Abstract
Baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) replicates in both Spodoptera frugiperda Sf21 and Trichoplusia ni Tn368 cells, whereas AcMNPV defective in hcf-1 (host cell-factor 1) gene productively infects only Sf21 cells, indicating that HCF-1 is indispensable for the AcMNPV productive infection of Tn368 cells. Here, we demonstrated that HCF-1 protein transiently expressed in Tn368 cells promotes the DNA synthesis of Hyphantria cunea MNPV (HycuMNPV), Orygia pseudotsugata MNPV and Bombyx mori NPV, which are normally unable to replicate in Tn368 cells. We also demonstrated that a recombinant HycuMNPV harboring the hcf-1 gene successfully replicates in Tn368 cells, generating substantial yields of progeny viruses and polyhedra. These results indicate that HCF-1 encoded by AcMNPV is an essential viral factor for productive NPV infection of Tn368 cells. Taken together with the previous findings on HRF-1 (host range factor 1), the present results provide strong evidence that viral genes acquired through horizontal gene transfer play an important role in baculovirus evolution, serving to expand the host range of baculoviruses.
Similar content being viewed by others
Introduction
Nucleopolyhedroviruses (NPVs), which belong to members of the family Baculoviridae, are large, enveloped, insect-pathogenic viruses that contain a double-stranded circular DNA genome of 80 to 180 kbp1. NPVs generally exhibit a narrow host range and are capable of productively infecting only one or a few closely related insect species. Notably, however, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and Anagrapha falcifera and Rachiplusia ou MNPVs, which are close relatives of AcMNPV, are capable of infecting over 30 lepidopteran insect species within 12 families2,3,4,5.
Several viral genes that determine the host range of NPVs have been identified and include p143, ie-2, lef-7 (late expression factor-7), p35, iaps (inhibitors of apoptosis), host range factor 1 (hrf-1), and host cell-specific factor 1 (hcf-1)6,7,8. The p143, ie-2, lef-7, and hcf-1 play a role in viral DNA replication or late gene expression, whereas the p35 and iaps are involved in an antiviral defense system of insects, serving as anti-apoptotic genes. These genes are differentially required for optimal viral replication in a cell-line specific manner. For example, recombinant AcMNPV defective in functional p35 gene normally replicates in Tn368 cells derived from the cabbage looper Trichoplusia ni, but induces massive apoptosis in Sf21 cells derived from the fall armyworm Spodoptera frugiperda, yielding little or no progeny viruses9, 10. Similarly, AcMNPV harboring p143 gene derived from Bombyx mori NPV in place of its native p143 replicates successfully in non-permissive B. mori BmN-4 or Bm5 cells11, 12. Notably, the HRF-1 and HCF-1 have been shown to contribute to AcMNPV replication exclusively in Lymantria dispar Ld652Y cells and Tn368 cells, respectively13,14,15,16. Despite the identification and characterization of these genes, the molecular mechanisms underlying the host range determination of NPVs remain largely elusive.
AcMNPV replicates to high titers in both Sf21 and Tn368 cells. In recombinant AcMNPV defective in the hcf-1 gene, however, productive infection only occurs in Sf21 cells, indicating that AcMNPV requires HCF-1 for the productive infection of Tn368 cells14, 15. The HCF-1 protein contains a putative RING-finger domain, which is involved in the formation of functional HCF-1 dimers or higher-order structures in the nucleus of infected Tn368 cells17, 18. It was also demonstrated that transiently expressed HCF-1 protein represses expression from the hcf-1 promoter and this repression activity of HCF-1 is required for the efficient expression of viral late genes and production of polyhedra in Tn368 cells18. However, the functional role of HCF-1 protein in AcMNPV-infected Tn368 cells has not been conclusively determined.
In the present study, we demonstrated that transiently expressed HCF-1 protein promotes the productive infection of non-permissive Tn368 cells by Hyphantria cunea MNPV (HycuMNPV). Recombinant HycuMNPV harboring the hcf-1 gene also successfully replicated in Tn368 cells, indicating that hcf-1 gene embedded in the HycuMNPV genome functions effectively for HycuMNPV productive infection of Tn368 cells. In contrast, Orgyia pseudotsugata MNPV (OpMNPV) and Bombyx mori NPV (BmNPV) were capable of viral DNA replication in HCF-1-expressing Tn368 cells, but not the synthesis of viral structural or polyhedral proteins. Taken together, these results indicate that HCF-1 protein is an essential viral factor for the productive NPV infection of Tn368 cells, but is not sufficient to promote viral protein synthesis of certain NPVs in infected Tn368 cells.
