Abstract
Plant hormones are important for regulating growth, development, and plant-pathogen interactions. Some of them are inhibitory to growth of fungal pathogens but the underlying mechanism is not clear. In this study, we found that hyphal growth of Fusarium graminearum was significantly reduced by high concentrations of IAA and its metabolically stable analogue 2,4-dichlorophenoxyacetic acid (2,4-D). Besides inhibitory effects on growth rate, treatments with 2,4-D also caused significant reduction in conidiation, conidium germination, and germ tube growth. Treatments with 2,4-D had no obvious effect on sexual reproduction but significantly reduced TRI gene expression, toxisome formation, and DON production. More importantly, treatments with 2,4-D were inhibitory to infection structure formation and pathogenesis at concentrations higher than 100 µM. The presence of 1000 µM 2,4-D almost completely inhibited plant infection and invasive growth. In F. graminearum, 2,4-D induced ROS accumulation and FgHog1 activation but reduced the phosphorylation level of Gpmk1 MAP kinase. Metabolomics analysis showed that the accumulation of a number of metabolites such as glycerol and arabitol was increased by 2,4-D treatment in the wild type but not in the Fghog1 mutant. Transformants expressing the dominant active FgPBS2S451D T455D allele were less sensitive to 2,4-D and had elevated levels of intracellular glycerol and arabitol induced by 2,4-D in PH-1. Taken together, our results showed that treatments with 2,4-D interfere with two important MAP kinase pathways and are inhibitory to hyphal growth, DON biosynthesis, and plant infection in F. graminearum.
Highlights
Herbicide 2,4-D was found to inhibit growth, DON biosynthesis, and plant infection in Fusarium graminearum. Whereas ROS accumulation and FgHog1 activation were stimulated, Gpmk1 phosphorylation was reduced by 2,4-D treatments.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
The filamentous ascomycete Fusarium graminearum is a major causal agent of Fusarium head blight (FHB) that affects the production of wheat, barley, and other grains and poses a threat to global food security (Bai and Shaner. 2004; Goswami and Kistler. 2004). This pathogen is a homothallic fungus that forms sexual fruiting bodies known as perithecia to survive on plant debris. It initiates infection of floral tissues of cereal crops with airborne ascospores that are ejected from perithecia. F. graminearum is also a producer of toxic secondary metabolites such as the trichothecene mycotoxin deoxynivalenol (DON) that often contaminates infested grains. As an inhibitor of eukaryotic protein synthesis, DON is an important virulence factor that is essential for spreading infectious growth through the rachis in infected wheat heads (Desjardins. 2003; Proctor et al. 1995a, b).
Genetic and molecular manipulations are relatively efficient in F. graminearum. To date, over a thousand of genes with various biochemical or biological functions, including those encoding protein kinases, transcription factors, and G-protein coupled receptors (GPCRs), have been characterized for their importance in hyphal growth, asexual and sexual reproduction, pathogenesis, secondary metabolism, and stress responses (Jiang et al. 2019; Son et al. 2011; Wang et al. 2011). Interestingly, all three mitogen-activated protein (MAP) kinase pathways play critical roles in regulating plant infection, DON biosynthesis, and sexual reproduction in F. graminearum, with each pathway having its unique functions (Jiang et al. 2018; Zhang et al. 2021). Whereas the MGV1 MAP kinase (MAPK) is important for cell wall integrity and hyphal growth, the GPMK1 pathway is required for penetration and invasive growth. Deletion of MGV1 results in severe growth defects, which can be partially rescued by mutations in the FgHOG1 high osmolarity glycerol (HOG) pathway that plays a key role in responses to hyperosmotic, oxidative, and other stresses (Ren et al. 2019). Like in other fungi, deletion of FgHOG1 or other key components of the HOG pathway confers resistance to fludioxonil, a fungicide that overstimulates the FgHog1 MAPK and accumulation of compatible osmolytes such as glycerol, arabitol, and sorbitol (Van Thuat et al. 2012; Wen et al. 2022; Zheng et al. 2012). Although both Fghog1 and Gpmk1 deletion mutants are slightly reduced in growth rate, the Gpmk1 Fghog1 mgv1 triple mutant grows faster than the mgv1 mutant but is hypersensitive to various abiotic and biotic stresses (Ren et al. 2022).
Besides their functions in regulating growth and development, plant hormones such as auxin, salicylic acid (SA), jasmonic acid (JA), kinetin (KT), gibberellins (GAs), cytokinins, and brassinolides (BRs) play important roles in plant-pathogen interactions (Huang et al. 2020). In infection assays, exogenous application of SA, JA, GA, and Indole-3-acetic acid (IAA, the predominant natural auxin) increase resistance against FHB or spreading of infectious growth (Haidoulis and Nicholson. 2020; Petti et al. 2012). In contrast, treatments with KT disturb defense mechanisms and cell wall fortifications in the host and promote FHB severity (Buhrow et al. 2021). Although the underlying mechanisms are not clear, several plant hormones, including GA, IAA, and cytokinins, exhibit inhibitory effects on fungal growth in axenic cultures (Chanclud and Morel. 2016; Degani et al. 2015; Gupta et al. 2021). F. graminearum is known to produce IAA with the l-tryptophan (L-TRP)-dependent pathway (Luo et al. 2017). However, exogenous IAA is inhibitory to hyphal growth of F. graminearum, which is similar to reports in other plant pathogenic fungi (Degani et al. 2015; Luo et al. 2016; Nicastro et al. 2021; Svoboda et al. 2021). The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) shares structural similarity with IAA and acts as a mimic of auxin. Its inhibitory effects on fungal growth also have been reported in Umbelopsis isabelline (Nykiel-Szymańska et al. 2018).
To determine the effects of different phytohormones or phytohormone-mimicking compounds on F. graminearum and use it to characterize the underlying mechanisms, we assayed the effects of different plant hormones or their analogs, including KT, ABA, IAA, 2,4-D, and GA, on F. graminearum and found that 2,4-D had the highest inhibitory effects on fungal growth. Treatments with 2,4-D also reduced virulence, DON production, and Gpmk1 phosphorylation but induced ROS accumulation and FgHog1 activation. Metabolite profiling analysis showed that the stimulatory effect of 2,4-D on the accumulation of glycerol and arabitol was abolished by deletion of FgHOG1. Overall, our results showed that treatments with 2,4-D are inhibitory to hyphal growth, DON biosynthesis, and plant infection in F. graminearum, partly by interfering with the Gpmk1 and FgHog1 MAPK kinase pathways.
Results
Treatments with high concentrations of 2,4-D and IAA significantly reduce hyphal growth
To determine their effects on F. graminearum, we treated the wild-type strain PH-1 with several phytohormones or phytohormone-mimicking compounds, including 2,4-D, IAA, KT, NAA, gibberellin (GA) and SA. At the concentrations that affected growth in other fungi (Bönnighausen et al. 2019; Luo et al. 2016; Manzo-Valencia et al. 2016), treatments with GA had no significant or obvious effects, but the presence of 1000 µM KT caused a 10.8% reduction in growth rate in comparison with the untreated control. However, treatments with 1000 µM 2,4-D resulted in the most significantly reduction in growth rate of PH-1 (Fig. 1a). Even at 500 µM, 2,4-D was inhibitory to hyphal growth. Furthermore, colonies on cultures with 500 or 1000 µM 2,4-D were less fluffy (Fig. 1a), indicating inhibitory effects on aerial hyphal growth.
2,4-D is a metabolically stable analogue of IAA that regulates cell division, differentiation, and senescence in plants (Romero-Puertas et al. 2022). Because 1000 µM 2,4-D and IAA appeared to be more effective than 500 µM in inhibiting hyphal growth, we treated F. graminearum with higher concentrations of 2,4-D and IAA (Fig. 1b). In the presence of 2,000, 3,000, and 4,000 µM 2,4-D, growth rate was reduced 28.2%, 39.1%, and 46.5%, respectively, in comparison to the untreated control (Fig. 1c), indicating that 2,4-D has a dosage-dependent inhibitory effect on hyphal growth in F. graminearum. Similar dosage-dependent inhibition by IAA also was observed although it was less effective than 2,4-D at the same concentration (Fig. 1b). Growth rate was reduced 22.0%, 24.2% and 35.1% in cultures with 2,000, 3,000, and 4,000 µM IAA, respectively (Fig. 1c). Higher concentrations of 2,4-D also significantly reduced aerial hyphal growth, resulting the formation of colonies with limited aerial hyphae (Fig. 1b).
