Abstract
Hematopoietic stem cells (HSCs) possess the potential for self-renew and the capacity, throughout life, to differentiate into all blood cell lineages. Yet, the mechanistic basis for HSC development remains largely unknown. In this study, we characterized a zebrafish smu471 mutant with hematopoietic stem/progenitor cell (HSPC) defects and found that sart3 was the causative gene. RNA expression profiling of the sart3smu471 mutant revealed spliceosome and p53 signaling pathway to be the most significantly enriched pathways in the sart3smu471 mutant. Knock down of p53 rescued HSPC development in the sart3smu471 mutant. Interestingly, the p53 inhibitor, mdm4, had undergone an alternative splicing event in the mutant. Restoration of mdm4 partially rescued HSPC deficiency. Thus, our data suggest that HSPC proliferation and maintenance require sart3 to ensure the correct splicing and expression of mdm4, so that the p53 pathway is properly inhibited to prevent definitive hematopoiesis failure. This study expands our knowledge of the regulatory mechanisms that impact HSPC development and sheds light on the mechanistic basis and potential therapeutic use of sart3 in spliceosome-mdm4-p53 related disorders.
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Introduction
Hematopoiesis is tightly regulated by multidimensional pathways [1,2,3], with dysregulation resulting in anemia, leukemia, and other hematological diseases [4, 5]. Blood development in vertebrates is comprised of primitive and definitive waves [6]. In mammals, the first hematopoietic wave takes place in the yolk sac and gives rise to primitive erythrocytes and macrophages that support tissue oxygenation and immune defense during early embryonic development [1, 7]. Primitive hematopoiesis is transitory and followed by definitive hematopoiesis, which includes yolk sac-derived erythroid-myeloid progenitors. These progenitors contribute to the intermediate wave [8], with later hematopoietic stem cells (HSCs) contributing to hematopoiesis throughout life [9]. Definitive HSCs, which can give rise to all blood lineages, originate from an endothelial-to-hematopoietic transition process within the aorta-gonad mesonephros (AGM) region [10, 11]. These cells migrate to the fetal liver and colonize bone marrow (BM), where they maintain adult hematopoiesis [12, 13]. Similarly, zebrafish hematopoiesis is also comprised of a first primitive wave, a transient erythroid-myeloid progenitor wave, and a definitive HSC contributed wave [14]. HSCs emerge from the ventral wall of the dorsal aorta (VDA, equivalent to the mammalian AGM) and enter the circulation after initiation of the endothelial-to-hematopoietic transition process [15, 16]. The cells colonize the caudal hematopoietic tissue (CHT, equivalent to the mammalian fetal liver) [17, 18], then migrate to adult hematopoietic organs, which include the thymus as well as kidney marrow (equivalent to the mammalian BM) [1]. As HSCs are the apex of the blood hierarchy and have the potential to self-renew and differentiate into all blood cell lineages [19, 20], analysis of HSC biology is essential for a better understanding of hematopoiesis and the treatment of blood disorders.
In the past few decades, spliceosome genes most frequently involved in hematopoiesis and leukemogenesis have been identified including; U2AF1, SF3B1, and SRSF2 [21,22,23]. Mice with the U2AF1 (S34F) mutation exhibit a defect in hematopoiesis as well as an alteration in pre-mRNA splicing in BM progenitors [24]. Mice bearing the Sf3b1K700E mutation exhibit decreased numbers of mature erythrocytes and long-term HSC expansion [25]. Mice bearing the Srsf2P95H mutation have key hematopoietic regulator splicing mistakes as well as impaired hematopoietic differentiation [26]. These mutations occur in myelodysplastic syndrome, chronic myelomonocytic leukemia, and acute myeloid leukemia, which can also be used as prognostic biomarkers of disease [22, 23]. Moreover, several studies have shown these spliceosome genes (such as SF3B1) to be potential antitumor targets [25, 27,28,29]. Therefore, exploring novel spliceosome genes and their role in hematopoiesis will benefit the development of new therapeutic strategies for treatment of blood diseases.
