Abstract
Rutin is a flavonoid glycoside found in many plants. It is used as medication and an ingredient of numerous multivitamin preparations and herbal remedies due to its biomedical activity. The present paper deals with transformation processes of rutin during its extraction by methanol, ethanol, and their aqueous mixtures from Aronia melanocarpa (M.) E. and Sambucus nigra L. fruits, Hypericum perforatum L. herb, and Tussilago farfara L. leaves, known as ones of the most riches in rutin plant materials. It has been established that the transformation degree of rutin during its extraction depends not only on extraction time and extractant type but also, to a high degree, on the plant matrix type. Differences in the degree of rutin transformation are influenced by the matrix components in the examined plants. The reported results are particularly important for researchers investigating plant metabolism and looking for new plant components.
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Introduction
More and more attention has been paid recently to the role and application of secondary plant metabolites which, due to their biochemical properties, are often applied by physicians, pharmacists, dieticians, etc. (Bourgaud et al. 2001; Crozier et al. 2006; Kennedy and Wightman 2011; Pandey and Rizvi 2009). Rutin, being a secondary plant metabolite, is one of the highly valued compounds. Its antifungal, antibacterial, antiinflammatory and anticancer properties and the ability of radical scavenging are most frequently discussed (Biesaga 2011; Guardia et al. 2001; Mohd Zainol et al. 2009; Navarro-Núñez et al. 2008; Wang et al. 2011; Yang et al. 2008; You et al. 2010). These properties require better knowledge of rutin itself, its transformations, and its occurrence and metabolism in plants. An inquiry into the analytical procedures involving rutin is an important step in this cognition process. The analysis of plant constituents requires the application of sample preparation methods allowing for full isolation of the analyzed substances from the plant matrix (Dawidowicz and Wianowska 2009). The most procedures of rutin determination in plants involve their high-temperature extraction prior to HPLC analysis and the application of methanol and methanol-water mixtures as extractants (Andrade et al. 1997; Dawidowicz et al. 2003; Suárez-Quiroz et al. 2014; Uppugundla et al. 2009; Wianowska et al. 2015).
In our previous studies (Dawidowicz et al. 2016), we found that as many as 23 derivative compounds and reaction products with methanol were formed from rutin during its extraction by methanol or methanol/water solution. These compounds can be mistakenly treated as new compounds not found before in the examined plant or can be a cause of erroneous quantitative estimations of plant composition. We concluded that more detailed experiments with rutin extraction from flowers of Sambucus nigra L. were needed to determine the possible influence of the plant matrix type on rutin transformation and degradation processes. To see if this is indeed the case, we examined in this study the transformation process of rutin during its extraction from Aronia melanocarpa (M.) E. and S. nigra L. fruits, Hypericum perforatum L. herb, and Tussilago farfara L. leaves, rutin-rich plant materials. As the rutin concentration level in the examined plant materials is different, all extractions were performed with a similar rutin amount in the extraction systems to maintain a similar quantitative ratio between rutin and extractant. Consequently, the amounts of the selected plant materials in the extraction processes had to be different. Methanol, ethanol, and their mixtures with water were applied as extractants in all the experiments.
Materials and Methods
Materials and Reagents
The A. melanocarpa fruits (M.) E., S. nigra L. fruits, H. perforatum L. herb, and T. farfara L. leaves, cultivated in the eastern part of Poland, were air-dried at +35 °C and stored at +4 °C. Acetonitrile (HPLC), methanol, and ethanol were purchased from the Polish Chemical Plant POCh (Gliwice, Poland) and formic acid from Sigma-Aldrich (Seelze, Germany). Water was purified on the Milli-Q system from Millipore (Millipore, Bedford, MA, USA).
Investigations of Rutin Transformation Process During Rutin Extraction from Plant Materials
Investigations of the rutin transformation process were performed by heating plant material suspensions in methanol or ethanol or alcohol/water (75/25 v/v) mixtures. The glass equipment consisted of a boiling flask (100 mL) and a small condenser. The heated plant suspensions were composed of 50 mL of a given extractant and an amount of plant material containing about 10 mg of rutin. Rough rutin concentration in the examined plants was established prior to the experiments following the procedure described below. Individual plant suspensions were heated for 1 or 2 or 3 h. The extracts were centrifuged, and the obtained supernatants were subjected to liquid chromatography-mass spectrometry-diode array detector (LC-MS-PDA) analysis.
