Abstract
Nipah virus (NiV), an emerging zoonotic pathogen in Southeast Asia, is transmitted from Pteropus species of fruit bats to a wide range of species, including humans, pigs, horses, dogs, and cats. NiV has killed millions of animals and caused highly fatal human outbreaks since no vaccine is commercially available. This study characterized the immunogenicity and safety of poxvirus-based Nipah vaccines that can be used in humans and species responsible for NiV transmission. Mice were vaccinated with modified vaccinia Ankara (MVA) and raccoon pox (RCN) viral vectors expressing the NiV fusion (F) and glycoprotein (G) proteins subcutaneously (SC) and intranasally (IN). Importantly, both vaccines did not induce significant weight loss or clinical signs of disease while generating high circulating neutralizing antibodies and lung-specific IgG and IgA responses. The MVA vaccine saw high phenotypic expression of effector and tissue resident memory CD8ɑ+ T cells in lungs and splenocytes along with the expression of central memory CD8ɑ+ T cells in lungs. The RCN vaccine generated effector memory (SC) and tissue resident (IN) CD8ɑ+ T cells in splenocytes and tissue resident (IN) CD8ɑ+ T cells in lung cells. These findings support MVA-FG and RCN-FG viral vectors as promising vaccine candidates to protect humans, domestic animals, and wildlife from fatal disease outcomes and to reduce the global threat of NiV.
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Introduction
Nipah virus (NiV), a paramyxovirus from the genus Henipavirus, is a zoonotic pathogen that poses a severe global threat to human and animal health1. NiV is an enveloped single-stranded RNA virus encoding six structural and three nonstructural proteins, with fusion (F) and glycoprotein (G) membrane-anchored proteins that are the key antigens for NiV vaccine development2. Its natural hosts and wildlife reservoirs are Pteropus fruit bats, including P. lylei, P. medius, P. vampyrus, and P. hypomelanus species3,4,5,6. The first NiV infection was reported in Malaysia in 1998, followed by outbreaks in multiple countries throughout South and Southeast Asia, including recent cases reported in India, from May to June 20187,8. Infected bats can transmit the virus to various species such as humans, pigs, horses, cats, dogs, and other domestic animals through contact with bat body fluids or the consumption of partially eaten fruits from infected bats9. In humans, NiV infection has been associated with the consumption of horse meat, close contact with pigs and horses, proximity to other infected humans, and ingestion of bat-contaminated date palm sap10,11,12.
The NiV bat reservoir host experiences no symptoms. In contrast, human infections range from asymptomatic to fatal encephalitis and severe respiratory disease, with a fatality rate between 40–75% 9. In the absence of an available NiV vaccine, supportive care, and the use of the antiviral ribavirin in patients with acute encephalitis have reduced mortality by up to 36%13. In swine, NiV infection can cause neurological and respiratory symptoms such as a hacking cough and muscle spasms, reaching a mortality rate as high as 40%14.
Because of its broad host range, various modes of transmission, and high fatality rate, NiV has been categorized as a priority pathogen and prompting vaccine development. In this regard, various NiV vaccine strategies have been developed, including subunit vaccines, recombinant virus vectors, virus-like particles (VLP), and mRNA platforms15. However, these approaches mainly focus on protecting human health, making them fall short of addressing a One Health-based vaccine approach, a collaborative effort to protect all facets of human, animal, and environmental health. Moreover, few NiV vaccine candidates have tested mucosal delivery methods, significantly reducing their practical application in wild and domestic animal populations, ideal for suppressing the virus before wide-scale outbreaks occur.
Poxviruses are double-stranded DNA viruses that belong to the Poxviridae family. Several characteristics make them an excellent antigen delivery platform for recombinant vaccine candidates. For instance, they can accept large foreign DNA fragments, thus allowing them to co-express several foreign antigens16. Also, poxviruses replicate in the cytoplasm of host cells, which eliminates the risk of potential integration into the host genome. Additionally, they can infect via mucosal and dermal routes and induce specific cell-mediated and humoral immune responses against transgenes17.
Modified vaccinia virus Ankara (MVA) is a highly safe poxvirus used in humans during the smallpox eradication program18. It has been evaluated as a delivery platform in vaccine candidates, expressing bacterial, parasites, or viral antigens and inducing protective immune responses19,20,21. Another safe and effective poxvirus vector is raccoon poxvirus (RCN). RCN was first isolated from the upper respiratory tract of apparently healthy raccoons in North America22. Recombinant RCN vaccines have been proven safe and effective when administered to various species like bats, domestic cats, piglets, sheep, prairie dogs, chickens, and non-human primates23,24,25,26,27,28,29.
In this study, we constructed two vaccine candidates, MVA-FG and RCN-FG, that co-express the NiV F and G proteins. We characterized the in vitro expression of these recombinant viruses and then quantified their humoral and cellular immune responses in mice. Furthermore, animals were vaccinated using multiple immunization routes to assess immunogenicity through mucosal delivery, a cornerstone for cost-effective, practical large-scale vaccine campaigns of the NiV reservoir host species.
Materials and methods
Cells and viruses
Chicken embryonic fibroblast cells (CEF; Charles River, Cat. No.: 10100807), baby hamster kidney cells (BHK-21; American Type Culture Collections (ATCC; Cat. No.: CCL-10), and African green monkey (Cercopithecus aethiops) kidney epithelial cells (Vero; ATCC, Cat. No.: CCL-81) were cultured in Dulbecco's modified Eagle media (DMEM; Corning, VA, Cat. No.: 90-113-PB), 5% fetal bovine serum (FBS; Gemini Bio-Products, Cat. No.: 50-753-2978), and antibiotics (1X Antibiotic-Antimycotic; Gibco, Cat. No.: 15240062) and incubated at 37 °C and 5% CO2. Wild-type (WT) MVA was obtained from BEI Resources (Manassas, VA, Cat. No.: NR-727); while the MVA-GFP strain used in this study was previously described by inserting green fluorescent protein (GFP) into the hemagglutinin gene of WT-MVA30,31. RCN wild-type (RCN-WT) was obtained from ATCC (Cat. No.: VR-838). Viruses were stored at − 80 °C until use. Viral titers were determined by a standard plaque assay32 and expressed as plaque-forming units per milliliter (PFU/mL).
