Abstract
Maternal lipolytic metabolic disorders result in a lipotoxic microenvironment in the ovarian follicular fluid (FF) which deteriorates oocyte quality. Although cellular stress response mechanisms are well defined in somatic cells, they remain largely unexplored in oocytes, which have distinct organelle structure and nuclear transcription patterns. Here we used shotgun proteomic analyses to study cellular responses of bovine oocytes and cumulus cells (CCs) after in vitro maturation under lipotoxic conditions; in the presence of pathophysiological palmitic acid (PA) concentration as a model. Differentially regulated proteins (DRPs) were mainly localized in the endoplasmic reticulum, mitochondria and nuclei of CCs and oocytes, however the DRPs and their direction of change were cell-type specific. Proteomic changes in PA-exposed CCs were predominantly pro-apoptotic unfolded protein responses (UPRs), mitochondrial and metabolic dysfunctions, and apoptotic pathways. This was also functionally confirmed. Interestingly, although the oocytes were enclosed by CCs during PA exposure, elevated cellular stress levels were also evident. However, pro-survival UPRs, redox regulatory and compensatory metabolic mechanisms were prominent despite evidence of mitochondrial dysfunction, oxidative stress, and reduced subsequent embryo development. The data provides a unique insight that enriches the understanding of the cellular stress responses in metabolically-compromised oocytes and forms a fundamental base to identify new targets for fertility treatments as discussed within.
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Introduction
Maternal metabolic disorders such as obesity and type-II diabetes are increasing in prevalence and have been strongly linked with reduced fertility1 and IVF success rates2,3. Trials using metabolically-healthy donor oocytes could improve the fertility of those patients4,5. Thus, direct effects on oocyte quality is considered an important link between maternal metabolic diseases and reduced fertility. Understanding the underlying mechanisms that occur at the oocyte level is important to increase treatment efficiency.
The ovarian follicular fluid (FF) composition is significantly altered by metabolic factors2,6. Upregulated lipolysis is common in metabolic disorders e.g. obesity and type-II diabetes due to reduced insulin sensitivity. Elevated non-esterified free fatty acid (FFA) concentrations in blood, predominantly; palmitic (PA), stearic, linoleic and oleic acids are reflected in the FF2,7. Particularly, high levels of PA in FF was linked with negative pregnancy outcome following ICSI8. PA-induced lipotoxicity causes various pathological conditions e.g. cardiovascular and neurological diseases9. We have previously shown, using mouse and bovine models, that in vitro exposure to pathophysiological concentrations of FFAs compromise oocyte quality and significantly reduce oocyte developmental competence10,11, leading to lower proportions of transferable embryos, higher apoptotic cell indices12, and significant alterations in embryo epigenetic and transcriptomic profiles13.
Cumulus cells have been shown to accumulate cytoplasmic lipid droplets from their microenvironment14. Intracellular lipid accumulation in somatic cells causes abnormal cellular metabolism, lipid peroxidation, leading to oxidative stress and protein misfolding15. Accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress that elicit specific “unfolded protein response” (UPRer)16. This is evident in FFA-treated bovine COCs in vitro17 particularly in CCs12 and was associated with reduced embryo development rates. Similar responses occur in the mitochondria (UPRmt)18. These UPRs stimulate nuclear expression of chaperons to maintain cellular survival or induce programmed cell death if stress level is intolerable19. Although our understanding of these mechanisms is expanding in somatic cells, it remains largely unexplored in oocytes, which have different organelle composition and nuclear transcription compared to somatic cells20. Studying these regulatory mechanisms in details in oocytes involves complex networks and can be challenging considering the scarceness of samples and ethical limitations in humans. Bovine oocytes are available in large numbers from slaughter house material and share many functional similarities with human oocytes e.g. the duration of folliculogenesis, oocyte diameter, lipid content, timing of maturation, early cleavage, and embryo genome activation21, and thus is proposed to be a good model for studying molecular responses to metabolic stress.