Estimation of Rutin Concentration in Examined Plant Materials
The exactly weighed portions (0.3 g) of grounded plant materials were ultrasonicated with 50 mL of methanol/water (75/25 v/v) mixture for 1 h at 40 °C. Subsequently, the supernatants isolated by centrifuging were subjected to LC-MS-PDA analysis.
HPLC Measurements
The chromatographic measurements were performed using LC/MS from Finnigan (LCQ Advantage Max) equipped with the ion-trap mass spectrometric system (Thermo Electron Corporation, San Jose, CA) and diode array detector from Finnigan (Surveyor PDA Plus Detector). The Gemini C18 column (4.6 × 100 mm, 3 μm) (Phenomenex, USA) was employed for chromatographic separation, which was performed using gradient elution and flow rate of 0.4 mL/min. The mobile phase A was 25 mM formic acid in water; the mobile phase B was 25 mM formic acid in acetonitrile. The gradient program started at 5 % B increasing to 35 % for 60 min, next 35 % B to 95 % B for 12 min, and ended with isocratic elution followed (95 % B) for 3 min. In the course of each run, PDA spectra in the range 190–600 nm and MS spectra in the range of 100–2000 m/z were collected continuously. In all rutin solutions and S. nigra L. fruits, the selected ion monitoring (SIM) function was used to better visualize the chromatographic separation and to remove the signal connected with rutin, plant matrix, and buffer components. The time periods and monitored ions were as follows:
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0–15.5 min (197 m/z), 15.5–16 min (211 m/z), 16–17 min (179 m/z), 17–17.5 min (225 m/z), 17.5–18 min (305 m/z), 18–19 min (335 m/z), 19–20 min (193 m/z), 20–21 min (319 m/z),
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21–23 min (317 m/z), 23–25 min (207 m/z), 25–27 min (331 m/z), 27–29 min (257 m/z),
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29–31 min (345 m/z), 31–34 min (271 m/z), 34–34.5 min (349 m/z), 34.5–35 min (333 m/z),
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35–40 min (363 m/z), 40–42 min (463 m/z), 42–45 min (623 m/z), 45–46 min (347 m/z),
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46–47 min (447 m/z), 47–48 min (637 m/z), 48–49 min (477 m/z), 49–51 min (491 m/z),
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51–56 min (461 m/z), 56–59 min (475 m/z), 59–63 min (301 m/z), 63–65 min (273 m/z),
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65–67 min (361 m/z), 67–71 min (315 m/z), 71–75 min (329 m/z).
The column effluent was ionized by electrospray ionization (ESI). The ESI was operated in negative polarity mode under the following specific conditions: spray voltage—4.5 kV, sheath gas—40 arbitrary units, auxiliary gas—10 arbitrary units, sweep gas—10 arbitrary units, and capillary temperature—320 °C. Nitrogen (>99.98 %) was employed as sheath, auxiliary, and sweep gas. All eluate was subjected to ionization. The collision energy was chosen individually for the examined compounds.
To identify rutin derivatives (the products of its hydrolysis, transformation, and oxidation), the function of secondary (MS2) ion fragmentation was applied. The collision energy for each examined compound was chosen individually. For confirmation, high-resolution mass spectrometry (HRMS) analysis was additionally performed.
The HRMS analysis was carried out on the HPLC system coupled to a linear trap quadrupole-Orbitrap mass spectrometer (LTQ-Orbitrap Velos from Thermo Fisher Scientific, San Jose, CA) equipped with an ESI source. The ESI was operated in negative polarity modes under the following specific conditions: spray voltage—3.5 kV, sheath gas—40 arbitrary units, auxiliary gas—10 arbitrary units, sweep gas—10 arbitrary units, and capillary temperature—320 °C. Nitrogen (>99.98 %) was employed as sheath, auxiliary, and sweep gas. The scan cycle used a full-scan event at the resolution of 60,000. All eluate was subjected to ionization. The collision energy was chosen individually for the examined compounds.
Due to the lack of standards of rutin derivatives, their amounts were estimated by relating their chromatographic responses to the calibration curve for rutin and its aglycone, quercetin.
