Abstract
Intracellular retrograde transport in eukaryotic cells relies exclusively on the molecular motor cytoplasmic dynein 1. Unlike its counterpart, kinesin, dynein has a single isoform, which raises questions about its cargo specificity and regulatory mechanisms. The precision of dynein-mediated cargo transport is governed by a multitude of factors, including temperature, phosphorylation, the microtubule track, and interactions with a family of activating adaptor proteins. Activating adaptors are of particular importance because they not only activate the unidirectional motility of the motor but also connect a diverse array of cargoes with the dynein motor. Therefore, it is unsurprising that dysregulation of the dynein-activating adaptor transport machinery can lead to diseases such as spinal muscular atrophy, lower extremity, and dominant. Here, we discuss dynein motor motility within cells and in in vitro, and we present several methodologies employed to track the motion of the motor. We highlight several newly identified activating adaptors and their roles in regulating dynein. Finally, we explore the potential therapeutic applications of manipulating dynein transport to address diseases linked to dynein malfunction.
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Introduction
Within the cytoplasm of eukaryotic cells, various functionally necessary cellular components undergo continuous movement from one location to another. Long-range transport is accomplished by the movement of molecular motors along the microtubule (MT) track, while myosin movement along actin filaments provides short-range transport1,2,3. Cytoplasmic dynein 1 (hereafter referred to as dynein) is responsible for transporting a wide array of diverse cargoes along the MT track and performing numerous functions within eukaryotic cells4,5. In contrast to microtubule-based anterograde motor kinesin transport cargoes, which exhibit diverse isoforms for cargo binding, dynein has a single isoform6,7. This observation raises questions regarding how cargo specificity is achieved and how regulatory mechanisms govern dynein-mediated intracellular transport8. Elucidating the mechanisms that govern the cargo specificity of dynein has been a longstanding focus of research on dynein function. Nonetheless, due to the immense complexity and dynamic nature of the molecule, systematic investigations of the dynein motor in cellular contexts as well as in noncellular models in vitro have been very challenging, making it difficult to achieve a comprehensive understanding of the molecular mechanism of the motor9,10. Owing to recent advances in structural, biophysical, and cellular approaches, we are now gaining insight into the intricate processes by which dynein facilitates the intracellular transport of various cargoes.
Given the complexity of the dynein molecule and its involvement in critical cellular functions, of the involvement of a variety of regulatory factors in dynein-mediated cargo transport is no surprise4,5,11. The cargo specificity, activity and force of the dynein motor depend on multiple intramolecular and intermolecular factors, including temperature12, phosphorylation13,14, the MT track15,16,17, and “activating adaptor” binding11. In particular, the family of dynein activating adaptors, of which new members continue to be discovered, is among the major regulators governing the cargo specificity of the dynein motor as well as its force and velocity11,18,19. An activating adaptor not only binds directly to the dynein–dynactin complex and stabilizes it in the active conformation but also links various cargoes to the motor and activates the motility of the motor. Considering the importance of activating adaptors in dynein regulation, mutations affecting the functionality of an activating adaptor or perturbing interactions between dynein and an activating adaptor can result in malfunctions of the dynein complex20,21. Consequently, these mutations are associated with a range of human diseases, notably neurological pathologies such as spinal muscular atrophy, Charcot–Marie–Tooth disease, cortical development malformations, and neurodegenerative diseases22,23,24. Here, we discuss the motility of the motor dynein motor in cells and in noncellular models in vitro and explore the methodologies employed to track the movement of dynein in both contexts. We then describe several recently identified activating adaptors and explore the potential of leveraging the dynein transport machinery for therapeutic applications.
Cytoplasmic dynein 1 transport machinery
Two types of cytoplasmic dynein are responsible for the retrograde transport of cargoes along the MT track within the cytoplasm (cytoplasmic dynein 1) and within cilia and flagella (cytoplasmic dynein 2; intraflagellar transport [IFT] dynein)7,25. Here, we focus on cytoplasmic dynein 1 and refer to it as “dynein”. Human dynein consists of six distinct polypeptide chains, namely, the heavy chain (HC), the intermediate chain (IC), the light intermediate chain (LIC), and three types of light chains (roadblock [Robl], LC8, and Tctex)25 (Fig. 1a). Each of these constituents is present in duplicate, culminating in the formation of a complex with an approximate molecular mass of approximately 1.4 MDa25. By itself, mammalian dynein lacks the capability to selectively engage with particular cargoes and is incapable of processive movement26,27. To undergo processive movement along the MT track in coordination with specific cargo, mammalian dynein forms a complex with two additional components11,28,29: (i) the general adaptor dynactin and (ii) a family of coiled-coil proteins known as “activating adaptors” that recruit specific cargoes to the dynein complex, facilitating the processive motility of the complex. The assembly of the ~3.5 MDa dynein–dynactin-adaptor (DDA) complex introduces a considerable level of complexity and heterogeneity. Therefore, systematically investigating the function, structure, interactions, and molecular mechanisms of this motor both within cells and in noncellular models in vitro is very challenging, often necessitating specialized techniques, as discussed below.
Tracking dynein-driven motility in living cells
The principal techniques for visualizing dynein complexes within cells involve the direct labeling of dynein subunits using synthetic fluorophores or recombinant fluorescent proteins such as green fluorescent protein (GFP)30,31,32,33,34,35 (Table 1). Although directly labeling dynein with a fluorescent marker allows the observation of dynein motility and behavior within a cell, the direct high-resolution visualization of dynein movement in living cells often remains challenging. Many cargoes are attached to other motors and exhibit bidirectional movement along the MT track, precluding the direct determination of dynein-driven motility. For example, in living Dictyostelium discoideum cells, dynein moves at a speed of ~1.8 µm/s toward the minus end of the MT and ~1.7 µm/s toward the plus end of the MT30, implying that both the minus-end-directed motor (e.g., dynein) and the plus-end-directed motor (e.g., kinesins) are simultaneously associated with a certain cargo and that a regulatory mechanism exists to coordinate the activity of the bound motors to control the direction and velocity of cargo transport. Consistent with this, in mammalian cells, GFP-labeled dynein moves along the MT track in both plus end- and minus end-directed motion with a velocity of 1–2 µm/s34. Furthermore, as dynein is ubiquitously expressed at high levels throughout the cytoplasm of many cells, it is often challenging to distinguish dynein moving along the MT track from the diffuse background of dynein in the cytoplasm.