We have previously observed differential expression of few genes related to lipid and carbohydrate metabolism between CCs and oocytes derived from FFA-treated bovine COCs22. However, considering that most cellular stress responses involve changes at the protein level23, transcriptomic analysis may not be comprehensive. Recent advancement of proteomic analysis has increased the sensitivity and accuracy of identification and quantification of proteins in small sample size. Therefore, the aim of this study was to analyze and compare cellular responses to PA-induced lipotoxicity during in vitro maturation of bovine COCs using shotgun proteomic analysis of CCs and of the enclosed-oocytes. This was complemented by other functional tests of mitochondrial activity, oxidative stress levels, cellular apoptosis and a follow up of subsequent early embryo development.
Results
Effect of PA-exposure during IVM on oocyte developmental competence and embryo quality
Good quality bovine COCs were isolated and selected from ovaries of slaughtered cows and matured in vitro in the presence of a pathophysiological concentration of PA (150 µM) or solvent (ethanol, 0.01%). PA significantly (P < 0.05) reduced total cleavage rates and proportions of good-quality embryos (4-cell-stage or beyond) at 48 h post-insemination (p.i.), while embryo fragmentation was increased compared to the solvent controls (P < 0.05). At day 8, blastocyst rates were significantly lower in the PA group compared to the solvent control (P < 0.05) despite fertilization and culture in a FA-free condition (Table 1). We have previously shown that ethanol (0.01%) does not affect oocyte quality or embryo development if compared to solvent-free control12. PA-derived blastocysts were lower in quality as they exhibited higher percentage of apoptotic cells (CASP3 positive), mainly in the inner cell mass (ICM), compared to solvent control. The average counts of total, ICM, and trophectoderm (TE) cells were not affected by the treatment as determined by differential immunostaining with CDX2 and Hoechst.
Effect of PA on apoptosis in COCs
Apoptosis was higher (P < 0.05) in CCs in the PA-exposed COCs compared to those in solvent control. 11/34 (32.3%) of PA-COCs had ≥25–50% of their CCs positively stained to CASP3, and 17/34 (50%) of the COCs had 10–25% of the cells apoptotic. Whereas, the majority of COCs in solvent control (34/35, 97.1%) showed very low CASP3 staining (0–5%). No CASP3 positive staining could be detected in the oocytes in both treatment groups (Fig. 1).
Effect of PA on mitochondrial activity and oxidative stress in COCs
CellRoxTM deep red staining intensity (as an estimate for intracellular reactive oxygen species, ROS) was significantly higher in PA-treated CCs (29.7 ± 3.87 vs. 17.5 ± 0.74, P < 0.05) compared with the solvent controls. This staining was done in combination with JC1 to determine mitochondrial activity. J-aggregates: monomers (590: 525 nm) ratio (as an estimate of inner mitochondrial membrane potential, MMP) was also significantly higher in PA-treated CCs (108.5 ± 4.10% vs. 74.7 ± 4.71% in solvent control). However, we noticed that JC-1 and CellRox staining intensity in PA-treated CCs were markedly heterogeneous within the same COC while more homogenous in control COCs (Fig. 2A). Interestingly, some cells exhibiting high oxidative stress level (CellRox staining, red) had the lowest J-aggregate fluorescence intensity (yellow) suggesting loss of MMP, i.e. uncoupling (see cells indicated with arrows, Fig. 2B). Other CCs exhibiting high MMP had high CellRox staining (arrow heads, Fig. 2A).
In oocytes, MMP was significantly higher in the PA group compared to controls; where the average J-aggregates: monomer ratio was 144 ± 4.1 vs. 123 ± 5.1 (P < 0.05). CellRox intensity was also higher in PA-oocytes (43.3 ± 2.36 vs. 28.4 ± 2.43, P < 0.05) indicating higher levels of oxidative stress (Fig. 2B). An obvious co-localization was noticed between CellRox and JC-1 staining in the oocytes in both treatment groups (see merged images).
TMT proteomic analysis: Differentially regulated proteins in response to PA-treatment
A total of 1703 and 1185 proteins were identified in CCs and oocytes, respectively, 679 of which were common. P values and fold changes of these proteins are depicted in the volcano plots in Fig. 3. Exposure to PA resulted in a significant change in the relative abundance of 86 and 54 proteins (DRPs) in CCs and oocytes, respectively, compared to the solvent group. Only 4 DRPs were common in CCs and oocytes, which shows distinct cell type-dependent responses. Hierarchical clustering of the relative abundance of DRPs using MeV software showed close clustering of the three PA samples apart from the three control samples within the oocyte and CC databases.