Squamous cell carcinoma antigen recognized by T cells 3 (SART3), also known as p110/p110nrb [30] or TIP110 [31], is a homolog of yeast spliceosome recycling factor Prp24 [32] and an RNA-binding protein [30]. Similar to yeast Prp24, mammalian SART3 likely reassembles U4/U6 small nuclear ribonucleoproteins (snRNPs), affecting pre-mRNA splicing by binding to U6 snRNP [32,33,34]. Moreover, SART3 is multifunctional with involvement in gene regulation [35,36,37], cancer immunology [38,39,40], stem cell pluripotency maintenance [41, 42], embryonic development [43], and hematopoiesis [36, 43, 44]. During BM hematopoiesis, Sart3 haplo-insufficient mice exhibit decreased numbers of hematopoietic progenitor cells (HPCs) and SART3 transgenic mice exhibit increased HPCs, likely due to cell cycle regulation mediated by CMYC [36]. Trede et al. reported a zebrafish sart3 mutant with a lymphoid cell deficiency but normal levels of primitive erythrocytes [43], supporting sart3 involvement in hematopoiesis. However, the role of SART3, in early hematopoietic stem/progenitor cell (HSPC) development, is largely undefined.
In this study, we characterized a zebrafish sart3smu471 mutation, which resulted in a deficiency of HSPC development largely due to activation of the p53 pathway. Moreover, we found that sart3 mutation-induced global splicing changes that included alternative splicing (AS) of mdm4, one key p53 negative regulator, which triggered p53 activation, impacting HSPC proliferation and maintenance. Taken together, this study identifies a novel role for splicing factor and sart3 in regulation of early HSPC development.
Results
The smu471 mutant is defective in HSPC development
To explore novel genes and pathways involved in definitive hematopoiesis, we previously performed an N-ethyl-N-nitrosourea-based large-scale forward genetics screening to identify mutants with hematopoietic defects [45]. One of the mutants, smu471, did not display the erythroid marker (βe1-globin) or the lymphoid marker (rag1) at 5 days post fertilization (dpf), but the HSPC marker (cmyb) was observed at 36 h post fertilization (hpf) [45] (Fig. S1). Next, primitive hematopoiesis was examined. The erythrocyte progenitor marker gata1 (Fig. S2A) and the myeloid progenitor marker pu.1 (Fig. S2B, C) were intact, suggesting that primitive hematopoiesis was unaffected by the mutation. Since definitive HSPCs can differentiate into all blood lineages, both 5-dpf erythroid and lymphoid disruption in smu471 prompted us to speculate that HSPCs were likely defective in the mutant. HSPCs are generated from the VDA ~30 hpf [15, 16] and then migrate, differentiate, and expand in the CHT [1, 17, 18]. We therefore examined the HSPCs in the respective regions for HSPC marker (cmyb, runx1, and gata2b) expression at 36 hpf and 2 dpf in the smu471 mutant. The results showed that cmyb- (Fig. 1A and Fig. S1C), runx1- (Fig. S3A, B), and gata2b- (Fig. S3C, D) marked HSPCs in the VDA region, were similar to siblings of the smu471 mutant. Notably, cmyb expression was detected at 2.5 dpf (Fig. 1B), 3 dpf (Fig. 1C), and 4 dpf (Fig. 1D) in the CHT region but was obviously decreased in the smu471 mutant from 2.5 dpf onward. Since the Tg(cd41:eGFP) transgenic fish line labels developing HSPCs in the VDA and CHT region, with eGFPlow cells as HSPCs and eGFPhigh cells as thrombocytes [46], we monitored VDA and CHT region HSPC number using the inter-crossed progenies of the sart3+/smu471;Tg(cd41:eGFP)+/+ transgenic zebrafish. The results showed that the cd41:eGFPlow cells were intact at 2 dpf in the VDA and CHT region, but were decreased from 2.5 to 4 dpf in the CHT region in smu471 mutants compared with their siblings (Fig. S4A–G), which were consistent with the WISH expression of runx1, gata2b, and cmyb. Moreover, we found the proportion of cd41:eGFPlow cell population at 4 dpf in smu471 mutants was markedly decreased compared with their siblings using Fluorescence-activated cell sorting (FACS) (Fig. S4H, I). These results suggest that HSPCs arise normally from the VDA region whereas CHT localized HSPCs are affected in the smu471 mutant.
