Abstract
Bone constantly remodels between resorption by osteoclasts and formation by osteoblasts; therefore the functions of osteoblasts are pivotal for maintaining homeostasis of bone mass. Transient receptor potential vanilloid 4 (TRPV4), a type of mechanosensitive channel, has been reported to be a key regulator in bone remodeling. However, the relationship between TRPV4 and osteoblast function remains largely elusive. Only little is known about the spatial distribution change of TRPV4 during osteoblastic differentiation and related signal events. Based on three-dimensional super-resolution microscopy, our results clearly showed a different distribution of TRPV4 in undifferentiated and differentiated osteoblasts, which reflected the plasma membrane translocation of TRPV4 along with prolonged differentiation. GSK1016790A (GSK101), the most potent agonist of TRPV4, triggered rapid calcium entry and calmodulin-dependent protein kinase II (CaMKII) phosphorylation via TRPV4 activation in a differentiation-dependent manner, indicating that the abundance of TRPV4 at the cell surface resulting from differentiation may be related to the modulation of Ca2+ response and CaMKII activity. These data provide compelling evidences for the plasma membrane translocation of TRPV4 during osteoblastic differentiation as well as demonstrate the regulation of downstream Ca2+/CaMKII signaling.
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Introduction
Bone is a dynamic tissue that undergoes continuous remodeling between formation of new bone matrix by osteoblasts and resorption of preexisting bone matrix by osteoclasts (Crockett et al.2011). A proper balance between osteoblasts and osteoclasts is vital for maintaining bone construction and function (Raggatt and Partridge 2010). Attenuation of osteoblast formation may lead to bone loss, osteoporosis, and eventually debilitating fractures (Zaidi 2007). Osteoblasts originate from mesenchymal progenitors that, with the appropriate stimulation, differentiate into preosteoblasts and then to mature, functional osteoblasts (Kassem et al.2008). Mature osteoblasts express many biochemical markers and transcription factors (Gundberg 2000; Marie 2008). By undergoing extracellular matrix maturation and mineralization, osteoblasts form bone-like mineralized nodules in vivo and in vitro (Blair et al.2017). An increase in proliferation results in a greater number of bone-forming cells, whereas increased differentiation enhances the capability of osteoblasts to synthesize and secrete osteoid matrix. Therefore, osteoblastic differentiation plays essential roles in bone formation and mineralization, which can be regulated by various signals including hormones, cytokines, and external factors such as mechanical loading (Katagiri and Takahashi 2002; Long 2011).
Mechanotransduction has been recognized as one of the major factors regulating bone remodeling (Ehrlich and Lanyon 2002); however, it is still unclear how bone cells perceive this type of stimulation. It has been established that mechanical stimulus activates some mechanosensitive cation channels particularly the transient receptor potential (TRP) channels on the plasma membrane (Christensen and Corey 2007; Maycas et al.2017). In the TRP superfamily, Transient receptor potential vanilloid 4 (TRPV4) is predominantly expressed in various types of bone cells including osteoblasts and serves as a potential “mechanosensor” (Abed et al.2009; Guilak et al.2010). As a type of polymodal channel, TRPV4 is capable of sensing multiple stimuli from the environment, such as heat, hypotonicity, acidity, and endogenous lipids downstream of arachidonic acid metabolism besides mechanical force (Garcia-Elias et al.2014; White et al.2016). Opening of TRPV4 channels will lead to increase in the intracellular Ca2+ concentration owing to Ca2+ influx, thereby acting as a central player in calcium signaling (Nilius et al.2004). The role of TRPV4 in bone cells has been studied primarily using osteoclasts and chondrocytes (Masuyama et al.2008; O’Conor et al.2014). For instance, TRPV4-mediated calcium influx was found to regulate the terminal differentiation of osteoclasts (Masuyama et al.2008), and the activation of TRPV4 was responsible for promoting chondrogenesis through a Ca2+/calmodulin pathway (O’Conor et al.2014). Furthermore, accumulating evidences indicated that TRPV4 mutations were linked to a variety of skeletal dysplasias (Kang et al.2012; Leddy et al.2014). These studies potently supported the fundamental importance of TRPV4 in normal bone remodeling. However, previous work showed conflicting data regarding the participation of TRPV4 in osteoblast functions. Some studies proposed that TRPV4 repressed alternative mesenchymal differentiation pathways such as adipocyte and chondrocyte differentiation, while it promoted osteoblast proliferation, differentiation and mineralization activity, and blocked osteoblast apoptosis (Kang et al.2012; O’Conor et al.2013). In contrast, there are reports suggesting no influence (Masuyama et al.2008) or even an inhibitory effect (van der Eerden et al.2013) of TRPV4 on osteoblast functions. Notably, a recent work revealed that osteoblastic differentiation enhanced the expression level of TRPV4, which is required for calcium oscillation induced by fluid flow (Suzuki et al.2013).