Results
HCF-1 promotes viral DNA and viral protein production of certain NPVs in non-permissive Tn368 cells
To determine whether HCF-1 promotes the productive infection of NPVs other than AcMNPV in Tn368 cells, transfection-infection experiments were performed using four different NPVs that are non-permissive in T. ni cells. Tn368 cells were first transfected with plasmids pFBD/hcf-1 and pFBD/luc, which express HCF-1 and luciferase proteins, respectively, under transcriptional control of the Drosophila melanogaster heat shock protein 70 (HSP70) gene promoter (Fig. 1a). At 24 h post-transfection, Tn368 cells were heat-shocked at 42 °C for 30 min and incubated for 6 h at 28 °C. The cells were then infected with HycuMNPV, OpMNPV, BmNPV and Lymantria dispar MNPV (LdMNPV), and examined for viral DNA, viral proteins, progeny budded viruses (BVs) and polyhedra. Microscopic examination at 72 h post-infection showed that polyhedra were only produced in a small number of HycuMNPV-infected Tn368 cells (Fig. 1b). However, progeny BV production by any of the examined NPVs was not detected (Fig. 1c). Although BV production was not observed, viral DNA synthesis was promoted by HCF-1 protein in Tn368 cells infected with HycuMNPV, OpMNPV and BmNPV (Fig. 1d). Notably, the production of major capsid protein VP39 and polyhedrin (matrix protein of polyhedra) were observed clearly only in HycuMNPV-infected Tn368 cells (Fig. 1e). In luciferase-expressing Tn368 cells, no significant increases in viral DNA, VP39 protein, polyhedrin, progeny BVs or polyhedra were detected following infection with any of the examined NPVs (Fig. 1b–e). Immunoblot analysis showed that substantial amounts of luciferase and HCF-1 proteins were expressed in the plasmid-transfected and virus-infected Tn368 cells (Fig. 1f).
Construction and characterization of HycuMNPV bacmid (HycuBac) in SpIm cells
The results of the transfection-infection experiments presented in Fig. 1 suggested that HCF-1 protein promotes productive HycuMNPV replication in non-permissive Tn368 cells. To confirm this speculation, we constructed and characterized a HycuMNPV bacmid (HycuBac; Fig. 2a) in SpIm cells, which are permissive for HycuMNPV. HycuBac DNA or a plasmid that expresses EGFP protein was transfected into SpIm cells. At 72 h post-transfection, a number of polyhedra were found only in HycuBac DNA-transfected SpIm cells (Fig. 2b). Progeny BVs (vHycuBac) from HycuBac DNA-transfected SpIm cells and those of parental HycuMNPV were used to infect SpIm cells to compare the kinetics of polyhedra production, progeny BV yield and polyhedrin expression. All measured kinetic parameters of vHycuBac were comparable to those of parental HycuMNPV in infected SpIm cells up to 72 h post-infection (Fig. 2c–e).
Construction and characterization of recombinant HycuBac harboring hcf-1 or the luciferase gene in SpIm cells
As HycuBac was shown to produce progeny BVs in SpIm cells with biological activity comparable to those of parental HycuMNPV, we constructed recombinant HycuBac harboring hcf-1 (HycuBac/hcf-1) or the luciferase gene (HycuBac/luc) (Fig. 3a) to determine whether HCF-1 protein promoted HycuMNPV replication in Tn368 cells. The two types of progeny BVs, vHycuBac/hcf-1 and vHycuBac/luc, were obtained from SpIm cells transfected with HycuBac/hcf-1 and HycuBac/luc, respectively, and were then used to infect SpIm cells. No significant kinetic differences were observed between vHycuBac/hcf-1 and vHycuBac/luc with respect to polyhedra production, BV yield, and syntheses of envelope fusion protein GP64, major capsid protein VP39 and polyhedrin until 72 h post-infection (Fig. 3b–d). The luciferase and HCF-1 proteins were expressed at high levels in SpIm cells following infection with vHycuBac/hcf-1 and vHycuBac/luc, respectively (Fig. 3e).