Sart3 is the causative gene of the smu471 mutation
To identify the causative gene responsible for the defective HSPCs of the smu471 mutant, we performed positional cloning. We located the mutant gene on chromosome 5 by initial mapping. Next, we used fine mapping to identify the candidate interval between two simple sequence length polymorphism (SSLP) markers: z20360 and z21082, which contained 20 candidate genes (Fig. 2A). By sequencing the cDNA of these candidate genes, we found a T to A base substitution at sart3 exon 6, which encoded a stop codon (Fig. 2B). The nonsense mutation predicted a truncated Sart3 protein lacking its RNA recognition motif (RRM) domain (Fig. 2C).
To confirm sart3 as the causative gene of the smu471 mutant, we performed a rescue experiment by overexpressing zebrafish sart3 mRNA in smu471 mutant embryos. Zebrafish sart3 mRNA recovered cmyb and rag1 expression of the smu471 mutant (Fig. 2D, S5A). Furthermore, human SART3 mRNA also rescued the hematopoietic defect of the smu471 mutant (Fig. S5B), suggesting functional conservation across species. Notably, we generated another sart3 mutant zebrafish by CRISPR/Cas9, which had 14-base pairs deleted and 4-base pairs added in exon 1, identified as sart3(Δ14,+4) (Fig. 2E). The mutant predictably generated a premature Sart3 protein (Fig. 2F) that exhibited the same phenotype as the smu471 mutant (Fig. 2G). Moreover, we outcrossed heterozygous smu471 with heterozygous sart3+/(Δ14,+4) producing a bi-allelic mutant sart3(Δ14,+4)/smu471, which had a similar HSPC defect as the smu471 mutant (Fig. 2H). Taken together, these data indicate that sart3 is the causative gene of the smu471 mutant zebrafish (named sart3smu471 hereafter).
HSPCs undergo arrested proliferation and increased apoptosis in the sart3 smu471 mutant
Based on the published time-series RNA-seq data of the zebrafish CHT region [47] (http://www.picb.ac.cn/hanlab/ichtatlas/Home/), we found the dynamic expression of sart3 to gradually increase with HSPC developmental stage. The sart3 expression in HSPC was low at HSPC initiation (28–36 hpf) and gradually increased from 52 hpf to 4 dpf (Fig. S6), suggesting a role for sart3 in HSPC expansion and maintenance within the CHT region.
We thereby examined cell proliferation and cell death in sart3smu471 mutant HSPCs in the CHT region. We monitored CHT region HSPC status using 2.5-dpf inter-crossed progenies of the sart3+/smu471;Tg(cd41:eGFP)+/+ transgenic zebrafish. Measurement of cell proliferation by the bromodeoxyuridine (BrdU) incorporation assay found the percentage of BrdU labeled cd41:eGFPlow HSPCs (eGFPlow; BrdU double positive cells) to be significantly reduced in sart3smu471 mutants compared to their sibling controls (Fig. 3A, B), suggesting that HSPC proliferation was attenuated by the sart3 mutation. We then monitored cell death in the sart3smu471 mutant by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) assay. Results showed the percentage of cd41:eGFPlow and TUNEL double positive cells to be significantly increased (Fig. 3C, D), suggesting that HSPC maintenance was also affected by the sart3 mutation. Taken together, the above data suggest that the HSPC deficiency in sart3smu471 mutant is due to both decreased cell proliferation and increased cell death.