Therefore, in the present study, we aimed to determine the distribution change of TRPV4 channels during osteoblast differentiation and then investigated how it influenced the cellular responses and signal transduction. Based on three-dimensional (3D) super-resolution microscopy, our results directly and visually revealed the plasma membrane translocation of TRPV4 in differentiated osteoblasts. Moreover, we found that the recruitment of TRPV4 to the membrane resulted from differentiation regulated downstream Ca2+/calmodulin-dependent protein kinase II (CaMKII) signaling.
Results
Osteoblastic differentiation enhanced the membrane protein expression of TRPV4
Firstly, we detected the expressions of TRPV4 in the isolated rat calvarial osteoblasts at different stages of differentiation (cells grown to differentiate in osteogenic medium for 0, 1, 3, 5, 7 days). As shown in Fig. 1A, the mRNA expression of TRPV4 was relatively low in undifferentiated osteoblasts (day 0). In contrast, the levels of TRPV4 mRNA were increased in parallel to the increase in differentiation, which corroborated previous findings (Suzuki et al.2013), which somehow declined on day 7. Meanwhile, the mRNA levels of pacsin3, a protein that has been reported to modulate the membrane localization of the TRPV4 (Cuajungco et al.2006), were also potently elevated during differentiation (Fig. 1B). We further examined the membrane and cytoplasmic protein expression of TRPV4 by Western blotting. Results showed that the membrane protein level of TRPV4 in osteoblasts was robustly enhanced along with prolonged differentiation, achieving maximum on day 3 and remaining constant thereafter on day 5 and day 7 (Fig. 1C and D). Accordingly, TRPV4 protein expression in the cytoplasm was gradually reduced upon differentiation (Fig. 1C and D). It exhibited a double band of TRPV4, which was considered to be the glycosylated form of TRPV4 from previous work (Xu et al.2006). Besides, the osteoblastic differentiation on day 5 has been demonstrated by testing the expression level of marker genes of osteoblast differentiation (supplementary Fig. S1).
3D-STORM imaging demonstrated the plasma membrane translocation of TRPV4 channels during osteoblastic differentiation
Based on 3D-STORM, we clearly visualized the nanoscale spatial distribution of TRPV4 channels in osteoblasts. Representative STORM images from undifferentiated and differentiated groups are shown in Fig. 2 and supplementary Fig. S2, which potently improved the lateral and axial resolution compared with conventional images. In the undifferentiated group (day 0), we only observed a single-layer distribution of TRPV4 from the z direction (Fig. 2Ad). Gaussian fit of the count data acquired by STORM imaging also depicted a single-peak profile that quantitatively confirmed this single-layer distribution (Fig. 2Ae and Af). However, TRPV4 in differentiated cells (day 5) showed a distinct arrangement of two vertically separated layers, which was also verified by the two-peak Gaussian fit curves (Fig. 2B). The two layers can be spaced apart by more than 400 nm (Fig. 2Be and Bf). Correspondingly the structure of the cell body from z direction, and the upper and bottom layers represent the plasma membrane towards the medium and the glass slide, respectively, and the interior region between the two layers stands for intracellular space. It also showed that the intensity of the upper layer was usually stronger than the bottom one, implying the membrane proteins tend to distribute towards the medium environment (Fig. 2Be and Bf). Due to the heterogeneity of primary rat calvarial osteoblast cultures, we evaluated the proportion of cells that showed the difference in subcellular distribution of TRPV4. The percentage of cells with two-layer distribution were 0% and (76.2 ± 8.2) % in day 0 and day 5 groups, respectively (Fig. 2C). Supplementary Fig. S2 provided additional examples of the contrasting distribution changes of TRPV4 during osteoblastic differentiation. Besides, we carried out negative control experiments to establish the specificity of the TRPV4 antibody in supplementary Fig. S3. These results evidently indicated that TRPV4 localization differs in the two differentiation stages of osteoblasts. TRPV4 mainly distributed in intracellular compartment in undifferentiated conditions, but it was predominantly located at the plasma membrane in mature osteoblasts. Overall, our results based on these two methods provided convincing evidences for the plasma membrane translocation of TRPV4 channels during osteoblastic differentiation.