Cellular localization of differentially regulated proteins
The majority of the DRPs of CCs and oocytes were localized in ER, mitochondria and nuclei. Most of the DRPs in CCs were down-regulated in the PA group compared with solvent, while the majority of the DRPs in oocytes were upregulated. (Table 2).
Biological functions and Canonical pathway analysis
Core analysis in IPA (Qiagen Bioinformatics) showed that the PA-induced DRPs of CCs are involved in several canonical pathways related to cellular stress and metabolism. The top canonical pathways in CCs and the proteins involved in each pathway are listed in Fig. 4a. Interestingly, UPR, Sirtuin Signalling Pathway and Mitochondrial dysfunction pathways were the most affected. Other related pathways were also significantly induced by PA in CCs and include protein ubiquitination, EIF2 and mTOR signaling which are in line with the UPRs, as well as the influence on proteins involved in glycolysis, FA β-oxidation, and oxidative phosphorylation which altogether indicates a dysfunction in cellular metabolism.
In oocytes, UPR and mitochondrial dysfunction were also among the top pathways affected by PA treatment. However, interestingly, other specific pathways were prominent (Fig. 4b). The top significant pathway was the p70S6K signaling. Other affected pathways related to oxidative stress regulation were also significant e.g. glutathione redox regulation and NRF2-mediated oxidative stress response. To avoid repetition, these pathways are shown in Fig. 4 and described in the discussion. Lists of up and down regulated proteins in CCs and oocytes, and their individual biological functions, fold changes and P values are shown in details in Supplementary Tables 1a–d in Appendix 2.
A comparison analysis of PA-induced DRPs in CCs vs. oocytes was also done in IPA (Qiagen Bioinformatics) (Fig. 5). Although the detected individual DRPs are different, many altered pathways were common. Interestingly, UPRs and mitochondrial dysfunction, as well as several metabolic pathways were among those common pathways. However, as shown in Fig. 4 and described in the discussion, the direction of change was opposite, particularly in UPR and ER stress pathways. Some pathways were exclusively or highly altered in CCs compared with the oocytes such as the Sirtuin signaling, pentose phosphate pathway (PPP), oxidative phosphorylation, and fatty acid β-oxidation. Whereas, other pathways were exclusively or highly altered in the oocytes such as p70S6K and 14-3-3-mediated signaling, and NRF-2 mediated oxidative stress response.
Discussion
In the present study, we used TMT-labeled shotgun proteomics to gain a comprehensive understanding of the molecular stress response mechanisms in oocytes and CCs to PA-induced lipotoxicity. This experimental setup was used as a model for the lipotoxic follicular microenvironment in patients with lipolytic disorders where predominantly PA is elevated2,8. In vitro models provide limited representation of the follicular fluid biochemical alterations that occur in vivo, where interactions between different factors such as FFAs and other nutrients are evident and described2,24,25,26,27,28. However, strategically designed in vitro models enable focus on specific factors, pathways or mechanisms. The present study is a first trial to understand the stress response mechanisms to a lipotoxic insult in oocytes using a novel proteomic insight. We only focused on PA-induced lipotoxic effects to reduce the number of fixed factors, increase statistical power and allow accurate interpretation of the complex proteomic data in combination with other functional assessments. Interactions with other FFAs and hyperglycemic conditions during IVM have been described in our previous studies11,12,28.
Bearing in mind the complexity of the proteomic data, we focus in this discussion on top pathways and networks of biological relevance, and make speculations based on the functional annotations of the involved proteins and on the associated functional phenotypes. Detailed lists of the DRPs and their specific biological functions are supplemented for deeper insight.