To further characterize its inhibitory effects, PH-1 was cultivated on PDA plates with lower concentrations of 2,4-D. In the presence of 50 and 200 μM 2,4-D, growth rate was slightly reduced (4.5% and 4.9%, respectively) but there was no obvious reduction in aerial hyphae (Fig. 1b, c). Growth on PDA with 50 and 200 μM IAA was similar to the no treatment control (Fig. 1b). We also measured the dry weights of hyphae grown in liquid YEPD cultures and showed that the presence of 500 and 1000 μM 2,4-D resulted in 18.3% and 28.6% reduction in fungal biomasses, respectively, in comparison with the untreated control (Table 1). These results confirmed the dosage-dependent inhibition of 2,4-D on vegetative growth in F. graminearum.
Treatments with 2,4-D affect conidiation and germination but not sexual reproduction
When the edge of PDA cultures was examined, hyphae grew on the surface had smaller branching angles and were more aggregated in the presence of 2,4-D than the control (Fig. 2a), which may be directly related to reduced growth rate. In CMC cultures, conidiation (asexual reproduction) was significantly reduced by treatments with 500 or 1000 µM 2,4-D (Table 1). When assayed for conidium germination after incubation for 3 h in liquid YEPD medium, less than 5% conidia germinated in the presence of 1000 μM 2,4-D, which was significantly lower than 61.5% in the untreated control (Fig. 2b). Even after incubation for 6 h, 32.5% of conidia were not germinated and the ones germinated had shorter germ tubes in cultures with 1000 μM 2,4-D (Fig. 2c).
We then assayed the effect of 2,4-D on sexual reproduction. Abundant perithecia with asci and ascospores were formed on carrot agar plates with 1000 µM 2,4-D at 8 days post-fertilization (dpf) (Fig. 2d). No obvious differences were observed between mating plates with or without 2,4-D added to the carrot agar medium. These results indicated that treatments with 2,4-D have no obvious effect on sexual reproduction, which differs from its inhibition of vegetative growth and conidiation.
Infectious growth is inhibited by high concentrations of 2,4-D
To determine its effect on fungal infection, flowering wheat heads were drop-inoculated with conidium suspensions of PH-1 containing different concentrations of 2,4-D. At 14 days post-inoculation (dpi), application of 1000 μM 2,4-D alone (no conidium control) did not cause necrosis and had no obvious effect on the development of inoculated wheat kernels (Fig. 3a), which is consistent with its herbicidal effects at concentrations higher than 2 g/L in the field (Wójcik et al. 2020). Nevertheless the virulence of PH-1 was reduced by 2,4-D in a concentration-dependent manner (Table 1). Whereas the presence of 1000 μM 2,4-D completely blocked F. graminearum infection, the disease index was reduced 26.7% and 97.3% by 200 and 500 μM 2,4-D, respectively.
When the rachis tissues next to the inoculated spikelets were examined, abundant infectious hyphae were observed in the control samples without 2,4-D. In samples treated with 200 or 500 μM 2,4-D, infectious hyphae were significantly reduced, particularly in the latter (Fig. 3b). Infectious hyphae were rarely observed in rachis tissues of samples treated with 1000 μM 2,4-D (Fig. 3b), which is consistent with the disease index being less than 0.5. These results indicate that 2,4-D is inhibitory to infectious growth and infection of wheat head tissues.
In infection assays with wheat coleoptiles, treatments with 200 and 500 μM 2,4-D also significantly reduced the virulence of PH-1 (Fig. 3c). The length of necrosis lesions was significantly reduced in the presence of 200 or 500 μM 2,4-D. Necrosis on wounded wheat leaf sheaths was rarely observed when inoculated with conidium suspensions with 1000 μM 2,4-D (Fig. 3c), which is consistent with results from wheat head infection assays. These results indicated that the application of high concentrations of 2,4-D inhibits the growth and spreading of infectious hyphae and reduces fungal virulence.
Biosynthesis of DON is reduced by treatments with 2,4-D
Because DON is an important virulence factor in F. graminearum (Proctor et al. 1995a, b), we also assayed the effect of 2,4-D on DON biosynthesis in liquid trichothecene biosynthesis (LTB) medium (Gardiner et al. 2009). Treatments with 100 and 200 μM 2,4-D resulted in 59.1% and 70.2% reduction in DON production, respectively, in comparison with the ethanol solvent control (Fig. 4a). In the presence of 500 or 1000 μM 2,4-D, DON was barely detectable or only a low amount of DON was detected in 7-day-old LTB cultures (Fig. 4a).
To confirm the inhibitory effects of 2,4-D on DON biosynthesis, we selected the TRI5, TRI1, and TRI12 genes for qRT-PCR assays. Whereas TRI5 encodes the trichodiene synthase that catalyzes the first step of trichothecene biosynthesis, TRI12 encodes an MFS transporter that functions as a trichothecene efflux pump (Alexander et al. 1999; Hohn and Beremand. 1989; Wang et al. 2018). The Tri1 calonectrin oxygenase is involved in late steps of trichothecene biosynthesis and it is a component of the toxisome (Menke et al. 2013). In qRT-PCR assays with RNA isolated from LTB cultures, the expression of the TRI5 trichodiene synthetase gene was reduced over 1,428-fold by 500 μM 2,4-D in comparison with the ethanol solvent control (Fig. 4b). The expression level of the TRI1 calonectrin oxygenase and TRI12 transporter genes (Menke et al. 2012) also was significantly reduced by 2,4-D (Fig. 4b). Microscopical examination showed that the formation of intercalary swollen hyphal compartments in LTB cultures was significantly reduced by 500 μM 2,4-D (Fig. 4c). We also generated a transformant of PH-1 expressing the TRI1-GFP construct. Whereas GFP signals and toxisome formation were observed in untreated samples, treatments with 500 μM 2,4-D inhibited Tri1-GFP expression and toxisome formation (Fig. 4d). Taken together, 2,4-D is inhibitory to DON biosynthesis, likely by inhibiting TRI gene expression and toxisome formation.
Treatments with 2,4-D cause oxidative stress
Treatments with 2,4-D have been reported to induce membrane and oxidative stress in plants and the fungus Umbelopsis isabellina (Bernat et al. 2018a, b; Grossmann. 2010). To determine whether 2,4-D induced ROS in F. graminearum, we assayed ROS accumulation in conidia and germlings treated with 2,4-D by staining with dichlorodihydrofluorescein diacetate (DCFH-DA), a cell-permeable indicator for ROS (Liu et al. 2010). Intense green fluorescence signals were observed in the cytoplasm of germ tubes treated with 2,4-D but not in the control samples (Fig. 5a). In addition, we assayed the level of fluorescence signals based on excitation wavelength at 480 nm and emission wavelength at 530 nm (Oparka et al. 2016). The fluorescent signal in hyphae treated with 2,4-D was 2.9-fold higher than that of the untreated control (Fig. 5b). These observations showed that treatments with 2,4-D promoted the production of intracellular ROS in F. graminearum.
The activation of FgHog1 MAP kinase is stimulated by 2,4-D
Like in other fungi, the HOG pathway plays a critical role in regulating stress responses in F. graminearum (Zhang et al. 2021). Therefore, we assayed the effect of 2,4-D on the expression and activation of the FgHog1 MAP kinase. In proteins isolated from vegetative hyphae of PH-1 treated with 500 µM 2,4-D for 30 min, the expression of FgHog1 was not affected but its activation was significantly increased when detected with an anti-TpGY antibody (Fig. 6a). In repeated tests, the phosphorylation level of FgHog1 was increased over fourfold in samples treated with 2,4-D (Fig. 6b), suggesting a stimulatory effect on the activation of the FgHog1 MAPK pathway. In comparison with treatments with 50, 500, and 1,000 µM 2,4-D, the stimulation of FgHog1 phosphorylation appeared to be concentration dependent (Fig. S1).
