Abstract
The excessive sun exposure, coupled with lack of sun protection represents one of the biggest risks to the occurrence of skin cancer and photoaging. Recent strategies for photoprotection have included the incorporation of natural antioxidant and anti-inflammatory compounds, into sunscreens, and the oral administration of natural antioxidant extracts. In this work, we use Brazilian red propolis extract because its antioxidant and anti-inflammatory activities. The aim of this work was to evaluate the sun protection factor and antioxidant activity of different hydroalcoholic extracts of red propolis (70% and 75%) prepared from lyophilized red propolis at room and high temperatures. The sun protection factor in vitro was determined by a spectrophotometric method developed by Mansur. The hydroalcoholic extracts of red propolis incorporated with Filter UVA-UVB 5% Gel Permulem TR-1 presented absorption in the UVB region. Also, the in vitro capacity of the hydroalcoholic extracts of red propolis to increase photoprotective activity of Filter UVA-UVB 5% Gel was evaluated. The hydroalcoholic extracts of red propolis incorporated presented higher values of sun protection factor and showed synergism in the photoprotective activity of Filter UVA-UVB 5% Gel Permulem TR-1. The antioxidant activity and sun protection factor are correlated with total phenolics content of the extracts and the hydroalcoholic extract of red propolis 75% at room temperature was choosen. The formulation developed with Filter UVA-UVB 5% Gel Permulem TR-1 with this hydroalcoholic extract of red propolis showed safe to be applied on the skin according HET-CAM test. Suggests indication of hydroalcoholic extract of red propolis (75% - room temperature) associated to photoprotective formulations for use in photoprotective products.
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WASA (undergraduate studentt) contributed incorporation of red propolis extracts in the filter, contributed Sun Protection Factor studies, running the laboratory work. AAS (undergraduate student) contributed evaluation of photostability. RGP (undergraduate student) contributed Sun Protection Factor studies. HSGC (undergraduate student) contributed preparation of ethanolic extracts of red propolis. AMN contributed chemical studies, supervised the laboratory work and critical reading of the manuscript. ALA designed the study and contributed to critical reading of the manuscript. VRS contributed in collecting red propolis sample and identification. TC contributed Sun Protection Factor studies, contributed photostabilit studies and designed the graphs. TRA (post grad student) contributed to evaluate the antioxidant activity, contributed determination of total phenolic and flavonoid content and Statistical analysis. GHBS supervised the laboratory work, contributed Biological studies and contributed to critical reading of the manuscript. KMF (post-doc fellow) contributed HETCAM test. ODHS supervised the laboratory work, contributed biological studies and contributed HETCAM studies. LRS contributed antioxidant activity studies. VMRS designed the study, supervised the experiments, wrote the manuscript and contributed to critical, reading of the manuscript. All the authors have read the final manuscript,approved the submission and contributed biological studies and chemical studies. All the authors have real contribution to the final manuscript writing.
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da Almeida, W.A.S., dos Santos Antunes, A., Penido, R.G. et al. Photoprotective activity and increase of SPF in sunscreen formulation using lyophilized red propolis extracts from Alagoas. Rev. Bras. Farmacogn. 29, 373–380 (2019). https://doi.org/10.1016/j.bjp.2019.02.003
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DOI: https://doi.org/10.1016/j.bjp.2019.02.003