Abstract
Trembleya phlogiformis DC, Melastomataceae, is a shrub whose leaves are used as a dye for dyeing wool and cotton. The present article aimed to carry out the morphological description of the species, to study the chemical composition of volatile oils from the leaves and flowers and the seasonal variability from the leaves during a year. Macroscopic characterization was carried out with the naked eye and with a stereoscopic microscope. Volatile oils were isolated by hydrodistillation in Clevenger apparatus and analyzed by gas chromatography/mass spectrometry. The major components of the volatile oil of T. phlogiformis flowers were: n-heneicosane (33.5%), phytol (12.3%), n-tricosane (8.4%) and linoleic acid (6.1%). It was verified the existence of a large chemical variability of the volatile oils from the leaves of T. phlogiformis over the months, with the majority compound (oleic alcohol, ranging from 5.7 to 26.8%) present in all samples. A combination of Cluster Analysis and Principal Component Analysis showed the existence of three main clusters, probably related to the seasons. The results suggested that the volatile oils of T. phlogiformis leaves possess high chemical variability, probably related to variation associated with rainfall and the variation in the behavior of specimens throughout the year. This research provides insights for future studies on the volatile oils obtained from the T. phlogiformis leaves and flowers, mainly related to biological markers of applications monitored in the leaves and flowers of this species.
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SRF contributed in collecting plant sample, running the laboratory work. HDF contributed in collecting plant sample and identification, confection of herbarium and morphological description of the species. SS contributed in collecting plant sample, running the laboratory work. LLB contributed to the statistical analyzes. LMFT contributed to critical reading of the manuscript. PHF contributed to chromatographic analysis. JRP contributed to chemical studies, chromatographic analysis and critical reading of the manuscript. TSF designed the study, supervised the laboratory work contributed to biological and chemical studies, chromatographic analysis and drafted the paper and contributed to critical reading of the manuscript. All the authors had read the final manuscript and approved the submission contributed to biological studies running the laboratory work, analysis of the data.
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Fernandes, S.R., Ferreira, H.D., de Sá, S. et al. Chemical composition and seasonal variation of the volatile oils from Trembleya phlogiformis leaves. Rev. Bras. Farmacogn. 27, 419–425 (2017). https://doi.org/10.1016/j.bjp.2017.03.003
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DOI: https://doi.org/10.1016/j.bjp.2017.03.003