Because over-stimulation of FgHog1 has a detrimental effect on hyphal growth in F. graminearum (Zhang et al. 2021), we assayed the inhibitory effect of 2,4-D on the Fghog1 mutant HG6 that had a reduced growth rate compared to PH-1 (Zheng et al. 2012). In PDA cultures with 2000 μM 2,4-D, the growth inhibition rate on the Fghog1 mutant was 11.8%, which was significantly less than 28.6% on PH-1 (Fig. 6c, d), suggesting increased tolerance against 2,4-D in the mutant. As the control, the Fghog1/FgHOG1 transformant HGC1 had similar sensitivities to 2,4-D as PH-1 (Fig. 6c). The growth inhibition rate was 27.3% in the complemented transformant (Fig. 6d). These results indicated that overstimulation of FgHog1 may play a role in conferring sensitivities to 2,4-D in F. graminearum.
Treatments with 2,4-D reduces the phosphorylation level of Gpmk1 MAP kinase
Because of the stimulatory effects of 2,4-D on FgHog1, we also assayed its effects on the expression and activation of Gpmk1 and Mgv1, two MAP kinases with the TEY dual phosphorylation site. When detected with an anti-Erk2 antibody, the expression of Gpmk1 and Mgv1 was not affected by 2,4-D treatment. However, when assayed with the anti-TpEY antibody, the phosphorylation of Gpmk1 was significantly reduced by 2,4-D although it had no obvious effects on Mgv1 activation (Fig. 6a). In comparison with the untreated control, treatments with 2,4-D resulted in over sixfold reduction in the phosphorylation of Gpmk1 (Fig. 6b).
In F. graminearum, the Gpmk1 deletion mutant is non-pathogenic and its ortholog has a conserved function in regulating plant infection processes in different fungal pathogens (Jenczmionka et al. 2003; Xu. 2000). The inhibitory effects of 2,4-D on Gpmk1 activation may contribute to its inhibitory effect on plant infection. To test this hypothesis, we assayed the effect of 500 µM 2,4-D on the formation of infection structures on wheat lemmas. When examined by scanning electron microscopy (SEM), PH-1 treated with 2,4-D, similar to the Gpmk1 deletion mutant, formed smaller infection cushions with less complex structures than the untreated control (Fig. 7a). However, treatments with 2,4-D appeared to have no obvious effects on the number of infection cushions formed by the wild type (Fig. 7b). Although infection cushions were not fully developed in the gpmk1 mutant, deletion of GPMK1 also lacked obvious effects on the number of infection cushions developed on lemmas (Fig. 7b). In addition, treatments with 2,4-D appeared to impair plant penetration and infectious growth in F. graminearum. In inoculated wheat lemmas, the growth of coralloid hyphae was significantly reduced by 2,4-D in comparison with the untreated control (Fig. 7c). Therefore, the inhibitory effect of 2,4-D on plant infection may be related to its inhibition on Gpmk1 activation in F. graminearum.
The FgHog1 pathway regulates the accumulation of glycerol and sorbitol stimulated by 2,4-D
To determine the effect of 2,4-D on metabolism in F. graminearum, vegetative hyphae of PH-1 harvested from 20 h YEPD cultures were treated with 500 μM 2,4-D for 4 h. Metabolites were then extracted from treated hyphae after grinding in liquid nitrogen, desiccated, and derivatized with trimethylsilylimidazole (TMSI) and trimethylchlorosilane (TMCS) before GC–MS analysis as described (Smith and Bluhm. 2011; Zheng et al. 2012). Among the 96 metabolites identified (Table S1), 35 of them were significantly induced (over twofold, p < 0.05) by treatments with 500 μM 2,4-D in comparison with the control (Fig. 8), including glycerol and arabitol that are known to be accumulated in response to hyperosmotic stress under the control of the FgHog1 MAP kinase pathway in F. graminearum (Zheng et al. 2012).
To determine whether 2,4-D stimulates the accumulation of glycerol and other compatible solvents via the FgHog1 pathway, we compared metabolites in the Fghog1 mutant treated with 2,4-D. Among the 35 metabolites significantly stimulated by 2,4-D in PH-1, only 2 of them had increased over twofold levels in the Fghog1 mutant after 2,4-D treatment (Fig. 8). Unlike in PH-1, the accumulation of glycerol and arabitol was not stimulated by 2,4-D in the Fghog1 mutant although sucrose accumulation was not affected by FgHOG1 deletion (Fig. 9a). Furthermore, we generated the FgPBS2S451D T455D allele to mimic dominant active mutation and transformed it into PH-1. FgPBS2 encodes the MAP kinase kinase that activates the FgHog1 MAP kinase. In comparison with PH-1, the FgPBS2S451D T455D transformants were less sensitive to 2,4-D treatment (Fig. 9b). In PDA cultures with 1000 μM 2,4-D, the growth inhibition rate was 9.4% in the FgPBS2S451D T455D transformant, which was significantly less than 23.6% in PH-1. Among the 35 metabolites significantly stimulated by 2,4-D in PH-1, 13 of them had increased over twofold levels in the FgPBS2S451D T455D transformant in comparison with the untreated PH-1 control (Fig. 8). Taken together, these results suggested that stimulating the activation of the FgHog1 MAPK pathway likely contributes partially to changes in metabolites caused by 2,4-D in F. graminearum.
Genes affected in hyphae by 2,4-D treatment
To determine the effect of 2,4-D treatment on gene expression, we isolated RNA from hyphae of PH-1 treated with 0.4% ethanol (solvent control) or 500 µM 2,4-D for 2 h and identified differentially expressed genes (DEGs, ≥ twofold changes) by RNA-seq analysis. In comparison with the control, 1,339 DEGs were up-regulated and 1,013 DEGs were down-regulated in PH-1 treated with 2,4-D (Table S2). KEGG enrichment analysis showed that DEGs up-regulated by 2,4-D were highly enriched for genes involved in carbohydrate, amino acid, and energy metabolism (Fig. S2). Consistent with its stimulation of ROS accumulation, the expression of NOXA and NOXC (Wang et al. 2014) was up-regulated more than twofold by 2,4-D. In contrast, the top two enriched categories in down-regulated DEGs are related to ‘cell growth and death’ and ‘protein folding, sorting and degradation’ (Fig. S2), which may be related to the oxidative stress and reduced growth rate caused by treatments with 2,4-D. Homologs of yeast BUB2, CDC20, REC8, CDC6, CLB, RAD53 and ORC1/2/5 that are involved in mitosis, septation, polarity establishment, and DNA replication are among the down-regulated DEGs by 2,4-D in the ‘cell growth and death’ category, which also include two Gα subunits known to regulate signal perception, propagation and growth in F. graminearum (Yu et al. 2008).
The intracellular glycerol level can be influenced by different metabolic pathways related to glycerol synthesis and metabolism (Foster et al. 2017). Although the expression of the glycerol-3-phosphate phosphatase gene (FGRRES_07096) was not affected, the haloacid dehalogenase (FGRRES_06381) and glycerol-phosphate dehydrogenase (FGRRES_03249) genes were up-regulated 1.9-fold and 14-fold, respectively, by 2,4-D treatment. In addition, the expression of triacyl/monoacyl-glycerol lipase genes FGRRES_02015, FGRRES_01603, and FGRRES_02944 was up-regulated over twofold by 2,4-D, suggesting a possible role of triglyceride breakdown in glycerol accumulation stimulated by this herbicide. For increased accumulation of arabitol, the expression of arabitol dehydrogenase genes was not affected but the ribulokinase gene (FGRRES_01430) was upregulated over twofold by 2,4-D treatment.