Expression cassette designs
The MVA gene cassette (Fig. 1A) contained the F and G protein sequences from the 2004 Bangladesh strain of NiV (GenBank: AY988601). The strong pHyb promoter was placed in-frame with the full-length F gene containing a natural secretory signal to drive its expression, and the modified early/late PrH5m promoter (H5 gene from vaccinia virus) was used to control the expression of the G gene in frame with the tissue plasminogen activator (tPA) secretory signal33. The mCherry fluorescence marker (GenBank: ANF29837) and the xanthine guanine phosphoribosyl transferase (GPT) selection marker (Escherichia coli, GenBank: 75205729) were included to facilitate the selection of recombinant viruses.
For the RCN expression cassette (Fig. 1B), the F and G protein sequences from the 2018 NiV isolated during an outbreak in Kerala, India (GenBank: MH523642) were used. The F protein was fused with the human IgG secretory signal after removing the natural virus-derived secretory signal and expressed under the control of the PrMVA13.5L promoter (naturally occurring promoter in vaccinia virus; GenBank: MT227314). Similarly, the G protein was fused with the tPA secretory signal, and the hybrid PrS5E promoter was used to control its expression. The inclusion of the eGFP protein (GenBank: MN623123) permitted an easy distinction between wild-type (no fluorescence) and recombinant (green) viruses.
Protein sequences were back-translated, codon-optimized for vaccinia virus, and synthesized commercially (GenScript, Piscataway, NJ, USA). The MVA gene cassette was inserted into MVA-GFP as a gene knock-out for GFP as described previously30,31, while the RCN gene cassette was inserted upstream to the thymidine kinase (TK) gene of the RCN-WT.
Importantly, gene sequences used in the MVA-FG vaccine were derived from virus isolated from a human infected with NiV whereas gene sequences used in the RCN-FG vaccine were isolated from a bat infected with NiV. While sequences had high homology (99% amino acid homology in the F protein and over 95% amino acid homology in the G protein), difference sequences were used based on the target species for both vaccines and the limited data on how minor amino acid changes can affect antibody binding.
Production of recombinant viruses
In vitro production and amplification of recombinant viruses were performed in the BSL-2 lab. PCR-amplified DNA for each expression cassette was purified using a Zymoclean Gel DNA recovery kit (ZYMO Research, Irvine, CA, USA, Cat. No.: D4002). PCR amplicons were then encased in a lipid coat using FuGENE® HD reagent (Roche Diagnostics, Indianapolis, IN, USA, Cat. No.: E2311) and used to transfect MVA-GFP infected CEF cells and RCN-WT infected Vero cells as described previously34,35. Cells expressing mCherry (MVA) and GFP (RCN) were selected under a fluorescent microscope for five rounds of selection until complete plaque purification was confirmed through PCR35. A single plaque isolate was amplified to generate a high titer seed as previously described and underwent Sanger sequencing at the University of Wisconsin-Madison (UW-Madison) Biotechnology Center to ensure genetic stability35.
Immunofluorescence assay
A 12-well plate of either BHK-21 (MVA) or Vero (RCN) cells was infected with one multiplicity of infection (MOI) = 1 PFU/cell with MVA-FG and RCN-FG. The MVA-GFP and RCN-WT viruses were used as their corresponding negative controls. At 24 h (hr) post-infection (p.i.), cells were fixed with cold methanol/acetone (1:1) for 15 min (min) for intracellular protein expression or 4% paraformaldehyde for extracellular protein expression and then washed with 1X phosphate buffer saline (PBS). Cells measuring intracellular protein expression were permeabilized with 0.25% Triton X-100 in 1X PBS for 15 min at room temperature (RT) and included 0.05% Triton X-100 in the following antibody incubation and washing solutions. Cells were then washed with 1X PBS and blocked with 3% bovine serum albumin (BSA) in 1X PBS for 1 h at RT. After blocking, the primary antibody (in-house, 1:1000 dilution) in 3% BSA and 5% FBS was added to cells and incubated at RT for 2 h in the dark. Cells were then washed with 1.5% BSA in 1X PBS three times for 10 min and overlaid with the secondary antibody (goat-anti mouse Alexa Fluor IgG (H + L) highly cross-adsorbed plus 488, 1:3,000 dilution) in 3% BSA and 5% goat serum (Thermo Fisher Scientific, Waltham, MA) at RT for 1 h in the dark. Cells were washed with 1X PBS for 10 min three times and visualized under a fluorescent microscope (AMG EVOSfl, Thermo Fisher Scientific, Waltham, MA, USA).
Ethics statement
All mouse studies followed the guidelines described in the National Institute of Health's Guide for the Care and Use of Laboratory Animals36. All animals and their facilities were under the authority of the UW-Madison School of Veterinary Medicine and supervised by the UW-Madison Research Animal Resource Center. Protocols were approved by the UW-Madison Institutional Animal Care and Use Committee (IACUC; approval # V006189) and animal studies were conducted in accordance with the ARRIVE guidelines.
