Abstract
Mitochondrial quality control (MQC) protects against potentially damaging events, such as excessive generation of mitochondrial reactive oxygen species (mtROS). We investigated the contribution of the two major MQC processes, namely, mitophagy and Mieap-induced accumulation of lysosomes within mitochondria (MALM), to the response to hypoxia of two human gastric cancer (GC) cell lines. We found that hypoxia increased mtROS generation and cell invasion in 58As9, but not in MKN45, although the transcription factor hypoxia-inducible factor 1α was induced in both cell lines. Colocalisation of lysosomes with mitochondria was found only in hypoxic MKN45 cells, suggesting that hypoxia-induced MQC functions normally in MKN45 but may be impaired in 58As9. Hypoxia did not lead to decreased mitochondrial mass or DNA or altered appearance of autophagosomes, as judged by electron microscopy, suggesting that mitophagy was not induced in either cell line. However, western blot analysis revealed the presence of the MALM-associated proteins Mieap, BNIP3 and BNIP3L, and the lysosomal protein cathepsin D in the mitochondrial fraction of MKN45 cells under hypoxia. Finally, Mieap knockdown in MKN45 cells resulted in increased mtROS accumulation and cell invasion under hypoxia. Our results suggest that hypoxia-induced MALM suppresses GC cell invasion by preventing mtROS generation.
Similar content being viewed by others
Introduction
Mitochondria play crucial roles in maintaining cellular homeostasis by regulating diverse processes such as energy production, cell signalling and apoptosis1,2. These organelles are also a major source of intracellular reactive oxygen species (ROS), which include highly reactive free oxygen radicals, such as the superoxide anion (O2·−) and the hydroxyl radical (OH·), as well as stable nonradical oxidants such as hydrogen peroxide (H2O2)3,4. ROS are commonly produced as by-products of oxidative phosphorylation1,2, but excessive ROS generation in the mitochondria (mtROS) can lead to oxidative damage to proteins, lipids and DNA, sometimes resulting in apoptosis1,2. In addition, ROS accumulation is known to contribute to various diseases, such as degenerative disorders and cancer2,5. Recent reports suggest that elevated levels of mtROS promote cancer cell invasion and metastasis via the activation of several major signalling pathways and transcription factors6,7,8.
Hypoxia is a common characteristic of the microenvironment of solid tumours and leads to increased generation of mtROS by cancer cells9,10. In response to hypoxia, levels of the transcription factor hypoxia-inducible factor (HIF)-1 increase, leading to the transcription of genes that regulate oxygen homeostasis and promote the survival of cancer cells11,12,13,14,15,16. HIF-1 is a heterodimer composed of a constitutively expressed HIF-1β subunit and O2-regulated HIF-1α. Under normoxic conditions, HIF-1α is maintained at low levels via hydroxylation by the O2 sensor prolyl hydroxylase 2 (PHD2), which triggers its degradation via the ubiquitin–proteasome pathway11,12,16. Under hypoxic conditions, however, the low O2 tension inactivates PHD2 and HIF-1α is thus stabilised11,12. Elevation of mtROS also stabilises HIF-1α since PHD2 is inactivated by the oxidation of Fe(II) in its catalytic centre17,18,19. Thus, mtROS regulation of HIF-1α is a pivotal mechanism underlying cancer progression under hypoxia19. Indeed, a notable study by Ishikawa et al.20 demonstrated the importance of mtROS in tumour cell metastasis in mouse. The authors replaced endogenous mitochondria from a poorly metastatic tumour cell line with those from a highly metastatic cell using cybrid technology20. Impairment of the electron transport chain by mutation of mtDNA genes in these cells induced excessive ROS production and increased metastatic ability20, suggesting that mitochondrial integrity and activity are important determinants of the metastatic ability of cancer cells.
Mitochondrial quality control (MQC) pathways are essential for monitoring and maintaining mitochondrial integrity under conditions of stress, in part by preventing excessive mtROS production21. Mitophagy, an organelle-specific form of autophagy, is a major mechanism of MQC activated in response to certain physiological stresses, such as hypoxia and nutrient deprivation22,23,24. During mitophagy, the entire mitochondria are engulfed in double-membraned autophagosomes and ultimately degraded in lysosomes22,23,24. Recent studies demonstrated that hypoxia-induced mitophagy requires HIF-1α-dependent expression of BNIP3, a BCL2 family member25,26. Importantly, defects in mitophagy arising from the loss of BNIP3 are known to promote metastasis in mammary tumours27. In addition, Liu et al.28 reported that the mitochondrial membrane protein FUNDC1 plays a critical role in hypoxia-induced mitophagy through a mechanism distinct from that played by BNIP328.
Miyamoto et al.29 described another MQC mechanism in which the p53 target gene mitochondria-eating protein (Mieap) induced the accumulation of lysosomal proteins within the mitochondria of colorectal cancer cell lines in response to γ-irradiation, resulting in the elimination of oxidised mitochondrial proteins, repair of the unhealthy mitochondria and improvement of mitochondrial respiration29,30. In this process, termed MALM (Mieap-induced accumulation of lysosomes within mitochondria), interaction of Mieap with BNIP3 or BNIP3L facilitates the translocation of lysosomal proteins into the mitochondria by inducing pore formation in the mitochondrial double membrane30,31,32. However, it is not known whether MALM is induced in cancer cells by hypoxia or whether loss of MALM accelerates cancer progression under hypoxic conditions. We previously reported that impairment of hypoxia-induced MQC is positively correlated with hypoxia-induced invasion in gastric cancer (GC) cells. However, we did not investigate the MQC mechanism underlying this33.