These challenges can at least be partly overcome by imaging and tracking dynein movement using advanced superresolution microscopy techniques, such as highly inclined and laminated optical sheet (HILO) and minimal photon flux (MINFLUX) microscopy, which can enable the observation of more detailed features of dynein movement. HILO microscopy generates a thin excitation plane of several microns36, increasing the signal/background ratio and decreasing photobleaching compared with conventional confocal microscopy because of the nonfocused illumination, thereby providing better spatial and temporal resolution and enabling near-single-molecule tracking of the motor protein inside living cells37. When the movement of mouse dynein heavy chain labeled with GFP in HeLa cells was tracked via HILO microscopy, approximately 30% of the dynein bound to MTs showed processive movements toward the minus end of the MT over a relatively short period (~0.5 s), and the average length was less than one micron38. This finding implies that when traveling distances greater than one micron, dynein–dynactin–cargo adaptor complexes consistently undergo binding and unbinding. While overexpressed GFP–dynein shows a short residence time and short run length inside the cytoplasm of HeLa cells, dynein shows much more robust movement and a longer run length along the axons of human neuronal cells39. Endogenously labeled dynein had a much longer run length of up to ~110 μm and was able to move the entire length of the axon, with an average speed of ~1.7 μm/s, as observed via HILO microscopy. These observations suggest that dynein-driven transport can differ according to cell type and cellular environment, including factors such as the arrangement and dynamics of MT tracks39. Intriguingly, an additional superresolution technique known as MINFLUX microscopy has demonstrated the ability to achieve very high localization precision of a fluorophore, detecting as few as ~20 photons40,41. This technique has been successfully utilized to track the movement of kinesins with high spatial and temporal resolution in cells. Utilizing MINFLUX, the movement of kinesin motors in live cells can be pinpointed with sub-millisecond temporal resolution and a spatial resolution of approximately one nanometer42,43. While this approach has been applied to investigate kinesin behavior within living cells, it has yet to be extended to the study of dynein.
Instead of directly observation of the movement of fluorescently labeled dynein in cells, the motion of dynein can be assessed by tethering the complex to nonnative, static cargoes and subsequently measuring the movement of the cargo instead of that of the motor complex44,45 (Table 2). Inducible cargo trafficking assays artificially recruit the motor complex to a specific cellular compartment (e.g., stationary vesicles such as peroxisomes) using a heterodimerization system such as FKBP-rapalog-FRB34,46,47. The heterodimerization of FKBP and the FKBP–rapamycin binding domain (FRB) is triggered by the addition of a cell-permeable, non-immunosuppressive analog of rapamycin called rapalog. Additionally, a photoactivation system that uses light to achieve noninvasive and high spatiotemporal resolution has emerged as an important system for studying dynein motility in cells34,45,48,49. In this case, the cTMP-Halo tag (cTMP-Htag), which consists of a Halo-tag ligand linked to a photocaged trimethoprim (TMP), is used as a dimerizer. This molecule enables the heterodimerization of Halo tag proteins (Halo) and Escherichia coli (E. coli) dihydrofolate reductase (eDHFR) upon the cleavage of photocaged cTMP–Htag by 405-nm light. When the motor protein is tagged with eDHFR and model cargo vesicles (such as peroxisomes) are tagged with Halo, 405-nm light can induce motor-specific vesicle movement48,49 (Table 2).
Compared to direct dynein subunit labeling strategies, inducible cargo trafficking assays enable the observation of motor-specific translocation events in a more controlled spatiotemporal context. In inducible cargo trafficking assays, dynein-driven motility can be examined by live-cell imaging via high-resolution total internal reflection fluorescence (TIRF) microscopy to analyze particle distribution and velocity. Compared to the bidirectional and stochastic movement of subunit-labeled dynein, vesicles recruited to dynein through the heterodimerization system exhibited unidirectional motility along the MT track, with a mean velocity of ~1 µm/s.
Tracking dynein-driven motility in vitro
By reconstituting the dynein–dynactin complex and adaptor protein, the motility of the dynein complex can be directly observed in a much more controlled context, excluding many cellular environmental factors that affect the motility of the motor. The greatest pitfall of this system is the purification of extremely complex, multisubunit components (i.e., dynein, dynactin, and the activating adaptor) and the reconstitution of the active massive tripartite motor. Each component can be purified separately and then reconstituted50,51 or can be purified from brain tissue or mammalian cells using a purified activating adaptor as bait28,52. Otherwise, cellular lysates containing overexpressed fluorescently labeled adaptors can be directly used for imaging without a purification step49, although this method cannot reliably exclude other factors (e.g., other adaptor complexes) that might affect the motility of the motor. The motility of the motor can then be observed using total internal reflection microscopy.
Dynein-activating adaptors
The motility and function of mammalian dynein depend on a wide variety of factors, with one critical regulatory factor being its interaction with the activating adaptor family11. Dynein forms a tripartite complex with its general adaptor dynactin and the activating adaptor, and this complex can achieve robust motility on MT tracks18,53,54,55,56. The term “activating adaptor” is more specific than the general terms “adaptor” and “cargo adaptor”. This distinction arises from experimental evidence that activating adaptors not only link cargo to dynein-like adaptors but also enhance the processive motion of the dynein motor11. Since the discovery of activating adaptors that facilitate the motility of the dynein complex, extensive research has been conducted on various activating adaptor families, revealing their roles in regulating motor velocity, cargo recognition, and force generation. The activating adaptor family generally performs dual functions: (i) releasing the autoinhibited conformation (and stabilizing the activated conformation) of dynein through interaction with its N-terminal LIC-binding domain and the very long (~200–300 amino acids) central coiled-coil domain that can run along the whole dynactin filament and ii) linking the specific cargo to the dynein–dynactin complex through its cargo-binding domain at the C-terminus11. Although the activating adaptor families share no sequence homology, these common features enable the categorization of those families as activating adaptors. Different activating adaptors have different effects on the activation57 of the dynein complex. This variation is evident in the differences in both velocity and run length within the dynein–dynactin–adaptor (DDA) complex, which differ depending on the specific type of activating adaptor that is attached.
