, including proteobacteria (Panina et al. 2003). Like other allosteric regulators, Zur changes its DNA binding characteristics upon binding free cytoplasmic zinc (Ellison et al. 2013; Gonzalez et al. 2019). It possesses a very strong affinity for Zn with a constant of 6.4 × 10–13 M (Osman et al. 2015) that enables a graded response based on the concentration of the metal, allowing for a hierarchical regulation of targets. In Apo-Zur, only the structural site, located in the C-terminal region (C-site), is generally bound by zinc; the protein then forms a dimer (Zur2:Zn2) unable to bind DNA. When zinc concentration increases, the regulator is found in an intermediate Zur2:Zn3 state, in which the regulatory M-site of only one Zur per dimer is occupied by zinc; this conformation allows the dimer to bind DNA with low affinity. At higher zinc concentrations, the two M-sites of the dimer are occupied by zinc and this Zur2:Zn4 conformation leads to strong DNA binding activity (Shin and Helmann 2016). When bound to DNA, Zur usually functions as a repressor preventing the RNA polymerase from initiating transcription. In P. aeruginosa, the Zur protein, formerly known as NP20, has been characterized (Ellison et al. 2013). The Zur DNA binding site (Zur box) is defined as a 17 bp palindromic motif that often overlaps with the − 10 of target promoters. With the exception of the hmtA gene, Zur was found to bind promoters of all genes involved in zinc uptake, but also promoters of C − paralog proteins, in presence of zinc excess (Ducret et al. 2021). In several bacteria, including Xanthomonas campestris, Streptomyces coelicolor and Caulobacter crescentus, Zur can also act as an activator of zinc efflux genes (Huang et al. 2008; Mazzon et al. 2014; Choi et al. 2017). The localization of the Zur boxes on the promoter and the ability of the protein to multimerize at high zinc concentrations seem to determine whether the regulator functions as a repressor or an activator. This highlights the contribution of Zur to the adaptability of bacteria under zinc scarcity or excess.
Zinc homeostasis regulation under zinc excess
The high adaptability of the Pseudomonas genus is supported by numerous two-component systems (TCS) that allow a rapid and targeted response to environmental stimuli (Rodrigue et al. 2000). The CzcCBA efflux pump, for instance, is regulated by the CzcRS TCS (Fig. 4). CzcS is a cytoplasmic membrane-located sensor protein capable of detecting an excess of periplasmic cadmium or zinc, with an affinity of 1.7 × 10–6 M (Hassan et al. 1999; Perron et al. 2004; Wang et al. 2017). The ligand induces dimerization and autophosphorylation of the sensor on a conserved histidine residue. In this conformation, it activates the CzcR regulator by transferring its phosphoryl group to the regulatory aspartate residue. In turn, this protein directly activates both the transcription of the czcCBA operon and its own. The N-terminal periplasmic domain of the P. aeruginosa CzcS protein has been crystalized and its structure determined (Wang et al. 2017). Importantly, the structure shows that zinc is directly recognized by CzcS, without the need for additional periplasmic proteins. In P. aeruginosa, twelve response regulators of TCS might bind the intergenic region between czcRS and czcCBA forming CzcR as the “key downstream node of RR network” (Trouillon et al. 2021). Besides, CzcR exhibits a dual role: in addition to promoting zinc, cadmium, and cobalt resistance via the activation of the czcCBA efflux pump, it downregulates the expression of oprD by directly binding the promoter region. OprD is a porin involved in the entry of basic amino acids and carbapenem antibiotics (imipenem and meropenem), a very important class of antimicrobial compounds active against Pseudomonas (Dieppois et al. 2012). Its repression in the presence of zinc makes P. aeruginosa resistant to this family of antibiotics. Surprisingly, in P. stutzeri RCH2, CzcR has an opposite effect since it activates the expression of the OprD porin (Garber et al. 2018). Nevertheless, the biological reason for the zinc-dependent regulation of this porin remains a mystery. Many virulence factors are also controlled by CzcR, which can therefore be considered a global regulator connecting metal resistance with antibiotic resistance and virulence (Dieppois et al. 2012). Interestingly, some of these genes do not require zinc excess to be regulated, suggesting a basal CzcR activity. Recently, the CzcRS regulon was further expanded, since it appears that the zinc-mediated induction of czcD, along with that of ptrA and czcE, also hinges on this TCS (Ducret et al. 2021). Remarkably, two czcRS operons with an additional czcR3 have been identified in P. putida, all induced by zinc; this highlights the aptitude for sensor proteins to phosphorylate at least one or two different CzcR (Mumm et al. 2016).