In the present study, we sought to identify the mechanism of hypoxia-induced MQC using two GC cell lines (58As9 and MKN45) that differ in their ability to invade, an initial step towards cancer metastasis. We investigated the effects of hypoxia on mtROS generation and invasion and determined the contribution of mitophagy and MALM to hypoxia-induced MQC in these cells.
Results
Effects of hypoxia on invasion and ROS generation by GC cell lines
To determine how hypoxia affects the invasiveness of the two GC cell lines 58As9 and MNK45, we performed Transwell invasion assays by incubating cells for 48 h under conditions of normoxia (5% CO2 in air) or hypoxia (1% O2 and 5% CO2 in N2). As shown in Fig. 1, hypoxia significantly increased the number of invaded 58As9 cells, whereas MKN45 cells were unaffected (Fig. 1a,b). Measurement of total intracellular ROS levels using a 2′,7′-dichlorofluorescein diacetate-based flow cytometric assay indicated that exposure to hypoxia for up to 48 h significantly increased ROS production in 58As9 cells, while ROS levels in MKN45 cells were only marginally increased (Fig. 1c). These findings indicate that 58As9 cells respond to hypoxia by increasing mtROS production and cell invasion, whereas MKN45 cells are relatively unaffected by hypoxia.
mtROS generation and MQC status in GC cells exposed to hypoxia
To determine whether the increase in total intracellular ROS resulted from mtROS generation, we labelled the cells with fluorescent markers of mitochondria (MitoTracker) and mtROS (MitoSOX Red) and examined their colocalisation by microscopy. Consistent with the results of the flow cytometric assay, we observed the colocalisation of MitoSOX Red and MitoTracker fluorescence in several 58As9 cells, but not MKN45 cells, after incubation for 48 h under hypoxia (Fig. 2a). MitoSOX Red fluorescence was not increased in either cell line incubated under normoxia (data not shown).
We next investigated whether the difference in responses to hypoxia between the two cell types might be due to differences in their MQC activity. To this end, cells were exposed to hypoxia for 48 h and the colocalisation of mitochondria and lysosomes was assessed by double staining with MitoTracker and LysoTracker, a fluorescent lysosome marker. Notably, dotted colocalisation of the fluorescent signals was observed in several MKN45 cells, but not in 58As9 cells (Fig. 2b). Under normoxia, neither cell line showed overlapping MitoTracker and LysoTracker signals (data not shown). Taken together, these data indicate that hypoxia induces mtROS generation in 58As9 cells but not in MKN45 cells, whereas it induces the formation of mitochondrial lysosomes in MKN45 cells but not in 58As9 cells; thus, MKN45 cells appear to mount a more vigorous MQC response than 58As9 cells.
Effect of N-acetyl-l-cysteine (NAC) treatment on mtROS generation and invasion in 58As9 cells
To determine whether the production of mtROS by 58As9 cells directly regulates hypoxia-induced invasion, we treated the cells with NAC, a ROS scavenger, and measured invasion under normoxic and hypoxic conditions. First, we confirmed that NAC effectively reduced mtROS generation using the fluorescence assay. Indeed, while exposure to hypoxia in the absence of NAC significantly increased mtROS generation, the response was inhibited by NAC treatment (Fig. 3a,b). Interestingly, NAC had the same effect on 58As9 cell invasion measured after incubation for 48 h under hypoxia or normoxia (Fig. 3c,d), suggesting that elevated mtROS levels were required for invasion. We also observed that cell proliferation was significantly decreased in NAC-treated 58As9 cells compared with that in control, untreated cells; however, this was not accompanied by an increase in cell death (Supplementary Fig. S1). In addition, we assessed the effects of NAC on HIF-1α protein levels by western blot analysis. Exposure to hypoxia for 48 h induced HIF-1α expression in untreated 58As9 cells, but this was strongly inhibited by NAC treatment (Fig. 3e). Collectively, these findings suggest that the inhibition of mtROS generation by NAC treatment reduces cell invasion, cell proliferation and HIF-1α expression in 58As9 cells under hypoxia.
Effect of the lysosomal inhibitor chloroquine (CQ) on hypoxia-induced MQC in MKN45 cells
Because we observed that MKN45 cells are protected against the effects of hypoxia on mtROS and show hypoxia-induced colocalisation of lysosomes and mitochondria, we next assessed the effects of inhibiting lysosomal function in these cells by incubation with CQ, which inhibits lysosome acidification. CQ-treated MKN45 cells under hypoxia for 48 h showed increased mtROS production compared with untreated cells (Fig. 4a), whereas the untreated cells were unaffected by hypoxia (Fig. 4b). The same pattern of response to hypoxia and CQ treatment was observed in Transwell invasion assays (Fig. 4c,d), indicating that functioning lysosomes are necessary for MKN45 cells to prevent hypoxia-induced increases in mtROS and cell invasion. CQ treatment had no effects on either the proliferation or the survival of MKN45 cells under normoxia or hypoxia (Supplementary Fig. S2) or on hypoxia-induced expression of HIF-1α (Fig. 4e). Collectively, these findings suggest that the inhibition of lysosome function by CQ treatment elevates mtROS generation and promotes the invasiveness of hypoxic MKN45 cells.