At the time of writing, more than a dozen activating adaptors (e.g., Hook1, Hook3, BICD2, BICDR1, Spindly, NIN (Ninein), NINL (Ninein-like), CRACR2a, Rab45, Rab11-FIP3 (FIP3), KASH5, TRAK1, TRAK2, and JIP3) have been identified28,49,57,58,59,60,61,62,63,64,65,66,67,68,69. Given the existence of comprehensive review papers on activating adaptors11,70, we offer a concise overview of several recently identified activating adaptors as well as potential candidates in the following section (Fig. 1b and Table 3).
KASH5
Dynein plays a crucial role during cell division71. Its critical functions include chromosome movement and segregation, centrosome maturation and separation, and proper positioning and maturation of the mitotic spindle72,73. To achieve chromosome movement during cell division before the nuclear envelope breaks down, dynein tethers to the chromosome by interacting with the linker of the nucleo- and cytoskeleton (LINC) complex located in the nuclear envelope74. The LINC complex connects the cytoskeleton, including microtubules, with the nucleus, transmitting the forces generated by the dynein complex to the chromosome74.
The LINC complex spans the double membrane of the nucleus. The core components of the LINC complex are the highly conserved Sad1/UNC-84 (SUN) protein, which spans the inner nuclear membrane, and the Klarsicht/ANC-1/SYNE homology (KASH) protein75. The KASH protein typically comprises spectrin repeats or coiled-coil domains, which extent from the outer nuclear membrane to the cytoplasm, and a single transmembrane domain followed by an ~30 aa KASH domain that interacts with the SUN protein in the space between the outer and inner nuclear membranes76.
During prophase I of meiosis, KASH5, a meiosis-specific isoform of KASH, interacts with SUN1 in the perinuclear space, forming a prophase I-specific LINC complex that links dynein to the chromosomes inside the nuclear membrane77. KASH5 immunoprecipitates with dynein and shares common structural features with activating adaptors, such as N-terminal EF-hands followed by coiled coils comprising ~200 amino acids77. This observation led to the hypothesis that KASH5 functions as an activating adaptor. Recent studies have provided experimental evidence supporting this idea, demonstrating that KASH5 binds directly to the LIC subunit of dynein, forms a complex with dynein–dynactin, and thereby activates its motility57,62. These observations confirmed KASH5 as the first known activating adaptor containing a transmembrane domain. Given that other KASH proteins also contain LIC binding domains at the N-terminus followed by long coiled coils, it would be intriguing to explore whether other KASH isoforms also serve as activating adaptors78.
Kazrin C
Kazrin is an evolutionarily conserved cytoplasmic protein that is widely expressed in vertebrates79. However, it does not exhibit significant sequence homology with other known proteins, and its exact function has not been fully elucidated. Kazrin has been reported to have various functions, including desmosome assembly, cell adhesion, cytoskeleton organization, and epidermal differentiation79,80. In particular, among the seven isoforms (A-F and K), Kazrin C is specifically involved in early endosome (EE) trafficking, as it attaches directly to several EE components through its C-terminal intrinsically disordered region (IDR)67. Knocking out Kazrin C or inhibiting dynein disrupts the colocalization of juxtanuclear localization EEs and Kazrin C, suggesting that Kazrin C might be involved in dynein-mediated retrograde transport. The domain architecture of Kazrin C is also similar to that of other dynein-activating adaptors, as it contains an N-terminal globular domain, followed by long coiled coils and C-terminal IDRs that interact with vesicular compartments. Furthermore, Kazrin C interacts directly with the LIC1 subunit of dynein, further suggesting that Kazrin C might be a new member of the family of dynein-activating adaptors67. Although Kazrin C has not been directly tested for the ability to function as an activating adaptor (i.e., single-molecule motility assay using purified components), it would be intriguing to determine its role in dynein-mediated endosomal trafficking.
RUFY1
RUFY proteins are a family of cytosolic proteins that contain an N-terminal RUN domain (named after the proteins RPIP8, UNC-14 and NESCA), a C-terminal FYVE domain (named after the proteins Fab1, YOTB/ZK632.12, Vac1, and EEA1), and coiled-coil domains. The RUN and FYVE domains define the characteristic molecular features of the RUFY protein family, which comprises four members in mammals: RUFY1, RUFY2, RUFY3, and RUFY481. The FYVE domain specifically binds to the membrane-embedded phosphatidylinositol 3-phosphate (PI3P) through its zinc finger domain and consecutively targets the RUFY proteins toward the endosomal membrane82. The RUN domain at the N-terminus has been shown to interact with diverse GTPases83 and is thus involved in GTPase signaling84. Since the domain architecture of the RUFY family resembles that of the dynein activating adaptor family and since RUFYs participate in intracellular cargo trafficking, vesicular transport and fusion, RUFYs have been hypothesized to t function as activating adaptors of dynein.
Recently, RUFY1 was shown to interact directly with Arl8b, an Arf-like small GTPase protein68,85. Arl8b is a member of the ARF (ADP-ribosylation factor) family, which is a subgroup of the small GTPase superfamily. Like other small GTPase proteins, Arl8b is involved in various cellular processes, including intracellular vesicle trafficking, lysosomal function, and endosomal dynamics86. RUFY1 binds directly to Arl8b through its RUN domain at the N-terminus and colocalizes with Arl8b to Rab14-positive recycling/sorting endosomes, suggesting a collaborative role of RUFY1 and Arl8b in orchestrating endosomal trafficking processes68. Notably, proteomic investigation revealed interaction between RUFY1 and the dynein/dynactin subunits. A pull-down analysis further confirmed the direct interaction between the RUN domain of RUFY1 and the LIC1 subunit of the dynein complex, which is one of the common characteristics of dynein-activating adaptors68.
In addition to RUFY1, RUFY3 and RUFY4 have been shown to interact directly with Arl8b85. Compared to RUFY1, which participates in endosomal sorting in cells, RUFY3 and RUFY4 colocalize in lysosomes and participate in lysosomal positioning. Akin to RUFY1, RUFY3, and RUFY4 also immunoprecipitated with dynein/dynactin subunits; furthermore, purified RUFY3 directly interacted with the LIC1 subunit of the dynein complex. Targeting RUFY3 and RUFY4 to the stationary vesicle peroxisome promoted perinuclear clustering in a dynein- and dynactin-dependent manner, further supporting the hypothesis that RUFY3 and RUFY4 might function as dynein-activating adaptors85.