Recombinant HycuMNPV harboring hcf-1 (vHycuBac/hcf-1) replicates in Tn368 cells
As no significant differences in the biological properties of vHycuBac/hcf-1, vHycuBac/luc and vHycuBac in SpIm cells were detected (Fig. 3), Tn368 cells were infected with vHycuBac/hcf-1 to determine whether HCF-1 protein expressed from the HycuMNPV genome promoted HycuMNPV replication in Tn368 cells. Microscopic examination showed that polyhedra were first produced at 48 h post-infection and that the number of cells with polyhedra had markedly increased by 72 h post-infection (Fig. 4a). BV production in vHycuBac/hcf-1-infected Tn368 cells increased continuously from 48 to 72 h post-infection (Fig. 4b) and the amount of viral DNA increased strikingly at 24 h post-infection (Fig. 4c). Envelope fusion protein GP64 accumulated substantially in vHycuBac/hcf-1-infected Tn368 cells from 24 h post-infection onwards (Fig. 4d) and VP39 and polyhedrin proteins were clearly detected at 48 h post-infection and remained detectable at 72 h post-infection in vHycuBac/hcf-1-infected Tn368 cells (Fig. 4d). In contrast, no significant production of polyhedra, BVs, viral DNA, VP39 protein or polyhedrin was observed in vHycuBac/luc-infected Tn368 cells (Fig. 4a–d). The GP64 band that was detected temporarily at 24 h post-infection in vHycuBac/luc-infected Tn368 cells (Fig. 4d) represents GP64 protein expressed through the gp64 early promoter, which is occasionally observed upon infection with group I alphabaculoviruses even when inoculated viruses are unable to replicate productively. Immunoblot analysis showed that the luciferase and HCF-1 proteins were expressed at high levels from 24 to 72 h post-infection (Fig. 4e).
Discussion
The hcf-1 gene is required for the efficient replication of AcMNPV in Tn368 cells, but is dispensable for the productive infection of Sf21 cells14, 15, indicating that the HCF-1 functions specifically in Tn368 cells to expand the host range of AcMNPV. In the present study, we examined whether HCF-1 also promotes the replication of HycuMNPV, OpMNPV, BmNPV and LdMNPV in non-permissible Tn368 cells, and found that HycuMNPV harboring the hcf-1 gene replicates in Tn368 cells, producing substantial amounts of BVs and polyhedra. These findings demonstrate that expansion of the AcMNPV host range to Tn368 cells by hcf-1 can also be extended to other NPVs, suggesting that HCF-1 is an essential viral factor for productive NPV infection of Tn368 cells.
The hcf-1 gene of AcMNPV is one of the lef (late expression factor) genes, which is essential for viral DNA replication and expression of late genes in transient assays in Tn368, but not Sf21, cells14. In AcMNPV-infected Tn368 cells, the HCF-1 protein is synthesized early during infection and localizes in punctuate nuclear structures17, 18. Recombinant AcMNPV defective in functional hcf-1 gene replicates normally in Sf21 cells, but exhibits various mutant phenotypes in Tn368 cells, including defective viral DNA replication and late gene transcription, and complete cessation of cellular and viral protein syntheses15. These results collectively imply that the HCF-1 protein is involved in viral DNA replication and late gene expression, as well as cellular protein synthesis, in AcMNPV-infected Tn368 cells. However, the present results also showed that although OpMNPV and BmNPV DNAs were synthesized successfully in HCF-1-expressing Tn368 cells, major capsid protein VP39 and polyhedrin were not detected, and neither BVs nor polyhedra were produced. Quantitative qRT-PCR analysis revealed that transcripts from the vp39 genes of HycuMNPV and OpMNPV, but not those of BmNPV or LdMNPV, increased markedly at 48 h post-infection in HCF-1-expressing Tn368 cells (Supplementary Figure S1). As no vp39 gene transcripts were detected in Tn368 cells infected with BmNPV or LdMNPV, the hcf-1 gene appears to promote viral late gene transcription in an NPV species-specific manner in Tn368 cells. In addition, because the OpMNPV vp39 gene was transcribed at high levels in HCF-1-expressing Tn368 cells, recombinant OpMNPV harboring hcf-1 may also be capable of replicating productively in non-permissive Tn368 cells.
A similar cell-line specific viral factor to HCF-1, designated as HRF-1, was identified in LdMNPV and functions specifically in Ld652Y cells derived from L. dispar 13, 16. The hrf-1 gene was shown to rescue the global translation arrest of Ld652Y cells caused by AcMNPV infection and promotes productive AcMNPV replication in Ld652Y cells13, 16. We previously demonstrated that HRF-1 promotes the replication of BmNPV, HycuMNPV, and Spodoptera exigua MNPV in non-permissive Ld652Y cells, indicating that the HRF-1 protein also functions to expand the host range of various NPVs to non-permissive Ld652Y cells19, 20.