In P. aeruginosa, proper activation of CzcRS requires the CadA exporter, a P-type ATPase regulated by CadR (ZntR) (Fig. 4). A dual role has been attributed to this transcriptional regulator: while it clearly acts as an inducer in the presence of zinc, it also appears to exert slight negative control in conditions of metal limitation (Ducret et al. 2020). The cadR gene is located just upstream of cadA, but transcribed in the opposite direction. It belongs to the MerR-like family and should bind between the − 35 and − 10 of target promoters (Brown et al. 2003). Under starvation conditions, CadR bends the DNA around the operator and prevents the RNA polymerase from initiating transcription. At high zinc concentrations, an allosteric change in the regulator bound to the metal, with an affinity constant of 3.2 × 10–12 M (Osman et al. 2015), relieves the DNA and allows the RNAP to start transcription.
The crossing pathways between zinc and copper homeostasis
The response to zinc is often intimately linked to the response to copper. In bacteria, the cross-resistance to zinc and copper could be linked to the presence of both metals in the phagosomes of macrophages or protozoa. Indeed, numerous examples of Zn-Cu coregulations have been described in both Gram-positive and Gram-negative bacteria. For instance in Enterococcus faecalis, a general transcriptional network responds to both metals (Latorre et al. 2015) and numerous efflux systems of Acinetobacter baumanii respond to both Zn and Cu (Hassan et al. 2017). In P. aeruginosa, the czcE and ptrA genes are located on a copper cluster. Deletion of these genes appears to increase copper and zinc sensitivity, definitively linking these proteins to the homeostasis of both metals (Elsen et al. 2011; Ducret et al. 2021). By contrast, CzcE does not affect copper tolerance in P. putida. In P. aeruginosa, copper also has a positive effect on zinc resistance. This increased resistance is the consequence of the direct interaction of CopR, the transcriptional regulator of the copper specific TCS, with the czcR promoter (Caille et al. 2007). In P. stutzeri, this interconnection goes further, since CzcR, CopR1 and CopR2 have almost the same gene targets. This might be explained by the presence of a very similar DNA binding site for the three regulators (Garber et al. 2018). Although a link exists between zinc and copper in different species, the nature of the interplay between the two can be very different. In P. stuzeri, for instance, zinc induces the expression of the copper responding TCS (CopRS) via CzcRS (Garber et al. 2018), unlike what has been shown in P. aeruginosa (Caille et al. 2007). Interestingly a zinc-copper overlap is also found under metal starvation conditions. For instance, HmtA imports exclusively zinc and copper, unlike the conventional P-type ATPases that are either specific for zinc-cadmium or for copper-silver (Lewinson et al. 2009). Another example is the cytoplasmic metallochaperone CopZ and the porin OprC, related to copper metabolism, that are highly downregulated when P. protegens Pf-5 is exposed to a limiting amount of zinc (Lim et al. 2013). Why resistance to Zn and Cu is co-regulated is unclear. However, the antibacterial effect of these metals and their co-localization in phagosomes strongly suggest that a coordinated response to Zn and Cu excess is advantageous (Djoko et al. 2015). Deciphering the molecular basis of this co-regulation would be key to improve our understanding of the role of metals in host–pathogen interactions.
Zinc homeostasis beyond P. aeruginosa
The data available on zinc homeostasis systems in P. aeruginosa is disproportionate compared to other Pseudomonas species. Although we have mentioned the few experimental studies covering parts of the system in P. protegens, P. putida, P. stutzeri, and P. syringae, an overview for the entire genus is still missing. To start closing the gap, we have surveyed the proteomes of 1254 sequenced Pseudomonas strains, available on the NCBI database, including about 300 strains not belonging to the aeruginosa group, using bioinformatics. Briefly, we looked for the best reciprocal protein blastp (v. 2.8.1 +) hit (Altschul et al. 1990, 1997) of all proteins known to be involved in zinc homeostasis in P. aeruginosa PAO1 in our genome set, retaining only reciprocal hits with an e-value < 0.01 and a query coverage of at least 75% (with the exception of CzcB, which has an N-terminal extension in PAO1); the requirement for a reciprocal hit and high query coverage ensured that the evalues were in general very low and that the likelihood of false positives was minimized. Strains had been clustered into large phylogenetic groups (“aeruginosa”, “chlororaphis-protegens”, “fluorescens”, “putida”, “stutzeri” and “syringae”) based on a tree built from an alignment of concatenated conserved ribosomal proteins using fasttree (Price et al. 2010). Table 1A presents the percentage of strains within each group encoding at least one possible homolog for each protein; Table 1B gives the median of the blast best hit evalues within each group and highlights empty cells and potential false positives.