The calibration curve for rutin was used for estimating the amounts of the following glycoside rutin derivatives:
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2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one (isoquercitrin);
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2-(3,4-Dihydroxyphenyl)-7-hydroxy-5-methoxy-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one (methyl-isoquercitrin);
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2-(3,4-Dihydroxyphenyl)-5-ethoxy-7-hydroxy-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one (ethyl-isoquercitrin);
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2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxychromen-4-one (quercitrin);
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2-(3,4-Dihydroxyphenyl)-7-hydroxy-5-methoxy-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxychromen-4-one (methyl-quercitrin);
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2-(3,4-Dihydroxyphenyl)-5-ethoxy-7-hydroxy-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxychromen-4-one (ethyl-quercitrin);
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2-(3,4-Dihydroxyphenyl)-7-hydroxy-5-methoxy-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxymethyl]oxan-2-yl]oxychromen-4-one (methyl-rutin);
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2-(3,4-Dihydroxyphenyl)-5-ethoxy-7-hydroxy-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxymethyl]oxan-2-yl]oxychromen-4-one (ethyl-rutin);
The calibration curve for quercetin was applied for estimating the amounts of the following aglicon rutin derivatives:
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2-[(3,4-Dihydroxyphenyl)carbonyl]-2,4,6-trihydroxy-1-benzofuran-3(2H)-one (DTB);
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2-[(3,4-Dihydroxyphenyl)carbonyl]-4,6-dihydroxy-2-methoxy-1-benzofuran-3(2H)-one (methyl-DTB);
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2-[(3,4-Dihydroxyphenyl)carbonyl]-2-ethoxy-4,6-dihydroxy-1-benzofuran-3(2H)-one (ethyl-DTB);
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Oxo(2,4,6-trihydroxyphenyl)acetic acid (OTA);
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Methyl oxo(2,4,6-trihydroxyphenyl)acetate (methyl-OTA);
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Ethyl oxo(2,4,6-trihydroxyphenyl)acetate (ethyl-OTA);
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2-[Carboxy(3,4-dihydroxyphenyl)methoxy]-4,6-dihydroxybenzoic acid (CDA);
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2-[1-(3,4-Dihydroxyphenyl)-2-methoxy-2-oxoethoxy]-4,6-dihydroxybenzoic acid (methyl-CDA);
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2-[1-(3,4-Dihydroxyphenyl)-2-ethoxy-2-oxoethoxy]-4,6-dihydroxybenzoic acid (ethyl-CDA);
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2-{[(3,4-Dihydroxyphenyl)carbonyl]oxy}-4,6-dihydroxybenzoic acid (DDA);
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Methyl 2-{[(3,4-dihydroxyphenyl)carbonyl]oxy}-4,6-dihydroxybenzoate (methyl-DDA);
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Ethyl 2-{[(3,4-dihydroxyphenyl)carbonyl]oxy}-4,6-dihydroxybenzoate (ethyl-DDA);
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4,6-Dihydroxy-1-benzofuran-2,3-dione (DBD);
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6-Hydroxy-4-methoxy-1-benzofuran-2,3-dione (methyl-DBD);
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4-Ethoxy-6-hydroxy-1-benzofuran-2,3-dione (ethyl-DBD);
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7-(3,4-Dihydroxyphenyl)bicyclo[4.2.0]octa-1,3,5,7-tetraene-2,4,8-triol (DBOT);
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7-(3,4-Dihydroxyphenyl)-8-methoxybicyclo[4.2.0]octa-1,3,5,7-tetraene-2,4-diol (methyl-DBOT);
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3,5-Dihydroxy-2-[methoxy(oxo)acetyl]phenyl 3,4-dihydroxybenzoate (DPD);
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5-Hydroxy-3-methoxy-2-[methoxy(oxo)acetyl]phenyl 3,4-dihydroxybenzoate (methyl-DPD);
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2-(3,4-Dihydroxyphenyl)-3,5,7-trihydroxychromen-4-one (quercetin);
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2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-3-methoxychromen-4-one (methyl-quercetin);
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2-(3,4-Dihydroxyphenyl)-3-ethoxy-5,7-dihydroxychromen-4-one (ethyl-quercetin);
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2-(3,4-Dihydroxyphenyl)-1-benzofuran-3,4,6-triol (DBT).
Statistical Analysis
All the results are presented as the mean of three independent measurements (n = 3). Differences in the concentration of the formed rutin derivatives were compared using the analysis of variance (ANOVA, p = 0.05). Differences in the studied group were considered significant for p value lower than 0.05 and F value higher than 4.26. The ANOVA revealed statistically significant differences only for a few tested groups.