TRAK1/2
The bidirectional transport of mitochondria along microtubules is achieved by dynein and kinesin87. This process involves precise coordination between the mitochondrial adaptor Mitochondrial Rho-like (Miro) and the motor adaptor Trafficking Kinesin protein (TRAK)88. Miro, a Rho GTPase protein embedded in outer membrane of mitochondria via its C-terminal transmembrane domain, interacts with the dynein adaptor TRAK through its N-terminal tandem EF-hand pair ligand mimic (ELM) domain89,90. Both TRAK isoforms 1 and 2 feature a long coiled-coil structure at their N-terminus that recruits the dynein–dynactin complex. Additionally, this region mediates interactions with the opposing motor kinesin64, thereby facilitating the bidirectional movement of mitochondria. Recent single-molecule imaging using purified proteins as well as cell lysates demonstrated that TRAK can activate mammalian dynein–dynactin, confirming its role as a dynein-activating adaptor63,90.
HEATR5B
Due to the lack of sequence homology among cargo adaptor families, identifying cargo adaptors based solely on amino acid sequences is often challenging. Accordingly, a proteomic approach has been employed to identify dynein–dynactin cargo adaptors, leading to the identification of several novel families of activating adaptors60. HEAT repeat-containing protein 5B (HEATR5B) was recently identified as a dynein tail interactor in a proteomic search using the dynein tail domain (comprising dynein heavy chain residues 1-1079, intermediate chain, light intermediate chain, and 3 light chains) as bait69. HEATR5B is a component of the clathrin-coated vesicle (CCV) machinery, suggesting that this protein is involved in vesicle trafficking in the trans-Golgi network (TGN). In the TGN, HEATR5B interacts with adaptor protein complex-1 (AP-1), which coordinates cargo selection and CCV formation. In HeLa cells, HEATR5B comigrated with AP-1-positive structures and promoted the membrane localization and motility of AP-1-positive structures, suggesting that HEATR5B not only acts as a dynein/dynactin binder but also increases the motility of the dynein–dynactin complex69. Although HEATR5B can promote the motility of the dynein–dynactin complex in cells, it seems likely that HEATR5B is not a canonical activating adaptor, as the protein lacks long coiled coils that can run along the dynactin filament and mediate interaction with the dynein tail. Instead, HEATR5B might act as a scaffold for all dynein–dynactin–cargo adaptors, analogous to Ankyrin B91.
Dynein as a drug target
Dynein has been implicated in numerous diseases, particularly those affecting the neurological system92. Given the elongated shape of neurons, the disruption of microtubule-based transport resulting from mutations or dysfunction of components of the dynein motor complex can lead to neuronal degeneration and ultimately to various human neurological disorders92,93,94,95,96. Indeed, mutations in the cytoplasmic dynein 1 heavy chain (DYNC1H1)-encoding gene have been implicated in neurological disorders such as spinal muscular atrophy, lower extremity, dominant (SMA-LED)22, Charcot–Marie–Tooth disease, axonal, type 2 O (CMT2O)23, and malformations of cortical development (MCD)24. Among the array of potentially disease-associated mutations that can be found in DYNC1H1, those correlated with SMA-LED are predominantly localized to the dynein tail domain (Table 4). The tail domain of the dynein heavy chain is responsible for homodimerization as well as interactions with dynactin, other subunits, and activating adaptors25. Interestingly, mutations in genes encoding BICD2, a representative and well-studied cargo adaptor, have also been implicated in SMA-LED97,98,99,100,101,102,103. This observation implies that the relationship between dynein and BICD2 might play an important role in the pathophysiology of SMA-LED.
Spinal muscular atrophy, lower extremity, dominant (SMA-LED) is a very rare subtype of spinal muscular atrophy (SMA), which is a diverse group of human neurodegenerative genetic disorders characterized by the degeneration of spinal motor neurons104,105. Generally, the degeneration of motor neurons leads to skeletal muscle weakness and atrophy (wasting), particularly in the muscles closest to the center of the body, such as those in the back, shoulders, hips, and thighs. SMA encompasses various forms, each of which presents with shared characteristics but a distinct genetic profile, often impacting specific subsets of neurons and muscles106. SMA can generally be categorized by pattern of weakness, severity, progression of symptoms, mode of inheritance, and associated mutations. The most common form of SMA (type I SMA or autosomal recessive proximal SMA) is caused by the loss of the survival motor neuron 1 (SMN1) gene encoding the protein SMN1, which is essential for motor neuron survival106. SMA-LED represents an exceedingly rare subtype of SMA, initially identified in a North American family107. Unlike the majority of SMA types, which are recessively inherited, SMA-LED is inherited in a dominant manner105. SMA-LED patients exhibit prominent quadriceps atrophy and weakness of hip adductors, with normal upper extremity muscle strength and sensation and without cognitive retardation108. SMA-LED can be further divided into SMALED1 and SMALED2, which are caused by heterozygous mutations in the dynein 1 heavy chain (DYNC1H1) and the activating adaptor BICD2, respectively109.
While numerous disease-related mutations have been documented110,111,112,113,114,115,116,117,118,119,120,121, the specific pathological impacts of the DYNC1H1 or BICD2 mutations underlying SMALED1 and SMALED2 have not been determined. In an in vitro single-molecule motility assay, the dynein–dynactin–BICD2 (DDB) complex with SMA-LED1-associated mutations in DYNC1H1 was shown to decrease the number of processive complexes, the run length, the velocity20. In contrast, the disease-associated mutation in BICD2 increases the interaction between dynein and BICD252. Furthermore, compared with wild-type BICD2, the increased binding results in a significantly elevated quantity of motile dynein molecules, suggesting that disease-associated mutations in BICD2 hyperactivate the DDB complex52. In addition to affecting the functionality of the dynein motor transport machinery, disease-associated mutations in BICD2 have other functional consequences that cause pathological defects. For example, overexpression of the BICD2 gene in primary motor neurons has been shown to increase the stability of the MT track, accompanied by axonal aberrations122. Importantly, mutations linked to neuropathy within the kinesin motor affect motor activity, underscoring the essential need for a proper balance of motor function in both the anterograde and retrograde directions for optimal neuronal health123,124,125,126,127.