Effects of osteoblast differentiation on TRPV4-mediated [Ca2+]i dynamics
Subsequently, the intracellular Ca2+ responses induced by specific TRPV4 agonist GSK1016790A (GSK101) were measured. Results showed that GSK101 dose-dependently triggered [Ca2+]i increase in osteoblasts both on day 0 (Fig. 3A) and day 5 (Fig. 3B). It is obvious that the Ca2+ response amplitude of GSK101-evoked [Ca2+]i change in differentiated cells (day 5) were stronger than that of undifferentiated cells (day 0) (Fig. 3C), implying a strengthened Ca2+ response resulted from differentiation. To investigate the mechanisms underlying the [Ca2+]i increase, we chose a concentration of 2 nmol/L GSK101 for the following experiments. For cells on day 5, GSK-induced [Ca2+]i increase was completely abolished in calcium-free buffer (Fig. 3D and E), suggesting that the [Ca2+]i increase was mostly attributed to extracellular Ca2+ entry. On the other hand, the [Ca2+]i increase was almost eliminated after pretreatment with GSK205 (TRPV4 specific antagonist, 0.5 and 5 μmol/L) and ruthenium red (RR, non-specific TRPV blocker, 0.5 and 5 μmol/L) for 15 min (Fig. 3D and E), revealing that the Ca2+ entry elicited by GSK101 relied on the activation of TRPV4. Remarkably, the [Ca2+]i increase in response to GSK101 caused either an oscillatory or a sustained plateau pattern (supplementary Fig. S4); the former was known to play more important roles in regulating osteoblast gene expression and function (Meng et al.2018; Suzuki et al.2013). As summarized in Fig. 3 and supplementary Fig. S4, with prolonged cell differentiation, the Ca2+ response rate (Fig. 3F), response amplitude (Fig. 3G) as well as Ca2+ oscillation rate (supplementary Fig. S4) induced by GSK101 (2 nmol/L) were significantly increased, while the delay time of Ca2+ response was short in day 3 and day 5 groups compared with day 0 group (Fig. 3H). Altogether, these data demonstrated that TRPV4-mediated Ca2+ responses were associated with cell differentiation. The membrane translocation of TRPV4 during differentiation hereby provided an explanation for the strengthened Ca2+ response in differentiated cells.
Activation of TRPV4 led to rapid phosphorylation of CaMKII in differentiated osteoblasts
In osteoblasts, Ca2+ signaling is mainly mediated by the Ca2+ binding protein calmodulin (CaM) and CaM-dependent protein kinases (CaMKs), as well as NFAT (Winslow et al.2006; Zayzafoon 2006). Therefore, we tested the activities of CaMKII to further explore the signal downstream of TRPV4-mediated Ca2+ influx. Firstly, we examined the expressions of CaMKII and phosphorylated CaMKII (p-CaMKII) simultaneously at different stages of differentiation. The protein levels of CaMKII and p-CaMKII were almost invariant on day 0–7, with only a slight increase of p-CaMKII in cells on day 5 (Fig. 4A and B). Notably, application with GSK101 (2 nmol/L) for 10 min evoked robust phosphorylation of CaMKII on day 5 in contrast to day 0, whereas it recovered to the base value when treated for 1 h (Fig. 4C and D). In response to GSK101, the phosphorylation of CaMKII was immediately detected after the calcium entry mediated by the activation of TRPV4. Hence, the above results suggest a crucial role of TRPV4 activation for the regulation of CaMKII.