The P1B (or heavy metal transporting)-ATPase CadA (or ZntA) is a second system involved in zinc resistance in P. aeruginosa (Fig. 3). The presence of a long histidine-rich tail might suggest that this protein is involved in the transport of cadmium (Arguello 2003), with a possible partial function in zinc and lead export from the cytoplasm to the periplasm (Ducret et al. 2020). As the first export system to be induced during a metal boost, CadA rapidly removes zinc excess from the cytoplasm. In addition, the zinc transferred to the periplasmic compartment is thought to play a major role in czcCBA induction (Ducret et al. 2020). P. putida KT2440 contains two functional cadA genes, one in the same genetic context as in P. aeruginosa, the other one located in the middle of the czc locus (Canovas et al. 2003; Leedjarv et al. 2008). Surprisingly, CadA was shown to be poorly conserved across the Pseudomonas stutzeri species (Table 1).
Two features clearly put P. aeruginosa apart within the Pseudomonas genus when it comes to zinc import: 1. the pseudopaline system does not seem to be present in any other group (with the exception of homologs of ZrmA, which belongs to a widespread group of TonB transporters, difficult to discriminate on a bioinformatic basis); 2. the PA1922-25 system, which is suspected to encode an nonchelated-zinc import machinery, does not have orthologs in most other Pseudomonas species. This suggests that P. aeruginosa has invested more than other species into alternative zinc import systems, which presumably makes it better equipped to face nutritional immunity in mammalian plasma than purely environmental strains. Likewise, the C − proteins RpmJ2 and RpmE2 seem to be mostly restricted to the aeruginosa group (with the interesting exception of members of the syringae group, which have a RpmE2 ortholog), while PyrC2 and FolE2 homologs are found more widely in environmental Pseudomonas strains. We speculate that C- ribosomal proteins could be particularly advantageous for pathogens, like P. aeruginosa and P. syringae, for which high growth rates under limited zinc concentrations might be selective. On the other hand, the homologs of the three ABC transporters, ZnuABC, PA4063-66, and, to a lesser extent, PA2911-14, may be ancestral to the Pseudomonas genus and appear to be conserved independently from environment or lifestyle. Interestingly, the TonB transporters ZnuD, PA1922, and PA2911, which are responsible for the import of metallic zinc through the external membrane, do not seem to be as conserved as the cytoplasmic membrane components; different groups seem to have preferentially retained different paralogs (ZnuD and PA2911 are present in the majority of putida and chlororaphis strains, while PA1922 is absent; by contrast, PA1922 is frequent in the stutzeri group, where PA2911 is underrepresented). This suggests that there is some redundancy among TonB transporters involved in zinc import and that different patterns of gain and loss may have prevailed in different phylogenetic groups or environments. Among the proteins involved in zinc export, the most conserved in Pseudomonas strains is the CDF YiiP; an ortholog of CzcD is found in most aeruginosa and chlororaphis strains, but in less than half of the other strains. The efflux pump CzcCBA is usually found together with the CzcRS two component system; these systems are very conserved in all groups except fluorescens and stutzeri, where proper orthologs are missing in about 50% of strains. This relatively patchy distribution of export systems suggests that zinc excess might not be as strong a selective force in some natural environments as it is during pathogenesis in animals. Finally, we looked for Zur boxes in the promoter region of the different homologs we found (Table 2). This data supports a rather high conservation of the Zur-dependent regulation for zur, zrmA, znuD, znuA, PA4063, rpmE2, and dksA2 (and following genes in the case of polycistronic RNAs); the regulation of PA4063 and dksA2 by Zur, however, seems to be lacking in specific groups (putida and syringae). This quite high stability of targets is consistent with the broad structural and functional conservation of Zur across Proteobacteria. The regulation by Zur of PA1922 and czcR on the other hand seems unique to P. aeruginosa.