Mouse studies
While hamsters are typically used as a model for NiV animal studies, mice were chosen due to ease of handling and as challenge studies were not performed, a species susceptible to Nipah virus was not needed. BALB/c and AJ mice (4-week-old mixed sex) were purchased from Jackson Laboratory (JAX, Sacramento, CA, USA) and housed in a specific-pathogen-free room at the BSL-II Animal Health and Biomedical Sciences building per UW-Madison husbandry protocols. Mice were anesthetized prior to vaccination and blood collection via isoflurane inhalation in a closed-chamber system. Mice were divided into six groups, with eight mice of mixed sex per group (Fig. 2B). After an acclimatization period of 7 days, three groups received the MVA-FG vaccine (BALB/c mice), while the remaining three groups received the RCN-FG vaccine (AJ mice). Groups received one dose via subcutaneous injection (SC) or intranasal (IN) for each vaccine or two doses via SC 28 days apart (Fig. 2A). Viruses were diluted in 1 mM Tris–HCL, and mice were given a dose of 108 PFU for each immunization (50µL per mouse). At weeks 3, 5, and 7 after prime a submandibular blood puncture was performed using a 4 mm animal lancet. Samples were collected in MicrovetteⓇ 500 Z-Gel (Sarstedt, VWR, PA, Cat. No.: 103218-893), kept at RT for 15 min, and centrifuged at 10,000g at 20 °C for 5 min. Serum was allocated and stored at − 20 °C for ELISA and neutralization assays. After euthanizing the animals 7 weeks after prime, spleen and lung cells were collected to assess the level of F and G-specific cytokine-secreting cells via ELISpot. Daily observation for any changes in health status by UW-Madison veterinary car staff and weighing three times per week for 2 weeks were carried out after vaccination to monitor changes in the animal’s body condition.
For flow cytometry studies, vaccines were prepared as described previously, and two doses of 108 PFU for each immunization were given four weeks apart (Fig. 3A). BALB/c mice were divided into three groups, with four mice per group receiving MVA-FG via SC and IN, and MVA-GFP via SC. AJ mice were similarly divided and received RCN-FG via SC and IN, and RCN-WT via SC (Fig. 3B). Serum was collected 7 weeks after prime to assess the humoral immune response of boosted IN groups via ELISA. At 8 weeks post-prime, T cell memory responses were measured in spleen and lung cells and a bronchoalveolar lavage was performed to evaluate the level of F and G specific IgG, IgA, and IgM antibodies in lung tissues.
Enzyme-linked immunosorbent assay (ELISA)
Immulon 2HB high-binding ELISA plates (Thermo Scientific, Waltham, MA, Cat. No.: 3455) were coated with 100 µg/well of either NiV fusion protein (gF) exodomain (Native Antigen, Kidlington, Oxford, UK, SKU: REC31632) or NiV glycoprotein G (gG) exodomain (Native Antigen, Kidlington, Oxford, UK, SKU: REC31637) diluted in Carbonate-Bicarbonate buffer (Sigma, Saint Louis, MO, Cat. No.: C3041) and incubated overnight at 4 °C. Plates were blocked in either 5% non-fat dry milk (for NiV-G) or 2% BSA (for NiV-F) in PBS-T for 1 h at 37 °C. Naive serum was used as a negative control, and antiserum produced in-house was used as a positive control. Serum samples were diluted in their respective blocking buffers with 5% FBS using three-fold dilutions (1:50 primary dilution) and incubated at 37 °C for 1 h. Serum and BALF samples were incubated with goat anti-mouse IgG (H + L) peroxidase conjugate (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No.: 31430). BALF samples were also incubated with a goat anti-mouse IgA HRP secondary antibody (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No.: 62-6720) and a goat anti-mouse IgM (Heavy chain) HRP secondary antibody (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No.: 62-6820). Secondary antibodies were diluted in their respective blocking buffers with 5% goat serum (1:2,000 dilution) and incubated for 1 h at 37 °C. 1-Step ABTS substrate solution (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No.: 37615) was added, and plates were incubated at RT for 15 min followed by a 1% SDS stop solution (Promega, Madison, WI, USA, Cat. No.: V6551). Plates were read at 405 nm and 650 nm no more than 30 min after visualizing. Reciprocal values of the endpoint dilution were considered positive if they measured greater than the mean value of a naive serum sample plus three times the standard deviation per plate.
Neutralization assay
For neutralization assays, serum collected during the vaccination study was sent to the US Centers for Disease Control and Prevention (CDC) in Atlanta, Georgia, USA. Serum samples were heat-inactivated at 56 °C for 30 min before being diluted in two-fold dilutions in duplicate (1:40 to 1:5,120). Diluted serum was incubated with 100–150 TCID50/well of the Bangladesh NiV strain isolate 810398 (GenBank accession MK673564.1) for 1 h at 37 °C in the BSL-4 and then transferred into confluent Vero cells37. Wells were examined for cytopathic effects (CPE) after 6 days p.i. Reciprocal dilution titers were considered neutralizing at the lowest dilution in which both duplicates showed no CPE. Naive sera from BALB/c and AJ mice were tested as negative controls, and rabbit anti-Hendra soluble G serum was used as a positive control (neutralizing up to a reciprocal dilution of 640)38. Anti-Hendra G serum was used as a positive control due to its ability to elicit neutralizing antibody responses against both Nipah and Hendra viruses39. Viruses were back-tittered in duplicate for virus stocks to confirm intended inoculum amount.
Spleen, lung, and BALF harvesting
Spleens were removed from freshly euthanized mice, connective tissue was removed, and were rinsed with Hanks balanced salt solution (HBSS, Sigma Aldrich, Darmstadt, Germany, Cat. No.: H9394) in a 35 mm culture dish. Lung tissue was minced in a 35 mm culture dish and digested for 30 min with digestion buffer (Collagenase D (Sigma Aldrich, Darmstadt, Germany, SKU: 11088858001), DNase I (New England Biolabs, Cat. No.: M0303S), FBS, 4 mM glutamine, 25 mM HEPES, and antibiotics in HBSS). Spleens and lungs were crushed using the plunger of a 3 cc syringe and filtered through a 70 µm cell strainer with 1X PBS. Cells were centrifuged at 300g for 10 min at 4 °C. The supernatant was removed, and cells were resuspended in a red blood cell lysis buffer (0.85% NH4Cl and 0.23% Tris in water) and incubated for 5 min. Cells were centrifuged at 300g for 10 min at 4 °C. Cells were resuspended to 3 × 106 cells/mL in CTL-Test™ media (CTL, ImmunoSpot, OH, USA, Cat. No.: CTLT-010) supplied from single-color murine TNF-ɑ and double color murine IFN-γ/IL-2 ImmunoSpot® kits from Cellular Technology Ltd. (CTL, ImmunoSpot, OH, USA).