The HSPC defect in the sart3 smu471 mutant is p53 dependent
Previous analysis has shown SART3 to control CMYC and GATA2 expression during mouse BM hematopoiesis [36], which could partially explain adult HPC cell cycle defects caused by a Sart3 deficiency. Yet, how Sart3 regulates embryonic HSPC proliferation and maintenance is still unclear. To analyze the molecular mechanism, we performed RNA sequencing (RNA-seq) analysis to assess Sart3 regulation of HSPC expansion. Since the HSPC defect was evident at 4 dpf, RNA from sart3smu471 mutants and their wild-type siblings at this stage was collected for sequencing. Distance heatmap analysis of siblings and sart3smu471 mutants showed correlations for each biological replicate. Siblings and sart3smu471 mutants could be divided into two clusters (Fig. 4A). Through Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, we found that the ‘spliceosome’ and the ‘p53 signaling pathway’ were the most significantly enriched pathways in sart3smu471 mutants compared with their siblings (Fig. 4B). Specifically, we found a series of genes (ccng1, p21, gadd45, baxa, casp8, p53, and mdm2) that were upregulated by sart3 mutation (Fig. 4C), all of which were related to p53 involved cell cycle or apoptosis pathways. To validate the RNA-seq data, we evaluated gene expression by real-time quantitative polymerase chain reaction (RT-qPCR). Consistent with the RNA-seq data, expression of p53-related genes was significantly elevated in sart3smu471 mutants compared with siblings (Fig. 4D), confirming activation of the p53 pathway in the sart3smu471 mutants. To determine whether p53 pathway activation occurred in mutant HSPCs, we examined p53-related upregulated gene expression in sorted cd41:eGFPlow labeled HSPCs. We found the expression of these genes to be significantly upregulated in the HSPCs of sart3smu471 mutants compared with their siblings (Fig. 4E), indicating the HSPC defect to be associated with p53 pathway activation.
We also found the level of p53 protein to be increased in sart3smu471 mutants compared with their siblings (Fig. 4F), confirming the p53 activation by sart3 mutation. It is reported that p53 pathway activation is associated with cell cycle arrest and apoptosis [48]. Furthermore, our results suggest that sart3 may regulate HSPC proliferation and apoptosis through activation of the p53 pathway. To assess this possibility, we outcrossed a sart3 heterozygote with a p53M214K mutant in which p53 function was reported to be abrogated [49]. Unfortunately, we failed to obtain a double mutant because of the short distance between sart3 and p53 genes on chromosome 5. As an alternative, we knocked down p53 in sart3smu471 mutants by p53 morpholino oligonucleotide (MO). As expected, downregulation of p53 rescued the HSPC (Fig. 4G) and lymphoid defect (Fig. 4H) in sart3smu471 mutants. Collectively, these results demonstrate that sart3 regulates HSPC development through the p53 pathway.
sart3 mutation induces AS of mdm4
Previous studies have demonstrated SART3 to promote assembly of U4/U6 snRNPs [32, 50]. Deletion of SART3 reduces functional U4/U6 di-snRNPs which have an essential role in pre-mRNA splicing [32]. To assess sart3 regulation of HSPC development, we completed AS analysis based on RNA-seq data. We identified 2075 sart3-affected AS events including; 1327 skipped exons, 159 retained introns, 145 alternative 3′ splice sites, 81 alternative 5′ splice sites, and 363 mutually exclusive exons (Fig. S7A). To determine whether sart3-affected AS events associated with p53 activation, we analyzed genes differentially expressed in the sart3smu471 mutant (5424 genes) with AS (2002 genes) and found 607 genes that overlapped (Fig. 5A). Based on the overlapped genes, we performed Gene Ontology (GO) term enrichment analysis and KEGG pathway analysis using the Metascape database [51] and found ‘mRNA processing’ to be the most enriched pathway (Fig. 5B). The ‘p53 signaling pathway’ was also enriched (Fig. 5B). Clustering analysis of the enriched p53 signaling pathway showed that seven genes (ptenb, adgrg6, thbs1b, adgrb1a, ccnd2a, p53, and baxa) were upregulated and six genes (ddb2, tek, elmo1, mdm4, rrm2, and syk) were downregulated (Fig. 5C). Of note, based on replicate multivariate analysis of transcript splicing (rMATS) analysis, mdm4 in sart3smu471 mutants lacked exon 6 (Fig. S7B), which would result in a truncated mdm4 transcript (mdm4-S) [52]. After analysis of the mdm4 transcript ratio between the mdm4 full-length transcript (mdm4-FL) and mdm4-S, we found that mdm4-FL was decreased in sart3smu471 mutants compared with siblings, whereas the mdm4-S was increased (Fig. 5D). By monitoring expression of mdm4-FL in sart3 mutants, we found levels to be decreased at 2.5 and 4 dpf (Fig. 5E), which suggested an insufficiency of the correct mdm4 transcripts during zebrafish development.