First focusing on the responses in CCs, PA induced several alterations in cellular metabolism-related proteins, the majority of which were mitochondrial. Interestingly, three complementary subunits of the mitochondrial ATP synthase enzyme were simultaneously downregulated; ↓ATP5H is subunit d of the mitochondrial inner membrane-spanning component of ATP synthase complex; and ↓ATP5A1 and ↓ATP5B, which are subunits α1 and β of the soluble catalytic core of the enzyme complex. Mitochondrial Cytochrome c oxidase (Complex IV) subunit 5A (COX5A) was also downregulated. The reduced availability of complex IV and V subunits may have negative effects on the efficiency of the electron transport chain and ATP synthesis during oxidative phosphorylation in CCs. In addition, both lactate dehydrogenase A and B (LDHA and LDHB) were upregulated together with ↑PGK1 and ↑PKM suggesting higher rates of glycolysis and lactate production. We have previously shown that bovine COCs matured in high concentrations of palmitic, stearic and oleic acids (High NEFAs) had significantly higher LDHA mRNA expression in CCs22, and that bovine COCs matured under diabetes-like conditions (high glucose and NEFAs) produced more lactate during culture29. This is a mechanism known as the Warburg effect, which is common in cancer cells and has been described in non-cancerous conditions in association with tissue damage such as in meningitis, encephalitis, and acute pancreatitis30. Metabolism of glucose through the pentose phosphate pathway (PPP) also appears to be upregulated here in PA-treated CCs as suggested by ↑PGD, a member of the oxidative branch of PPP that generates NADPH, which in turn reduces glutathione to prevent oxidative stress. The NADPH-dependent enzyme, AKR1B1, that catalyzes reduction of aldehydes and ketones was also upregulated. ↑AKR1B1 has been linked to the pathogenesis of secondary diabetic complications such as neuropathy and nephropathy31. In the non-oxidative phase of PPP, ↑transaldolase 1 was observed, which is needed to generate pentoses for nucleotide synthesis.
In addition to these modifications in carbohydrate metabolism, FA β-oxidation (FAO) is also affected as suggested by ↓SCP2, a mitochondrial non-specific lipid transfer protein, and ↓HADHA, an inner membrane (IMM) mitochondrial trifunctional protein that is physically associated with complex I32. In addition, ↑FAF2, a lipase inhibitor, may inhibit triacylglycerol hydrolysis in PA-treated CCs. This may explain the increased lipid accumulation in NEFA-treated COCs24. Perhaps this reduced metabolic activity in the affected CCs is a cellular response to control oxidative stress, which is evident here as shown by the increased CellRox staining intensity in the PA group. Oxidative stress has been shown to reduce the relative abundance of complex I proteins in insulin-resistant skeletal cells that are involved in FAO33.
Besides the alterations in metabolism-related mitochondrial proteins, subunits of the “mitochondrial contact site and cristae organizing system” (MICOS): CHCHD3 and IMMT, were significantly reduced. This system is crucial for the formation and maintenance of cristae structure, and is involved in the formation of contact sites to the outer mitochondrial membrane (OMM) for fusion processes34. This may lead to deteriorated mitochondrial ultrastructure as reported in COCs of high fat diet-induced obese mice35.
As an expected consequence of oxidative stress, several ER and ribosomal proteins were dysregulated in CCs that are related to regulation of translation, protein transport, translocation and post-translational protein modification, as well as ER-to-golgi vesicle-mediated transport. This confirms the involvement of ER stress in the pathogenesis of lipotoxicity in CCs as previously suggested by Sutton-McDowall, et al.17. However, only few of these changes were pro-survival, while many pro-apoptotic changes were detected. Pro-survival changes include ↑FAF2 needed for ubiquitin-dependent ER-associated protein degradation “ERAD”; ↑RPS3 that is involved in HSP70 and HSP90 mediated regulation of translation; and ↓ERO1A36. EIF2α signaling was also upregulated, which is a rapid response to arrest protein translation37. In contrast, crucial pro-survival chaperons and proteins related to the UPRer were downregulated in PA-exposed CCs, namely CALR, P4HB, HSP90B1, PDIA6, HSPA9, and CANX. This strongly suggests that ER-stress-induced cell death mechanisms are actively involved. High levels of ER stress can induce a process known as “regulated IRE1α-dependent decay” which degrades mRNA encoding for crucial proteins such as ER chaperons38,39. Programmed cell death in PA-treated CCs could also be facilitated by ↓VDAC2 (mitochondrial), ↓LRPAP1 (ER) and, ↓LMNA (nuclear), which are negative regulators of apoptosis. Previous reports have shown that PA induces apoptosis in granulosa cells40. Similarly, high percentage of CCs in the PA-treated COCs were apoptotic (positive to CASP3), but not all, which illustrates that different levels of ER stress are evident in PA-CCs. A heterogeneous pattern of mitochondrial activity and oxidative stress in different CCs within the same COC were also observed using JC1 and CellRox staining. These data suggest that preventative treatments aiming to reduce oxidative stress levels, inhibit ER stress signaling or enhance mitochondrial functions in CCs may protect oocyte developmental competence.