Following humane euthanasia, mice were pinned, and the neck was disinfected with 70% ethanol. An incision was made at the neck, and skin was pulled back to expose the trachea. A 20 G catheter needle was then inserted above the cartilage rings of the trachea to a depth of 0.5 cm. A 1 mL syringe loaded with 1% FBS in Roswell Park Memorial Institute medium (RPMI) was injected into the lungs, and the aspirated fluid was collected in a 15 mL conical tube. After repeating this process three times, the BALF fluid was centrifuged at 800g for 3 min at 4 °C. The supernatant was collected and stored at − 20 °C until analysis via ELISA.
ELISpot IFN-γ, IL-2, and TNF-α analysis
Spleen and lung cells were added in duplicate with 300,000 cells/well to high-protein-binding PVDF 96-well plates primed with murine IFN-γ/IL-2 capture solution or TNF-ɑ capture solution according to the manufacturer's protocol. Purified NiV-F (Native Antigen) and NiV-G (Native Antigen) antigens were added at 0.2 µg/well in duplicate and incubated at 37 °C for 48 h. Plates were then washed, incubated with corresponding cytokine detection solutions, and developed according to the manufacturer's protocol. Spots were scanned with ImmunoSpot software (Version 7.0.15.2) and counted using the open-source software Viridot40.
T cell memory assessed by flow cytometry
Splenocytes and lung cells were counted and seeded at around 2 × 106 cells/well into 96-well round-bottom plates. Plates were centrifuged at 800g for 3 min at RT, and the supernatant was discarded. For each tissue type and animal, the cells were stimulated with 200 µL/well of an F protein-peptide pool (13-mers with 2 amino acid overlap, ThermoFisher), a G protein-peptide pool (13-mers with 2 amino acid overlap, ThermoFisher), a cell stimulation cocktail (TONBO, Cat. No.: TNB-4975) as a positive control, or dimethyl sulfoxide (DMSO)/RPMI-complete medium (Lonza, Cat. No.:BE12-115F) as a negative control. Plates were incubated for 5 h at 37 °C, 5% CO2. After incubation, plates were centrifuged at 800g for 3 min at 4 °C and washed with 1X PBS where supernatant was discarded. A 1:1000 live/dead cell stain (Aqua-Dye, ThermoFisher) diluted in 1X PBS was mixed with cells and incubated for 30 min at 4 °C in the dark. Cells were then washed with FACS buffer (1X PBS, 0.5% BSA, 0.1% sodium azide) and centrifuged at 800g for 3 min at 4 °C, where the supernatant was discarded. Surface staining antibodies (Supplementary Table 1) were added and incubated at 30 min at 4 °C. Cells were then washed with FACS buffer, and cells were fixed and permeabilized (BD Cytofix/Cytoperm, BD Biosciences, Cat. No.: 554722) for 30 min at 4 °C. Cells were then washed with Permwash, and cells were resuspended in 200 µL of FACS buffer, transferred into a FACS tube, and read using a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA, USA).
Flow cytometry gating strategy
Compensation was done using UltraComp eBeads Compensation Beads (ThermoFisher, Cat. No.: A10344) and an ArC Amine Reactive Compensation Bead Kit (Thermo Fisher Scientific, Cat. No.: 01–2222-42). Data were analyzed using FlowJo software (BD Biosciences, TreeStar, Ashland, OR, USA) and an automated flowClean FlowJo plugin41. Gating strategies (Supplementary Fig. 1) excluded dead cells using a cell viability stain (Aqua Dye), doublet exclusion via forward scatter height (FCS-H) and forward scatter area (FCS-A) dot plot and selected for proliferating CD4+ and CD8α+ T cells using a forward (FSC) and side scatter (SSC) dot plot. Live, CD4+ and CD8α+ lymphocytes were then gated positive at the FITC axis and the BUV395 axis, respectively, in a FITC-BUV395 dot plot. Similar approaches were used to identify populations of CD4+ and CD8α+ T cells expressing cell surface markers. Samples were excluded from analysis for tissue samples yielding non-viable cell isolates.
Statistical Analysis
Statistical analysis was performed between vaccine delivery groups for antibody titers using a one-way Kruskal Wallis Analysis of variance (ANOVA) and assessed using a Dunn’s multiple comparison test upon discovery of significant differences. Flow cytometry analysis was conducted using a one-way ANOVA and a Tukey’s multiple comparison test after the discovery of significant differences. All data were analyzed with GraphPad Prism version 9.1.1 software for Mac, GraphPad Software, San Diego, California USA, www.graphpad.com. P-values < 0.05 (alpha) were considered statistically significant.
Results
Production and in vitro characterization of viral vector vaccines
The presence of the F and G genes, including the mCherry and eGFP genes, with their corresponding promoters and regulatory sequences, was confirmed via PCR and cell culture for both MVA-FG and RCN-FG virus constructs. DNA sequencing indicated no genetic alterations in the final virus stocks (data not shown).