Assessment of hypoxia-induced mitophagy in GC cells
We next determined whether mitophagy was activated in hypoxia-exposed 58As9 or MKN45 cells. Mitophagy eliminates damaged mitochondria via engulfment in autophagosomes, leading to reductions in mtDNA copy number and mitochondrial mass. The results showed that the mean copy number of mtDNA was significantly higher in 58As9 cells incubated for 48 h under hypoxia than for those under normoxia, whereas the copy number did not differ between normoxic and hypoxic MKN45 cells (Fig. 5a). We also measured the mitochondrial mass in GC cells by staining with nonyl acridine orange (NAO), a metachromatic fluorescent dye that binds to mitochondria regardless of their membrane potential. As shown by the flow cytometry profiles, mitochondrial mass was slightly elevated in both cell lines after 48 h of incubation under hypoxic compared with normoxic conditions, and there was no apparent difference between the cell lines (Fig. 5b). In both cell lines, the total amount of NAO was significantly increased under hypoxia, compared with that under normoxia (Fig. 5c). Next, we analysed hypoxia-induced changes in mitochondrial morphology by electron microscopy. In both cell lines cultured under normoxia, the mitochondrial cristae appeared as dense and regularly folded structures; however, cristae were sparser and more irregularly shaped in 58As9 cells under hypoxia for 48 h, whereas those in MKN45 cells were relatively unaffected (Fig. 5d). In addition, we did not observe mitochondrial engulfment by autophagosomes in either cell type under normoxia or hypoxia (Fig. 5d). Taken together, these findings indicate that mitophagy may not be induced by hypoxia in either GC cell line.
Investigation of Mieap expression and hypoxia-induced MALM
We next analysed the expression of several MQC-related genes involved in mitophagy (FUNDC1, BNIP3 and BNIP3L) and MALM (Mieap). As shown in Fig. 6a, each of FUNDC1, BNIP3 and BNIP3L mRNA was expressed in both 58As9 and MKN45 cells, and the expression of BNIP3 and BNIP3L mRNA was strongly induced by hypoxia. In contrast, Mieap mRNA was detectable only in MKN45 cells, and there was no apparent difference between the levels detected under normoxia or hypoxia (Fig. 6a). Consistent with this, western blot analysis revealed that Mieap protein was expressed in MKN45 cells, but not in 58As9 cells, exposed to normoxia or hypoxia for 12–48 h (Fig. 6b). Moreover, analysis of fractionated cell lysates indicated that, although Mieap protein was predominantly cytosolic in MKN45 cells, hypoxia induced its appearance in the mitochondrial fraction of MKN45 cells, but not that of 58As9 cells (Fig. 6c). Consistent with the qRT-PCR analyses, expression of the HIF-1α target genes BNIP3 and BNIP3L was induced by hypoxia in the mitochondrial fractions of both GC cell lines (Fig. 6c). To assess the mechanism of MALM, we examined the subcellular localisation of lysosomes and the function of mitochondria in hypoxia-exposed cells in more detail. As measured by western blot analysis, expression of the lysosomal protein cathepsin D was not detected in the cytosolic or mitochondrial fractions of 58As9 cells incubated under normoxia or hypoxia for 24 h (Fig. 6d). In contrast, the protein was detectable in the cytosol but not the mitochondria of MKN45 cells under normoxia, but the expression was increased in both compartments after exposure to hypoxia (Fig. 6d), thus confirming the localisation of lysosomes to the mitochondria only in hypoxic MKN45 cells. To assess the effects of hypoxia on mitochondrial function, we measured the mitochondrial membrane potential (MMP) and O2 consumption rate (OCR) in both cell lines. MMP was estimated by staining cells with tetramethylrhodamine (TMRM), a dye that accumulates in mitochondria in an MMP-dependent manner. We found that exposure to hypoxia for 72 h induced a stronger decrease in the MMP in MKN45 cells than in 58As9 cells (Fig. 6e). OCR was measured using a fluorescent assay under normoxia or hypoxia-mimicking conditions (CoCl2), and the OCR ratio under hypoxia compared with normoxia was estimated. This analysis indicated that the OCR ratio was significantly higher in MKN45 than in 58As9 cells (Fig. 6f). Collectively, these findings suggest that MALM is induced in MKN45 cells under hypoxia, while the mechanism is impaired in 58As9 cells.
Analysis of cell invasion and mtROS accumulation in Mieap-knockdown MKN45 cells
Next, we determined the functional effects of disrupting MALM-mediated MQC by establishing MKN45 cells with the stable expression of Mieap-specific shRNA or scrambled shRNA (KD or SC cells, respectively). At this point, two clones (clones 1 and 2) were actually established by transfection using different sequences for Mieap-specific shRNA. Clone 2, exhibiting stronger inhibition of Mieap expression than clone 1, was designated as KD cells, and used in further study (Supplementary Fig. S9). Mieap expression was confirmed by western blot analysis to be strongly reduced in KD cells compared with that in SC cells (Fig. 7a). Intriguingly, Mieap KD significantly increased MKN45 cell invasion under hypoxia compared with that under normoxia, whereas invasion by control SC cells was unaffected by hypoxia (Fig. 7b,c). Consistent with this, mtROS levels were elevated by hypoxia in KD cells, but not in SC cells (Fig. 7d,e). These findings suggest that MALM requires Mieap expression and plays a pivotal role in preventing the hypoxia-induced increase in mtROS generation and cell invasion in MKN45 cells.