Results and Discussion
Figure 1 presents the exemplary chromatograms of methanolic (Fig. 1a), methanol/water (Fig. 1b), ethanolic (Fig. 1c), and ethanol/water (Fig. 1d) supernatants after 3-h extraction of H. perforatum L. herb under reflux. Figure 1a, b illustrates the presence of almost all rutin derivatives (formed during the heating of methanolic or methanol/water rutin solution; Dawidowicz et al. 2016) in the methanolic or methanol/water extract of the herb. Yet, it is uncertain which of them are native components of H. perforatum L. The chromatographic analysis (Fig. 1c) shows that 17 rutin transformation products and their ethyl derivatives are present in H. perforatum L. ethanolic extract. The absence of methyl derivatives of rutin and its transformation products in ethanolic extracts and, vice versa, the absence of ethyl derivatives of rutin and its transformation products in methanolic extracts of H. perforatum L. prove that alcoholic rutin derivatives are non-native H. perforatum L. components formed during the rutin extraction process from the plant. The same conclusion is valid for the fruits of A. melanocarpa (M.) E. and S. nigra L. and the leaves of T. farfara L. The exemplary chromatograms of their methanolic, methanol/water, ethanolic, and ethanol/water extracts are shown in Figs. S1a–f and Fig. S2a–f, respectively (Supplementary Material). The structures of all rutin derivatives found in the examined extracts are shown in Fig. 2. All of them were identified and confirmed on the basis of the retention data of their standards, the PDA, MS2, HRMS, and literature data (Dubber et al. 2005; Wang et al. 2011; Zenkevich et al. 2007; Zvezdanović et al. 2012). The MS2 and HRMS data for the identified compounds are collected in Tables 1 and 2, respectively. MS1 and MS2 spectra for one exemplary rutin derivative, OTA, are presented in Fig. S3 (Supplementary Material).
While the alcoholic rutin derivatives are evidently non-native components of the examined plants, it is more difficult to determine unequivocally to the origin of the non-alcoholic rutin derivatives. There is no doubt about quercetin, isoquercitrin, and quercitrin which are truly native plant components involved in the rutin metabolic pathway, but the origin of DTB, OTA, CDA, DDA, DPD, DBT, DBD, and DBOT is less certain. As shown in Dawidowicz et al. (2016), they are formed during the heating of methanolic and methanol/water rutin solution, and they are also known as rutin and/or quercetin degradation products forming during their high-temperature treatment, UV irradiation, or exposure to other aggressive factors (Dubber et al. 2005; Rohn et al. 2007; Wang et al. 2011; Zenkevich et al. 2007; Zvezdanović et al. 2012). The presented data show that some of them are formed during the heating of ethanolic and ethanol/water rutin solution.
As methanol-water mixtures containing 70–80 % of the alcohol are the most frequently applied extrahents for the isolation of flavonoids from plant materials (Andrade et al. 1997; Dawidowicz et al. 2003; Suárez-Quiroz et al. 2014; Uppugundla et al. 2009; Wianowska et al. 2015), the examination of the influence of extraction time on the amounts of rutin derivatives formed during rutin extraction from different matrix type was performed with methanol/water mixture 75/25. The results of these investigations are presented in Fig. 3, the structure of which results from the fact that a rutin transformation and degradation products in methanol/water mixture exist in two forms (Dawidowicz et al. 2016): as non-methyl and methyl derivatives. Therefore, the left part of the figure (Fig. 3a1–d1) concerns non-methyl derivatives whereas the right part (Fig. 3a2–d2) contains the results corresponding to methyl rutin derivatives. The presented relationships demonstrate two transformation trends in the course of time:
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The growth of rutin derivatives
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The initial growth followed by a decrease of rutin derivatives (see compounds nr 2, 6, 8, 1’, 9’, 10’, and 12’ in Fig. 3).
The rates of the observed concentration changes for individual compounds and plant matrices are different. The growing concentration of rutin derivatives during extraction time increase can be easily explained for non-native plant compounds by the increase of degradation degree of rutin and for native plant components (quercetin, quercitrin, isoquercitrin) by the increase of extraction efficiency. The observed initial increase of the amount of some rutin derivatives and the subsequent decrease can be treated as experimental error (see error bars). However, this behavior can also be the result of a simultaneous compound formation and degradation processes. The latter explanation is more probable since the trend is observed for the same substances irrespective of the applied plant matrix (Fig. 3). The formation of DPD and its degradation to OTA or the formation of methyl-quercetin and its degradation to methyl-CDA can be examples of such simultaneous processes.