The hcf-1 and hrf-1 genes are encoded by only a few members of the family Baculoviridae. Evidence suggests that the hrf-1 gene was acquired quite recently by LdMNPV through horizontal gene transfer21, and that homologs of HRF-1 with low amino acid sequence identity are found exclusively in OpMNPV13, 22, which productively infects Ld652Y cells23, and Dasychira pudibunda NPV, which is closely related to OpMNPV24. Homologs of hcf-1 have been identified in only four NPV species: three close relatives of AcMNPV (Rachiplusia ou MNPV, Plutella xylostella NPV and Thysanoplusia orichalcea MNPV), and Clanis bilineata NPV, which is more distantly related to AcMNPV8, 25,26,27, suggesting that the hcf-1 gene of AcMNPV was also acquired by a gene acquisition event. Notably, despite marked differences in structure and mode of action in virus-infected cells, HCF-1 and HRF-1 proteins have convergent functional roles in the host range expansion of NPVs to non-permissive cell lines derived from T. ni and L. dispar, respectively. Taken together with previous findings for the hrf-1 gene19, 20, the present study indicates that several NPVs contain genes that are acquired by gene acquisition events and contribute to evolution of baculoviruses in terms of the host range expansion.
Methods
Cells, viruses and virus infection
Tn368 cells derived from Trichoplusia ni 28 were maintained at 28 °C in TC100 medium (AppliChem) supplemented with 0.26% tryptose broth (Sigma) and 10% fetal bovine serum (FBS). FRI-SpIm (SpIm) cells derived from Spilosoma imparilis 29 were grown at 28 °C in MM medium30 supplemented with 3% FBS. The following clonal isolates of four NPVs were used in these experiments: BmNPV N9 (BmNPV) from the silkworm B. mori 31, HycuMNPV N9 (HycuMNPV) from the fall webworm H. cunea 32, LdMNPV A21-MPV (LdMNPV) from the gypsy moth L. dispar 33, and OpMNPV from the Douglass-fir tussock moth, O. pseudotsugata 34.
Tn368 and SpIm cells were infected with viruses at varying multiplicity of infection (MOI) during a 60-min incubation at room temperature, as described previously35. Infected Tn368 and SpIm cells were washed three times with TC100 and MM media, respectively, and used for analyses at the indicated time points. The time zero of infection was defined as the time when the viral inoculum was removed from the cell culture.
Plasmid construction
Plasmids pFBD/hcf-1 and pFBD/luc, which express HCF-1 and luciferase, respectively, under control of the HSP70 promoter (cf., Fig. 1a), were constructed using an In-Fusion HD Cloning Kit (Clontech). Linearized pFBD/pIE1-2/Egfp36 was obtained by inverse PCR using primers P1 and P2 (see Supplementary Table S1 for the primers used in this study). The region containing the HSP70 promoter and N-terminal HA- and His-tagged hcf-1 open reading frame (ORF) was PCR-amplified from pHSHAHishcf-1VI+12 using primers P3 and P4, which contained 15-nt extension sequences complementary to the ends of linearized pFBD/pIE1-2/Egfp. The amplified hcf-1 ORF region was cloned into linearized pFBD/pIE1-2/Egfp using an In-Fusion HD Cloning Kit, yielding pFBD/pIE1-2/Egfp/pHS/HAHisHcf-1 (pFBD/hcf-1). Control plasmid pFBD/pIE1-2/Egfp/pHS/HAHisLuc (pFBD/luc) was constructed by replacing the hcf-1 gene of pFBD/hcf-1 with the luciferase gene that was amplified by PCR from pGL (Promega) using primers P5 and P6. hcf-1-defective linearized pFBD/hcf-1 was obtained by inverse PCR using primers P7 and P2. pFBD/hcf-1 and pFBD/luc were used as expression plasmids and for recombinant HycuMNPV bacmid construction. The construction of pIE1-2/Egfp, which expresses the EGFP protein, was described previously37. The plasmid sequences were confirmed by nucleotide sequencing using a BigDye Terminal v3.1 Cycle Sequencing Ready Reaction Kit and ABI 3130 Genetic Analyzer (Applied Biosystems), as described previously38.
Construction of HycuMNPV bacmids
HycuBac containing a miniF replicon, LacZα gene, Tn7 insertion site and kanamycin-resistance gene (miniF-Kan region) (cf., Fig. 2a) was constructed in SpIm cells by homologous recombination between HycuMNPV genomic DNA and the transfer vector pHycuIE1-miniFKan-IE0 containing a miniF-Kan region. To construct pHycuIE1-miniFKan-IE0, the ie1-ie0 region was PCR-amplified from HycuMNPV genomic DNA using primers P8 and P9, and the obtained amplicon was ligated to EcoRV-digested pBlueScript KS+ (Stratagene), yielding pHycuIE1-IE0. For construction of pHycuIE1-miniFKan-IE0, pHycuIE1-IE0 was linearized by inverse PCR using primers P10 and P11. The miniF-Kan region was PCR-amplified from AcMNPV bacmid DNA (Invitrogen) using primers P12 and P13, which contained 15-nt extension sequences complementary to the ends of linearized pHycuIE1-IE0. The obtained amplicon was cloned into linearized pHycuIE1-IE0 using an In-Fusion HD Cloning Kit, yielding pHycuIE1-miniFKan-IE0.