Now focusing on the oocytes, although they were enclosed (and protected41) by CCs during exposure to PA during IVM, significant alteration in the proteomic profile, oxidative stress levels and mitochondrial activity under PA-conditions in vitro clearly shows that the oocytes are also sensitive and responsive to the lipotoxic maturation conditions. The majority of PA-oocytes were cleaved after fertilization suggesting that the lipotoxic effect was less sever on oocytes compared to CCs. Importantly, although top canonical dysregulated pathways include UPR, mitochondrial dysfunction, lipid metabolism, and oxidative stress, many of these changes were predominantly pro-survival and adaptive mechanisms. The majority of the DRPs belong to ER and mitochondria demonstrating that these organelles play a key regulatory role in stress responses in oocytes.
In contrast to the changes in metabolic proteins in CCs, the relative abundance of the mitochondrial HADHB (complex I32), UQCRB (complex III) and CYCS were increased in oocytes suggesting an increased electron transport activity. This is matching with the increased MMP (JC1-aggregates). HADHB catalyzes three out of the four steps in FAO32. This increased bioenergetic activity may be a compensation for the reduced energy production and altered metabolism in CCs, as suggested by the proteomic changes and the reduced viability. However, ROS production was also increased in the PA-oocytes as shown by the increased CellRox staining. In addition, PA increased the relative abundance of few mitochondrial proteins with antioxidative functions, particularly, Peroxiredoxin 3 (PRDX3) which is a mitochondrion-specific H2O2-scavenging enzyme42. We noticed obvious co-localization of CellRox deep red staining in oocytes with mitochondria (JC1) suggesting that mitochondria are particularly the source of- and most affected by- the oxidative stress. This may also explain why the mitochondrial specific PRDX3 was specifically upregulated. PRDX3 is also required for mitochondrion organization and regulation of MMP42. Interestingly, PRDX3 is not only regulated by cellular redox status but also by acetylation. This can be linked with the reduced abundance of HINT2 in PA-treated oocytes, since HINT2 deletion provoked mitochondrial deformities and changed the pattern of acetylation in Hela cells43. PA-exposure during IVM has been recently reported to increase mitochondrial protein hyperacetylation patterns in porcine oocytes44.
In addition to the evident increase in metabolic activity in the oocyte, pathways related to amino acid metabolism were also significantly affected; ↑QDPR, a cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases, suggests high rates of amino acid degradation. Amino acid degradation is an immediate cellular response to oxidative stress45. Inheritance of sensitized mitochondria with increased metabolic rate and high amino acid turnover from a metabolically-compromised oocyte to the embryo may contribute to the pathogenesis of reduced subsequent embryo development, since the most viable preimplantation embryos were shown to have the lowest metabolic rates, lowest glycolytic rates and/or lowest amino acid turnover (which is known as the “quiet embryo metabolism hypothesis”46).
Importantly, MAPK2K2 appears to play a key role in mediating PA-induced cellular stress in oocytes. Several altered canonical pathways involve MAPK2K2, namely, p70S6K Signaling, EIF2 Signaling, PI3K/AKT Signaling and NRF2-mediated Oxidative Stress Response. Both p70S6K and EIF2 signaling (regulated by mTOR) regulate cell survival, protein synthesis, autophagy, and transcription47. Therefore, these cellular modifications appear to be employed in oocytes to support oocyte viability and increase resistance to lipotoxicity. p70S6K signaling is the top canonical pathway affected in oocytes, which beside interacting with eIF2 pathways for regulation of translation and RNA processing, promotes cell survival through 14-3-3-mediated signaling48. This can be facilitated by the upregulated 14-3-3 protein gamma (YWHAG) noted here. NRF2-mediated Oxidative Stress Response has been recently shown to mediate bovine embryo survival under oxidative-stress conditions49. In addition to MAPK2K2, GSR and STIP1, members of NRF2-mediated response, were upregulated in the PA-treated oocytes. Mouse models with enhanced Nrf2 activation were shown to have increased transcription of Gsr that is involved in GSH biosynthesis and regeneration50. The increase in QDPR may also contribute to the oocyte’s defense mechanism against oxidative stress since it was shown to induce SOD1 and GPX3 expression kidney cells51.