We assessed the expression and cellular localization of the F and G proteins by immunofluorescence microscopy of MVA-FG and RCN-FG-infected cells (Fig. 4). F protein expression was identified in permeabilized (Fig. 4A1) and nonpermeabilized (Fig. 4B1) CEF cells infected with the MVA-FG virus but not in the MVA-GFP-infected control cells. G expression was detected intracellularly in MVA-FG infected cells (Fig. 4A3), but no signal was observed on the cell membranes (Fig. 4B3) or in MVA-GFP-infected cells. However, western blot analysis demonstrated that the G protein is secreted into the supernatant (Supplementary Fig. 2D Lane 3). For the RCN-FG virus, the expression of the F (Fig. 4C1 and D1) and G (Figure 4C3 and D3) proteins is observed intracellularly and on the cell surface; no fluorescence is detected in RCN-WT-infected Vero cells. Western blot analysis (Supplementary Fig. 3) confirms protein expression of the F and G proteins intracellularly and extracellularly for RCN-FG infected cells while RCN-WT infected Vero cells lack these protein bands. Raw western blots for MVA-infected cells and RCN-infected cells can be found in Supplementary Figs. 4 and 5, respectively.
While the RCN-FG vaccine displayed the formation of syncytia and both F and G protein localization on the cell membrane was confirmed via immunofluorescence, the same could not be said for the MVA-FG vaccine. It is hypothesized based on the lack G protein localization via immunofluorescence, lack of syncytia formation in infected cells, and the detection of the G protein in the western blot, that the G protein is being secreted into the supernatant and is not membrane-anchored for the MVA-FG vaccine.
Post-immunization safety monitoring of mice vaccinated with MVA-FG and RCN-FG
Mice were vaccinated with one or two doses via SC and one dose via IN immunization with 108 PFU of either MVA-FG or RCN-FG vaccine candidates. Animals were weighed three times per week for a period of two weeks and monitored daily for any changes in health status by UW-Madison animal care staff. Mouse body weights post-vaccination (p.v.) were compared to the baseline weight taken immediately prior to vaccination. Interestingly, the only group that showed a decrease in weight were mice immunized IN with MVA-FG (Fig. 5). MVA-FG IN mice lost less than 5% of body weight compared to their baseline weight following vaccination until day 5 p.v. After 5 days p.v, MVA-FG IN mice began increasing in weight until day 10 where they surpassed their initial baseline weight. RCN-FG vaccine groups and MVA-FG groups vaccinated SC had a steady increase in weight p.v. throughout the two-week period. The limited weight loss p.v. and no adverse health events reported by veterinary care staff corroborate previous viral vector safety data and provide initial safety data for these vaccine candidates to undergo further studies for rigorous safety testing.
NiV-F and NiV-G specific antibody levels induced in mice
Mice were vaccinated with either MVA-FG or RCN-FG viral vectors using one or two doses via SC injection or one dose via IN injection (Fig. 2). The reciprocal endpoint IgG antibody titers were measured using ELISA from serum samples collected on weeks 3, 5, and 7 (Fig. 6). Antibody titers were expressed as the logarithm base 10 geometric mean titer (GMT) per group. In terms of anti-F antibody titers (Fig. 6A), there were no statistical differences between all MVA-vaccinated groups. All three groups generally induced low levels at week 3, which increased when measured at weeks 5 and 7. Specifically, the SC boost group showed higher F titer levels at weeks 5 and 7 than the SC prime and IN groups.
In contrast, RCN-vaccinated groups displayed differences in F antibody titers (Fig. 6A). Overall, the IN group consistently induced higher levels at all three-time points than the other two groups. On the other hand, both SC groups showed an increase in titer levels from week 3 to week 7, but lower than IN groups. Statistical differences at weeks 3 and 5 indicated that the IN group induced higher levels than SC prime and SC boost at week 3. However, there were no differences between IN and SC prime groups at week 7 and between IN and SC boost at weeks 5 and 7. Notably, F titers from animals immunized with RCN-FG IN were higher than levels observed in all three MVA-vaccinated groups (Fig. 6A).
Overall, the G-specific antibody titers induced in mice vaccinated with MVA-FG or RCN-FG (Fig. 6B) were higher than antibody titers seen against the F protein. BALB/c mice immunized with the MVA-FG vaccine showed statistically comparable G antibody titers among all MVA-vaccinated groups but with a higher mean antibody titer seen in groups vaccinated SC versus IN.
High G antibody titers were achieved at all three weeks tested for the IN-treatment group for RCN-FG vaccinated AJ mice. Interestingly, titer levels induced in the SC boost group peaked at week 5 and were similar to the IN group but showed a reduction at week 7 comparable to levels obtained at week 3. In contrast, the SC prime group peaked at week 3 but gradually induced lower levels at weeks 5 and 7. F specific antibody titers for MVA-FG and RCN-FG IN boosted mice (Fig. 6C) saw an increase at 7 weeks post-prime compared to mice receiving a single dose. Antibody titers against the G protein showed comparable levels between mice receiving one or two doses of either the MVA-FG or the RCN-FG vaccines.
In the flow cytometry T cell memory study (Fig. 3), serum was collected from BALB/c and AJ mice vaccinated with two doses of either MVA-FG or RCN-FG IN four weeks apart and the levels of circulating antibodies 7 weeks after prime for IN groups were measured. F antibody titers in MVA-FG and RCN-FG vaccinated mice showed an increase in the mean antibody titer following an IN boost, while G mean antibody titers remained comparable to levels after a single dose for both MVA-FG and RCN-FG-vaccinated mice.
Both vaccines produced high levels of circulating antibodies against both the F and the G glycoproteins and were assessed based on vaccine administration route. Mice vaccinated with MVA-FG did not have antibody titer differences when using different administration routes suggesting route of administration could be variable. Unlike MVA-FG vaccinated mice, RCN-FG vaccinated mice did show differences in antibody production levels based on vaccine administration route, suggesting the IN vaccine delivery route would outperform the SC route in generating circulating antibodies.