pHycuIE1-miniFKan-IE0 was co-transfected with HycuMNPV genomic DNA into SpIm cells using Lipofectin (Invitrogen), as described previously39, 40. Viral DNA was extracted from BVs and electroporated into DH10B Escherichia coli cells using a MicroPulser (BioRad). Positive transformants were selected on LB agar containing kanamycin (50 μg/ml), IPTG (24 μg/ml) and X-Gal (60 μg/ml). HycuBac DNA was isolated from LacZ-positive and kanamycin-resistant colonies using a Qiagen Plasmid Midi Kit (Qiagen). HycuBac was then transfected into SpIm cells and culture medium containing vHycuBac BVs was collected at 168 h post-transfection. Working stocks of vHycuBac were amplified in SpIm cells.
Recombinant HycuBac harboring hcf-1 (HycuBac/hcf-1) or luciferase gene (HycuBac/luc) (cf., Fig. 3a) was constructed through Tn7-mediated transposition in DH10B cells. DH10B cells carrying both HycuBac and the helper plasmid pMON7124 (Invitrogen) were transformed with pFBD/hcf-1 or pFBD/luc. The cells were incubated at 37 °C for 5 h and positive transformants were then selected on LB agar containing kanamycin (50 μg/ml), tetracycline (50 μg/ml), gentamicin (10 μg/ml), IPTG (24 μg/ml) and X-Gal (60 μg/ml). HycuBac/hcf-1 and HycuBac/luc DNA was purified from LacZ-negative colonies. The presence of hcf-1 and luciferase with the correct sequence and structure in HycuBac/hcf-1 and HycuBac/luc DNAs, respectively, was verified by PCR analysis and DNA sequencing. BV stocks from HycuBac/hcf-1 (vHycuBac/hcf-1) and HycuBac/luc (vHycuBac/luc) were prepared as described above for vHycuBac.
Immunoblot analysis
Immunoblot analyses of viral structural proteins, polyhedrin, and hemagglutinin (HA)-tagged HCF-1 and luciferase proteins were performed as described previously37, 41. Briefly, polypeptides from virus- and mock-infected cells were resolved on 12% SDS-polyacrylamide gels and blotted onto Immobilon-P (Millipore) or nitrocellulose membranes (Advantec Toyo) using a Trans-Blot Turbo system (Bio-Rad). GP64 and HA-tagged proteins (HCF-1 and luciferase) were probed with monoclonal antibodies against AcMNPV GP64 (Clontech) and HA (HA. 11; Babco), respectively, and were then reacted with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (Zymed Laboratories). VP39 and polyhedrin proteins were probed with polyclonal antibodies against BmNPV/AcMNPV VP3942 and BmNPV polyhedrin proteins35, respectively, and were then reacted with HRP-conjugated goat anti-rabbit IgG antibody (Zymed Laboratories). Positive signals were visualized using ECL Western Blotting Detection Reagent (GE Healthcare), ECL Select Western Blotting Detection Reagent (GE Healthcare) or an HRP Conjugate Substrate Kit (Bio-Rad).
Quantitative PCR (qPCR) analysis
Quantification of ie1 gene copies of HycuMNPV (hycu-ie1), OpMNPV (op-ie1), BmNPV (bm-ie1) and LdMNPV (ld-ie1) was performed by qPCR analysis, as described previously36. Briefly, total DNAs were extracted from virus-infected Tn368 cells using a QIAamp DNA Mini Kit (Qiagen) and subjected to qPCR analysis using a StepOnePlus Real-Time PCR system (Applied Biosystems) with the gene-specific probes Pb-1, Pb-2, Pb-3 and Pb-4 (Supplementary Table S2), and gene-specific primers P14 and P15, P16 and P17, P18 and P19, and P20 and P21 for hycu-ie1, op-ie1, bm-ie1 and ld-ie1, respectively. The number of ie-1 gene copies was determined using a standard curve generated from 10-fold dilutions of plasmids containing partial sequences of each ie-1 ORF (pBS/hycuie1PS, pTOPO/opie1PS, pTOPO/bmie1PS and pTOPO/ldie1PS). The plasmids were constructed by cloning each partial sequence, which was amplified using the primers P22 and P23 for hycu-ie1, P24 and P25 for op-ie1, P26 and P27 for bm-ie1, and P28 and P29 for ld-ie1, into EcoRV-digested pBlueScript KS+ using DNA Ligation Kit Mighty Mix (Takara) or into pCR4-TOPO using a TOPO TA Cloning Kit (Invitrogen).