The role of dynein in human disease has prompted increasing interest in developing small-molecule dynein modulators for investigating the functional mechanisms of dynein and exploring the possibility of mitigating diseases associated with dynein128,129,130,131,132 (Table 5). The characteristics of these small-molecule compounds, including their binding sites and functional consequences, vary greatly. Given the critical role of dynein in many human diseases, modulating disease-specific dynein-associated malfunctions using a small-molecule compound would be of great interest.
Prospects
The intricate nature of the dynein molecule has hampered the comprehensive understanding of the molecular mechanisms governing dynein regulation. Nonetheless, recent advances in structural, biophysical, and superresolution microscopy techniques are enabling the elucidation of how this massive and complex molecule functions in such a diverse range of tasks.
Considering the diversity of the functional properties of dynein, including those related to cargo transport, it is reasonable to speculate that a broader array of adaptor families may exist for the specific purpose of linking diverse cargoes to the dynein molecule, as well as controlling the velocity and force generation of the molecule. Given the central importance of dynein in cellular physiology, especially within neuronal contexts, unraveling the molecular foundations of dynein-mediated cargo transport holds significant therapeutic potential.
References
Huang, J.-D. et al. Direct interaction of microtubule-and actin-based transport motors. Nature 397, 267–270 (1999).
Caviston, J. P. & Holzbaur, E. L. Microtubule motors at the intersection of trafficking and transport. Trends Cell Biol. 16, 530–537 (2006).
Kneussel, M. & Wagner, W. Myosin motors at neuronal synapses: drivers of membrane transport and actin dynamics. Nat. Rev. Neurosci. 14, 233–247 (2013).
Cianfrocco, M. A., DeSantis, M. E., Leschziner, A. E. & Reck-Peterson, S. L. Mechanism and regulation of cytoplasmic dynein. Annu. Rev. Cell Dev. Biol. 31, 83–108 (2015).
Kardon, J. R. & Vale, R. D. Regulators of the cytoplasmic dynein motor. Nat. Rev. Mol. Cell. Biol. 10, 854–865 (2009).
Verhey, K. J. & Hammond, J. W. Traffic control: regulation of kinesin motors. Nat. Rev. Mol. Cell. Biol. 10, 765–777 (2009).
Höök, P. & Vallee, R. B. The dynein family at a glance. J. Cell Sci. 119, 4369–4371 (2006).
Karcher, R. L., Deacon, S. W. & Gelfand, V. I. Motor–cargo interactions: the key to transport specificity. Trends Cell Biol. 12, 21–27 (2002).
Vallee, R. B., McKenney, R. J. & Ori-McKenney, K. M. Multiple modes of cytoplasmic dynein regulation. Nat. Cell. Biol. 14, 224–230 (2012).
Vallee, R. B., Williams, J. C., Varma, D. & Barnhart, L. E. Dynein: an ancient motor protein involved in multiple modes of transport. J. Neurobiol. 58, 189–200 (2004).
Reck-Peterson, S. L., Redwine, W. B., Vale, R. D. & Carter, A. P. The cytoplasmic dynein transport machinery and its many cargoes. Nat. Rev. Mol. Cell. Biol. 19, 382–398 (2018).
Hong, W., Takshak, A., Osunbayo, O., Kunwar, A. & Vershinin, M. The effect of temperature on Microtubule-Based transport by cytoplasmic dynein and Kinesin-1 motors. Biophys. J. 111, 1287–1294 (2016).
Whyte, J. et al. Phosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis. J. Cell Biol. 183, 819–834 (2008).
Dillman, J. 3rd & Pfister, K. K. Differential phosphorylation in vivo of cytoplasmic dynein associated with anterogradely moving organelles. J. Cell Biol. 127, 1671–1681 (1994).
Chaudhary, A. R., Berger, F., Berger, C. L. & Hendricks, A. G. Tau directs intracellular trafficking by regulating the forces exerted by kinesin and dynein teams. Traffic 19, 111–121 (2018).
Nirschl, J. J., Magiera, M. M., Lazarus, J. E., Janke, C. & Holzbaur, E. L. α-Tubulin tyrosination and CLIP-170 phosphorylation regulate the initiation of dynein-driven transport in neurons. Cell Rep. 14, 2637–2652 (2016).
Duellberg, C. et al. Reconstitution of a hierarchical+ TIP interaction network controlling microtubule end tracking of dynein. Nat. Cell. Biol. 16, 804–811 (2014).
Canty, J. T. & Yildiz, A. Activation and regulation of cytoplasmic dynein. Trends Biochem. Sci. 45, 440–453 (2020).
Elshenawy, M. M. et al. Cargo adaptors regulate stepping and force generation of mammalian dynein–dynactin. Nat. Chem. Biol. 15, 1093–1101 (2019).
Hoang, H. T., Schlager, M. A., Carter, A. P. & Bullock, S. L. DYNC1H1 mutations associated with neurological diseases compromise processivity of dynein–dynactin–cargo adaptor complexes. Proc. Natl Acad. Sci. USA 114, E1597–E1606 (2017).
Cui, H. et al. Coiled‐coil registry shifts in the F684I mutant of Bicaudal D result in cargo‐independent activation of dynein motility. Traffic 21, 463–478 (2020).
Harms, M. et al. Mutations in the tail domain of DYNC1H1 cause dominant spinal muscular atrophy. Neurology 78, 1714–1720 (2012).
Weedon, M. N. et al. Exome sequencing identifies a DYNC1H1 mutation in a large pedigree with dominant axonal Charcot-Marie-Tooth disease. Am. J. Hum. Genet. 89, 308–312 (2011).
Poirier, K. et al. Mutations in TUBG1, DYNC1H1, KIF5C and KIF2A cause malformations of cortical development and microcephaly. Nat. Genet. 45, 639–647 (2013).
Canty, J. T., Tan, R., Kusakci, E., Fernandes, J. & Yildiz, A. Structure and mechanics of dynein motors. Annu. Rev. Biophys. 50, 549–574 (2021).
Walter, W. J., Brenner, B. & Steffen, W. Cytoplasmic dynein is not a conventional processive motor. J. Struct. Biol. 170, 266–269 (2010).
Torisawa, T. et al. Autoinhibition and cooperative activation mechanisms of cytoplasmic dynein. Nat. Cell Biol. 16, 1118–1124 (2014).
McKenney, R. J., Huynh, W., Tanenbaum, M. E., Bhabha, G. & Vale, R. D. Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes. Science 345, 337–341 (2014).