Neutralizing antibodies induced in mice
Mouse serum collected on weeks 3, 5, and 7 was evaluated for the presence of nAbs against the NiV Bangladesh strain (Fig. 7). Serum was tested in duplicate for 100% neutralization via CPE visualization 6 days p.i. Reciprocal dilution titers were considered neutralizing at the lowest dilution in which both duplicates showed no CPE. Although none of the vaccine candidates induced nAbs at week 3 after the first immunization, in the MVA-FG vaccinated animals, the SC boost group induced the highest mean titer of nAbs, which increased from week 5 (1:159.5) to week 7 (1:226.3). The MVA-FG SC prime reached a final titer on week 7 (1:72.4), while IN group peaked at week 5 (1:42.7). RCN-vaccinated mice had the highest overall titer of nAbs in the IN prime group on weeks 5 (1:495) and 7 (1:419), followed by the RCN-FG SC boost group, which reached 133.7 in week 5 and 146.3 in week 7, and RCN-FG SC prime which reached a final titer of 66.6 on week 7.
Overall, both vaccines were able to induce neutralizing antibodies. For mice vaccinated with MVA-FG, the level of neutralizing antibodies produced was dependent on vaccine administration route with the SC groups having significantly higher neutralizing antibodies the IN group. Furthermore, mice vaccinated SC also showed an increase in the level of neutralizing antibodies generated with two doses versus a single dose. Therefore, while circulating antibodies were unaffected by vaccine administration route, the ability of the antibodies to neutralize the virus was affected. Mice vaccinated with RCN-FG had differences like the overall level of circulating antibodies seen in serum. The RCN-FG IN group had significantly high levels of neutralizing antibodies generated quickly compared to SC administration, affirming the IN group as the better vaccine administration route.
IFN-γ, IL-2, and TNF-α secreting cells stimulated by NiV-F and G glycoproteins
Splenocytes (Fig. 8A) and lung cells (Fig. 8B) collected from BALB/c and AJ mice 3 weeks post-boost or 7 weeks post-prime were analyzed for IFN-γ, IL-2, and TNF-α cytokine secreting cells in response to stimulation by the full-sized NiV-F and G glycoproteins. The MVA-FG SC and IN groups showed high levels of all three cytokines. Based on vaccine administration route, MVA-FG IN groups had higher levels of TNF-⍺, IFN-γ, and IL-2-secreting cells in splenocytes compared with groups receiving MVA-FG SC for both the F and the G antigen. SC primed and boosted groups showed similar levels of TNF-⍺, IFN-γ, and IL-2 cytokine-secreting cells for both cell types when stimulated with the F and G antigens.
RCN-FG vaccinated mice had low levels of TNF-⍺, IFN-γ, and IL-2 cytokine-secreting cells in both splenocytes and lung cells against both the F and G antigen. However, a higher percentage of TNF-⍺ secreting lung cells was detected against the G antigen compared with splenocytes for mice vaccinated SC. For mice vaccinated IN, similar percentages of TNF-⍺ secreting cells were detected in both splenocyte and lung cells against the G antigen.
Cytokine-secreting cells in mice vaccinated with MVA-FG had the highest level of expression via IN vaccine route, with SC groups showing no significant difference between SC groups that received one or two doses. RCN-FG vaccinated mice did not show significant differences between vaccine administration groups, however a higher percentage of TNF-⍺ secreting cells was noted in splenocytes for mice vaccinated IN.
T cell memory assessed via flow cytometry
BALB/c and AJ mice were vaccinated with the MVA-FG or the RCN-FG vaccine SC and IN in two-dose schedule 4 weeks apart. Additional groups received an MVA-GFP or an RCN-WT virus SC at vector controls. One naive mouse from each species was kept as naive control. Five weeks post-boost, mouse splenocytes, and lungs were collected from BALB/c and AJ mice and analyzed for effector (TEM), central (TCM), and resident tissue (TRM) CD4+ (Supplementary Fig. 6) and CD8⍺+ T cells (Fig. 9).
Splenocytes from MVA-vaccinated BALB/c mice (Fig. 9A) had significant effector memory expression in MVA-FG IN groups compared to MVA-FG SC (p < 0.0001), and naive mice (p < 0.0001). Mice vaccinated with MVA-GFP SC also showed significant expression of effector memory compared to naive mice (p = 0.0053). For central and resident memory CD8⍺+ T cells, no significant differences were found, however, MVA-FG IN mice had the highest level of expression compared to MVA-FG SC, MVA-GFP SC, and naive mice. Lung cells (Fig. 9B) were collected from BALB/c mice vaccinated with MVA-FG SC and IN and MVA-GFP SC with low cell collection in naive mice being excluded from the data set. MVA-FG IN mice had the highest expression of all memory CD8⍺+ T cell subsets in the MVA-FG IN group, significantly greater than the MVA control SC group for effector memory expression (p = 0.0455). MVA-FG SC groups also saw some effector, central, and tissue resident CD8⍺+ T cell expression, with little to no expression seen in MVA-GFP SC groups.
Splenocytes collected from AJ mice (Fig. 9C) had significantly high expression of TEM in RCN-FG SC mice compared to RCN-WT SC (p = 0.0264) and naive AJ mice (p = 0.0002). Mice vaccinated with RCN-FG IN (p = 0.0205) and RCN-WT SC (p = 0.0470) also showed significant effector memory CD8⍺+ expression compared with naive mice. While no significant differences were seen for central or tissue resident memory CD8⍺+ splenocytes, RCN-FG SC and IN mice had the higher expression of both phenotypes compared to RCN-WT SC vaccinated mice and naive AJ mice. For lung cells (Fig. 9D) from AJ mice, no significant differences were seen between groups for CD8⍺+ T cell memory subsets, however higher levels of expression was seen in mice vaccinated with RCN-FG SC and IN.