MDM4 was known to inhibit the transcription of p53 by binding its transactivation domain [53], and promote p53 protein degradation by interacting with MDM2 [54]. Besides, p53 could regulate itself transcription [55]. Thus, we speculated that elevated p53 expression and enhanced protein activity in sart3smu471 mutants were attributed to AS and downregulation of mdm4. To confirm the role of mdm4 in sart3-regulation of HSPC development, we performed a functional assay that restored mdm4 in sart3smu471 mutants. We found that decreased HSPCs in sart3smu471 mutants could be partially rescued by overexpressing mdm4 (Fig. 5F, G), suggesting that HSPC deficiency due to sart3 mutation was, at least in part, a result of mdm4 insufficiency.
Discussion
In this study, we mapped the zebrafish smu471 mutation and identified a sart3 nonsense mutant that disrupted early HSPC development resulting in definitive hematopoiesis failure. Furthermore, we showed that splicing dysregulation and p53 pathway activation resulted in definitive hematopoiesis failure in sart3smu471 mutants. Overall, these results demonstrate that impairment of mdm4 by sart3 mutation activates p53 and its downstream pathway, affecting HSPC proliferation and apoptosis during early hematopoiesis (Fig. 6).
During adult hematopoiesis, mouse Sart3 has been associated with HPC numbers, survival, and cell cycling, which are likely linked to regulation of CMYC [36]. In early hematopoiesis, study of the zebrafish egy mutation (another sart3 zebrafish mutant) found that sart3 mutation resulted in multi-organ defects including a definitive hematopoiesis defect in which the T cell marker (rag1) was absent but primitive erythropoiesis intact [43]. Whether and how developing HSPCs of the egy mutant are affected remain unknown. In the present study, we report the smu471 zebrafish to be a different allele of the sart3 mutant. The smu471 mutation exhibited microcephaly and microphthalmia at 4 dpf with death at ~8 dpf (data are not shown). Notably, the smu471 mutant had definitive hematopoiesis failure identical to the egy mutant. Primitive hematopoiesis and definitive HSPC initiation were not disrupted. We attributed definitive hematopoiesis failure to decreased proliferation and increased apoptosis of HSPCs by the smu471 mutation. Taken together, our results and those of others identify a critical role for sart3 in various HSPC development and maintenance stages.
We found the ‘spliceosome’ and the ‘p53 signaling pathway’ to be the most enriched pathways in the sart3smu471 mutant by KEGG pathway enrichment analysis of the RNA-seq data. Consistent with our findings, previous microarray analysis of the egy mutant showed that half of upregulated genes were snRNP encoding genes and splicing-related factors [43]. Similarly, we also noticed p53 upregulated in egy mutant. These observations suggest that both egy and smu471 sart3 mutation alleles affect hematopoietic development by control of similar molecular pathways.
It is well-known that p53 plays essential roles in cell cycle control and cell death [56, 57]. Further, p53 is highly expressed in HSPCs, wherein it mediates quiescence, self-renewal, and apoptosis [58, 59]. Knock down of SART3 in hypoxic human U2OS cancer cells significantly decreased p53 protein levels, and p53 could activate SART3 transcription [37]. However, in whole zebrafish larvae and in endogenous HSPCs, p53 and its regulated genes were elevated by sart3 mutation. In addition, downregulation of p53 efficiently restored HSPC numbers in zebrafish. Reduced HSPC levels were due to decreased cell proliferation and increased apoptosis in the sart3smu471 mutant. Our speculation is that sart3 maintenance of HSPC development is due to p53-dependent inhibition of cellular proliferation and enhanced apoptosis.