Quantitative RT-PCR (qRT-PCR) analysis
Quantification of mRNAs expressed from the vp39 genes of HycuMNPV (hycu-vp39), OpMNPV (op-vp39), BmNPV (bm-vp39) and LdMNPV (ld-vp39) was performed by qRT-PCR, as described previously40. Briefly, total RNAs were extracted from virus-infected Tn368 cells using a SYBR Green Cells-to-CT Kit (Ambion), treated with DNase I using an RNAqueous-4PCR Kit (Ambion) and reverse-transcribed to cDNAs using a SYBR Green Cells-to-CT Kit. The resultant cDNAs were subjected to qRT-PCR analysis using a StepOnePlus Real-Time PCR system with the gene-specific primers P30 and P31, P32 and P33, P34 and P35, and P36 and P37 for hycu-vp39, op-vp39, bm-vp39 and ld-vp39, respectively. The number of each vp39 mRNA was calculated using a standard curve generated from 10-fold dilutions of plasmids containing entire or partial sequences of each vp39 ORF (pTOPO/hycuvp39, pTOPO/opvp39, pTOPO/bmvp39 and pTOPO/ldvp39PS). The plasmids were constructed by cloning each sequence using the primers P38 and P39 for hycu-vp39, P40 and P41 for op-vp39, P42 and P43 for bm-vp39, and P37 and P44 for ld-vp39 into pCR4-TOPO using a TOPO TA Cloning Kit.
Plaque assay
Plaque assays were performed using SpIm cells for HycuMNPV, BM-N cells for BmNPV and Ld652Y cells for OpMNPV and LdMNPV, as described previously35. Briefly, monolayer cultures of these cells (0.5 × 106 cells) were prepared in 35 mm culture dishes (Falcon 3001) and inoculated with 200 μl of the virus suspensions to be titrated. After a 60 min adsorption period, the viral inoculum was replaced with 3 ml of 0.75% SeaPlaque agarose (FMC) in the medium appropriate for each cell line. The cultures were incubated at 28 °C for 5–6 days and formed plaques were counted under a microscope.
Transfection
The transfection of SpIm and Tn368 cells with expression plasmids and bacmids was performed using Lipofectin (Invitrogen) and FuGENE (Promega), respectively, as described previously39, 43.
References
Herniou, E. A. et al. In Virus taxonomy: classification and nomenclature of viruses, ninth report of the International Committee on Taxonomy of Viruses (eds King, A. M. Q., Adams, M. J., Carstens, E. B. & Lefkowitz, E. J.) 163–173 (Elsevier Academic Press, 2012).
Granados, R. R. & Williams, K. A. In vivo infection and replication of baculoviruses. In The biology of baculoviruses, Vol. 1. (eds Granados, R. R. & Federici, B. A.) 89–108 (CRC Press, 1986).
Harrison, R. L. & Bonning, B. C. The nucleopolyhedroviruses of Rachiplusia ou and Anagrapha falcifera are isolates of the same virus. J. Gen. Virol. 80, 2793–2798 (1999).
Hostetter, D. L. & Puttler, B. A new broad host spectrum nuclear polyhedrosis virus isolated from a celery looper, Anagrapha falcifera (Kirby), (Lepidoptera: Noctuidae). Environ. Entomol. 20, 1480–1488 (1991).
McIntosh, A. H. In vitro infectivity of a clonal isolate of Syngrapha falcifera (celery looper) multiple nuclear polyhedrosis virus. J. Invertebr. Pathol. 57, 441–442 (1991).
Thiem, S. M. Prospects for altering host range for baculovirus bioinsecticides. Curr. Opin. Biotechnol. 8, 317–322 (1997).
Thiem, S. M. Baculovirus genes affecting host function. In Vitro Cell. Dev. Biol. – Animal. 45, 111–126 (2009).
Thiem, S. M. & Cheng, X.-W. Baculovirus host-range. Virol. Sin. 24, 436–457 (2009).
Clem, R. J., Fechheimer, M. & Miller, L. K. Preventation of apoptosis by a baculovirus gene during infection of insect cells. Science. 254, 1388–1390 (1991).
Hershberger, P. A., Dickson, J. A. & Friesen, P. D. Site-specific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosis virus: Cell line specific effects on virus replication. J. Virol. 66, 5525–5533 (1992).
Croizier, G., Croizier, L., Argaud, O. & Poudevigne, D. Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene. Proc. Natl. Acad. Sci. USA 91, 48–52 (1994).