Importantly, both vaccines generated CD8ɑ+ TEM, TCM, and TRM responses in lung and spleen cells from vaccinated mice upon stimulation from peptides from the F and G proteins. While the MVA-FG vaccine generated the highest level of circulating antibodies when given SC, the highest phenotypic expression of effector, central, and tissue resident CD8ɑ+ T cells in both splenocytes and lung cells was detected when given intranasally which should be explored in future studies. Excitingly, the RCN-FG vaccine candidate generated effector and tissue resident CD8ɑ+ T cells via IN and SC administration in both splenocytes and lung cells supporting oral vaccination as a good means of immune stimulation using this vaccine.
Mucosal antibodies assessed via ELISA
BALF was collected from BALB/c and AJ mice 5 weeks post-boost, and the F and G antibody titers were assessed for IgG, IgA, and IgM antibody types to see if antibodies were generated in the respiratory tract along the natural route of infection. F-specific IgG antibodies (Fig. 10A) were detected in the RCN-FG IN group at a GMT of 125 which was significantly higher than the RCN-FG SC (p = 0.0032) and the RCN-WT SC (p = 0.0032) groups which had no detectable antibodies. For F-specific IgA (Fig. 10B), the RCN-FG IN group was the only group that had any detectable IgA antibody titers with a geometric mean of 50. For F-specific IgM (Fig. 10C), no antibodies against the F protein were detected in vaccine or vector control groups. IgG antibody titers against the G protein (Fig. 10D) had a GMT of 13.25 in MVA-FG SC groups, and 187.8 in MVA-FG IN groups, with no detectable antibodies in the MVA vector control group. For AJ mice, IgG antibody titers had a GMT of 1575 in the RCN-FG IN group which was significantly higher than the RCN-FG SC (p = 0.0155) and RCN-WT SC group (p = 0.0155). No IgA (Fig. 10E) or IgM (Fig. 10F) G-specific antibodies were detected for any vaccine or vector control groups in AJ mice. As hypothesized, neither vaccine produced mucosal antibodies when vaccinated subcutaneously with one or two doses, however, while neither vaccine produced F or G-specific IgM antibodies, both vaccines had detected IgG antibody titers for intranasally vaccinated mice. Notably, high G-specific antibody titers were seen for both vaccines and the generation of F-specific IgG and IgA antibodies were detected for mice vaccinated with RCN-FG. Interestingly, while F-specific circulating antibodies were produced, no F-specific antibodies were detected in respiratory tract of MVA-FG IN groups.
Discussion
While vaccine candidates against NiV are in development, none are commercially available for this fatal disease. This study assessed the immunogenicity and safety of poxvirus-based vaccines against NiV. The MVA-FG and RCN-FG vaccines induced high levels of anti-F and G circulating antibodies through subcutaneous and intranasal administration. Furthermore, BALF collected from vaccinated mice had detectable F-specific IgG and IgA antibodies in the RCN-FG IN group and G-specific IgG antibodies in the MVA-FG and RCN-FG IN groups. Most importantly, each vaccine generated high neutralization activity against the NiV Bangladesh (NiVB) strain. A single dose of the RCN-FG vaccine candidate, given intranasally, successfully neutralized 100% of NiVB at a mean dilution of 1:495, while the MVA-FG vaccine given via boosted SC route neutralized 100% of NiVB at a dilution of 1:240. While challenge studies were not conducted in this study, studies have shown that nAb levels are predictive of immune protection in various passive immunization studies in NiV animal challenge models15,42; however, challenge studies will need to be conducted to assess protection and efficacy.
Regarding the cellular immune response, splenocytes and lung cells harvested from mice vaccinated with MVA-FG and RCN-FG vaccine candidates secreted TNF-⍺, IFN-γ, and IL-2 in response to F and G protein stimulation. MVA-vaccinated mice had higher levels of cytokine expression compared to RCN-vaccinated mice. MVA-FG mice vaccinated IN had the highest expression of TNF-⍺, IFN-γ, and IL-2 in splenocytes. Furthermore, CD4+ and CD8ɑ+ T cell memory responses showed the generation of TEM, TCM, and TRM in both MVA-FG and RCN-FG vaccinated mice. Specifically, the MVA-FG vaccine given intranasally saw the highest phenotypic expression of effector, central, and tissue resident CD8ɑ+ T cells in both splenocytes and lung cells compared to vector control and naive mice. The RCN-FG vaccine candidate generated high effector memory CD8ɑ+ T cells when given SC and produced tissue resident CD8ɑ+ T cells via IN administration in splenocytes. In lung cells, the RCN-FG vaccine showed higher effector, central, and tissue resident CD8ɑ+ T cell expression compared with RCN-WT SC and naive mice. Again, while challenge studies would need to be conducted to assess protection, cytokine secretion and the generation of TEM, TCM, and TRM in both spleen and lung cells are comparable to levels seen in swine challenge studies that confer protection43.
Recombinant poxvirus vectors are easily constructed and provide high immunogenicity and enhance the induction of cellular and humoral-mediated immune responses44. Furthermore, unlike some viral vectors, poxviruses do not carry the risk of DNA recombination in target cell genomes due to the characteristic of cytoplasmic replication. While the consideration of vector immunity is an important area of focus, studies have shown that a single recombinant poxvirus can successfully express transgenes after multiple immunizations45. It is hypothesized that the antibodies produced against poxvirus target the virion during release from infected cells and not the entry/attachment phase at initial infection45. With this is mind, people recently infected with poxvirus would generate antibodies that prevent cell-to-cell spread of the viral vectors at the entry/attachment phase45. With one of the last known cases of smallpox in Bangladesh and the deployment of the MVA vaccine to counteract recent monkeypox infections in humans, long-term vector immunity should be studied in greater depth46. In addition, the poxvirus viral vectors used as the delivery platform for the NiV F and G proteins are safe and effective18,19,20,21,23,24,25,26,27,28,29. In wildlife, both the MVA and RCN viral vectors are safe and effective in bat and pig populations47,48. In this study, mice vaccinated with the MVA-FG and RCN-FG vaccines saw limited weight loss post-vaccination suggesting an initial safe vaccine profile. However, further preliminary data would need to be collected to determine the final safety profile prior to clinical trials or animal challenge studies such as cytokine production, and monitoring for immediate or long-term adverse events.