This network of regulatory molecules is further enriched by the fact that both HSPA8 and its co-chaperon, STIP152 are upregulated in PA-oocytes. Biological functions of HSPA8 (see Supplementary Table 1c) involve chaperone cofactor-dependent protein refolding, autophagy, regulation of mRNA stability, which may support oocyte survival. Other UPR proteins also increased in the PA-oocytes, such as those involved in ER stress pathway (↑P4HB, but ↓HSPA5), chaperon mediated protein folding (↑CCT8, ↑FKBP4), protein refolding and regulation of protein stability (↑HSPA8), ubiquitin dependent protein catabolic process (↑COPS3, ↑BCAP31, ↑ELOB), as well as lysosomal chaperon-mediated autophagy (↑HSPA8, ↑CTTN). Negative regulators of apoptosis were also upregulated in the affected oocytes, namely AKR1B1, MYDGF, P4HB, CTTN. Looking further into the data, we found that mitochondrial UPR markers HSPD1 (FC = 1.22, P = 0.065) and HSPE1 (FC = 1.62, P = 0.09) both tended to be upregulated which may suggest that mitochondrial UPR is also involved18.
Finally, the increased abundance of Cytochrome C (CYCS) in the PA-treated oocytes is alarming. Besides its role in energy production, CYCS regulates induction of apoptosis. However, here this should not be necessarily interpreted as an evidence of apoptosis in PA-treated oocytes, especially considering that the majority of these oocytes were developmentally competent. CYCS induce apoptosis when released into the cytosol due to outer mitochondrial membrane (OMM) permeabilization caused by high levels of oxidative and mitochondrial stress53. PA-treated oocytes exhibit ↑SLC24A5, a negative regulator of OMM permeabilization which may prevent apoptosis and support other pro-survival mechanisms in the oocyte. Nevertheless, it is important to consider that the ability of an oocyte to maintain survival under metabolic stress conditions may be reduced later during early embryo development since fertilization may exaggerate intracellular ROS levels54. High oxidative stress during early cleavage stage have been shown to increase embryo fragmentation, which is also noticed here in embryos derived from PA-treated oocytes. Treatments aiming to reduce cellular stress levels at this stage can be thus useful to increase survival during early embryo development.
Collectively, these data show evidence that cumulus-enclosed oocytes are sensitive to lipotoxic changes in their microenvironment. These changes were detected by shotgun proteomics and provide a unique, very informative insight into the oocyte’s response mechanisms to metabolic stress compared with the surrounding CCs. These changes are detectable following a short-term exposure during IVM, which mimics a post-LH phase. The magnitude of these adaptive mechanisms may differ under in vivo conditions where oocytes undergo longer exposure to metabolic stress during follicular growth. Extrapolation of these data to the in vivo settings should therefore be done with caution. We have previously described longer-term exposure effects of FFAs during in vitro murine follicular growth and maturation for 13 days which had similar detrimental impact on oocyte developmental competence compared to short term exposure10.
In conclusion, while DRPs in CCs suggest metabolic failure and pro-apoptotic signaling, proved by mitochondrial dysfunction and increased apoptosis, the enclosed oocytes undergo detectable proteomic compensatory metabolic adaptations and responses to oxidative stress that were predominantly pro-survival. Clearly, UPRer and mitochondrial proteins play an important regulatory role in these adaptive responses in oocytes. In addition, several other prominent proteins appear to contribute, such as PRDX3, HADHB, MAPK2K2, STIP1-HSPA8, as well as the NRF2-mediated oxidative stress response, p70S6K and 14-3-3 mediated signaling. The majority of these responses have not been previously studied in oocytes. Nevertheless, high levels of CYCS and oxidative stress makes these oocytes vulnerable during subsequent development if not handled under supportive conditions. These data enriches the current understanding of the mechanisms by which the oocyte can respond to metabolic stress and forms a fundamental base for subsequent studies to focus on the specific role of each of these pathways in metabolically-compromised oocytes. The study also highlights avenues through which the developmental capacity of metabolically-compromised oocytes may be improved, protected or rescued.