Discussion
Plant hormones regulate a variety of physiological processes including growth, development, biotic and abiotic stress response. In this study, we found that treatments with KT, GA, and SA at concentrations reported to be inhibitory to growth in other fungi (Bönnighausen et al. 2019; Luo et al. 2016; Manzo-Valencia et al. 2016; Takeda et al. 2015) had no obvious effects on hyphal growth in F. graminearum. However, treatments with IAA and its analog 2,4-D were inhibitory to hyphal growth in a dose-dependent manner, with the latter being more metabolically stable and effective on F. graminearum. Significant inhibition on hyphal growth was observed when the concentration of 2,4-D was over 500 μM, but only when IAA was over 2000 μM. Even at 50 μM, 2,4-D still had a minor inhibitory effect on fungal growth. The growth rate of F. graminearum was not affected by the presence of 50 μM IAA, a concentration higher than its normal physiological concentration as a plant hormone (Kidd et al. 2011).
Although growth inhibition by IAA at the concentration of 500 and 1000 μM has been reported in other fungi such as U. maydis (Reineke et al. 2008), inhibitory effects of 2,4-D on fungal growth has only been reported in U. isabelline and growth rate was only slightly reduced after treatments for 120 h (Nykiel-Szymańska et al. 2018). In this study, we showed that treatments with 2,4-D not only inhibited hyphal growth but also reduced conidium germination, germ tube growth, and DON production. Whereas its inhibition on germ tube growth may be directly related to inhibitory effects of 2,4-D on polarized tip growth, conidium germination involves the establishment of polarized growth, which may be also disturbed by 2,4-D treatment. One of the down-regulated DEGs by 2,4-D is FGRRES_05216, an ortholog of yeast PEA2 that is involved in polarity establishment. The expression of FGRRES_05766, an ortholog of yeast CDC43, also was reduced over twofold. For its inhibitory effect on DON biosynthesis, 2,4-D significantly reduced the expression of TRI5 and other TRI genes as well as toxisome formation. Like in eukaryotes, Rho GTPases play important roles in morphogenesis and conidiation in F. graminearum (Zhang et al. 2013). Two of its GTPases, RHO1 and RHO4, were down-regulated over twofold by 2,4-D treatment.
Treatments with higher concentrations of 2,4-D were inhibitory to plant infection in F. graminearum, which has not been reported in other fungi. In samples treated with over 500 μM 2,4-D, infectious hyphae were significantly reduced in rachis tissues, indicating inhibitory effects of 2,4-D on infectious growth. However, most of the inoculated wheat kernels had no FHB symptoms in the presence of 1000 μM 2,4-D, indicating that the initial infection processes are also inhibited. Besides of its inhibitory effects on hyphal growth that may directly contribute to reduced virulence, 2,4-D was found to inhibit the activation of Gpmk1 MAP kinase. Like in other plant pathogenic fungi, this MAP kinase pathway regulates various infection processes and is essential for plant infection in F. graminearum (Jenczmionka et al. 2003). Significant reduction in Gpmk1 activation by 2,4-D may be another critical factor for its inhibitory effects on fungal pathogenesis. In fact, treatments with 2,4-D had similar effects on infection cushion formation and growth of coralloid hyphae in wheat lemma tissues as deletion of GPMK1. Although the underlying mechanism is not clear, a reduction in Gpmk1 activation by 2,4-D may be caused by overstimulation of FgHog1 because MAP kinase pathways are known to crosstalk (Zhang et al. 2021). Nevertheless, it is also possible that Gpmk1 activation is reduced by 2,4-D via its other targets, independent of FgHog1, in F. graminearum.
In U. isabelline, treatments with 2,4-D induce membrane and oxidative stress (Bernat et al. 2018a, b). In F. graminearum, treatments with 2,4-D induced ROS accumulation and increased the phosphorylation level of FgHog1 MAP kinase. Consistent with the overactivation of FgHog1, the intracellular level of glycerol and arabitol was increased in response to 2,4-D treatment in the wild type. In F. graminearum and other fungi, the FgHog1 MAP kinase is important for regulating response to oxidative and hyperosmotic stresses as well as glycerol synthesis (Zhang et al. 2021). In comparison with the wild type, the inhibitory effect of 2,4-D on hyphal growth was reduced in the Fghog1 deletion mutant. Furthermore, the FgPBS2S451D T455D transformant was less susceptible to 2,4-D and had increased accumulation of glycerol and arabitol that were stimulated by 2,4-D in PH-1. Thus, overactivation of the FgHog1 MAP kinase by 2,4-D may contribute partially to its inhibitory effects on F. graminearum. Phenylpyrrol fungicides such as fludioxonil overstimulate the activation of the HOG pathway and cause hyphal tip burst due to the accumulation of high concentrations of compatible solvents such as glycerol (Zhang et al. 2002). Although hyphal growth and branching were affected, hyphal tip burst was not observed by 2,4-D treatment in F. graminearum, which may be due to only relatively low concentrations of intracellular glycerol were accumulated in hyphae treated with 2,4-D in comparison with fludioxonil treatment (Zhang et al. 2002).
Overall, our results showed that treatments with 2,4-D were inhibitory to F. graminearum on growth, asexual reproduction, DON production, and pathogenesis, which may be directly related to its overactivation of FgHog1 and suppression of Gpmk1, two MAP kinases important for these infection and developmental processes (Jenczmionka et al. 2003; Zheng et al. 2012). However, besides its effects on these two MAP kinase pathways, 2,4-D likely has other targets in F. graminearum, as treatments with 2,4-D have been shown to increase or suppresse the expression of over 2,000 genes. Many of these genes are functionally unknown or their orthologs are not known to be related to FgHog1 or Gpmk1 MAP kinase pathways in fungi. For examples, FgHyd4 and MYT3 genes that are important for hyphal growth or conidiation in F. graminearum were reduced over twofold by 2,4-D treatment (Kim et al. 2014; Shin et al. 2022). Treatments with 4,000 µg/ml 2,4-D also resulted in more severe colonial and aerial hyphal growth than deletion of FgHOG1 or GPMK1. Therefore, it will be important to generate and characterize mutants that become resistant or tolerant to 2,4-D to better understand the underlying mechanisms for the inhibitory effects of this herbicide on fungal growth and plant infection.
Materials and methods
Strains and cultural conditions
The wild-type strain PH-1 (Cuomo et al. 2007), Fghog1 mutant HG6 (Zheng et al. 2012), and transformants generated in this study were routinely cultured on potato dextrose agar (PDA) at 25℃ (Yin et al. 2020). To determine their inhibitory effects on fungal growth, 2,4-D (Coolaber, China), IAA (Aladdin, China), NAA (Aladdin, China), KT (Aladdin, China), GA (Aladdin, China), and SA (Aladdin, China) were added to the final concentration of 500 and 1000 µM to PDA plates. IAA and 2,4-D were dissolved in 100% ethanol as the stock solutions and assayed with 0.4% ethanol (v/v) as the untreated control. Hyphae collected from YEPD cultures treated with 2,4-D for 24 h were lyophilized for 24 h in a Heto Powerdryer PL3000 (Thermo, USA) and measured for dry weights as described (Mao et al. 2020). Conidiation was assayed in 5-day-old CMC as described (Hou et al. 2002). For sexual reproduction, aerial hyphae of 7-day-old carrot agar cultures with or without 1000 µM 2,4-D were pressed down with 0.1% Tween 20 and further incubated at 25℃ under black light. Perithecium formation and production of cirrhi were examined at 8 days post-fertilization. Protoplast preparation, transformation, and selection of transformants resistant to hygromycin were performed as described (Hou et al. 2002; Zhou et al. 2010).
Generation of the TRI1-GFP and FgPBS2 S451D T455D transformants
The TRI1 gene was amplified with primer pairs TRI1-GFP-F/TRI1-GFP-R (Table S3) and fused to GFP by overlapping PCR. The resulting TRI1-GFP construct was transformed into protoplasts of PH-1 to obtain TRI1-GFP transformants. To introduce the S451D and T455D mutations in FgPBS2 that were equivalent to the S514D and T518D mutations in yeast PBS2 (Tatebayashi et al. 2020), we first used overlapping PCR to introduce the S451D and T455D mutations with primer pairs FgPBS2-1F/FgPBS2-1R and FgPBS2-2F/FgPBS2-2R (Table S3). The resulting PCR products were cloned into plasmid pKNTG (Lou et al. 2021) and transformed into PH-1. Transformants resistant to hygromycin were verified by PCR and sequencing analysis for carrying the transforming FgPBS2S451D T455D allele.