Additionally, the MVA and RCN viral vectors elicit high humoral immune responses in bats through oral immunization necessary for practical, wide-scale animal vaccinations in the field49. The RCN vectored vaccine presents a promising wildlife vaccine against NiV in both the bat reservoir and pigs, an amplifying host of NiV, to reduce the spread from wildlife to human populations and prevent the economic disruption caused by swine exportation to areas without the competent host reservoir. By being able to vaccinate both humans and wildlife, the MVA vaccine candidate presents a possible One-Health vaccine candidate and creates an avenue for disease eradication.
Current vaccine approaches for Nipah rely on subunit, mRNA, VLP, DNA, or viral vector strategies48,49,50,51,52,53,54,55,56,57. Currently, an Equivac® HeV recombinant subunit vaccine, licensed for horses in Australia against Hendra virus and NiV, is undergoing phase I clinical trials for humans48,58. Like the vaccines proposed in this study, the Equivac® HeV vaccine focuses on a One-Health approach against Hendra virus by targeting the transmission from horses to humans59. While this vaccine has proven to protect both ferrets and African green monkeys from fatal NiV challenges, only partial protection was obtained in pigs due to their requirement of both cellular and humoral immune responses for complete protection60. Recombinant vectored vaccines have many promising candidates in development tailored toward both humans and pigs including vaccines such as the recombinant rabies virus (NIPARAB), a vesicular stomatitis virus (rVSV-ΔG-NiVBG), canarypox virus (ALVAC-F/G) vaccines, and ChAdOx1 NiVB, a simian adenovirus vectored vaccine53,61,62,63. Although promising, vaccine candidates against NiV fail to include the reservoir host as a vaccine target, but instead focus on the transmission between humans and swine. Due to the infrequent nature of NiV outbreaks and limited wide-scale marketability, vaccination campaigns may focus on rapid vaccine deployment in outbreak regions64. By deploying a vaccine capable of use in the reservoir host, wildlife vaccination can focus on preventing the spread of NiV between migratory bat regions and to other susceptible species such as pigs and humans during seasonal outbreaks. While vaccine models have been able to induce protective immune responses, few vaccine candidates (RABV and VSV) have tested protective efficacy via intranasal administration, significantly reducing their practical application in animal populations that require mucosal delivery. For current vaccine models to fully target the transmission cycle of NiV, they would need to entail a collaborative effort to halt transmission between the reservoir host, the amplifying host, and humans. Without a One Health-based vaccine approach capable of targeting the transmission cycle of NiV, control and prevention focus on mitigation after an outbreak occurs. Since NiV persists in bat reservoirs, this reservoir must be targeted through vaccine strategies to prevent wide-scale outbreaks.
These results present two promising poxvirus vaccine candidates against NiV that may provide an avenue for protection in both humans and animals involved in NiV transmission. The stimulation of high humoral immune responses through both subcutaneous and intranasal routes highlights their potential as wildlife vaccines in humans, swine, and the reservoir bat species. Finally, the induction of CD8+ T cell responses emphasizes the induction of cellular immune responses required for complete protection against NiV in swine species.
Data availability
All data are available within the manuscript, supplementary material, or at the corresponding author’s request. MVA-FG (https://www.ncbi.nlm.nih.gov/nuccore/OQ352141) and RCN-FG (https://www.ncbi.nlm.nih.gov/nuccore/OQ352142) DNA construct sequences are available online through GenBank (Accession OQ352141.2 and OQ352142.2, respectively). Information regarding the NiV Bangladesh strain (Accession MK673564.1) used for virus neutralization assays is available through GenBank (https://www.ncbi.nlm.nih.gov/nuccore/1802790317).
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Acknowledgements
This work is funded through the United States Department of Agriculture Animal Plant Health Inspection Service's National Bio- and Agro-defense Facility Scientist Training Program. Special thanks to Elsa Cárdenas Canales who assisted with tissue collections. The findings and conclusions in this report are those of the authors and do not necessarily represent those of the Centers for Disease Control and Prevention. This work was supported in part by CDC core funding.
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Conceptualization, E.S.M.M., J.E.O., J.L.M.; methodology, E.S.M.M., J.E.O., J.L.M., M.K.L; formal analysis, E.S.M.M., J.L.M.; investigation, E.S.M.M., J.E.O., J.L.M., L.G.M.M., C.S.C., M.K.L; resources, J.E.O., J.M.M; data curation, E.S.M.M.; writing—original draft preparation, E.S.M.M., J.E.O., J.L.M., L.G.M.M.; writing—review and editing, E.S.M.M., J.E.O., J.L.M., L.G.M.M., C.S.C., C.F.S, J.M.M, M.K.L; visualization, E.S.M.M.; supervision, J.E.O., J.L.M., C.F.S., J.M.M; funding acquisition, E.S.M.M., J.E.O., J.M.M.. All authors have read and agreed to the published version of the manuscript.
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J.E.O. is the CEO of VaxThera. All remaining authors declare no competing interests.
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Medina-Magües, E.S., Lopera-Madrid, J., Lo, M.K. et al. Immunogenicity of poxvirus-based vaccines against Nipah virus. Sci Rep 13, 11384 (2023). https://doi.org/10.1038/s41598-023-38010-2
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DOI: https://doi.org/10.1038/s41598-023-38010-2
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