Overall, it seems that, apart from the exceptions mentioned earlier, the zinc homeostasis systems present in P. aeruginosa are mostly representative of the genus. There is, however, a notable tendency for strains in the fluorescens and the stutzeri groups to lack some systems widespread elsewhere. These environmental bacteria might be less exposed to zinc fluctuations or rely on promiscuous systems involved in the homeostasis of other metals, like copper or cadmium.
Concluding remarks
The genus Pseudomonas includes bacteria with such versatility that they can be found in all environments, worldwide. All species in the genus possess a range of transporters and regulators supporting their survival and growth under both zinc scarcity and zinc excess. Although these systems are almost all represented within the genus, their number, their arrangement on the chromosome, and their effects on cell physiology seem to be species-specific. This is the case, for example, in zinc excess situations where the OprD porin is repressed in P. aeruginosa while it is overexpressed in P. stuzeri (Perron et al. 2004; Garber et al. 2018). The link between zinc and the expression of virulence factors in P. aeruginosa has stimulated the study of metal homeostasis in this pathogen. Unfortunately, the effects of the lack or excess of zinc in other species is poorly studied and deserves further investigations. With this in mind, future research in the field should focus on:
-
i.
The interconnection between the various transcriptional regulators involved in both zinc and copper resistance, and both the import and export of these metals. For example, the localization of Zur, the repressor of import systems, on the promoter of czcCBA involved in Zn export confers an additional level of complexity, which still remains to be deciphered.
-
ii.
The importance of Zn homeostasis should also be studied in plant pathogens, where studies are lacking in comparison to P. aeruginosa.
-
iii.
The biochemical characterization of Zn transport systems. Many of these systems are only described by homology, but their involvement in the transport of specific metals has not yet been clearly demonstrated.
These research directions could open the way to a renewed and more integrated understanding of metal homeostasis in the genus Pseudomonas.
References
Akanuma G, Nanamiya H, Natori Y, Nomura N, Kawamura F (2006) Liberation of zinc-containing L31 (RpmE) from ribosomes by its paralogous gene product, YtiA, in Bacillus subtilis. J Bacteriol 188:2715–2720
Alloway BJ (2009) Soil factors associated with zinc deficiency in crops and humans. Environ Geochem Health 31:537–548
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Andreini C, Banci L, Bertini I, Rosato A (2006) Zinc through the three domains of life. J Proteome Res 5:3173–3178
Anton A, Grosse C, Reissmann J, Pribyl T, Nies DH (1999) CzcD is a heavy metal ion transporter involved in regulation of heavy metal resistance in Ralstonia sp. strain CH34. J Bacteriol 181:6876–6881
Arguello JM (2003) Identification of ion-selectivity determinants in heavy-metal transport P1B-type ATPases. J Membr Biol 195:93–108
Barisch C, Kalinina V, Lefrancois LH, Appiah J, Lopez-Jimenez AT, Soldati T (2018) Localization of all four ZnT zinc transporters in dictyostelium and impact of ZntA and ZntB knockout on bacteria killing. J Cell Sci. https://doi.org/10.1242/jcs.222000
Blaby-Haas CE, Furman R, Rodionov DA, Artsimovitch I, de Crecy-Lagard V (2011) Role of a Zn-independent DksA in Zn homeostasis and stringent response. Mol Microbiol 79:700–715
Borths EL, Locher KP, Lee AT, Rees DC (2002) The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter. Proc Natl Acad Sci U S A 99:16642–16647
Brichta DM, Azad KN, Ralli P, O’Donovan GA (2004) Pseudomonas aeruginosa dihydroorotases: a tale of three pyrCs. Arch Microbiol 182:7–17
Broadley MR, White PJ, Hammond JP, Zelko I, Lux A (2007) Zinc in plants. New Phytol 173:677–702