Sart3 is known to be a recycling factor for spliceosome assembly [43]. The ‘spliceosome’ is also the most enriched pathway in the sart3smu471 mutant RNA profile. The sart3smu471 mutant exhibited a transcriptome-wide RNA splicing defect. Several reports suggest that p53 activation may be a consequence of a spliceosome defect [60,61,62], with our results providing new evidence supporting this hypothesis. It is known that MDM4 could inhibit the transcription of p53 and stimulate the MDM2-mediated protein degradation of p53 [53, 54]. We found mdm4 to undergo significantly reduced expression along with disrupted AS. Mdm4 expression was changed by sart3 mutation, which is consistent with a report demonstrating reduced mdm4 expression to interfere with the spliceosome [60]. Zebrafish AS of mdm4 in which exon 6 is skipped in the sart3smu471 mutant is similar to another HSPC deficient zebrafish spliceosome mutant, bcas2−/− [62] and to the skipping of exon 6 in human or exon 7 in mouse [63, 64]. A truncated transcript termed mdm4-S was previously identified [52]. The conservation of spliceosome-affected mdm4 and p53 activation across species suggests a general spliceosome-mdm4-p53 regulatory pathway in vertebrates.
Moreover, SART3 alteration may be a favorable index for cancer diagnosis, prognosis, and therapy. For example, SART3 expression may be a diagnostic and prognostic indicator of melanoma [65]. Antisense oligonucleotides-mediated MDM4 exon 6 skipping could prove useful as a therapeutic strategy for breast carcinoma, melanoma, and diffuse large B cell lymphoma (DLBCL), by decreasing MDM4 abundance and reducing the growth of these tumors [63]. Interestingly, we found several DLBCL cases with SART3 alteration in the cBioPortal database (data are not shown). Thus, whether SART3 is a candidate prognostic marker or a therapeutic target for DLBCL is worth future investigation. We have shown that human SART3 mRNA rescues the zebrafish sart3smu471 mutant (Fig. S5B). The sart3smu471 zebrafish could serve as an in vivo model for functional validation of patient-derived mutations as well as a tool for cancer therapeutic approach development.
Taken together, this study describes a novel function for sart3 in which HSPC proliferation and apoptosis are regulated. Further, we found that AS disruption in the sart3smu471 mutant included effects on the p53 repressor, mdm4, which activated p53. Activated p53 was triggered by mdm4 exon 6 skipping and therefore sart3 mutation may be a favorable prognostic marker or a potential therapeutic target for some cancers. This study expands understanding of the mechanisms that regulate HSPC development and sheds light on the mechanistic basis and potential therapeutic treatments for sart3 related disorders.
Materials and methods
Zebrafish strains
Zebrafish were raised and maintained in standard conditions [66] with a 14-h light/10-h dark cycle at 28.5 °C. The following zebrafish strains were used; AB, WIK, Tg(cd41:eGFP) [67], sart3smu471, and p53M214K [49].
Genetic mapping and positional cloning
Mapping family generation and positional cloning were performed as previously described [68]. Heterozygous smu471 [45] were outcrossed to the mapping strain WIK to generate the F1 mapping population, which would be further intercrossed to generate F2 gynogenetic diploid offspring. To verify the mutant phenotype, F2 individuals were subjected to whole mount in situ hybridization (WISH) by rag1 antisense probe at 5 dpf for genetic mapping. The candidate interval was flanked by two SSLP markers: z20360 and z21082. The 20 candidate genes in this region were all subjected to exon region sequencing in both siblings and smu471 mutants.
Generation of sart3 mutants by CRISPR/Cas9
sart3(Δ14,+4) mutants were generated using the CRISPR/Cas9 system [69]. The guide RNA (gRNA) targeting sequence was as follows: 5′-GGAGCGAGAGATGGAGTCCG-3′, with the gRNA synthesized using T7 RNA polymerase (EP0111, Thermo Fisher Scientific, San Jose, CA, USA). The mixture of gRNA target (500 ng/µL) and Cas9 protein (600 ng/µL) (M0646M, New England Biolabs, Ipswich, MA, USA) was co-injected into one-cell stage embryos and the mutation efficiency assessed by T7E1 (M0302S, New England Biolabs) digestion. The injected embryos were raised to sexual maturity and screened for a stable F2 heterozygous phenotype of interest.