Plant infection assays
Wheat heads of cultivar Xiaoyan22 (Kang et al. 2008) were inoculated with 10 μl of conidia suspensions (2 × 105 spores/ml in sterile distilled water) with 0, 100, 200, 500, or 1000 µM 2,4-D as described (Jiang et al. 2019). Inoculated spikelets were examined at 14 dpi for diseased kernels to estimate the disease index. For assaying infectious growth, infected rachis tissues were sampled at 5 dpi and embedded in Spurr resin after fixation and dehydration as described (Jenczmionka et al. 2003; Jiang et al. 2016). Thick Sects. (1 mm) were stained with 0.5% (w/v) toluidine blue and examined with an Olympus BX-53 microscope (Olympus, Japan). To assay infection cushion formation, inoculated lemmas were sampled at 3 dpi, fixed with 4% (v/v) glutaraldehyde, and coated with gold–palladium before examination by scanning electron microscopy JSM-6360LV (JEOL, Japan) as described (Jiang et al. 2019). To observe infectious growth in lemma tissues, inoculated lemmas were sampled at 3 dpi, stained with Alexa Fluor 488, and examined by laser scanning confocal microscope LSM880 (Zeiss, Germany) as described (Becker et al. 2016). For infection assays with wheat coleoptiles, Xiaoyan22 seedlings were cut off the top portion and inoculated with conidium suspensions with 2,4-D at the wound sites (Wu et al. 2005). Necrotic lesions on leaf sheaths were examined at 7 dpi. Inoculations with 1000 μM 2,4-D without conidia suspensions was the control to show no detectable phytotoxic effects.
Assays for the intracellular ROS level
Intracellular ROS in hyphae was assayed with the reactive oxygen species assay kit purchased from Beyotime Institute of Biotechnology (Haimen, Jiangsu, China) following the instruction provided by the manufacturer (Jia et al. 2006). In brief, hyphae of PH-1 cultured in YEPD for 8 h were treated with 500 µM 2,4-D or 0.4% ethanol for 30 min. Treated hyphae were harvested by filtration and resuspended in 10 μM 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA). After incubation for 30 min at 37 °C, hyphae were examined by epifluorescence microscopy. The fluorescence intensity of intracellular ROS was measured with the microplate reader Victor Nivo (PerkinElmer, USA) with the excitation wavelength at 480 nm and emission wavelength at 530 nm.
MAPK phosphorylation assays
Vegetative hyphae of PH-1 were harvested from 12 h YEPD cultures and treated with 500 μM 2,4-D or 0.4% ethanol (v/v) for 30 min. Total proteins were then isolated with protein lysis buffer containing protease inhibitor cocktail (Sigma-Aldrich, USA), phosphatase inhibitor cocktails 2 (Sigma-Aldrich, USA) and phosphatase inhibitor cocktails 3 (Sigma-Aldrich, USA) and separated on 10% PAGE gels as described (Zhang et al. 2018). Western blots were detected with the anti-TpGY phosphorylation-specific (for detection of phosphorylated FgHog1 with the TGY dual phosphorylation site), anti-FgHog1, anti-TpEY phosphorylation-specific (for detection of phosphorylated Gpmk1 and Mgv1 MAP kinases with the TEY dual phosphorylation site), and anti-ERK2 antibodies (Cell Signaling Technology, USA) as described (Zhang et al. 2018, 2017) for assaying the phosphorylation and expression levels of FgHog1, Gpmk1, and Mgv1 MAP kinases. Detection with an anti-β-tubulin (anti-tub2) antibody was used as the protein loading control as described (Wang et al. 2019).
Assays for the effects of 2,4-D on DON biosynthesis and TRI gene expression
For assaying its effects on DON production, 2,4-D was added to the final concentrations of 100, 200, 500, and 1000 µM to 2 ml LTB (Liquid trichothecene biosynthesis) cultures with 1 × 104 conidia/ml as described (Liu et al. 2019). After incubation at 25 °C for 7 d, DON was extracted from LTB cultures and analyzed with a GCMS-QP2010 (Shimadzu, Japan) as described (Liang et al. 2021). Hyphae from 3-day-old LTB cultures were examined for swollen hyphal compartments and toxisome formation (Menke et al. 2012). For assaying TRI gene expression, RNA was isolated from hyphae collected from 3-day-old LTB cultures with the TRIzol reagent (Invitrogen, USA). The FastKing RT Kit (TIANGEN, China) was used to assay the expression of TRI genes and actin gene of F. graminearum (as the internal control) with the CFX96 Real-Time System (Bio-RAD, USA) (Jiang et al. 2016). The mean and standard deviation of relative expression levels were calculated with data from three independent biological replicates.
RNA-seq analysis
For RNA-seq analysis, vegetative hyphae harvested from 12-h YEPD cultures were resuspended in 500 µM 2,4-D or 0.4% ethanol and incubated for 2 h. RNA samples were then isolated with the TRIzol reagent. RNA-seq libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit (NEB, USA) following the manufacturer’s instructions and sequenced with Illumina HiSeq 2500 with the paired-end 2 × 150 bp model at the Novogene Bioinformatics Institute (Beijing, China). The resulting RNA-seq reads were mapped onto the reference F. graminearum genome (Lu et al. 2022) by HISAT2 (Kim et al. 2015) and analyzed for the number of reads mapped to each gene with featureCounts (Liao et al. 2014). The edgeRun package with the exactTest function (Dimont et al. 2015) was used to analyze differentially expressed genes. Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analyses of DEGs were conducted in R software using the package GO plot (Sun et al. 2021).
Analysis for metabolic fingerprinting
Hyphae harvested from 20 h YEPD cultures were resuspended in YEPD medium with 500 µM 2,4-D or 0.4% ethanol (control) and further incubated for 4 h (Zheng et al. 2012). Treated hyphae were collected by filtration, washed 3 times with sterilized distilled water, and lyophilized for 24 h with Heto Powerdryer PL3000. After grinding in liquid nitrogen, 50 mg hyphal powders were resuspended in 1.6 ml methanol (w/v) and homogenized by sonification for 30 min. The supernatant (1 ml) was transferred to a new tube after centrifugation at 3500 rpm for 10 min and dried in a Savant ISS110 SpeedVac (Thermo, USA). After derivatization with 50 μl TMSI and TMCS (100: 1) (Sigma, USA) as described (Smith and Bluhm. 2011), each sample was mixed with 1 ml isooctane and 1 ml ultrapure water. The top organic phase was isolated after centrifugation and filtered with 0.22 μm nylon membrane (Shimadzu, Japan). For each sample, 1 μl was injected into a GCMS-QP2010 Ultra (Shimadzu, Japan) coupled with a Rxi-5 ms column (30 m × 0.25 mm × 0.25 μm). Helium was used as the carrier gas at a constant flow rate of 1 ml/min with the injector temperature of 260℃ and a split ratio of 30:1 (Zheng et al. 2012). The GC–MS detection conditions were as described (Smith and Bluhm. 2011). Shimadzu software GCMS Postrun Analysis was used for comparison of chromatograms, integration of peaks, and compound identification as described (Barreto et al. 2011). Heatmap visualization of log2-transformed compounds peak areas was performed using online software of MetaboAnalyst 5.0 (https://www.metaboanalyst.ca/) (Spicer et al. 2017).
Availability of data and materials
The RNA-Seq data used in this study are available in National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) and can be accessed with Bio-Project ID PRJNA956133.
References
Alexander NJ, McCormick SP, Hohn TM (1999) TRI12, a trichothecene efflux pump from Fusarium sporotrichioides: gene isolation and expression in yeast. Mol Gen Genet 261:977–984. https://doi.org/10.1007/s004380051046
Bai G, Shaner G (2004) Management and resistance in wheat and barley to fusarium head blight. Annu Rev Phytopathol 42:135–161. https://doi.org/10.1146/annurev.phyto.42.040803.140340
Barreto MC, Vilas Boas L, Carneiro LC, San Romão MV (2011) Volatile compounds in samples of cork and also produced by selected fungi. J Agric Food Chem 59:6568–6574. https://doi.org/10.1021/jf200560e.