Schroer, T. A. Dynactin. Annu. Rev. Cell Dev. Biol. 20, 759–779 (2004).
Ma, S. & Chisholm, R. L. Cytoplasmic dynein-associated structures move bidirectionally in vivo. J. Cell Biol. 115, 1453–1460 (2002).
Presley, J. F. et al. ER-to-Golgi transport visualized in living cells. Nature 389, 81–85 (1997).
Egan, M. J., Tan, K. & Reck-Peterson, S. L. Lis1 is an initiation factor for dynein-driven organelle transport. J. Cell Biol. 197, 971–982 (2012).
Lenz, J.-H., Schuchardt, I., Straube, A. & Steinberg, G. A dynein loading zone for retrograde endosome motility at microtubule plus‐ends. EMBO J. 25, 2275–2286 (2006).
Splinter, D. et al. BICD2, dynactin, and LIS1 cooperate in regulating dynein recruitment to cellular structures. Mol. Biol. Cell 23, 4226–4241 (2012).
Twelvetrees, A. E. et al. The dynamic localization of cytoplasmic dynein in neurons is driven by kinesin-1. Neuron 90, 1000–1015 (2016).
Tokunaga, M., Imamoto, N. & Sakata-Sogawa, K. Highly inclined thin illumination enables clear single-molecule imaging in cells. Nat. Methods 5, 159–161 (2008).
Ananthanarayanan, V. et al. Dynein motion switches from diffusive to directed upon cortical anchoring. Cell 153, 1526–1536 (2013).
Tirumala, N. A. et al. Single-molecule imaging of cytoplasmic dynein in cellulo reveals the mechanism of motor activation and cargo movement. bioRxiv https://doi.org/10.1101/2021.04.05.438428 (2021).
Fellows, A. D., Bruntraeger, M., Burgold, T., Bassett, A. R. & Carter, A. P. Dynein and dynactin move long-range but are delivered separately to the axon tip. bioRxiv https://doi.org/10.1101/2023.07.03.547521 (2023).
Gwosch, K. C. et al. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Nat. Methods 17, 217–224 (2020).
Schmidt, R. et al. MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope. Nat. Commun. 12, 1478 (2021).
Wolff, J. O. et al. MINFLUX dissects the unimpeded walking of kinesin-1. Science 379, 1004–1010 (2023).
Deguchi, T. et al. Direct observation of motor protein stepping in living cells using MINFLUX. Science 379, 1010–1015 (2023).
Kapitein, L. C. et al. Probing intracellular motor protein activity using an inducible cargo trafficking assay. Biophys. J. 99, 2143–2152 (2010).
Ballister, E. R., Ayloo, S., Chenoweth, D. M., Lampson, M. A. & Holzbaur, E. L. Optogenetic control of organelle transport using a photocaged chemical inducer of dimerization. Curr. Biol. 25, R407–R408 (2015).
Efremov, A. K. et al. Delineating cooperative responses of processive motors in living cells. Proc. Natl Acad. Sci. USA 111, E334–E343 (2014).
Rezaul, K. et al. Engineered tug‐of‐war between kinesin and dynein controls direction of microtubule based transport in vivo. Traffic 17, 475–486 (2016).
Ayloo, S., Guedes-Dias, P., Ghiretti, A. E. & Holzbaur, E. L. Dynein efficiently navigates the dendritic cytoskeleton to drive the retrograde trafficking of BDNF/TrkB signaling endosomes. Mol. Biol. Cell 28, 2543–2554 (2017).
Olenick, M. A., Tokito, M., Boczkowska, M., Dominguez, R. & Holzbaur, E. L. Hook adaptors induce unidirectional processive motility by enhancing the dynein-dynactin interaction. J. Biol. Chem. 291, 18239–18251 (2016).
Lau, C. K. Dynein: Methods and Protocols. p. 135-156 (Springer, 2023).
Furuta, A. & Furuta, K. Y. Dynein: Methods and Protocols. p. 157-173 (Springer, 2023).
Huynh, W. & Vale, R. D. Disease-associated mutations in human BICD2 hyperactivate motility of dynein–dynactin. J. Cell Sci. 216, 3051–3060 (2017).
Urnavicius, L. et al. Cryo-EM shows how dynactin recruits two dyneins for faster movement. Nature 554, 202–206 (2018).
Urnavicius, L. et al. The structure of the dynactin complex and its interaction with dynein. Science 347, 1441–1446 (2015).
Chaaban, S. & Carter, A. P. Structure of dynein–dynactin on microtubules shows tandem adaptor binding. Nature 610, 212–216 (2022).
Schlager, M. A., Hoang, H. T., Urnavicius, L., Bullock, S. L. & Carter, A. P. In vitro reconstitution of a highly processive recombinant human dynein complex. EMBO J. 33, 1855–1868 (2014).
Agrawal, R. et al. The KASH5 protein involved in meiotic chromosomal movements is a novel dynein activating adaptor. Elife 11, e78201 (2022).
Schroeder, C. M. & Vale, R. D. Assembly and activation of dynein–dynactin by the cargo adaptor protein Hook3. J. Cell Biol. 214, 309–318 (2016).
Schlager, M. A. et al. Bicaudal d family adaptor proteins control the velocity of Dynein-based movements. Cell Rep. 8, 1248–1256 (2014).
Redwine, W. B. et al. The human cytoplasmic dynein interactome reveals novel activators of motility. eLife 6, e28257 (2017).
Wang, Y. et al. CRACR2a is a calcium-activated dynein adaptor protein that regulates endocytic traffic. J. Cell Biol. 218, 1619–1633 (2019).
Garner, K. E. et al. The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein. J. Cell Biol. 222, e202204042 (2023).
Fenton, A. R., Jongens, T. A. & Holzbaur, E. L. F. Mitochondrial adaptor TRAK2 activates and functionally links opposing kinesin and dynein motors. Nat. Commun. 12, 4578 (2021).
van Spronsen, M. et al. TRAK/Milton motor-adaptor proteins steer mitochondrial trafficking to axons and dendrites. Neuron 77, 485–502 (2013).
Celestino, R. et al. JIP3 interacts with dynein and kinesin-1 to regulate bidirectional organelle transport. J. Cell Biol. 221, e202110057 (2022).
Fu, X. et al. Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking. Elife 11, e82218 (2022).