Brown NL, Stoyanov JV, Kidd SP, Hobman JL (2003) The MerR family of transcriptional regulators. FEMS Microbiol Rev 27:145–163
Caille O, Rossier C, Perron K (2007) A copper-activated two-component system interacts with zinc and imipenem resistance in Pseudomonas aeruginosa. J Bacteriol 189:4561–4568
Canovas D, Cases I, de Lorenzo V (2003) Heavy metal tolerance and metal homeostasis in Pseudomonas putida as revealed by complete genome analysis. Environ Microbiol 5:1242–1256
Capdevila DA, Edmonds KA, Giedroc DP (2017) Metallochaperones and metalloregulation in bacteria. Essays Biochem 61:177–200
Cerasi M, Ammendola S, Battistoni A (2013) Competition for zinc binding in the host-pathogen interaction. Front Cell Infect Microbiol 3:108
Chandrangsu P, Rensing C, Helmann JD (2017) Metal homeostasis and resistance in bacteria. Nat Rev Microbiol 15:338–350
Chatterjee S, Kumari S, Rath S, Priyadarshanee M, Das S (2020) Diversity, structure and regulation of microbial metallothionein: metal resistance and possible applications in sequestration of toxic metals. Metallomics 12:1637–1655
Choi SH, Lee KL, Shin JH, Cho YB, Cha SS, Roe JH (2017) Zinc-dependent regulation of zinc import and export genes by Zur. Nat Commun 8:15812
Crouzet J, Levy-Schil S, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Debussche L, Thibaut D (1991) Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase. J Bacteriol 173:6074–6087
Davies JS, Currie MJ, Wright JD, Newton-Vesty MC, North RA, Mace PD, Allison JR, Dobson RCJ (2021) Selective nutrient transport in bacteria: multicomponent transporter systems reign supreme. Front Mol Biosci 8:699222
Diaz-Ravina M, Baath E (1996) Development of metal tolerance in soil bacterial communities exposed to experimentally increased metal levels. Appl Environ Microbiol 62:2970–2977
Dieppois G, Ducret V, Caille O, Perron K (2012) The transcriptional regulator CzcR modulates antibiotic resistance and quorum sensing in Pseudomonas aeruginosa. PLoS ONE 7:e38148
Djoko KY, Ong CL, Walker MJ, McEwan AG (2015) The Role of copper and zinc toxicity in innate immune defense against bacterial pathogens. J Biol Chem 290:18954–18961
D’Orazio M, Mastropasqua MC, Cerasi M, Pacello F, Consalvo A, Chirullo B, Mortensen B, Skaar EP, Ciavardelli D, Pasquali P et al (2015) The capability of Pseudomonas aeruginosa to recruit zinc under conditions of limited metal availability is affected by inactivation of the ZnuABC transporter. Metallomics 7:1023–1035
Ducret V, Gonzalez MR, Leoni S, Valentini M, Perron K (2020) The CzcCBA efflux system requires the CadA P-Type ATPase for Timely expression upon zinc excess in Pseudomonas aeruginosa. Front Microbiol 11:911
Ducret V, Abdou M, Goncalves Milho C, Leoni S, Martin-Pelaud O, Sandoz A, Segovia Campos I, Tercier-Waeber ML, Valentini M, Perron K (2021) Global analysis of the zinc homeostasis network in Pseudomonas aeruginosa and its gene expression dynamics. Front Microbiol 12:739988
Eide DJ (2006) Zinc transporters and the cellular trafficking of zinc. Biochem Biophys Acta 1763:711–722
Ellison ML, Farrow JM 3rd, Parrish W, Danell AS, Pesci EC (2013) The transcriptional regulator Np20 is the zinc uptake regulator in Pseudomonas aeruginosa. PLoS ONE 8:e75389
Elsen S, Ragno M, Attree I (2011) PtrA is a periplasmic protein involved in Cu tolerance in Pseudomonas aeruginosa. J Bacteriol 193:3376–3378
Fillat MF (2014) The FUR (ferric uptake regulator) superfamily: diversity and versatility of key transcriptional regulators. Arch Biochem Biophys 546:41–52
Fiorillo A, Battistoni A, Ammendola S, Secli V, Rinaldo S, Cutruzzola F, Demitri N, Ilari A (2021) Structure and metal-binding properties of PA4063, a novel player in periplasmic zinc trafficking by Pseudomonas aeruginosa. Acta Crystallogr D Struct Biol 77:1401–1410
Foster AW, Osman D, Robinson NJ (2014) Metal preferences and metallation. J Biol Chem 289:28095–28103