Investigation of MALM activity in MKN45 cells with Mieap knockdown
Finally, we analysed the effects of Mieap KD on MALM at the molecular level in MKN45 cells. Using the fluorescence MitoTracker/LysoTracker assay, we observed that hypoxia induced the colocalisation of lysosomes and mitochondria in hypoxic SC cells, while this was largely abolished in KD cells (Fig. 8a). Electron microscopy revealed that the morphological features of mitochondria in SC cells exposed to normoxia and hypoxia were similar; however, the mitochondrial cristae were sparser and more irregularly shaped under hypoxia than under normoxia in KD cells (Fig. 8b). Moreover, western blot analysis revealed weak Mieap expression in the mitochondrial fraction of SC cells, but not that of KD cells, under hypoxia (Fig. 8c); similarly, cathepsin D was expressed in the mitochondrial fraction only of SC cells under hypoxia (Fig. 8c). Lastly, we analysed the OCR in SC and KD cells under normoxic and hypoxia-mimicking conditions. As shown in Fig. 8d, the OCR ratio in KD cells was significantly lower than that in SC cells. In conclusion, these findings suggest that Mieap KD inhibits significant features of MALM in MKN45 cells under hypoxia.
Discussion
Tumour hypoxia is known to induce mtROS accumulation and to promote cancer cell survival and progression3,4. The goal of this study was to elucidate the mechanism by which mtROS increase the invasive capacity of GC cells under hypoxic conditions. We confirmed that exposure to hypoxia induced mtROS accumulation and invasion in 58As9 cells, but not in the poorly invasive MKN45 cell line. During mitophagy and MALM, mitochondria are targeted to lysosomes21,22,30. We found that mitochondria colocalised with lysosomes only in hypoxic MKN45 cells, indicating that the MQC system may be impaired in 58As9 cells.
To assess whether mtROS generation directly regulates hypoxia-induced cancer invasion, we treated the cells with the ROS scavenger NAC. This approach revealed a critical role for mtROS in the HIF-1α expression, invasion and proliferation of 58As9 cells under hypoxia. Previous studies have shown that HIF-1α upregulates genes involved in cancer invasion under hypoxia14,15,16, and ROS generation is known to increase HIF-1α stability via PHD2 inactivation3,4. One interpretation of our data is that the scavenging of mtROS by NAC decreased HIF-1α stability, thereby suppressing the invasion and proliferation of 58As9 cells under hypoxia. We also demonstrated that the inhibition of lysosomal function by CQ increased mtROS generation and invasion by hypoxic MKN45 cells, but did not affect HIF-1α expression, cell proliferation or cell death. These results indicate that hypoxia-induced mtROS promote the invasiveness of MKN45 cells and that lysosomal function is required for hypoxia-induced MQC in these cells.
We attempted to elucidate the mechanism of hypoxia-induced MQC in GC cells by exploring various aspects of mitophagy and MALM. Mitophagy involves engulfment and elimination of damaged mitochondria via autophagosomes, leading to reductions in mtDNA copy number and mitochondrial mass21,22.
However, we found that neither cell line showed a decrease in mean copy number of mtDNA or mitochondrial mass under hypoxia compared with that under normoxia. Unexpectedly, in 58As9 cells, the copy number of mtDNA was significantly increased under hypoxia compared with that under normoxia. Replication or repair of mtDNA may be promoted by mtROS-mediated damage to DNA in hypoxic 58As9 cells. In addition, total mitochondrial mass was also increased by hypoxia in both cell lines, suggesting that mitochondrial neogenesis may be enhanced by hypoxia. However, the mechanisms by which the copy number of mtDNA in 58As9 and mitochondrial mass in both cell lines were increased under hypoxia are currently unclear. Moreover, morphological changes in mitochondrial cristae were found in hypoxic 58As9 cells, but not in MKN45 cells, suggesting that mtROS generation may have destroyed the structure of cristae in hypoxic 58As9 cells. Notably, autophagosomes engulfing mitochondria were not observed in either cell line by electron microscopy21,22,28. Taken together, these findings suggest that hypoxia may not induce mitophagy in MKN45 cells.
We examined the mechanism of hypoxia-induced MQC in more detail by analysing the expression of genes related to mitophagy and MALM. Interestingly, BNIP3 and BNIP3L mRNA was detected in 58As9 and MKN45 cells and was elevated in response to hypoxia in both cell lines, whereas Mieap was expressed only in MKN45 cells and was not significantly changed under conditions of hypoxia compared with normoxia. Previous work showed that Mieap forms a complex with BNIP3 or BNIP3L on the mitochondrial outer membrane in γ-irradiated colon cancer cell lines30,31. During MALM, the interaction between Mieap and BNIP3 or BNIP3L may open a pore between the outer and inner mitochondrial membranes, thus enabling lysosomes to enter the mitochondria30,31. During this process, MMP is reduced without cell death31. In the present study, we found that Mieap was strongly expressed in the cytosol of MKN45 cells under normoxia and hypoxia, but it was detected in the mitochondrial fraction only under hypoxia. In addition, BNIP3 and BNIP3L were detected in the mitochondrial fraction of both cell lines under hypoxia, but not normoxia. These results imply that hypoxia may induce the expression of BNIP3 and BNIP3L on the mitochondrial outer membrane, leading to the recruitment of Mieap. The following findings from this study in combination suggest that MALM was operating in hypoxic MKN45 cells, but not in 58As9 cells: (i) cathepsin D was observed in the mitochondrial fraction of hypoxic MKN45 cells, but not in 58As9 cells; (ii) hypoxia induced a stronger reduction in the MMP in MKN45 cells than in 58As9 cells; and (iii) MKN45 cells displayed a higher OCR ratio (hypoxia/normoxia) than did 58As9 cells. Furthermore, cathepsin D may be transported into the mitochondria through mitochondrial membrane pores formed by Mieap–BNIP3 or Mieap–BNIP3L complexes. Cathepsin D may then digest oxidised proteins in the mitochondria, thereby contributing to the restoration of mitochondrial respiration, as previously reported to occur during MALM31.