Discussion
The differentiation of osteoblasts is an essential process for bone construction and function (Blair et al.2017; Kassem et al.2008). In this work, it was found that the membrane protein level of TRPV4 in osteoblasts isolated from the calvarias of new born rats was gradually increased, while cytoplasmic protein decreased along with prolonged osteoblastic differentiation (Fig. 1). Then, 3D-STORM results clearly showed a different distribution pattern of TRPV4 in differentiated osteoblasts, which reflected the plasma membrane translocation of TRPV4 (Fig. 2 and supplementary Fig. S2). Further, stimulation with specific TRPV4 agonist GSK101 dose-dependently induced [Ca2+]i increases due to TRPV4-mediated calcium entry (Fig. 3A–E). The rate and amplitude of GSK101-evoked Ca2+ response were dependent on the differentiation of osteoblasts (Fig. 3F–H). These data indicated that the strengthened [Ca2+]i increases in response to GSK101 should be attributed to the activation of TRPV4 that translocated to the plasma membrane during differentiation. Subsequently, GSK101 induced rapid phosphorylation of CaMKII in differentiated cells (Fig. 4), suggesting that the activation of CaMKII was downstream TRPV4-mediated calcium entry. All these data clearly suggested a pivotal role for TRPV4 membrane translocation during osteogenic differentiation in the modulation of intracellular Ca2+/CaMKII signaling.
Nowadays, mechanosensitive TRP channels have been recognized to be a key sensor and regulator in the development and remodeling of the skeleton (Lieben and Carmeliet 2012); therefore it is important to define their roles in the function of bone cells including osteoblasts. Cellular activity of TRP channels is subjected to a complex modulation encompassing from posttranslational modification to regulation of their abundance at the cell surface (Darby et al.2016). Currently, the transport and mobilization of these channels to the cell membrane are being an area of intense investigation (Ferrandiz-Huertas et al.2014). It has been demonstrated however that TRPV2 channels were stored in intracellular pools such as endoplasmic reticulum in non-stimulated conditions and then get translocated to the plasma membrane and function as a cation channel upon stimulation of the cells by ligands, mechanical stress, or insulin-like growth factor (IGF) (Flockerzi and Nilius 2014). It has also been suggested that TRPM2 translocated to the plasma membrane during microglial activation in response to external stimuli (Echeverry et al.2016). Similarly, we found a translocation process of TRPV4 channels during osteoblastic differentiation based on traditional method—Western blotting (Fig. 1) and powerful 3D super-resolution microscopy (Fig. 2 and supplementary Fig. S2). Due to diffraction limit, traditional fluorescence microscopy can at most provide information at the subcellular level rather than directly monitoring the distribution features of TRPV4 at the single-molecule level. The rise of super-resolution microscopy over the past decade significantly surpass the diffraction limit and achieved a nanoscale lateral/axial resolution (Huang et al.2008), which makes it highly desirable for investigation of spatial organization and distribution of membrane proteins with nanometer precision (AbuZineh et al.2018; Roh et al.2015; Yan et al.2018). Herein, for the first time, we used 3D-STORM super-resolution microscopy to visually and directly explore the localization and distribution pattern of TRPV4 in different stages of osteoblasts, which demonstrated that TRPV4 translocated from intracellular compartments to the plasma membrane during differentiation (Fig. 2 and supplementary Fig. S2).
Furthermore, we found that this membrane translocation of TRPV4 contributed to the modulation of Ca2+ response in osteoblasts. Acting as a type of ion channel, the opening of TRPV4 will initially result in the elevation of intracellular Ca2+ concentrations ([Ca2+]i), which are crucial for bone homeostasis (Meng et al.2018; Zayzafoon 2006). Activating TRPV4 by its agonist GSK101 triggered significant [Ca2+]i increases as expected (Fig. 3 and supplementary Fig. S4). Interestingly, the GSK101-elicited Ca2+ response in differentiated cells was more rapid and stronger than that of undifferentiated cells (Fig. 3 and supplementary Fig. S4). Given that TRPV4 channels serve as mechanosensor only when they are located on the plasma membrane, the recruitment of TRPV4 to the cell surface may be a crucial step in the physiological functioning of the channel and tightly controlled to ensure the proper regulation of intracellular ion homeostasis and signal transduction. Therefore, the trafficking of TRPV4 to plasma membrane provides a persuasive explanation for the strengthened intracellular Ca2+ response triggered by GSK101 in differentiated osteoblasts.
As a critical second messenger, Ca2+ regulates a variety of cellular signal transduction. CaM/CaMKII signaling is considered to be one of the most important downstream pathways in osteoblasts (Zayzafoon 2006). Upon binding to Ca2+, CaM undergoes structural changes, allowing it to interact and activate various target proteins including CaMKII. Although CaM/CaMKII signaling has been implicated in TRPV4 activation (Loukin et al.2015; Masuyama et al.2012), relatively little is known about the participation of TRPV4 in the regulation of CaMKII activity. In this work, the activation of TRPV4 by its agonist GSK101 induced rapid phosphorylation of CaMKII in differentiated osteoblasts rather than unstimulated cells (Fig. 4). Since there were more TRPV4 channels localized on the membrane in differentiated cells, our results implicated that the activation of CaMKII was relied on the Ca2+ entry mediated by opening TRPV4 at the cell surface. These data together defined a TRPV4 membrane translocation-regulated intracellular Ca2+/CaMKII signaling.