Materials and Methods
Collection of cumulus oocyte complexes
Bovine ovaries from a local abattoir were transferred to our laboratory within 2 h of slaughter in warm saline. Ovaries were washed and visible antral follicles (2–6 mm in diameter) were aspirated. Follicular fluid was allowed to set for 5 min and the cellular precipitate was transferred to a searching dish containing Hepes-buffered Wash-TALP media as described by Van Hoeck, et al.11. Good quality COCs (with dark, homogenous ooplasm and more than 5 layers of compact cumulus cells) were selected and used in the experiments.
In vitro maturation and treatments
Stock solutions of PA (150 mM) were prepared in ethanol. Maturation media was composed as described earlier13 based on TCM-199 media supplemented with 0.75%w/v FA-free BSA as a carrier for PA, and murine epidermal growth factor (mEGF, 20 ng/ml) to stimulate oocyte maturation. Selected COCs were washed and cultured in groups of 50 ± 5 COCs in 4-well plates containing maturation media (500 µL/well) supplemented with PA (150 µM) or solvent (ethanol, 0.01%) for 24 h in 5% CO2 in humidified air at 38.5 °C. This PA concentration is pathophysiologically relevant considering the elevated PA concentration in FF in obese women55 and in cows during negative energy balance56. COCs were then either fertilized and further cultured to examine subsequent developmental competence, or separated into CCs and oocytes and snap-frozen for proteomic analysis, or used for other functional parameters to assess oocyte quality and cumulus cell viability.
Immunohistochemical determination of apoptosis in COCs
At 22 h of IVM, PA and solvent COCs (n = 69 from 3 replicates) were fixed in paraformaldehyde 4% for 1 h then stored in PBS containing PVP (1 mg/mL) at 4 °C. Cumulus cell apoptosis was determined by immunostaining with rabbit-anti-cleaved Caspase-3 (CASP3, Asp 175, Cell Signaling Technology, 1:250) as previously described12. Images were obtained using a fluorescence Olympus microscope IX71. The percentage of positively stained cells were determined using ImageJ as described by Jensen57 within each COC. COCs were categorized according to the percentage of apoptotic cells (as 0–5%, 5–10%, 10–25%, 25–50%, >50%).
Assessment of mitochondrial activity and intracellular reactive oxygen species in COCs
A combined fluorescence staining technique using 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolyl-carbocyanine iodide (JC-1, Invitrogen) and CellROX™ Deep Red Reagent (ThermoFisher) were used (as validated and described earlier by De Biasi, et al.58 and Komatsu, et al.59) to estimate mitochondrial inner membrane potential (MMP) and oxidative stress, respectively, in the oocytes and surrounding CCs. In 3 replicates, Control and PA COCs were transferred at 22 h of IVM to a Petri dish in a droplet of Wash-TALP media at 38 °C on a warm-stage. COCs (n = 42) were denuded using EZ-tip (125 µm) attached to an EZ-Grip micropipettor (Origio). Other COCs (n = 46) were used for imaging of CCs. Oocytes and COCs were incubated in maturation media containing JC1 (5 µg/mL) and CellRox deep red (2.5 mM) (from 1000X stock solutions in DMSO) for 30 min at 5% CO2 and 38.5 °C. They were then washed and kept in wash-TALP droplets under oil in a 35 mm dish with glass bottom and immediately examined under a Leica SP8 confocal microscope equipped with white laser source (Leica WLL) lasers at excitation/emission 488/525 nm (to detect JC-1 monomers; green), 561/590 nm (JC1-aggregates; yellow) and 644/665 nm (to visualize CellRox staining). The microscope was enclosed in a humid chamber adjusted at 37 °C. One optical section was examined for each oocyte at the pericortical area of the oocyte or at the level of CCs. The grey scale intensity in each channel was measured using Leica Application Suite X (LAS X) software. Florescence intensities in oocytes were determined only in the area of the ooplasm, i.e. excluding the zona pellucida which may contain remnants of mitochondria from the CC extrusions. MMP was calculated as the ratio of the grey scale intensity at 590 nm:525 nm. Oxidative stress was estimated based on the grey scale intensity at 665 nm.