The effects of Mieap KD identified in this study confirmed the involvement of MALM in the MQC in MKN45 cells. Compared with those in control SC cells, KD of Mieap resulted in significant elevations of mtROS generation and cell invasion under hypoxia; induced morphological changes in the mitochondrial cristae under hypoxia; inhibited cathepsin D protein expression in the mitochondrial fraction under hypoxia; and significantly decreased the OCR ratio (hypoxia/normoxia). Taken together, these results indicate that Mieap is required for hypoxia-induced MALM in MKN45 cells, which may regulate mtROS generation, leading to the suppression of cell invasion. However, further investigation will be necessary to confirm that lysosomal proteins are transported into the mitochondria (e.g., by proteinase K digestion analysis)31, and that complexes are formed between Mieap and BNIP3 or BNIP3L (e.g., by coimmunoprecipitation)31. Notably, Mieap expression is reportedly reduced by the mutation of TP53 or methylation of its promoter in colon cancer cells and tissues29,34. We found that Mieap mRNA was expressed in five of nine GC cell lines tested (including 58As9 and MKN45; data not shown). Previous studies reported that wild-type p53 was expressed in MKN45, while the status of TP53 in 58As9 has not been investigated35,36. Therefore, we analysed p53 expression in MKN45 and 58As9 cells by western blotting (Supplementary Fig. S10). The results showed that both cells expressed p53 under normoxia and hypoxia (Supplementary Fig. S10). Interestingly, positive p53 signal less than 53kd was observed in 58As9, compared with the molecular weight of p53 in MKN45 (Supplementary Fig. S10). The p53 antibody used in this study recognizes the N-terminal region of this protein. Therefore, some frameshift mutation in TP53, whereby a C-terminus-truncated p53 isoform is produced, may occur in 58As9 cells, as previously reported in high-grade serous ovarian cancer37. The TP53 mutation may result in the loss of function of p53 in 58As9. Furthermore, treatment with the DNA methyltransferase inhibitor 5-aza-dC did not increase Mieap mRNA expression in 58As9 cells (Supplementary Fig. S11). Based on these findings, we hypothesize that TP53 mutation, but not DNA methylation of the Mieap gene promoter, may suppress Mieap expression in 58As9. However, further analysis of the TP53 mutation or Mieap promoter methylation may be necessary to clarify the mechanism of Mieap loss in 58As9 cells.
In conclusion, we report the first demonstration that MALM, and not mitophagy, is induced in GC cells under hypoxic conditions and demonstrate that hypoxia-induced MALM requires Mieap expression. Finally, this noncanonical MQC mechanism may also control mtROS production and suppress cell invasion in GC cells in hypoxic environments.
Methods
Cell culture and reagents
The GC cell line 58As9 was kindly provided by K. Yanagihara (National Cancer Center Hospital, Kashiwa, Japan). 58As9 was established from parental HSC-58 (scirrhous gastric carcinoma-derived cell line), and was reported to be associated with high rates of fatal cancerous peritonitis and ascites in a mouse orthotopic implantation model38. Another GC cell line, MKN45 (poorly differentiated adenocarcinoma), was purchased from the RIKEN Cell Bank (Tsukuba, Japan). The cell lines were grown in complete culture medium [RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS; Biowest, Nuaillé, France) and 100 µg/ml kanamycin (Meiji, Tokyo, Japan)] at 37 °C in a humidified atmosphere. For the experiments, cells were cultured under normoxic (5% CO2 in air) and hypoxic conditions (1% O2, 5% CO2 in N2). Chloroquine diphosphate (CQ), N-acetyl-l-cysteine (NAC) and 5-aza-2-deoxycytidine (5-aza-dC) were purchased from Sigma-Aldrich and used at final concentrations of 20 mM, 10 and 5 µM, respectively.
In vitro invasion assays
GC cells were resuspended in serum-free RPMI-1640 culture medium (1 × 105 cells/200 µl) and seeded into the upper chambers of BioCoat Matrigel Invasion Chambers (354480; Corning) in 24-well plates. Aliquots of 500 µl of the supernatant from cultures of the MRC5 lung cancer cell line were placed in the bottom chambers. Plates were incubated for 48 h in normoxic or hypoxic conditions, and then noninvading cells on the upper side of the filter were gently removed with a cotton swab. The invaded cells on the lower side of the filter were fixed in 4% paraformaldehyde for 15 min and then stained with a 0.1% crystal violet solution for 15 min. Using a light microscope, cells in three random fields were visualised and enumerated with ImageJ software. All experiments were performed in triplicate.
Knockdown of Mieap
pKLO.1-hU6 Pur plasmids encoding Mieap-specific shRNAs [TRCN0000141572 (clone 1) and TRCN0000142712 (clone 2)] or a control scrambled shRNA (SHC002) were purchased from Sigma-Aldrich. Cells were transfected with the plasmids using Lipofectamine 3000 (Thermo Fisher Scientific, Tokyo, Japan), in accordance with the manufacturer’s instructions. Cells stably expressing the Mieap shRNA or control shRNA (referred to as SC) were selected using puromycin.