Becker M, Becker Y, Green K, Scott B (2016) The endophytic symbiont Epichloë festucae establishes an epiphyllous net on the surface of Lolium perenne leaves by development of an expressorium, an appressorium-like leaf exit structure. New Phytol 211:240–254. https://doi.org/10.1111/nph.13931.
Bernat P, Nykiel-Szymańska J, Gajewska E, Różalska S, Stolarek P, Dackowa J, Słaba M (2018) Trichoderma harzianum diminished oxidative stress caused by 2,4-dichlorophenoxyacetic acid (2,4-D) in wheat, with insights from lipidomics. J Plant Physiol 229:158–163. https://doi.org/10.1016/j.jplph.2018.07.010.
Bernat P, Nykiel-Szymańska J, Stolarek P, Słaba M, Szewczyk R, Różalska S (2018) 2,4-dichlorophenoxyacetic acid-induced oxidative stress: Metabolome and membrane modifications in Umbelopsis isabellina, a herbicide degrader. PLoS One 13:e0199677. https://doi.org/10.1371/journal.pone.0199677.
Bönnighausen J, Schauer N, Schäfer W, Bormann J (2019) Metabolic profiling of wheat rachis node infection by Fusarium graminearum-decoding deoxynivalenol-dependent susceptibility. New Phytol 221:459–469. https://doi.org/10.1111/nph.15377.
Buhrow LM, Liu Z, Cram D, Sharma T, Foroud NA, Pan Y, Loewen MC (2021) Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge. BMC Genomics 22:798. https://doi.org/10.1186/s12864-021-08069-0.
Chanclud E, Morel JB (2016) Plant hormones: a fungal point of view. Mol Plant Pathol 17:1289–97. https://doi.org/10.1111/mpp.12393
Cuomo CA, Güldener U, Xu JR, Trail F, Turgeon BG, Di Pietro A et al (2007) The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization. Science 317:1400–1402. https://doi.org/10.1126/science.1143708.
Degani O, Drori R, Goldblat Y (2015) Plant growth hormones suppress the development of Harpophora maydis, the cause of late wilt in maize. Physiol Mol Biol Plants 21:137–149. https://doi.org/10.1007/s12298-014-0265-z
Desjardins AE (2003) Gibberella from A (venaceae) to Z (eae). Annu Rev Phytopathol 41:177–198. https://doi.org/10.1146/annurev.phyto.41.011703.115501
Dimont E, Shi J, Kirchner R, Hide W (2015) edgeRun: an R package for sensitive, functionally relevant differential expression discovery using an unconditional exact test. Bioinformatics 31:2589–90. https://doi.org/10.1093/bioinformatics/btv209.
Foster AJ, Ryder LS, Kershaw MJ et al (2017) The role of glycerol in the pathogenic lifestyle of the rice blast fungus Magnaporthe oryzae. Environ Microbiol 19:1008–1016. https://doi.org/10.1111/1462-2920.13688
Gardiner DM, Kazan K, Manners JM (2009) Nutrient profiling reveals potent inducers of trichothecene biosynthesis in Fusarium graminearum. Fungal Genet Biol 46:604–613. https://doi.org/10.1016/j.fgb.2009.04.004
Goswami RS, Kistler HC (2004) Heading for disaster: Fusarium graminearum on cereal crops. Mol Plant Pathol 5:515–525. https://doi.org/10.1111/j.1364-3703.2004.00252.x
Grossmann K (2010) Auxin herbicides: current status of mechanism and mode of action. Pest Manag Sci 66:113–120. https://doi.org/10.1002/ps.1860
Gupta R, Anand G, Pizarro L, Laor D, Kovetz N, Sela N, Yehuda T, Gazit E, Bar M (2021) Cytokinin inhibits fungal development and virulence by targeting the cytoskeleton and cellular trafficking. mBio 12:e0306820. https://doi.org/10.1128/mBio.03068-20.
Haidoulis JF, Nicholson P (2020) Different effects of phytohormones on Fusarium head blight and Fusarium root rot resistance in Brachypodium distachyon. bioRxiv 15:335–344. https://doi.org/10.1080/17429145.2020.1820592
Hohn TM, Beremand PD (1989) Isolation and nucleotide sequence of a sesquiterpene cyclase gene from the trichothecene-producing fungus Fusarium sporotrichioides. Gene 79:131–138. https://doi.org/10.1016/0378-1119(89)90098-x
Hou Z, Xue C, Peng Y, Katan T, Kistler HC, Xu JR (2002) A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection. Mol Plant Microbe Interact 15:1119–1127. https://doi.org/10.1094/mpmi.2002.15.11.1119.
Huang S, Zhang X, Fernando WGD (2020) Directing trophic divergence in plant-pathogen interactions: antagonistic phytohormones with no doubt? Front Plant Sci 11:600063. https://doi.org/10.3389/fpls.2020.600063
Jenczmionka NJ, Maier FJ, Lösch AP, Schäfer W (2003) Mating, conidiation and pathogenicity of Fusarium graminearum, the main causal agent of the head-blight disease of wheat, are regulated by the MAP kinase gpmk1. Curr Genet 43:87–95. https://doi.org/10.1007/s00294-003-0379-2.
Jia SJ, Jiang DJ, Hu CP, Zhang XH, Deng HW, Li YJ (2006) Lysophosphatidylcholine-induced elevation of asymmetric dimethylarginine level by the NADPH oxidase pathway in endothelial cells. Vascul Pharmacol 44:143–148. https://doi.org/10.1016/j.vph.2005.09.005.
Jiang C, Zhang C, Wu C, Sun P, Hou R, Liu H, Wang C, Xu JR (2016) TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum. Environ Microbiol 18:3689–3701. https://doi.org/10.1111/1462-2920.13279.
Jiang C, Zhang X, Liu H, Xu JR (2018) Mitogen-activated protein kinase signaling in plant pathogenic fungi. PLoS Pathog 14:e1006875. https://doi.org/10.1371/journal.ppat.1006875.
Jiang C, Cao S, Wang Z, Xu H, Liang J, Liu H, Wang G, Ding M, Wang Q, Gong C, Feng C, Hao C, Xu JR (2019) An expanded subfamily of G-protein-coupled receptor genes in Fusarium graminearum required for wheat infection. Nat Microbiol 4:1582–1591. https://doi.org/10.1038/s41564-019-0468-8.
Kang Z, Buchenauer H, Huang L, Han Q, Zhang H (2008) Cytological and immunocytochemical studies on responses of wheat spikes of the resistant Chinese cv. Sumai 3 and the susceptible cv. Xiaoyan 22 to infection by Fusarium graminearum. Eur J Plant Pathol 120:383–396. https://doi.org/10.1007/s10658-007-9230-9.
Kidd BN, Kadoo NY, Dombrecht B, Tekeoglu M, Gardiner DM, Thatcher LF, Aitken EA, Schenk PM, Manners JM, Kazan K (2011) Auxin signaling and transport promote susceptibility to the root-infecting fungal pathogen Fusarium oxysporum in Arabidopsis. Mol Plant Microbe Interact 24:733–748. https://doi.org/10.1094/mpmi-08-10-0194.
Kim Y, Kim H, Son H, Choi GJ, Kim JC, Lee YW (2014) MYT3, a Myb-like transcription factor, affects fungal development and pathogenicity of Fusarium graminearum. PLoS One 9:e94359. https://doi.org/10.1371/journal.pone.0094359.
Kim D, Langmead B, Salzberg SL (2015) HISAT: a fast spliced aligner with low memory requirements. Nat Methods 12:357–360. https://doi.org/10.1038/nmeth.3317
Liang J, Fu X, Hao C, Bian Z, Liu H, Xu JR, Wang G (2021) FgBUD14 is important for ascosporogenesis and involves both stage-specific alternative splicing and RNA editing during sexual reproduction. Environ Microbiol 23:5052–5068. https://doi.org/10.1111/1462-2920.15446.