Kamita, S. G. & Maeda, S. Inhibition of Bombyx moni nuclear polyhedrosis virus (NPV) replication by the putative DNA helicase gene of Autographa califomica NPV. J. Virol. 67, 6239–6245 (1993).
Du, X. & Thiem, S. M. Characterization of host range factor 1 (hrf-1) expression in Lymantria dispar M nucleopolyhedrovirus- and recombinant Autographa californica M nucleopolyhedrovirus-infected IPLB-Ld652Y cells. Virology. 227, 420–430 (1997).
Lu, A. & Miller, L. K. Differential requirements for baculovirus late expression factor genes in two cell lines. J. Virol. 69, 6265–6272 (1995).
Lu, A. & Miller, L. K. Species-specific effects of the hcf-1 gene on baculovirus virulence. J. Virol. 70, 5123–5130 (1996).
Thiem, S. M., Du, X., Quentin, M. E. & Berner, M. M. Identification of a baculovirus gene that promotes Autographa californica nuclear polyhedrosis virus replication in a nonpermissive insect cell line. J. Virol. 70, 2221–2229 (1996).
Hefferon, K. L. Characterization of HCF-1, a determinant of Autographa californica multiple nucleopolyhedrovirus host specificity. Insect Mol. Biol. 12, 651–658 (2003).
Wilson, J. A. et al. Expression and mutational analysis of Autographa californica nucleopolyhedrovirus HCF-1: Functional requirements for cysteine residues. J. Virol. 79, 13900–13914 (2005).
Ishikawa, H., Ikeda, M., Felipe Alves, C. A., Thiem, S. M. & Kobayashi, M. Host range factor 1 from Lymantria dispar nucleopolyhedrovirus (NPV) is an essential viral factor required for productive infection of NPVs in IPLB-Ld652Y cells derived from L. dispar. J. Virol. 78, 12703–12708 (2004).
Ishikawa, H., Ogasawara, T., Ikeda, M. & Kobayashi, M. A recombinant Bombyx mori nucleopolyhedrovirus possessing hrf-1 gene replicates in nonpermissive Lymantria dispar IPLB-Ld652Y cell line. J. Insect Biotechnol. Sericol. 75, 31–38 (2006).
Herniou, E. A., Olszewski, J. A., Cory, J. S. & O’Reilly, D. R. The genome sequence and evolution of baculoviruses. Annu. Rev. Entomol. 48, 211–234 (2003).
Ikeda, M., Reimbold, E. A. & Thiem, S. M. Functional analysis of the baculovirus host range gene, hrf-1. Virology. 332, 602–613 (2005).
Bradford, M. B., Blissard, G. W. & Rohrmann, G. F. Characterization of the infection cycle of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus in Lymantria dispar cells. J. Gen. Virol. 71, 2841–2846 (1990).
Krejmer, M., Skrzecz, I., Wasag, B., Szewczyk, B. & Rabalski, L. The genome of Dasychira pudibunda nucleopolyhedrovirus (DapuNPV) reveals novel genetic connection between baculoviruses infecting moths of the Lymantriidae family. BMC Genomics. 16, 759 (2015).
Cohen, D. P. A., Marek, M., Davies, B. G., Vlak, J. M. & van Oers, M. M. Encyclopedia of Autographa californica nucleopolyhedrovirus genes. Virol. Sin. 24, 359–414 (2009).
Rohrmann, G. F. Baculovirus molecular biology, third edition. (National Center for Biotechnology Information (US), http://www.ncbi.nlm.nih.gov/books/NBK114593/ (2013)).
Wang, Y.-S. et al. Genome of Thysanoplusia orichalcea multiple nucleopolyhedrovirus lacks the superoxide dismutase gene. J. Virol. 86, 11948–11949 (2012).
Hink, W. F. Established insect cell line from the cabbage looper. Trichoplusia ni. Nature. 226, 466–467 (1970).
Mitsuhashi, J. & Inoue, H. Obtainment of a continuous cell line from the larval fat bodies of the mulberry tiger moth, Spilosoma imparilis (Lepidoptera: Arctiidae). Appl. Entomol. Zool. 23, 488–490 (1988).
Mitsuhashi, J. & Maramorosch, K. Leafhopper tissue culture: embryonic, nymphal, and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22, 435–460 (1964).
Nagamine, T., Shimomura, M., Sugimori, H. & Kobayashi, M. Titration of Bombyx mori (Lepidoptera: Bombycidae) nuclear polyhedrosis virus in a Bombyx mori cell line. Appl. Entomol. Zool. 24, 235–237 (1989).