Microinjection of mRNAs, plasmids, and MO
The coding sequence of sart3 was PCR amplified and cloned into the pCS2+ vector, then the sart3 mRNA was synthesized in vitro by mMESSAGE mMACHINE™ SP6 in vitro Transcription Kit (AM1340, Invitrogen, Carlsbad, CA, USA). The coding sequence of mdm4 was PCR amplified and cloned into the pTol2-hsp 70 vector. The p53 MO (5′-GCGCCATTGCTTTGCAAGAATTG-3′) was ordered from Gene Tools as described previously [70]. Microinjection was conducted with 1.5 μg/μL sart3 mRNA, 70 ng/μL pTol2-hsp 70: mdm4, and 1 mM p53 MO. The construction primers of sart3 and mdm4 used in this study are listed in Table S1.
WISH and genotyping
WISH was performed as described previously [71]. After imaging, the DNA of whole embryo/larva was extracted for genotyping to distinguish sart3smu471 mutants and siblings. PCR was performed to obtain DNA fragments including the point mutation site (forward primer: 5′-GACTGGGCAGATGATGGCGT-3′; reverse primer: 5′-CACCGCATCTGCTCCCAGAGAC-3′), then the PCR products were analyzed by high-resolution melting (HRM) genotyping, with the wild type (WT) and mutant showing one peak and the heterozygote showing two different peaks to initially distinguish those that were heterozygous. Next, we added pre-prepared WT PCR products to the one peak samples for second round HRM analysis, such that the WT showed one peak while the mutant showed two different peaks, as heterozygous.
Antibody staining
Progenies of the intercrossed sart3+/smu471;Tg(cd41:eGFP)+/+ transgenic zebrafish were fixed and antibody staining was performed as previously described [72]. Primary antibody anti-GFP (1:400, ab6658, Abcam, Cambridge, UK) and secondary antibody Alexa Fluor 488-conjugated anti-goat (1:400, A32814, Invitrogen) were used. Images were captured using a Zeiss LSM800 laser scanning confocal microscope and cd41:eGFPlow labeled HSPCs were distinguished from cd41:eGFPhigh labeled thrombocytes [46] by the Range Indicator tool of ZEN software according to fluorescence intensities.
BrdU incorporation and double staining
Progenies of the intercrossed sart3+/smu471;Tg(cd41:eGFP)+/+ transgenic zebrafish were treated with 10 mM BrdU for 3 h at 28.5 °C. After fixation, dehydration, rehydration, and digestion with proteinase K, BrdU labeled embryos were incubated with anti-BrdU (1:50, 111703760001, Roche, Basel, Switzerland) and anti-GFP (1:400, ab6658, Abcam), then incubated with goat Alexa Fluor 555-conjugated anti-mouse (1:400, A31570, Invitrogen) and donkey Alexa Fluor 488-conjugated anti-goat (1:400, A32814, Invitrogen) secondary antibodies for immunofluorescent staining as previously described [73, 74]. The fluorescent images were captured using a Zeiss LSM800 laser scanning confocal microscope with a dual laser channel. According to fluorescence intensities, cd41:eGFPlow labeled HSPCs were distinguished from cd41:eGFPhigh labeled thrombocytes as mentioned above. Double-positive cells were identified by layer-to-layer examination. Statistical analysis was performed after distinguishing siblings and mutants by HRM genotyping of each sample.
TUNEL immunostaining and double staining
TUNEL was performed with an In Situ Cell Death Detection Kit TMR red (12156792910, Roche) as described [73, 74]. Initially, the sart3+/smu471;Tg(cd41:eGFP)+/+ transgenic zebrafish intercrossed progenies were fixed, dehydrated, rehydrated, and digested with proteinase K. After incubation with permeabilization solution (acetone:ethanol = 1:2) at −20 °C for 7 min, the embryos were rinsed and stained with the Kit according to the manufacturer’s instructions. Imaging, quantification, and statistical analysis were carried out as described above.