Fukamizo T, Kitaoku Y, Suginta W (2019) Periplasmic solute-binding proteins: structure classification and chitooligosaccharide recognition. Int J Biol Macromol 128:985–993
Garber ME, Rajeev L, Kazakov AE, Trinh J, Masuno D, Thompson MG, Kaplan N, Luk J, Novichkov PS, Mukhopadhyay A (2018) Multiple signaling systems target a core set of transition metal homeostasis genes using similar binding motifs. Mol Microbiol 107:704–717
Goldberg M, Pribyl T, Juhnke S, Nies DH (1999) Energetics and topology of CzcA, a cation/proton antiporter of the resistance-nodulation-cell division protein family. J Biol Chem 274:26065–26070
Gomez NO, Tetard A, Ouerdane L, Laffont C, Brutesco C, Ball G, Lobinski R, Denis Y, Plesiat P, Llanes C et al (2021) Involvement of the Pseudomonas aeruginosa MexAB-OprM efflux pump in the secretion of the metallophore pseudopaline. Mol Microbiol 115:84–98
Gonzalez MR, Ducret V, Leoni S, Perron K (2019) Pseudomonas aeruginosa zinc homeostasis: key issues for an opportunistic pathogen. Biochim Biophys Acta Gene Regul Mech 1862:722–733
Haas CE, Rodionov DA, Kropat J, Malasarn D, Merchant SS, de Crecy-Lagard V (2009) A subset of the diverse COG0523 family of putative metal chaperones is linked to zinc homeostasis in all kingdoms of life. BMC Genomics 10:470
Habjanic J, Zerbe O, Freisinger E (2018) A histidine-rich Pseudomonas metallothionein with a disordered tail displays higher binding capacity for cadmium than zinc. Metallomics 10:1415–1429
Habjanic J, Mathew A, Eberl L, Freisinger E (2020) Deciphering the enigmatic function of Pseudomonas metallothioneins. Front Microbiol 11:1709
Hassan MT, van der Lelie D, Springael D, Romling U, Ahmed N, Mergeay M (1999) Identification of a gene cluster, czr, involved in cadmium and zinc resistance in Pseudomonas aeruginosa. Gene 238:417–425
Hassan KA, Pederick VG, Elbourne LD, Paulsen IT, Paton JC, McDevitt CA, Eijkelkamp BA (2017) Zinc stress induces copper depletion in Acinetobacter baumannii. BMC Microbiol 17:59
Hood MI, Skaar EP (2012) Nutritional immunity: transition metals at the pathogen-host interface. Nat Rev Microbiol 10:525–537
Huang L, Tepaamorndech S (2013) The SLC30 family of zinc transporters - a review of current understanding of their biological and pathophysiological roles. Mol Aspects Med 34:548–560
Huang DL, Tang DJ, Liao Q, Li HC, Chen Q, He YQ, Feng JX, Jiang BL, Lu GT, Chen B et al (2008) The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters. Nucleic Acids Res 36:4295–4309
Jordan MR, Wang J, Weiss A, Skaar EP, Capdevila DA, Giedroc DP (2019) Mechanistic insights into the metal-dependent activation of Zn(II)-Dependent Metallochaperones. Inorg Chem 58:13661–13672
Kehl-Fie TE, Skaar EP (2010) Nutritional immunity beyond iron: a role for manganese and zinc. Curr Opin Chem Biol 14:218–224
Kim EH, Nies DH, McEvoy MM, Rensing C (2011) Switch or funnel: how RND-type transport systems control periplasmic metal homeostasis. J Bacteriol 193:2381–2387
Kolaj-Robin O, Russell D, Hayes KA, Pembroke JT, Soulimane T (2015) Cation diffusion facilitator family: structure and function. FEBS Lett 589:1283–1295
Krewulak KD, Vogel HJ (2011) TonB or not TonB: is that the question? Biochem Cell Biol 89:87–97
Laffont C, Arnoux P (2020) The ancient roots of nicotianamine: diversity, role, regulation and evolution of nicotianamine-like metallophores. Metallomics 12:1480–1493
Latorre M, Low M, Garate E, Reyes-Jara A, Murray BE, Cambiazo V, Gonzalez M (2015) Interplay between copper and zinc homeostasis through the transcriptional regulator Zur in Enterococcus faecalis. Metallomics 7:1137–1145
Leedjarv A, Ivask A, Virta M (2008) Interplay of different transporters in the mediation of divalent heavy metal resistance in Pseudomonas putida KT2440. J Bacteriol 190:2680–2689
Lewinson O, Lee AT, Rees DC (2009) A P-type ATPase importer that discriminates between essential and toxic transition metals. Proc Natl Acad Sci U S A 106:4677–4682