Western blot analysis
Whole-cell lysates were prepared by the resuspension of cells in lysis buffer [150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 2 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 2% SDS, 28 µM PMSF and a protease inhibitor cocktail mix (Roche, Mannheim, Germany)]. The lysates were sonicated for 30 s and the supernatants were then removed. For experiments analysing fractionated lysates, a Mitochondria/Cytosol Fractionation Kit (BioVision, Milpitas, CA, USA) was used, in accordance with the manufacturer’s instructions. Western blot analysis was performed as previously described39. In brief, aliquots containing 20 µg of protein (or 10 µg of cytosol/mitochondrial fractionated protein) were separated on 5–20% Bis-Tris gels (Intertechno, Tokyo, Japan) and transferred to Hybond-ECL membranes (GE Healthcare, Little Chalfont, UK). Membranes were blocked with 5% skim milk or 2% bovine serum albumin in Tris-buffered saline–0.01% Tween 20 for 30 min, and then incubated overnight at 4 °C with the following primary antibodies: anti-HIF-1α (1:1000, 610958; BD Biosciences), anti-Mieap (1:1000, HPA036854; Sigma-Aldrich), anti-TOM40 (1:100, sc365466; Santa Cruz Biotechnology), anti-TIM22 (1:1000, ab167423; Abcam), anti-cathepsin D (1:1000, 66534-1-Ig; Proteintech), anti-BNIP3 (1:1000, #13795; Cell Signaling Technology), anti-BNIP3L (1:1000, #12396; Cell Signaling Technology), anti-p53 (1:200, sc-126; Santa Cruz Biotechnology) and anti-β-actin (1:10,000, AC15; Sigma-Aldrich). Membranes were then washed and incubated with the corresponding secondary antibodies (SouthernBiotech, Birmingham, AL, USA) and the signal was developed using ECL Prime Western Blotting Detection Reagent (GE Healthcare). Images were acquired using a LAS-3000 Imaging System (Fujifilm, Tokyo, Japan).
Quantification of intracellular ROS levels by flow cytometry
Intracellular ROS levels were evaluated using a 2′,7′-dichlorofluorescein diacetate-based Total ROS Detection Kit (ENZO Life Sciences, Farmingdale, NY, USA), in accordance with the manufacturer’s instructions. In brief, at the end of the experiment, cells (1 × 105/sample) were washed and resuspended in ROS detection solution. Fluorescence was detected using a FACSCalibur flow cytometer (Becton-Dickinson, San Jose, CA, USA) using excitation and emission wavelengths of 488 and 545 nm, respectively. Data were analysed using FlowJo version 10.0 software (FlowJo, Ashland, OR, USA). All experiments were performed in triplicate. Data are displayed as the geometric mean fluorescence.
Quantitative RT-PCR
Total RNA was extracted from cells using Isogen II (Nippon Gene, Osaka, Japan), and aliquots of 1 µg per sample were reverse-transcribed using a ReverTra Ace kit (Toyobo). RT-qPCR was performed using the CFX Connect Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad), in accordance with the manufacturer’s protocol. The PCR program was performed using two StepAmp procedures, as described previously35. β-Actin mRNA served as an internal control. Primers for FUNDC1, BNIP3, BNIP3L, MIEAP and ACTB were as follows: FUNDC1 sense, 5′-GTAATGGGTGGCGTTACTGG-3′ and antisense, 5′-GCTTTGTTCGCTCGTTTCTT-3′; BNIP3 sense 5′-ACCCTCAGCATGAGGAACAC-3′ and antisense 5′-CAGCAAATGAGAGAGAGCAGCA-3′; BNIP3L sense 5′-GATGTGGAAATGCACACCAG-3′ and antisense 5′-TACCCAGTCCGCACTTTTCT-3′; MIEAP sense 5′-ATGATTGCAAATACCGCCGC-3′ and antisense 5′-CGACTTACAGATCGCACCGA-3′; and ACTB sense 5′-ACGCCTCTGGCCGTACCACT-3′ and antisense 5′-TAATGTCACGCACGATTCCC-3′.
Confocal laser microscopy of mitochondrial markers
Cells (3 × 105/sample) were seeded in 35-mm glass dishes and cultured in normoxic or hypoxic conditions with or without drugs as indicated. Cells were then stained with 100 nM MitoTracker Green FM (M7514; Invitrogen) to detect mitochondria; 5 µM MitoSOX Red (M36008; Invitrogen) to detect mtROS; or 50 nM LysoTrackerRed DND-99 (L7528; Invitrogen) to detect lysosomes. Images were acquired using an LSM-880 confocal laser microscope (Carl Zeiss). For each sample, the mean mtROS levels in three fields were calculated using ZEN software.
Assessment of mitochondrial DNA copy number
At the end of the experiment, total DNA was extracted from cells using a DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). The ratio of mitochondrial mtDNA to nuclear DNA (mtDNA:nuDNA) was determined by quantitative PCR (qPCR) using a Human Mitochondrial DNA (mtDNA) Monitoring Primer Set (TaKaRa, Kusatsu, Japan), in accordance with the manufacturer’s instructions. The kits included primers for ND1 and ND5 as the mtDNA targets and SLCO2B1 and SERPINA1 as the nuDNA targets. In brief, qPCR analysis was performed using the primer sets of ND1, ND5, SLCO2B1 and SERPINA1, and the threshold cycle (Ct) values were determined. Next, ΔCt1 (Ct value of SLCO2B1 − Ct value of ND1) and ΔCt2 (Ct value of SERPINA1 − Ct value of ND5) were calculated. Finally, the mean copy number of mtDNA was determined by calculating the mean value of 2ΔCt1 and 2ΔCt2.