RNA-seq and RT-qPCR
Total RNA of desired stage embryos/larvae or sorted-HSPCs was extracted using Trizol reagent (15596026, Invitrogen) according to the manufacturer’s instructions. For RNA-seq, the sequencing libraries were generated by NEBNext® UltraTM RNA Library Prep Kit for Illumina® (E7770, New England Biolabs). For RT-qPCR, cDNA was prepared with HiScript Q RT SuperMix for qPCR (+gDNA wiper) (R323-01, Vazyme, Nanjing, China). The RT-qPCR was performed using PowerUp™ SYBR® Green Master Mix (4472908, Thermo Fisher Scientific) in a LightCycler 96 system (Roche). The RT-qPCR primers used in this study are listed in Table S2.
AS analysis and detection
For AS events analysis, rMATS software was used [75]. For mdm4 AS detection, cDNA of sart3smu471 mutants and their siblings was prepared as above-mentioned, then mdm4 AS was visualized by PCR using cDNA template. AS detection primers used in this study are listed in Table S3.
Western blot
To obtain zebrafish protein, we distinguished 4-dpf sart3smu471 mutants and their siblings based on the microcephaly and microphthalmia phenotype of the sart3smu471 mutant, then extracted total protein for western blot as previously described [76]. Mouse monoclonal antibody anti-p53 (1:200, ab77813, Abcam) [77, 78] and rabbit monoclonal antibody anti-GAPDH (1:1000, #2118, Cell Signaling Technology, Danvers, MA, USA) were used as primary antibodies.
FACS analysis
The sart3+/smu471;Tg(cd41:eGFP)+/+ zebrafish were intercrossed and 4-dpf sart3smu471 mutants as well their siblings were distinguished as mentioned above. The FACS experiment was performed as described previously [79]. As reported, cd41:eGFPlow labeled cells were recognized as HSPCs [46], and were, respectively, analyzed or sorted from sart3smu471 mutants and siblings using a MoFlo™ XDP (Beckman Coulter, Brea, CA, USA).
Statistical analysis
Data were recorded and analyzed using GraphPad Prism 7. Categorical variables were analyzed by Fisher’s exact test. Comparisons between two groups were analyzed by two-tailed Student’s t-test and nonparametric test (Mann–Whitney U test), based on normal distribution data or not, respectively. Data were presented as means ± standard deviation (SD). P < 0.05 was considered statistically significant.
Data availability
The RNA-seq data was submitted to the NCBI GEO repository with the accession number GSE182560.
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Acknowledgements
We thank Dr. Bo Zhang and Dr. Jingwei Xiong for sharing CRISPR/Cas9-related material (gRNA-pMD19-T) and protocols. We thank Dr. Robert I. Handin and Dr. Leonard I. Zon for sharing us Tg(cd41:eGFP) and p53M214K fish lines. We thank Dr. Kun Wang, Mr. Xiaohui Chen, and Ms. Li Song for the animal maintenance and technique assistance.
Funding
This work was supported by the National Key Research and Development Program of China (2018YFA0800200 and 2018YFA0801000), the National Natural Science Foundation of China (31922023), and Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2019).
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Y Zhang supervised this study; ZW and WZ initiated the mutant screening; Y Zhao performed most experiments; Y Zhang, Y Zhao, and MW analyzed the data and wrote the manuscript; JL, JX, and ZH provided technique advice and discussion; and PM and JC helped with the mutant strain maintaining.
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The zebrafish study was reviewed and approved by the animal research advisory committee of South China University of Technology.
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Zhao, Y., Wu, M., Li, J. et al. The spliceosome factor sart3 regulates hematopoietic stem/progenitor cell development in zebrafish through the p53 pathway. Cell Death Dis 12, 906 (2021). https://doi.org/10.1038/s41419-021-04215-4
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DOI: https://doi.org/10.1038/s41419-021-04215-4
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