Liao Y, Smyth GK, Shi W (2014) featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics 30:923–30. https://doi.org/10.1093/bioinformatics/btt656
Liu P, Luo L, Guo J, Liu H, Wang B, Deng B, Long CA, Cheng Y (2010) Farnesol induces apoptosis and oxidative stress in the fungal pathogen Penicillium expansum. Mycologia 102:311–318. https://doi.org/10.3852/09-176.
Liu N, Wu S, Dawood DH, Tang G, Zhang C, Liang J, Chen Y, Ma Z (2019) The b-ZIP transcription factor FgTfmI is required for the fungicide phenamacril tolerance and pathogenecity in Fusarium graminearum. Pest Manag Sci 75:3312–3322. https://doi.org/10.1002/ps.5454.
Lou Y, Zhang J, Wang G, Fang W, Wang S, Abubakar YS, Zhou J, Wang Z, Zheng W (2021) Genome-wide characterization of PX domain-containing proteins involved in membrane trafficking-dependent growth and pathogenicity of Fusarium graminearum. mBio 12:e0232421. https://doi.org/10.1128/mBio.02324-21.
Lu P, Chen D, Qi Z, Wang H, Chen Y, Wang Q, Jiang C, Xu JR, Liu H (2022) Landscape and regulation of alternative splicing and alternative polyadenylation in a plant pathogenic fungus. New Phytol 235:674–689. https://doi.org/10.1111/nph.18164.
Luo K, Rocheleau H, Qi PF, Zheng YL, Zhao HY, Ouellet T (2016) Indole-3-acetic acid in Fusarium graminearum: Identification of biosynthetic pathways and characterization of physiological effects. Fungal Biol 120:1135–1145. https://doi.org/10.1016/j.funbio.2016.06.002.
Luo K, DesRoches CL, Johnston A, Harris LJ, Zhao HY, Ouellet T (2017) Multiple metabolic pathways for metabolism of ltryptophan in Fusarium graminearum. Can J Microbiol 63:921–927. https://doi.org/10.1139/cjm-2017-0383.
Manzo-Valencia MK, Valdés-Santiago L, Sánchez-Segura L, Guzmán-de-Peña DL (2016) Naphthalene acetic acid potassium salt (NAA-K( )) affects conidial germination, sporulation, mycelial growth, cell surface morphology, and viability of Fusarium oxysporum f. sp. radici-lycopersici and F. oxysporum f. sp. cubense in vitro. J Agric Food Chem 64:8315–8323. https://doi.org/10.1021/acs.jafc.6b03105.
Mao X, Wu Z, Bi C, Wang J, Zhao F, Gao J, Hou Y, Zhou M (2020) Molecular and biochemical characterization of pydiflumetofen-resistant mutants of Didymella bryoniae. J Agric Food Chem 68:9120–9130. https://doi.org/10.1021/acs.jafc.0c03690.
Menke J, Dong Y, Kistler HC (2012) Fusarium graminearum Tri12p influences virulence to wheat and trichothecene accumulation. Mol Plant Microbe Interact 25:1408–1418. https://doi.org/10.1094/MPMI-04-12-0081-R
Menke J, Weber J, Broz K, Kistler HC (2013) Cellular development associated with induced mycotoxin synthesis in the filamentous fungus Fusarium graminearum. PLoS One 8:e63077. https://doi.org/10.1371/journal.pone.0063077.
Nicastro R, Raucci S, Michel AH, Stumpe M, Garcia Osuna GM, Jaquenoud M, Kornmann B, De Virgilio C (2021) Indole-3-acetic acid is a physiological inhibitor of TORC1 in yeast. PLoS Genet 17:e1009414. https://doi.org/10.1371/journal.pgen.1009414.
Nykiel-Szymańska J, Stolarek P, Bernat P (2018) Elimination and detoxification of 2,4-D by Umbelopsis isabellina with the involvement of cytochrome P450. Environ Sci Pollut Res Int 25:2738–2743. https://doi.org/10.1007/s11356-017-0571-4
Oparka M, Walczak J, Malinska D, van Oppen LMPE, Szczepanowska J, Koopman WJH, Wieckowski MR (2016) Quantifying ROS levels using CM-H(2)DCFDA and HyPer. Methods 109:3–11. https://doi.org/10.1016/j.ymeth.2016.06.008.
Petti C, Reiber K, Ali SS, Berney M, Doohan FM (2012) Auxin as a player in the biocontrol of Fusarium head blight disease of barley and its potential as a disease control agent. BMC Plant Biol 12:224. https://doi.org/10.1186/1471-2229-12-224.
Proctor RH, Hohn TM, McCormick SP (1995) Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Mol Plant Microbe Interact 8:593–601. https://doi.org/10.1094/mpmi-8-0593
Proctor RH, Hohn TM, McCormick SP, Desjardins AE (1995) Tri6 encodes an unusual zinc finger protein involved in regulation of trichothecene biosynthesis in Fusarium sporotrichioides. Appl Environ Microbiol 61:1923–1930. https://doi.org/10.1128/aem.61.5.1923-1930.1995.
Reineke G, Heinze B, Schirawski J, Buettner H, Kahmann R, Basse CW (2008) Indole-3-acetic acid (IAA) biosynthesis in the smut fungus Ustilago maydis and its relevance for increased IAA levels in infected tissue and host tumour formation. Mol Plant Pathol 9:339–355. https://doi.org/10.1111/j.1364-3703.2008.00470.x.
Ren J, Li C, Gao C, Xu JR, Jiang C, Wang G (2019) Deletion of FgHOG1 Is suppressive to the mgv1 mutant by stimulating Gpmk1 activation and avoiding intracellular turgor elevation in Fusarium graminearum. Front Microbiol 10:1073. https://doi.org/10.3389/fmicb.2019.01073.
Ren J, Zhang Y, Wang Y, Li C, Bian Z, Zhang X, Liu H, Xu JR, Jiang C (2022) Deletion of all three MAP kinase genes results in severe defects in stress responses and pathogenesis in Fusarium graminearum. Stress Biol 2:6. https://doi.org/10.1007/s44154-021-00025-y.
Romero-Puertas MC, Peláez-Vico M, Pazmiño DM, Rodríguez-Serrano M, Terrón-Camero L, Bautista R, Gómez-Cadenas A, Claros MG, León J, Sandalio LM (2022) Insights into ROS-dependent signalling underlying transcriptomic plant responses to the herbicide 2,4-D. Plant Cell Environ 45:572–590. https://doi.org/10.1111/pce.14229.
Shin YK, Kim DW, Lee SW, Lee MJ, Gi Baek S, Lee T, Yun SH (2022) Functional roles of all five putative hydrophobin genes in growth, development, and secondary metabolism in Fusarium graminearum. Fungal Genet Biol 160:103683. https://doi.org/10.1016/j.fgb.2022.103683.
Smith JE, Bluhm BH (2011) Metabolic fingerprinting in Fusarium verticillioides to determine gene function. Methods Mol Biol 722:237–247. https://doi.org/10.1007/978-1-61779-040-9_18
Son H, Seo YS, Min K, Park AR, Lee J, Jin JM et al (2011) A phenome-based functional analysis of transcription factors in the cereal head blight fungus. Fusarium graminearum. PLoS Pathog 7:e1002310. https://doi.org/10.1371/journal.ppat.1002310.
Spicer R, Salek RM, Moreno P, Cañueto D, Steinbeck C (2017) Navigating freely-available software tools for metabolomics analysis. Metabolomics 13:106. https://doi.org/10.1007/s11306-017-1242-7.
Sun S, Shen Y, Wang J, Li J, Cao J, Zhang J (2021) Identification and validation of autophagy-related genes in chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis 16:67–78. https://doi.org/10.2147/copd.s288428.