In vitro fertilization and embryo culture
A total of 603 COCs in four replicates were used to study developmental competence. For that, at 24 h of IVM, COCs were fertilized (in Fert-TALP medium, groups of 100 ± 10 COCs, 22 h) and cultured (in mSOF, groups of 25 ± 5 embryos, 7 days) as previously described12. Sample size calculation for the number of COCs used to determine developmental competence was calculated based on a power of 80% to detect a 10% difference in blastocyst rates between the PA and solvent groups at P = 0.05 (PS: Power and Sample Size Calculation version 3.1.6, Vanderbilt Biostatistics). At 48 h pi, proportions of cleaved embryos, good quality embryos (≥4-cells) and those with >15% fragmentation of cellular mass were recorded. Blastocyst rates (8 days p.i.) were recorded and expressed as a proportion of total number of oocytes. Morphological classification was done under an inverted Olympus CKX41 microscope (Olympus, Aartselaar, Belgium). Blastocysts were fixed on Day 8 in 4% paraformaldehyde (30 min) and stored in PBS containing PVP (1 mg/ml) for quality assessment using differential immunostaining staining with CASP3 antibody (apoptosis), anti-CDX2 antibody (for trophectoderm cell count), and Hoechst (for total cell count) as previously described12.
Proteomic analysis of CCs and oocytes
A TMT shotgun-MS proteomic analyses was performed on protein extracts of pools of 120–200 oocytes or their corresponding CCs (3 replicates). Sample preparation, protein extraction, digestion and TMT labelling, and Nano reversed phase liquid chromatography and mass spectrometry were done as described in supplementary file (Appendix 1). Spectra were analyzed in Proteome discoverer (2.1) software against Bos Taurus database using only medium and high confident peptides with a global FDR <5% based on a target-decoy approach60 and normalization was done by the CONSTANd algorithm61 (see Appendix 1 for more details). Differentially regulated proteins (DRPs) were determined based on a 5% FDR, adjusted P value <0.05, and more than 10% fold change in PA compared to control groups. Heat maps and hierarchical clustering of the proteomics data were performed using MultiExperimentViewer (MeV) version 4.9.0 (Bio-Soft Net). GO-Cellular component analysis was performed in Panther classification system (Panther13.1) and by manual extraction of subcellular location data from the UniProt database if available (uniprot.org). Canonical pathways of the DRPs and comparison analysis between PA effects in CCs and oocytes were performed using Core Analyses in Ingenuity® Pathway Analysis (IPA, QIAGEN bioinformatics).
Statistical analysis
Cleavage and blastocyst rates were analysed using a binary logistic regression models in IBM SPSS Statistics 23 (for Windows, Chicago, IL, USA). Numerical data e.g. from JC1 staining and CellRox quantification were analysed using linear mixed models. In all analyses, replicates, treatments and their interactions were taken into account. The interactions had no significant effects and were omitted from the models. A Bonferroni post-hoc test was performed to correct for multiple comparisons. All data were from at least three independent replicates. Differences of P values ≤ 0.05 were considered significant and those >0.05 and ≤0.1 were reported as tendencies.
Data Availability
The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request. Proteomics data are available via ProteomeXchange with identifier PXD012141.
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Acknowledgements
WFA Marei is supported by Research Foundation - Flanders (FWO) postdoctoral fellowship grant number 12I1417N.
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W.F.A.M. designed and performed the experiments, performed all the outcome parameter assessments except the proteomic analysis, analyzed and interpreted the data and did functional annotation analysis, and drafted the manuscript. G.V.R. and G.B. performed the proteomic analysis and the associated data normalization and analysis. P.E.L.B. critically revised the manuscript and contributed to the writing, J.L.M.R. is the project supervisor and contributed to the main idea, experimental design and critically revised and edited the manuscript.
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Marei, W.F.A., Van Raemdonck, G., Baggerman, G. et al. Proteomic changes in oocytes after in vitro maturation in lipotoxic conditions are different from those in cumulus cells. Sci Rep 9, 3673 (2019). https://doi.org/10.1038/s41598-019-40122-7
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DOI: https://doi.org/10.1038/s41598-019-40122-7
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