Measurement of mitochondrial mass and mitochondrial membrane potential (MMP) by flow cytometry
Mitochondrial mass was measured by staining cells with 20 nM nonyl acridine orange (NAO) (Thermo Fisher Scientific) in phosphate-buffered saline (PBS)/5% FBS at 37 °C for 15 min. MMP was measured by staining cells with 250 nM tetramethylrhodamine methyl ester (TMRM; Thermo Fisher Scientific) at 37 °C for 30 min in complete medium. Both sets of cells were analysed using FACSCalibur with excitation and emission wavelengths of 488 and 575 nm, respectively.
Electron microscopy
Cells (3 × 105/sample) were seeded in 60-mm dishes and cultured in normoxic or hypoxic conditions for 48 h. The cells were fixed in 3% glutaraldehyde in PBS at 4 °C for 2 h, washed with PBS, post-fixed in 1% OsO4 buffered with PBS for 2 h, dehydrated in a graded series of ethanol and then embedded in Epon812. Ultrathin sections (90 nm) were placed on copper grids, double-stained with uranyl acetate and lead citrate, and then observed using a transmission electron microscope (JEM-1400Flash; JEOL, Akishima, Japan).
Measurement of oxygen consumption rate (OCR) under hypoxia-mimicking conditions with CoCl2
OCR was evaluated using a MitoXpress Xtra Oxygen Consumption Assay kit (Luxel Biosciences, Cork, Ireland). In brief, 5 × 104 cells were seeded in 96-well black plates and cultured under normoxia with or without cobalt(II) chloride hexahydrate (CoCl2) for 24 h. The medium was exchanged for fresh culture medium, MitoXpress Xtra reagent was added to each well and the wells were sealed with two drops of pre-warmed HS Mineral Oil. Fluorescent readings were started immediately using a fluorescence plate reader (CLARIOstar; BMG LABTECH). The OCR was estimated using a Dual-Read TR-F (Lifetime) kit, in accordance with the manufacturer’s instructions. OCR is expressed as µs/µg protein. Data are expressed as the ratio of OCR under hypoxia-mimicking and normoxic conditions.
Statistical analysis
Data are expressed as the mean ± standard deviation (SD) of the indicated number of replicates. Group differences were analysed using Welch’s t-test, and P < 0.05 was regarded as significant.
Data Availability
The datasets generated and/or analysed during the current study are available from the corresponding author on reasonable request.
References
Prasad, S., Gupta, S. C. & Tyagi, A. K. Reactive oxygen species (ROS) and cancer: Role of antioxidantive nutraceuticals. Cancer Lett 387, 95–105 (2017).
Ray, P. D. et al. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal 24, 981–990 (2012).
Guzy, R. D. et al. Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing. Cell Metab 1, 401–408 (2005).
Chandel, N. S. et al. Reactive oxygen species generated at mitochondrial complex III stabilize hypoxia-inducible factor-1a during hypoxia. J Biol Chem 275, 25130–25138 (2000).
Georgieva, E. et al. Mitochondrial dysfunction and redox imbalance as a diagnostic marker of “Free Radical Diseases”. ANTICANCER RES 37, 5375–5381 (2017).
Tochhawng, L., deng, S., Pervaiz, S. & Yap, C. T. Redox regulation of cancer cell migration and invasion. Mitochondrion 13, 246–253 (2013).
Yang, W., Zou, L., Huang, C. & Lei, Y. Redox regulation of cancer metastasis: Molecular signaling and therapeutic opportunities. DRUG DEV RES 75, 331–341 (2014).
Thannickal, V. J. & Fanburg, B. L. Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol 279, L1005–L1028 (2000).
Zepeda, A. B. et al. Cellular and molecular mechanisms in the hypoxic tissue: role of HIF-1 and ROS. Cell Biochem Funct 31, 451–459 (2013).
Hockel, M. & Vaupel, P. Tumor Hypoxia: Definitions and Current Clinical, Biologic, and Molecular Aspects. J Natl Cancer Inst 93, 266–276 (2001).
Majmundar, A. J., Wong, W. J. & Simon, M. C. Hypoxia inducible factors and the response to hypoxic stress. Mol Cell 40, 294–309 (2010).
Semenza, G. L. Hypoxia-inducible factor 1: Regulator of mitochondrial metabolism and mediator of ischemic preconditioning. Biochim Biophys Acta 1813, 1263–1268 (2011).
Papandreou, I., Cairns, R. A., Fontana, L., Lim, A. L. & Denko, N. C. HIF-1 mediates adaptation to hypoxia by actively down regulating mitochondrial oxygen consumption. Cell Metab 3, 187–197 (2006).
Lu, X. & Kang, Y. Hypoxia and hypoxia-inducible factors (HIFs): master regulators of metastasis. Clin Cancer Res 16, 5928–5935 (2010).
Rohwer, N. & Cramer, T. HIFs as central regulators of gastric cancer pathogenesis. Cancer Biol Ther 10, 383–385 (2010).
Kitajima, Y. & Miyazaki, K. The Critical Impact of HIF-1α on Gastric Cancer Biology. Cancers (Basel) 5, 15–26 (2013).
Chandel, N. S. et al. Mitochondrial reactive oxygen species trigger hypoxia-induced transcription. Proc. Natl. Acad. Sci. USA 95, 11715–11720 (1998).
Gao, X. & Schöttker, B. Reduction-oxidation pathways involved in cancer development: a systematic review of literature reviews. Oncotarget 8, 51888–51906 (2017).
Movafagh, S., Crook, S. & Vo, K. Regulation of Hypoxia-Inducible Factor-1a by Reactive Oxygen Species: New Developments in an Old Debate. J. Cell. Biochem 116, 696–703 (2015).