Lhospice S, Gomez NO, Ouerdane L, Brutesco C, Ghssein G, Hajjar C, Liratni A, Wang S, Richaud P, Bleves S et al (2017) Pseudomonas aeruginosa zinc uptake in chelating environment is primarily mediated by the metallophore pseudopaline. Sci Rep 7:17132
Lilleorg S, Reier K, Volonkin P, Remme J, Liiv A (2020) Phenotypic effects of paralogous ribosomal proteins bL31A and bL31B in E. coli. Sci Rep 10:11682
Lim CK, Hassan KA, Tetu SG, Loper JE, Paulsen IT (2012) The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5. PLoS ONE 7:e39139
Lim CK, Hassan KA, Penesyan A, Loper JE, Paulsen IT (2013) The effect of zinc limitation on the transcriptome of Pseudomonas protegens Pf-5. Environ Microbiol 15:702–715
Lopez CA, Skaar EP (2018) The impact of dietary transition metals on host-bacterial interactions. Cell Host Microbe 23:737–748
Mastropasqua MC, D’Orazio M, Cerasi M, Pacello F, Gismondi A, Canini A, Canuti L, Consalvo A, Ciavardelli D, Chirullo B et al (2017) Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by a nicotianamine-related metallophore. Mol Microbiol 106:543–561
Mazzon RR, Braz VS, da Silva Neto JF, do Valle Marques M. (2014) Analysis of the Caulobacter crescentus Zur regulon reveals novel insights in zinc acquisition by TonB-dependent outer membrane proteins. BMC Genomics 15:734
McFarlane JS, Lamb AL (2017) Biosynthesis of an opine Metallophore by Pseudomonas aeruginosa. Biochemistry 56:5967–5971
Merchant SS, Helmann JD (2012) Elemental economy: microbial strategies for optimizing growth in the face of nutrient limitation. Adv Microb Physiol 60:91–210
Mergeay M, Nies D, Schlegel HG, Gerits J, Charles P, Van Gijsegem F (1985) Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals. J Bacteriol 162:328–334
Moffett BF, Nicholson FA, Uwakwe NC, Chambers BJ, Harris JA, Hill TCJ (2003) Zinc contamination decreases the bacterial diversity of agricultural soil. FEMS Microbiol Ecol 43:13–19
Mumm K, Ainsaar K, Kasvandik S, Tenson T, Horak R (2016) Responses of Pseudomonas putida to zinc excess determined at the proteome level: pathways dependent and independent of ColRS. J Proteome Res 15:4349–4368
Olafson RW, Abel K, Sim RG (1979) Prokaryotic metallothionein: preliminary characterization of a blue-green alga heavy metal-binding protein. Biochem Biophys Res Commun 89:36–43
Osman D, Piergentili C, Chen J, Chakrabarti B, Foster AW, Lurie-Luke E, Huggins TG, Robinson NJ (2015) Generating a metal-responsive transcriptional regulator to test what confers metal sensing in cells. J Biol Chem 290:19806–19822
Panina EM, Mironov AA, Gelfand MS (2003) Comparative genomics of bacterial zinc regulons: enhanced ion transport, pathogenesis, and rearrangement of ribosomal proteins. Proc Natl Acad Sci U S A 100:9912–9917
Patzer SI, Hantke K (1998) The ZnuABC high-affinity zinc uptake system and its regulator Zur in Escherichia coli. Mol Microbiol 28:1199–1210
Paulsen IT, Saier MH Jr (1997) A novel family of ubiquitous heavy metal ion transport proteins. J Membr Biol 156:99–103
Pederick VG, Eijkelkamp BA, Begg SL, Ween MP, McAllister LJ, Paton JC, McDevitt CA (2015) ZnuA and zinc homeostasis in Pseudomonas aeruginosa. Sci Rep 5:13139
Perron K, Caille O, Rossier C, Van Delden C, Dumas JL, Kohler T (2004) CzcR-CzcS, a two-component system involved in heavy metal and carbapenem resistance in Pseudomonas aeruginosa. J Biol Chem 279:8761–8768
Price MN, Dehal PS, Arkin AP (2010) FastTree 2–approximately maximum-likelihood trees for large alignments. PLoS ONE 5:e9490
Quintana J, Novoa-Aponte L, Arguello JM (2017) Copper homeostasis networks in the bacterium Pseudomonas aeruginosa. J Biol Chem 292:15691–15704
Rodrigue A, Quentin Y, Lazdunski A, Mejean V, Foglino M (2000) Two-component systems in Pseudomonas aeruginosa: why so many? Trends Microbiol 8:498–504