Hernandez-Perez, I. et al. Kazrin promotes dynein/dynactin-dependent traffic from early to recycling endosomes. Elife 12, e83793 (2023).
Rawat, S. et al. RUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes. J. Cell Biol. 222, e202108001 (2022).
Madan, V. et al. HEATR5B associates with dynein-dynactin and selectively promotes motility of AP1-bound endosomal membranes. bioRxiv https://doi.org/10.1101/2023.03.14.532574 (2023).
Olenick, M. A. & Holzbaur, E. L. Dynein activators and adaptors at a glance. J. Cell Sci. 132, jcs227132 (2019).
Karki, S. & Holzbaur, E. L. Cytoplasmic dynein and dynactin in cell division and intracellular transport. Curr. Opin. Cell Biol. 11, 45–53 (1999).
Roberts, A. J., Kon, T., Knight, P. J., Sutoh, K. & Burgess, S. A. Functions and mechanics of dynein motor proteins. Nat. Rev. Mol. Cell Biol. 14, 713–726 (2013).
Hirokawa, N., Noda, Y. & Okada, Y. Kinesin and dynein superfamily proteins in organelle transport and cell division. Curr. Opin. Cell Biol. 10, 60–73 (1998).
Burke, B. LINC complexes as regulators of meiosis. Curr. Opin. Cell Biol. 52, 22–29 (2018).
Sosa, B. A., Kutay, U. & Schwartz, T. U. Structural insights into LINC complexes. Curr. Opin. Struct. Biol. 23, 285–291 (2013).
Starr, D. A. KASH and SUN proteins. Curr. Biol. 21, R414–R415 (2011).
Morimoto, A. et al. A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. J. Cell Biol. 198, 165–172 (2012).
Kim, D. I., Birendra, K. C. & Roux, K. J. Making the LINC: SUN and KASH protein interactions. Biol. Chem. 396, 295–310 (2015).
Groot, K. R., Sevilla, L. M., Nishi, K., DiColandrea, T. & Watt, F. M. Kazrin, a novel periplakin-interacting protein associated with desmosomes and the keratinocyte plasma membrane. J. Cell Biol. 166, 653–659 (2004).
Sevilla, L. M., Nachat, R., Groot, K. R. & Watt, F. M. Kazrin regulates keratinocyte cytoskeletal networks, intercellular junctions and differentiation. J. Cell Sci. 121, 3561–3569 (2008).
Char, R. & Pierre, P. The RUFYs, a family of effector proteins involved in intracellular trafficking and cytoskeleton dynamics. Front. Cell Dev. Biol. 8, 779 (2020).
Stenmark, H., Aasland, R. & Driscoll, P. C. The phosphatidylinositol 3-phosphate-binding FYVE finger. FEBS Lett. 513, 77–84 (2002).
Rosa-Ferreira, C. & Munro, S. Arl8 and SKIP act together to link lysosomes to kinesin-1. Dev. Cell 21, 1171–1178 (2011).
Callebaut, I., Goud, B. & Mornon, J.-P. RUN domains: a new family of domains involved in Ras-like GTPase signaling. Trends Biochem. Sci. 26, 79–83 (2001).
Keren-Kaplan, T. et al. RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin. Nat. Commun. 13, 1506 (2022).
Garg, S. et al. Lysosomal trafficking, antigen presentation, and microbial killing are controlled by the Arf-like GTPase Arl8b. Immunity 35, 182–193 (2011).
Pilling, A. D., Horiuchi, D., Lively, C. M. & Saxton, W. M. Kinesin-1 and Dynein are the primary motors for fast transport of mitochondria in Drosophila motor axons. Mol. Biol. Cell 17, 2057–2068 (2006).
Kruppa, A. J. & Buss, F. Motor proteins at the mitochondria–cytoskeleton interface. J. Cell Sci. 134, jcs226084 (2021).
Baltrusaitis, E. E. et al. Interaction between the mitochondrial adaptor MIRO and the motor adaptor TRAK. J. Biol. Chem. 299, 105441 (2023).
Canty, J. T., Hensley, A., Aslan, M., Jack, A. & Yildiz, A. TRAK adaptors regulate the recruitment and activation of dynein and kinesin in mitochondrial transport. Nat. Commun. 14, 1376 (2023).
Lorenzo, D. N. et al. A PIK3C3–Ankyrin-B–Dynactin pathway promotes axonal growth and multiorganelle transport. J. Cell Biol. 207, 735–752 (2014).
Eschbach, J. & Dupuis, L. Cytoplasmic dynein in neurodegeneration. Pharmacol. Ther. 130, 348–363 (2011).
Hafezparast, M. et al. Mutations in dynein link motor neuron degeneration to defects in retrograde transport. Science 300, 808–812 (2003).
Wynshaw-Boris, A. & Gambello, M. J. LIS1 and dynein motor function in neuronal migration and development. Genes Dev. 15, 639–651 (2001).
Tammineni, P. & Cai, Q. Defective retrograde transport impairs autophagic clearance in Alzheimer disease neurons. Autophagy 13, 982–984 (2017).
Vicario-Orri, E., Opazo, C. M. & Munoz, F. J. The pathophysiology of axonal transport in Alzheimer’s disease. J. Alzheimer’s Dis. 43, 1097–1113 (2015).
Trimouille, A. et al. An in-frame deletion in BICD2 associated with a non-progressive form of SMALED. Clin. Neurol. Neurosurg. 166, 1–3 (2018).
Koboldt, D. C., Waldrop, M. A., Wilson, R. K. & Flanigan, K. M. The genotypic and phenotypic spectrum of BICD2 variants in spinal muscular atrophy. Ann. Neurol. 87, 487–496 (2020).
Rossor, A. M. et al. Loss of BICD2 in muscle drives motor neuron loss in a developmental form of spinal muscular atrophy. Acta Neuropathol. Commun. 8, 1–12 (2020).
Rudnik‐Schöneborn, S. et al. Autosomal dominant spinal muscular atrophy with lower extremity predominance: a recognizable phenotype of BICD2 mutations. Muscle Nerve 54, 496–500 (2016).
Neveling, K. et al. Mutations in BICD2, which encodes a golgin and important motor adaptor, cause congenital autosomal-dominant spinal muscular atrophy. Am. J. Hum. Genet. 92, 946–954 (2013).