Kamiya, K. et al. Genotypic variation of a wild isolate of Hyphantria cunea nucleopolyhedrovirus. J. Insect Biotechnol. Sericol. 72, 57–64 (2003).
Slavicek, J. M., Mercer, M. J., Kelly, M. E. & Hayes-Plazolles, N. Isolation of baculovirus variant that exhibits enhanced polyhedra production stability during serial passage in cell culture. J. Invertebr. Pathol. 67, 153–160 (1996).
Leisy, D. J., Rohrmann, G. F., Nesson, M. & Beaudreau, G. S. Nucleotide sequencing and transcriptional mapping of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus p10 gene. Virology. 153, 157–167 (1986).
Shirata, N. et al. Replication of nucleopolyhedroviruses of Autographa californica (Lepidoptera: Noctuidae), Bombyx mori (Lepidoptera: Bombycidae), Hyphantria cunea (Lepidoptera: Arctiidae), and Spodoptera exigua (Lepidoptera: Noctuidae) in four lepidopteran cell lines. Appl. Entomol. Zool. 34, 507–516 (1999).
Hamajima, R., Kobayashi, M. & Ikeda, M. p143-mediated rRNA degradation in AcMNPV-infected BM-N cells is not associated with viral DNA replication. J. Insect Biotechnol. Sericol. 83, 19–23 (2014).
Ikeda, M., Yanagimoto, K. & Kobayashi, M. Identification and functional analysis of Hyphantria cunea nucleopolyhedrovirus iap genes. Virology. 321, 359–371 (2004).
Suganuma, I. et al. Cloning and characterization of a dronc homologue in the silkworm, Bombyx mori. Insect Biochem. Mol. Biol. 41, 909–921 (2011).
Ikeda, M., Yamada, H., Ito, H. & Kobayashi, M. Baculovirus IAP1 induces caspase-dependent apoptosis in insect cells. J. Gen. Virol. 92, 2654–2663 (2011).
Yamada, H., Shibuya, M., Kobayashi, M. & Ikeda, M. Baculovirus Lymantria dispar multiple nucleopolyhedrovirus IAP2 and IAP3 do not suppress apoptosis, but trigger apoptosis of insect cells in a transient expression assay. Virus Genes. 43, 370–379 (2012).
Shirata, N., Ikeda, M. & Kobayashi, M. Identification of a Hyphantria cunea nucleopolyhedrovirus (NPV) gene that is involved in global protein synthesis shutdown and restricted Bombyx mori NPV multiplication in a B. mori cell line. Virology. 398, 149–157 (2010).
Katou, Y., Ikeda, M. & Kobayashi, M. Abortive replication of Bombyx mori nucleopolyhedrovirus in Sf9 and High Five cells: Defective nuclear transport of the virions. Virology. 347, 455–465 (2006).
Katou, Y., Ikeda, M. & Kobayashi, M. Characterization of Bombyx mori nucleopolyhedrovirus infection of Spodoptera frugiperda cells. J. Insect Biotechnol. Sericol. 70, 137–147 (2001).
Acknowledgements
We thank Kathleen L. Hefferon for kindly providing the plasmid pHSHAHishcf-1VI+. This work was supported by JSPS KAKENHI Grant Numbers JP26292173, JP16H06866 and JP15J02649 from the Japan Society for the Promotion of Science (JSPS). R.H. was supported by the Research Fellowship for Young Scientists from the JSPS and the Program for Leading Graduate Schools “Integrative Graduate Education and Research in Green Sciences” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.
Author information
Authors and Affiliations
Contributions
A.T., R.H., M.K., H.Y. and M.I. conceived and designed the research. A.T., R.H., M.T., T.K. and Y.N. performed the experiments. A.T., R.H., M.K., H.Y. and M.I. analyzed data and wrote the manuscript.
Corresponding author
Ethics declarations
Competing Interests
The authors declare that they have no competing interests.
Additional information
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Tachibana, A., Hamajima, R., Tomizaki, M. et al. HCF-1 encoded by baculovirus AcMNPV is required for productive nucleopolyhedrovirus infection of non-permissive Tn368 cells. Sci Rep 7, 3807 (2017). https://doi.org/10.1038/s41598-017-03710-z
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-017-03710-z
- Springer Nature Limited
This article is cited by
-
NISES-AnPe-428 cell line derived from the Chinese oak silkworm Antheraea pernyi is permissive for multiple nucleopolyhedrovirus species from insects of four different families
Cytotechnology (2021)
-
Genomic sequencing of Troides aeacus nucleopolyhedrovirus (TraeNPV) from golden birdwing larvae (Troides aeacus formosanus) to reveal defective Autographa californica NPV genomic features
BMC Genomics (2019)