In summary, we demonstrated that osteoblastic differentiation resulted in the translocation of TRPV4 from intracellular space to the plasma membrane. TRPV4 activation by its agonist stimulated Ca2+/CaMKII signaling pathway in osteoblasts, which can be modulated by the membrane abundance of TRPV4 resulted from differentiation. These findings will provide new insights for understanding the mechanisms underlying osteoblastic differentiation and bone remodeling.
Materials and methods
Animals and reagents
New born Sprague–Dawley rats (0–3 day) were obtained from Vital River Laboratory Animal Technology Co., Ltd (Beijing, China, Certification Number: SCXK 2016-0006). Minimum Essential Medium α (α-MEM) and fetal bovine serum (FBS) were from Gibco (USA) and HyClone (USA), respectively. GSK101 and GSK205 were from Millipore (USA). Fura-2/AM was purchased from Biotium (USA). KN-93 was from Selleck (USA). Alizarin red S was from Solarbio (Beijing, China). The rest of reagents, including trypsin, collagenase II, DMSO, bovine serum albumin (BSA), β-glycerophosphate, ascorbic acid, ruthenium red and EGTA were purchased from Sigma-Aldrich (USA).
Osteoblasts isolation, culture and differentiation
Rat calvarial osteoblasts isolation and culture method was described in our earlier work (Hu et al.2014). Briefly, anesthetized new born rats were sacrificed by decapitation. Then, bone skulls were isolated from the soft tissue and digested with 0.1% collagenase II. Calvarial cells were released by repeated digestion with 0.05% trypsin. The isolated osteoblasts were cultured in α-MEM medium containing 10% FBS at 37 °C with 5% CO2. For the differentiation assay, osteoblastic induction was performed by culturing primary calvarial cells in osteogenic medium (OM, α-MEM supplementing with 5 mmol/L β-glycerophosphate and 50 µg/mL ascorbic acid) for the indicated time course (Panupinthu et al.2008). The osteogenic medium was replenished every 3 days.
Measurement of intracellular Ca2+ concentrations ([Ca2+]i)
Osteoblasts were loaded with 5 μmol/L fura-2/AM in Hanks’ balanced salt solution (HBSS) (NaCl 140 mmol/L, KCl 5.4 mmol/L, CaCl2 2 mmol/L, MgCl2 1 mmol/L, glucose 10 mmol/L, and HEPES 10 mmol/L, pH 7.4) for 1 h at 37 °C. After washing extensively with HBSS, [Ca2+]i was measured by calcium imaging system built on an inverted fluorescence microscope (Olympus IX51). The ratiometric Ca2+ indicator fura-2 was alternately excited at 340 nm and 380 nm with a Lambda 10-2 Sutter. Fluorescence images (filtered at 515 nm ± 25 nm) were captured by a CCD camera (CoolSNAP fx-M) and analyzed with MetaFluor software. [Ca2+]i was represented by the ratio of fluorescence intensity at 340 nm/fluorescence intensity at 380 nm (F340/F380). At least three independent experiments were done for each condition. Ca2+-free HBSS solution was made by substituting MgCl2 for CaCl2 at the same concentration with 2 mmol/L EGTA added.
Western blotting for TRPV4 and CaMKII
Osteoblasts were seeded into 10-cm plates (Corning, USA) at 2 × 106 cells per plate and incubated overnight in α-MEM with 10% FBS at 37 °C in prior to various test treatments. Then, the membrane and cytoplasmic protein lysates (for TRPV4) were isolated by a membrane protein isolation kit (Beyotime, China). Briefly, the cells were collected and homogenized to lyse the membrane. After centrifugation at 700 g, the supernatant was collected to remove the nucleus and insufficient lytic cells. The supernatant was then centrifuged at 14,000 g to precipitate cell membranes and the consequent supernatant containing cytoplasmic protein was collected. Finally, membrane protein extraction reagent was used for the extraction of membrane proteins. Total protein lysates (for CaMKII) were isolated by RIPA (Beyotime, China). The concentration of protein was determined using a BCA assay kit (Beyotime, China). After that, aliquots (20 μg/lane) were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane, then blocked non-specific binding sites with 5% BSA at room temperature for 1 h, immunoblotted with anti-TRPV4 (1:200, ACC-034, Alomone labs, Israel), anti-CaMKII and anti-phosphorylated CaMKII (1:1000, 11945S and 12716S, Cell Signaling Technology, USA), as well as mouse anti-β-actin (1:20,000, 60,008-1-lg, Proteintech, USA) overnight at 4 °C, followed by incubation with anti-rabbit/mouse horseradish peroxidase-conjugated secondary antibody (1:1000, A0208/A0216, Beyotime, China). Finally, the ECL detection reagent (Proteintech, USA) was used for visualization in Tanon 5200 MultiImage System.