Svoboda T, Thon MR, Strauss J (2021) The role of plant hormones in the interaction of colletotrichum species with their host plants. Int J Mol Sci 22:12454. https://doi.org/10.3390/ijms222212454
Takeda N, Handa Y, Tsuzuki S, Kojima M, Sakakibara H, Kawaguchi M (2015) Gibberellins interfere with symbiosis signaling and gene expression and alter colonization by arbuscular mycorrhizal fungi in Lotus japonicus. Plant Physiol 167:545–557. https://doi.org/10.1104/pp.114.247700.
Tatebayashi K, Yamamoto K, Tomida T, Nishimura A, Takayama T, Oyama M, Kozuka-Hata H, Adachi-Akahane S, Tokunaga Y, Saito H (2020) Osmostress enhances activating phosphorylation of Hog1 MAP kinase by mono-phosphorylated Pbs2 MAP2K. Embo j 39:e103444. https://doi.org/10.15252/embj.2019103444.
Van Thuat N, Schäfer W, Bormann J (2012) The stress-activated protein kinase FgOS-2 is a key regulator in the life cycle of the cereal pathogen Fusarium graminearum. Mol Plant Microbe Interact 25:1142–1156. https://doi.org/10.1094/mpmi-02-12-0047-r
Wang C, Zhang S, Hou R, Zhao Z, Zheng Q, Xu Q et al (2011) Functional analysis of the kinome of the wheat scab fungus Fusarium graminearum. PLoS Pathog 7:e1002460. https://doi.org/10.1371/journal.ppat.1002460.
Wang L, Mogg C, Walkowiak S, Joshi M, Subramaniam R (2014) Characterization of NADPH oxidase genes NoxA and NoxB in Fusarium graminearum. Can J Plant Pathol 36:12–21. https://doi.org/10.1080/07060661.2013.868370.
Wang Q, Chen D, Wu M, Zhu J, Jiang C, Xu JR, Liu H (2018) MFS transporters and GABA metabolism are involved in the self-defense against DON in Fusarium graminearum. Front Plant Sci 9:438. https://doi.org/10.3389/fpls.2018.00438.
Wang H, Chen D, Li C, Tian N, Zhang J, Xu JR, Wang C (2019) Stage-specific functional relationships between Tub1 and Tub2 beta-tubulins in the wheat scab fungus Fusarium graminearum. Fungal Genet Biol 132:103251. https://doi.org/10.1016/j.fgb.2019.103251.
Wen Z, Wang J, Jiao C, Shao W, Ma Z (2022) Biological and molecular characterizations of field fludioxonil-resistant isolates of Fusarium graminearum. Pestic Biochem Physiol 184:105101. https://doi.org/10.1016/j.pestbp.2022.105101.
Wójcik AM, Wójcikowska B, Gaj MD (2020) Current perspectives on the auxin-mediated genetic network that controls the induction of somatic embryogenesis in plants. Int J Biochem Cell Biol 21:1333. https://doi.org/10.3390/ijms21041333
Wu AB, Li HP, Zhao CS, Liao YC (2005) Comparative pathogenicity of Fusarium graminearum isolates from China revealed by wheat coleoptile and floret inoculations. Mycopathologia 160:75–83. https://doi.org/10.1007/s11046-005-1153-4.
Xu JR (2000) Map kinases in fungal pathogens. Fungal Genet Biol 31:137–152. https://doi.org/10.1006/fgbi.2000.1237
Yin J, Hao C, Niu G, Wang W, Wang G, Xiang P, Xu JR, Zhang X (2020) FgPal1 regulates morphogenesis and pathogenesis in Fusarium graminearum. Environ Microbiol 22:5373–5386. https://doi.org/10.1111/1462-2920.15266.
Yu HY, Seo JA, Kim JE, Han KH, Shim WB, Yun SH, Lee YW (2008) Functional analyses of heterotrimeric G protein G alpha and G beta subunits in Gibberella zeae. Microbiology (Reading) 154:392–401. https://doi.org/10.1099/mic.0.2007/012260-0.
Zhang Y, Lamm R, Pillonel C, Lam S, Xu JR (2002) Osmoregulation and fungicide resistance: the Neurospora crassa os-2 gene encodes a HOG1 mitogen-activated protein kinase homologue. Environ Microbiol 68:532–538. https://doi.org/10.1128/aem.68.2.532-538.2002.
Zhang C, Wang Y, Wang J, Zhai Z, Zhang L, Zheng W et al (2013) Functional characterization of Rho family small GTPases in Fusarium graminearum. Fungal Genet Biol 61:90–99. https://doi.org/10.1016/j.fgb.2013.09.001.
Zhang X, Liu W, Li Y, Li G, Xu JR (2017) Expression of HopAI interferes with MAP kinase signalling in Magnaporthe oryzae. Environ Microbiol 19:4190–4204. https://doi.org/10.1111/1462-2920.13884.
Zhang X, Bian Z, Xu JR (2018) Assays for MAP kinase activation in Magnaporthe oryzae and other plant pathogenic fungi. Methods Mol Biol 1848:93–101. https://doi.org/10.1007/978-1-4939-8724-5_8
Zhang X, Wang Z, Jiang C, Xu JR (2021) Regulation of biotic interactions and responses to abiotic stresses by MAP kinase pathways in plant pathogenic fungi. Stress Biol 1:5. https://doi.org/10.1007/s44154-021-00004-3.
Zheng D, Zhang S, Zhou X, Wang C, Xiang P, Zheng Q, Xu JR (2012) The FgHOG1 pathway regulates hyphal growth, stress responses, and plant infection in Fusarium graminearum. PLoS One 7:e49495. https://doi.org/10.1371/journal.pone.0049495.
Zhou X, Heyer C, Choi YE, Mehrabi R, Xu JR (2010) The CID1 cyclin C-like gene is important for plant infection in Fusarium graminearum. Fungal Genet Biol 47:143–151. https://doi.org/10.1016/j.fgb.2009.11.001.
Acknowledgements
We thank Drs. Huiquan Liu and Qinhu Wang for fruitful discussions. We thank Drs. Hua Zhao, Fengping Yuan, Guoyun Zhang and Ping Xiang for assistance with microscopic examinations and metabolites detection.
Funding
This work was supported by grant to Ping Xiang from Shaanxi Provincial Department of Science and Technology (No.2023-JC-QN-0177), grant to Xue Zhang from National Natural Science Foundation of China (No. 3210170916), and grants to Jin-Rong Xu from NSF and USWBSI.
Author information
Authors and Affiliations
Contributions
KD, QS, YW, PX, YS, CY, CJ, and GW conducted the experiments and data analysis. JRX and XZ designed the experiments and wrote this manuscript.
Corresponding authors
Ethics declarations
Ethics approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Competing interests
JRX is a member of the Editorial Board, but was not involved in the journal's review of, or any decisions related to, this manuscript.
Additional information
Handling Editor: Zu-Hua He.
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Additional file 1: Table S1.
Metabolic profiles of PH-1, the Fghog1 mutant HG6, and FgPBS2S451D T455D transformant Pbs2-DA.
Additional file 2: Table S2.
Expression profiles of PH-1 treated with 0.4 % ethanol or 500 µM 2,4-D treatment.
Additional file 3: Table S3.
Primers used in this study.
Additional file 4: Fig. S1.
The effects of different concentrations of 2,4-D on FgHog1 activation. Western blots of total proteins isolated from vegetative hyphae of PH-1 treated with marked concentrations of 2,4-D for 30 min were detected with the anti-TpGY phosphorylation-specific and anti-FgHog1 antibodies. Fig. S2. KEGG enrichment analysis of genes affected by 2,4-D. KEGG enrichment analysis of the differentially expressed genes (DEGs) up-regulated (A) and down-regulated (B) in cultures treated with 500 µM 2,4-D. The numbers in brackets are the number of DEGs in each category.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Duan, K., Shen, Q., Wang, Y. et al. Herbicide 2,4-dichlorophenoxyacetic acid interferes with MAP kinase signaling in Fusarium graminearum and is inhibitory to fungal growth and pathogenesis. Stress Biology 3, 31 (2023). https://doi.org/10.1007/s44154-023-00109-x
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s44154-023-00109-x