In order, to determine the influence of the plant matrix type on the rutin transformation and degradation process, all extraction systems used in the experiments contained a similar amount of rutin but different amounts of individual plant materials. This rule ensured a similar initial quantitative ratio between rutin and extractant for all examined plants and made the comparison of the matrix type influence much easier. The analysis of the amounts of individual rutin derivatives formed during rutin extraction from the examined plant materials (see Fig. 3) proves that the plant matrix does not change of the shape of the observed dependences but influences significantly the intensity of the rutin transformation process. The greatest changes for non-methyl rutin derivatives are observed in the case of T. farfara L. leaves and for methyl rutin derivatives in the case of S. nigra L. fruits (see compounds 3, 4, and 5 in Fig. 3d1 and compounds 7’ and 9’ in Fig. 3b2, respectively).
The latter conclusion suggests that some substances present in the individual plant matrices catalyze or inhibit the rutin transformation process during its extraction.
The ethanolic extract of plant materials containing rutin appears in many pharmaceutical formulations. For this reason, it was decided to compare the rutin transformation process during rutin extraction from the examined plant materials using methanol, ethanol, and their water mixtures containing 75 % of alcohol. The results of the experiments for a 3-h extraction process are shown in Fig. 4 and lead to the following conclusions:
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Greater amounts of non-alcohol and alcohol rutin derivatives are formed in pure alcohols than in alcohol/water mixture.
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Greater amounts of non-alcohol and alcohol rutin derivatives are formed in methanol and methanol/water mixture than in ethanol and ethanol/water mixture. It is probably connected with greater acidity and reactivity of methanol in relation to ethanol.
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The impact of plant material components on the rutin transformation process is observed in the both alcohols, yet more evidently in methanol and methanol/water mixture.
Conclusions
As results from Dawidowicz et al. (2016), the heating of methanolic or methanol/water rutin solution under reflux as well as the extraction process of rutin from S. nigra L. flower by methanol and methanol/water mixture causes rutin transformations to its methyl and non-methyl derivatives. The present paper shows that similar transformation processes occur during rutin extraction from other plant materials when methanol, ethanol, and their water mixtures are used as extractants. As results from the obtained data, the degree of rutin transformation in the extraction process depends not only on extraction time and extractant type but also strongly on the plant matrix type. The different degree of rutin transformation is probably caused by the plant matrix components in the examined plants. As also suggested in Dawidowicz et al. (2016), two possible processes of rutin transformation and degradation during its extraction should be considered:
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The formation of alcoholic rutin derivatives in the reaction of non-alcoholic rutin derivatives with alcohol. In consequence, the alcoholic rutin derivatives are the final products of the rutin transformation and degradation process;
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The formation of non-alcoholic rutin derivatives from alcoholic rutin derivatives.
As rutin transformation can be a two-step process (see above points considering two-step rutin degradation), the determination of a positive or a negative catalytic role played in this process by different plant matrix components is difficult.
The transformation products of rutin can be mistakenly treated as new components, not naturally present in the examined plant. Alternatively, they can be responsible for the natural plant composition when some or all of them naturally exist in the examined plant, and the transformation process only increases their amounts. This is why our results are especially useful for researchers investigating plant metabolism and looking for new plant components.
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Fig. S1
Exemplary chromatograms of methanolic supernatants of Aronia melanocarpa (M.) E. fruits (a), Sambucus nigra L. fruits (b), Tussilago farfara L. leaves (c) and methanol/water 75/25 v/v supernatants of these plant materials (d, e, f, respectively) after 3 h extraction under reflux. Peak numbers correspond to compounds numbers reported in Fig. 2 (GIF 488 kb)
Fig. S2
Exemplary chromatograms of ethanolic supernatants of Aronia melanocarpa (M.) E. fruits (a), Sambucus nigra L. fruits (b), Tussilago farfara L. leaves (c) and ethanol/water 75/25 v/v supernatants of these plant materials (d, e, f, respectively) after 3 h extraction under reflux. Peak numbers correspond to compounds numbers reported in Fig. 2 (GIF 527 kb)
Fig. S3
Exemplary MS1 and MS2 spectrum for OTA compound (GIF 173 kb)
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Dawidowicz, A.L., Bernacik, K. & Typek, R. Does the Plant Matrix Type Have Impact on Rutin Transformation During Its Extraction?. Food Anal. Methods 9, 2042–2051 (2016). https://doi.org/10.1007/s12161-015-0388-z
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DOI: https://doi.org/10.1007/s12161-015-0388-z