Ishikawa, K. et al. ROS-Generating Mitochondrial DNA Mutations Can Regulate Tumor Cell Metastasis. Science 320, 661–664 (2008).
Ashrafi, G. & Schwarz, T. L. The pathways of mitophagy for quality control and clearance of mitochondria. Cell Death Differ 20, 31–42 (2013).
Kim, I., Rodriguez-Enriquez, S. & Lemasters, J. J. Minireview: Selective Degradeation of Mitochondria by Mitophagy. Arch Biochem Biophys 462, 245–253 (2007).
Boland, M. L., Chourasia, A. H. & Macleod, K. F. Mitochondrial dysfunction in cancer. Front Oncol 3, 1–28 (2013).
Chourasia, A., Boland, M. L. & Macleod, K. F. Mitophagy and cancer. Cancer Metab 3, 4 (2015).
Zhang, H. et al. Mitochondrial Autophagy Is an HIF-1-dependent Adaptive Metabolic response to Hypoxia. J Biol Chem 283, 10892–10903 (2008).
Bellot, G. et al. Hypoxia-Induced Autophagy Is Mediated through Hypoxia-Inducible Factor Induction of BNIP3 and BNIP3L via Their BH3 Domains. Mol Cell Biol 29, 2570–2581 (2009).
Chourasia, A. H. et al. Mitophagy defects arising from BNip3 loss promote mammary tumor progression to metastasis. EMBO reports 26, 1145–1163 (2015).
Liu, L. et al. Mitochondrial outer-membrane protein FUNDC1 mediates hypoxia-induced mitophagy in mammalian cells. Nat Cell Biol, https://doi.org/10.1038/ncb2422 (2012).
Miyamoto, Y. et al. Possible Existence of Lysosome-Like Organella within Mitochondria and Its Role in Mitochondrial Quality Control. PLoS ONE 6, e16054 (2011).
Nakamura, Y. & Arakawa, H. Discovery of Mieap-regulated mitochondrial quality control as a new function of tumor suppressor p53. Cancer Sci, https://doi.org/10.1111/cas.13208 (2017).
Nakamura, Y. et al. BNIP3 and NIX Mediate Mieap-Induced Accumulation of Lysosomal Proteins within Mitochondria. PLoS ONE 7, e30767 (2012).
Kitamura, N. et al. Mieap, a p53-Inducible Protein, Controls Mitochondrial Quality by repairing or Eliminating Unhealty Mitochondria. PLoS ONE 6, e16060 (2011).
Shida, M. et al. Impaired mitophagy activates mtROS/HIF-1α interplay and inceases cancer aggressivenesss in gastric cancer cells under hypoxia. Int J Oncol 48, 1379–1390 (2016).
Kamino, H. et al. Mieap-regulated mitochondrial quality control is frequently inactivated in human colorectal cancer. Oncogenesis 5, e181, https://doi.org/10.1038/oncsis.2015.43 (2016).
Yokozaki, H. Molecular characteristics of eight gastric cancer cell lines established in Japan. Pathol Int 50, 767–777 (2000).
Wansheng, I. et al. Role of p53β in the inhibition of proliferation of gastric cancer cells expressing wild-type or mutated p53. Mol Med Rep 12, 691–695 (2015).
Garziera, M. et al. Identification of novel somatic TP53 mutations in patients with high-grade serous ovarian cancer (HGSOC) using next-generation sequencing (NGS). Int. J. Mol. Sci 19, 1510, https://doi.org/10.3390/ijms19051510 (2018).
Yanagihara, K. et al. Development and biological analysis of peritoneal metastasis mouse models for human scirrhous stomach cancer. Cancer Sci 96, 323–332 (2005).
Miyake, S. et al. HIF-1α is a crucial factor in the development of peritoneal dissemination via natural metastatic routes in scirrhous gastric cancer. Int J Oncol 43, 1431–1440 (2013).
Acknowledgements
We would like to thank Mr. S Nakahara for his valuable contribution to the electron microscopic analysis. We also thank Anne M. O’Rourke, PhD, from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript. This study was financially supported by JSPS KAKENHI Grants-in-Aid for Scientific Research (Research Project no. 18K08650).
Author information
Authors and Affiliations
Contributions
Y.K. and K.O. conceived and designed the experiments. K.O., N.E., H.K. and K.I. performed the experiments. Y.K., K.O. and T.T. analysed the data. S.A. and K.Y. contributed reagents, materials or analytical tools. K.O., Y.K. and H.N. wrote the paper. All authors discussed the results and commented on the manuscript.
Corresponding author
Ethics declarations
Competing Interests
The authors declare no competing interests.
Additional information
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Okuyama, K., Kitajima, Y., Egawa, N. et al. Mieap-induced accumulation of lysosomes within mitochondria (MALM) regulates gastric cancer cell invasion under hypoxia by suppressing reactive oxygen species accumulation. Sci Rep 9, 2822 (2019). https://doi.org/10.1038/s41598-019-39563-x
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-019-39563-x
- Springer Nature Limited
This article is cited by
-
Analysis of human neuronal cells carrying ASTN2 deletion associated with psychiatric disorders
Translational Psychiatry (2024)
-
Hypoxia-reprogramed megamitochondrion contacts and engulfs lysosome to mediate mitochondrial self-digestion
Nature Communications (2023)
-
The emerging mechanisms and functions of microautophagy
Nature Reviews Molecular Cell Biology (2023)
-
Energy metabolism: a new target for gastric cancer treatment
Clinical and Translational Oncology (2023)