Picher-Martel, V., Morin, C., Brunet, D. & Dionne, A. SMALED2 with BICD2 gene mutations: report of two cases and portrayal of a classical phenotype. Neuromuscul. Disord. 30, 669–673 (2020).
Ueda, Y., Suganuma, T., Narumi-Kishimoto, Y., Kaname, T. & Sato, T. A case of severe autosomal dominant spinal muscular atrophy with lower extremity predominance caused by a de novo BICD2 mutation. Brain Dev. 43, 135–139 (2021).
Fleury, P. & Hageman, G. A dominantly inherited lower motor neuron disorder presenting at birth with associated arthrogryposis. J. Neurol. Neurosurg. Psychiatry 48, 1037–1048 (1985).
Frijns, C., Van Deutekom, J., Frants, R. & Jennekens, F. Dominant congenital benign spinal muscular atrophy. Muscle Nerve 17, 192–197 (1994).
Lunn, M. R. & Wang, C. H. Spinal muscular atrophy. Lancet 371, 2120–2133 (2008).
Harms, M. et al. Dominant spinal muscular atrophy with lower extremity predominance: linkage to 14q32. Neurology 75, 539–546 (2010).
Tekin, H. G., Edem, P. & Özyılmaz, B. Spinal muscular atrophy with predominant lower extremity (SMA-LED) with no signs other than pure motor symptoms at the intersection of multiple overlap syndrome. Brain Dev. 44, 294–298 (2022).
Peeters, K. et al. Novel mutations in the DYNC 1 H 1 tail domain refine the genetic and clinical spectrum of dyneinopathies. Hum. Mutat. 36, 287–291 (2015).
Mei, Y., Jiang, Y., Zhang, Z. & Zhang, H. Muscle and bone characteristics of a Chinese family with spinal muscular atrophy, lower extremity predominant 1 (SMALED1) caused by a novel missense DYNC1H1 mutation. BMC Med. Genomics 16, 47 (2023).
Chan, S. H. S. et al. A recurrent de novo DYNC1H1 tail domain mutation causes spinal muscular atrophy with lower extremity predominance, learning difficulties and mild brain abnormality. Neuromuscul. Disord. 28, 750–756 (2018).
Derksen, A. et al. A novel de novo variant in DYNC1H1 causes spinal muscular atrophy lower extremity predominant in identical twins: a case report. Child Neurol. Open 8, 2329048X211027438 (2021).
Li, J. T. et al. Expanding the phenotypic and genetic spectrum of neuromuscular diseases caused by DYNC1H1 mutations. Front. Cell. Neurosci. 13, 943324 (2022).
Scoto, M. et al. Novel mutations expand the clinical spectrum of DYNC1H1-associated spinal muscular atrophy. Neurology 84, 668–679 (2015).
Marzo, M. G. et al. Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunction. Elife 8, e47246 (2019).
Viollet, L. M. et al. A novel pathogenic variant in DYNC1H1 causes various upper and lower motor neuron anomalies. Eur. J. Med. Genet. 63, 104063 (2020).
Qi, N., Xingxia, W., Mingchao, S. & Qingwen, J. A novel mutation causing spinal muscular atrophy with lower extremity predominance. Neurol. Genet. 1, e20 (2015).
Strickland, A. V. et al. Mutation screen reveals novel variants and expands the phenotypes associated with DYNC1H1. J. Neurobiol. 262, 2124–2134 (2015).
Amabile, S. et al. DYNC1H1-related disorders: a description of four new unrelated patients and a comprehensive review of previously reported variants. Am. J. Hum. Genet. 182, 2049–2057 (2020).
Fiorillo, C. et al. Novel dynein DYNC1H1 neck and motor domain mutations link distal spinal muscular atrophy and abnormal cortical development. Hum. Mutat. 35, 298–302 (2014).
Ding, F. J., Lyu, G. Z., Zhang, V. W. & Jin, H. Missense mutation in DYNC1H1 gene caused psychomotor developmental delay and muscle weakness: A case report. World J. Clin. Cases 9, 9302–9309 (2021).
Martinez Carrera, L. A. et al. Novel insights into SMALED2: BICD2 mutations increase microtubule stability and cause defects in axonal and NMJ development. Hum. Mol. Genet. 27, 1772–1784 (2018).
Asselin, L. et al. Mutations in the KIF21B kinesin gene cause neurodevelopmental disorders through imbalanced canonical motor activity. Nat. Commun. 11, 2441 (2020).
Baron, D. M. et al. ALS-associated KIF5A mutations abolish autoinhibition resulting in a toxic gain of function. Cell Rep. 39, 110598 (2022).
Budaitis, B. G. et al. Pathogenic mutations in the kinesin-3 motor KIF1A diminish force generation and movement through allosteric mechanisms. J. Cell Biol. 220, e202004227 (2021).
Chiba, K. et al. Disease-associated mutations hyperactivate KIF1A motility and anterograde axonal transport of synaptic vesicle precursors. Proc. Natl Acad. Sci. USA 116, 18429–18434 (2019).
Morikawa, M. et al. A neuropathy‐associated kinesin KIF1A mutation hyper‐stabilizes the motor‐neck interaction during the ATPase cycle. EMBO J. 41, e108899 (2022).
Firestone, A. J. et al. Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein. Nature 484, 125–129 (2012).
Steinman, J. B. et al. Chemical structure-guided design of dynapyrazoles, cell-permeable dynein inhibitors with a unique mode of action. eLife 6, e25174 (2017).
Santarossa, C. C. et al. Targeting allostery in the Dynein motor domain with small molecule inhibitors. Cell Chem. Biol. 28, 1460–1473.e1415 (2021).
Höing, S. et al. Dynarrestin, a novel inhibitor of cytoplasmic dynein. Cell Chem. Biol. 25, 357–369.e356 (2018).
See, S. K. et al. Cytoplasmic dynein antagonists with improved potency and isoform selectivity. ACS Chem. Biol. 11, 53–60 (2016).
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This work was supported by the research support program of the Lim Sung Ki Foundation (LF-RSP2022-02).
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Park, JG., Jeon, H., Hwang, K.Y. et al. Cargo specificity, regulation, and therapeutic potential of cytoplasmic dynein. Exp Mol Med 56, 827–835 (2024). https://doi.org/10.1038/s12276-024-01200-7
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DOI: https://doi.org/10.1038/s12276-024-01200-7
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