Salusso A, Raimunda D (2017) Defining the roles of the cation diffusion facilitators in Fe2+/Zn2+ homeostasis and establishment of their participation in virulence in Pseudomonas aeruginosa. Front Cell Infect Microbiol 7:84
Shin J-H, Helmann JD (2016) Molecular logic of the Zur-regulated zinc deprivation response in Bacillus subtilis. Nat Commun 7:12612
Spahl DU, Berendji-Grun D, Suschek CV, Kolb-Bachofen V, Kroncke KD (2003) Regulation of zinc homeostasis by inducible NO synthase-derived NO: nuclear metallothionein translocation and intranuclear Zn2+ release. Proc Natl Acad Sci U S A 100:13952–13957
Stork M, Bos MP, Jongerius I, de Kok N, Schilders I, Weynants VE, Poolman JT, Tommassen J (2010) An outer membrane receptor of Neisseria meningitidis involved in zinc acquisition with vaccine potential. PLoS Pathog 6:e1000969
Trouillon J, Imbert L, Villard A-M, Vernet T, Attrée I, Elsen S (2021) Determination of the two-component systems regulatory network reveals core and accessory regulations across Pseudomonas aeruginosa lineages. Nucleic Acids Res 49:11476–11490
Vaccaro BJ, Lancaster WA, Thorgersen MP, Zane GM, Younkin AD, Kazakov AE, Wetmore KM, Deutschbauer A, Arkin AP, Novichkov PS et al (2016) Novel metal cation resistance systems from mutant fitness analysis of denitrifying pseudomonas stutzeri. Appl Environ Microbiol 82:6046–6056
von Rozycki T, Nies DH (2009) Cupriavidus metallidurans: evolution of a metal-resistant bacterium. Antonie Van Leeuwenhoek 96:115–139
Wang D, Chen W, Huang S, He Y, Liu X, Hu Q, Wei T, Sang H, Gan J, Chen H (2017) Structural basis of Zn(II) induced metal detoxification and antibiotic resistance by histidine kinase CzcS in Pseudomonas aeruginosa. PLoS Pathog 13:e1006533
Waterhouse A, Bertoni M, Bienert S, Studer G, Tauriello G, Gumienny R, Heer FT, de Beer TAP, Rempfer C, Bordoli L et al (2018) SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res 46:W296–W303
Wei Y, Fu D (2005) Selective metal binding to a membrane-embedded aspartate in the Escherichia coli metal transporter YiiP (FieF). J Biol Chem 280:33716–33724
Tacconelli E, Carrara E, Savoldi A, Harbarth S, Mendelson M, Monnet DL, Pulcini C, Kahlmeter G, Kluytmans J, Carmeli Y, Ouellette M, Outterson K, Patel J, Cavaleri M, Cox EM, Houchens CR, Grayson ML, Hansen P, Singh N, Theuretzbacher U, Magrini N (2018) WHO pathogens priority list working group. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect Dis 18(3):318–327. https://doi.org/10.1016/S1473-3099(17)30753-3
Wu T, Gagnon A, McGourty K, DosSantos R, Chanetsa L, Zhang B, Bello D, Kelleher SL (2021) Zinc exposure promotes commensal-to-pathogen transition in Pseudomonas aeruginosa leading to mucosal inflammation and illness in mice. Int J Mol Sci. https://doi.org/10.3390/ijms222413321
Zeitoun-Ghandour S, Leszczyszyn OI, Blindauer CA, Geier FM, Bundy JG, Sturzenbaum SR (2011) C. elegans metallothioneins: response to and defence against ROS toxicity. Mol Biosyst 7:2397–2406
Zoropogui A, Gambarelli S, Coves J (2008) CzcE from Cupriavidus metallidurans CH34 is a copper-binding protein. Biochem Biophys Res Commun 365:735–739
Acknowledgements
The work in K.P. laboratory is supported by the Swiss National Science Foundation (grant 31003A_179336). The work in D.G. laboratory is supported by a Swiss National Science Foundation Ambizione grant (PZ00P3_180142). The authors thank X. Ravinet (Scientify, University of Geneva, Switzerland) for preparing Figures 1 and 3.
Funding
Open access funding provided by University of Geneva.
Author information
Authors and Affiliations
Contributions
V.D., D.G. and K.P. devised the manuscript. V.D. and K.P. designed figures 1 to 4. D.G. prepared tables 1 and 2. V.D., D.G. and K.P. wrote the manuscript.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Ducret, V., Gonzalez, D. & Perron, K. Zinc homeostasis in Pseudomonas. Biometals 36, 729–744 (2023). https://doi.org/10.1007/s10534-022-00475-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10534-022-00475-5