Immunofluorescence of TRPV4 by 3D super-resolution microscopy
Our super-resolution microscopy is 3D stochastic optical reconstruction microscopy (3D-STORM) with 25 nm lateral resolution and 50 nm axial resolution. Osteoblasts cultured in osteogenic medium for the indicated days were seeded on 12-mm glass cover slips in a 24-well plate (Corning, USA) at 2 × 104 cells per well, incubated for 12 h before immunostaining. After being fixed with 4% paraformaldehyde in phosphate buffered saline (PBS) for 20 min, the samples were permeabilized and blocked in blocking buffer (3% BSA, 0.5% Triton X-100 in PBS) for 20 min. the cells were then incubated with a polyclonal antibody against a peptide corresponding to residues 853–871 of rat TRPV4 (1:100, ACC-034, Alomone labs, Israel) for 1 h at room temperature. After washing for three times, the cells were incubated with the secondary antibody Alexa Fluor® 647 goat anti-rabbit IgG (H + L) (1:400, A21245, ThermoFisher, USA) for 1 h. For 3D-STORM imaging, the samples were mounted on glass slides using an imaging buffer containing 5% glucose, 100 mmol/L cysteamine, 0.8 mg/mL glucose oxidase, and 40 µg/mL catalase in Tris–HCl (pH 7.5) after extensive washing. Fluorescence images were collected via a homebuilt STORM setup based on an inverted optical microscope (Nikon Eclipse Ti-E) equipped with an EMCCD (Andor iXon Ultra 897) (Pan et al.2018). The image stacks with ~50,000 frames per image were used to determine the location of each TRPV4 protein using the localization algorithm described previously (Huang et al.2008), in which the centroid positions and ellipticities of the single-molecule images obtained in each frame were respectively used to deduce the lateral and axial positions of each molecule.
Quantitative real-time PCR (qRT-PCR)
Total RNA was extracted from osteoblasts using the RNAprep pure Cell/Bacteria Kit (Tiangen Biotech, China) following the manufacturer’s instructions. One microgram of isolated RNA was reverse transcribed to cDNA using the PrimeScript™RT Master Mix (Takara, Japan) according to the manufacturer’s protocols. Then, qRT-PCR was conducted with SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara, Japan). Fold gene expression was determined by first normalization to GAPDH, and then normalization to day 0 group. Sequences of gene-specific primers (Table 1) were designed using the Primer 5 (Premier Biosoft, USA) and evaluated with Oligo 7 (Molecular Biology Insights, USA).
Statistical analysis
All data are presented as mean ± standard deviation (SD) from at least three independent experiments. The statistical comparison between two groups was carried out using Student’s t test (Origin 8.1) and the analysis for multiple groups using Dunnett’s test (SPSS 18.0, one-way ANOVA). P < 0.05 was considered to be statistically significant.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (11874231, 11574165 and 31801134), Tianjin Natural Science Foundation (18JCQNJC02000), the PCSIRT (IRT_13R29), and the 111 Project (B07013).
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Fen Hu, Yali Zhao, Zhenhai Hui, Fulin Xing, Jianyu Yang, Imshik Lee, Xinzheng Zhang, Leiting Pan and Jingjun Xu declare that they have no conflict of interest.
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Hu, F., Zhao, Y., Hui, Z. et al. Regulation of intracellular Ca2+/CaMKII signaling by TRPV4 membrane translocation during osteoblastic differentiation. Biophys Rep 5, 254–263 (2019). https://doi.org/10.1007/s41048-019-00100-y
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DOI: https://doi.org/10.1007/s41048-019-00100-y